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产品概述
小鼠肠巨噬细胞
Cat NO.: CP-M048
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产品名称：小鼠肠巨噬细胞
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组织来源：肠组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
小鼠肠巨噬细胞分离自肠道组织；肠道指的是从胃幽门至肛门的消化管。肠是消化管中最长的一段，也是功能最重要的一段。哺乳动物的肠包括小肠、大肠和直肠3大段。大量的消化作用和几乎全部消化产物的吸收都是在小肠内进行的，大肠主要浓缩食物残渣，形成粪便，再通过直肠经肛门排出体外。肠道堪称身体最劳累的器官——每天不停地消化、吸收食物，以提供足够的养分，其实它的功能还远不止此——它还是机体内最大的微生态系统。巨噬细胞属于免疫细胞，有多种功能，是研究细胞吞噬、细胞免疫和分子免疫学的重要对象。巨噬细胞属免疫细胞，有多种功能，是研究细胞吞噬、细胞免疫和分子免疫学的重要对象。巨噬细胞较易获得，便于培养，并可进行纯化。巨噬细胞属不繁殖细胞群，在条件适宜下可生活2-3周，多用做原代培养，难以长期生存。巨噬细胞(Macrophages)是一种位于组织内的白血球，源自单核细胞，而单核细胞又来源于骨髓中的前体细胞。巨噬细胞和单核细胞皆为吞噬细胞，在脊椎动物体内参与非特异性防卫(先天性免疫)和特异性防卫(细胞免疫)。它们的主要功能是以固定细胞或游离细胞的形式对细胞残片及病原体进行噬菌作用(即吞噬以及消化)，并激活淋巴球或其他免疫细胞，令其对病原体作出反应。
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方法简介：
普诺赛实验室分离的小鼠肠巨噬细胞采用酶消化法结合差速贴壁法制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的小鼠肠巨噬细胞经CD68免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-M048 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 巨噬细胞样 传代特性 属于终末分化细胞；属于不增殖细胞群 消化液 利多卡因(12mM) 小鼠肠巨噬细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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M1 intestinal macrophages-derived exosomes promote colitis progression and mucosal barrier injury (2024-03-26)
作者：Rui Du:1.College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China. ; Sihan Chen:1.College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China. ; Chenyang Han:1.Department of Pharmacy, The Second Affiliated Hospital of Jiaxing University, Jiaxing 314001, China. ; Zhongmei He:1.College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China. ; Hongyan Pei:1.College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China. ; Yang Yang:1.Department of Cardiology, Shenyang Medical College Affiliated Second Hospital, Shenyang 314005, China. ;
期刊：M1 intestinal macrophages-derived exosomes promote colitis progression and mucosal barrier injury
影响因子 ：5.2
引用产品： 小鼠肠巨噬细胞
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Th1 promotes M1 polarization of intestinal macrophages to regulate colitis-related mucosal barrier damage (2023-04-04)
作者：Shuiliang Ruan:1.The Second Affiliated Hospital of Jiaxing University, Jiaxing 314001, Zhejiang, China. ; Liang Xu:1.Jiaxing University Master Degree Cultivation Base, Zhejiang Chinese Medical University, Hangzhou 310000, Zhejiang, China. ; Yongjia Sheng:1.The Second Affiliated Hospital of Jiaxing University, Jiaxing 314001, Zhejiang, China. ; Jin Wang:1.The Second Affiliated Hospital of Jiaxing University, Jiaxing 314001, Zhejiang, China. ; Xiaohong Zhou:1.The Second Affiliated Hospital of Jiaxing University, Jiaxing 314001, Zhejiang, China. ; Caiqun Zhang:1.The Second Affiliated Hospital of Jiaxing University, Jiaxing 314001, Zhejiang, China. ; Li Guo:1.The Second Affiliated Hospital of Jiaxing University, Jiaxing 314001, Zhejiang, China. ; Wenyan Li:1.The Second Affiliated Hospital of Jiaxing University, Jiaxing 314001, Zhejiang, China. ; Chenyang Han:1.The Second Affiliated Hospital of Jiaxing University, Jiaxing 314001, Zhejiang, China. ;
期刊：Th1 promotes M1 polarization of intestinal macrophages to regulate colitis-related mucosal barrier damage
影响因子 ：5.2
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MicroRNA-9a-5p-NOX4 inhibits intestinal inflammatory injury by regulating the M1 polarization of intestinal macrophages (2022-10-24)
作者：Wenyan Li:1.Department of Pharmacy, The Second Affiliated Hospital of Jiaxing University, Jiaxing, China. ; Yongjia Sheng:1.Department of Pharmacy, The Second Affiliated Hospital of Jiaxing University, Jiaxing, China. ; Jin Wang:1.Department of Pharmacy, The Second Affiliated Hospital of Jiaxing University, Jiaxing, China. ; Shasha Wu:1.Department of Pharmacy, The Second Affiliated Hospital of Jiaxing University, Jiaxing, China. ; Chenyang Han:1.Department of Pharmacy, The Second Affiliated Hospital of Jiaxing University, Jiaxing, China. ;
期刊：MicroRNA-9a-5p-NOX4 inhibits intestinal inflammatory injury by regulating the M1 polarization of intestinal macrophages
影响因子 ：3.6
引用产品： 小鼠肠巨噬细胞
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Moronic acid improves intestinal inflammation in mice with chronic colitis by inhibiting intestinal macrophage polarization (2022-08-04)
作者：Shuiliang Ruan:1.Department of Gastroenterology, the Second Affiliated Hospital of Jiaxing University, Jiaxing, China. ; Li Zha:1.Department of Gastroenterology, the Second Affiliated Hospital of Jiaxing University, Jiaxing, China. ;
期刊：Moronic acid improves intestinal inflammation in mice with chronic colitis by inhibiting intestinal macrophage polarization
影响因子 ：3.6
引用产品： 小鼠肠巨噬细胞
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NOX4 promotes mucosal barrier injury in inflammatory bowel disease by mediating macrophages M1 polarization through ROS (2012-01-20)
作者：Chenyang Han:1.Department of Pharmacy, The Second Affiliated Hospital of Jiaxing University, 314001, China. Electronic address: 691513770@qq.com. ; Yongjia Sheng:1.Department of Pharmacy, The Second Affiliated Hospital of Jiaxing University, 314001, China. Electronic address: 56975264@qq.com. ; Jin Wang:1.Department of Pharmacy, The Second Affiliated Hospital of Jiaxing University, 314001, China. Electronic address: wt198805@126.com. ; Xiaohong Zhou:1.Department of Pharmacy, The Second Affiliated Hospital of Jiaxing University, 314001, China. Electronic address: 13736496736@163.com. ; Wenyan Li:1.Department of Pharmacy, The Second Affiliated Hospital of Jiaxing University, 314001, China. Electronic address: 85648857@qq.com. ; Caiqun Zhang:1.Department of Pharmacy, The Second Affiliated Hospital of Jiaxing University, 314001, China. Electronic address: caiqun_zhang@126.com. ; Li Guo:1.Department of Center Laboratory, The Second Affiliated Hospital of Jiaxing University, China. Ele
期刊：NOX4 promotes mucosal barrier injury in inflammatory bowel disease by mediating macrophages M1 polarization through ROS
DOI：10.1016/j.intimp.2021.108361
影响因子 ：5.6
引用产品： 小鼠肠巨噬细胞
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