产品中心Cell Resources
联系我们CONTACT US
400-999-210024小时服务热线
人外周血单核细胞
Human Peripheral Blood Monocyte Cells
货号：CP-H182
规格：5×10⁵Cells/T25培养瓶
价格： ￥5880
培养基货号： CM-H182
产品概述
人外周血单核细胞
Cat NO.: CP-H182
-
产品名称：人外周血单核细胞
-
组织来源：外周血
-
产品规格：5×10⁵Cells/T25培养瓶
-
细胞简介：
人外周血单核细胞分离自外周血；外周血是除骨髓之外的血液，临床上常用一些方法把骨髓中的造血干细胞释放到血液中，再在从血液中提取分离得到造血干细胞，我们把这样得到的干细胞称为外周血干细胞，在二十一世纪初人类开始的生命方舟计划中首次提出外周血这一新概念。单核细胞起源于骨髓中的造血干细胞，并在骨髓中发育。当它们从骨髓进入血液时仍然是尚未成熟的细胞。与其他血细胞比较，单核细胞内含有更多的非特异性脂酶，并且具有更强的吞噬作用。单核细胞在血液中停留2-3天后迁移到周围组织中，细胞体积继续增大，直径可达50-80μm，细胞内所含的溶酶体颗粒和线粒体的数目也增多，成为成熟的细胞。固定在组织中的单核细胞称为组织巨噬细胞，它们经常大量存在于淋巴结、肺泡壁、骨髓、肝和脾等器官。激活了的单核细胞和组织巨噬细胞能生成并释放多种细胞毒、干扰素和白细胞介素，参与机体防卫机制，还产生一些能促进内皮细胞和平滑肌细胞生长的因子。在炎症周围单核细胞能进行细胞分裂，并包围异物。
-
方法简介：
普诺赛实验室分离的人外周血单核细胞采用密度梯度离心法结合差速贴壁法制备而来，细胞总量约为5×10⁵cells/瓶。
-
质量检测：
普诺赛实验室分离的人外周血单核细胞经CD14免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
-
培养信息：
包被条件 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-H182 换液频率 每2-3天换液一次 生长特性 半贴壁半悬浮 细胞形态 圆形、巨噬细胞样 传代特性 不增殖；不传代 消化液 0.25%胰蛋白酶 人外周血单核细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
-
HNRNPC suppresses tumor immune microenvironment by activating Treg cells promoting the progression of prostate cancer (2023-02-13)
作者：Yifei Cheng:1.Department of Urologic Surgery, Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research & Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, China. ; 2.Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China. ; Lu Li:1.State Key Laboratory of Translational Medicine and Innovative Drug Development, Jiangsu Simcere Diagnostics Co., Ltd., Nanjing, China. ; Xiyi Wei:1.Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China. ; 2.The State Key Lab of Reproductive, Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China. ; Fan Xu:1.Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research & Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, China. ; Xiaochen Huang:1.Department of Pathology, Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research & Affiliated Cancer Hospital of Nanjing Medical Unive
期刊：HNRNPC suppresses tumor immune microenvironment by activating Treg cells promoting the progression of prostate cancer
影响因子 ：5.7
引用产品： 人外周血单核细胞
-
Th1 promotes M1 polarization of intestinal macrophages to regulate colitis-related mucosal barrier damage (2023-04-04)
作者：Shuiliang Ruan:1.The Second Affiliated Hospital of Jiaxing University, Jiaxing 314001, Zhejiang, China. ; Liang Xu:1.Jiaxing University Master Degree Cultivation Base, Zhejiang Chinese Medical University, Hangzhou 310000, Zhejiang, China. ; Yongjia Sheng:1.The Second Affiliated Hospital of Jiaxing University, Jiaxing 314001, Zhejiang, China. ; Jin Wang:1.The Second Affiliated Hospital of Jiaxing University, Jiaxing 314001, Zhejiang, China. ; Xiaohong Zhou:1.The Second Affiliated Hospital of Jiaxing University, Jiaxing 314001, Zhejiang, China. ; Caiqun Zhang:1.The Second Affiliated Hospital of Jiaxing University, Jiaxing 314001, Zhejiang, China. ; Li Guo:1.The Second Affiliated Hospital of Jiaxing University, Jiaxing 314001, Zhejiang, China. ; Wenyan Li:1.The Second Affiliated Hospital of Jiaxing University, Jiaxing 314001, Zhejiang, China. ; Chenyang Han:1.The Second Affiliated Hospital of Jiaxing University, Jiaxing 314001, Zhejiang, China. ;
期刊：Th1 promotes M1 polarization of intestinal macrophages to regulate colitis-related mucosal barrier damage
影响因子 ：5.2
-
Ester tethered artemisinin-isatin hybrids: design, synthesis and anti-leukemic activity evaluation (2023-08-21)
作者：Peng Wang: 1.The First Clinical College, Shandong University of Traditional Chinese Medicine, **an, China;2.Department of Critical Care Unit, Shandong Provincial Hospital Affiliated to Shandong First Medical University, **an, China; Weiwei Wang: 1.The First Clinical College, Shandong University of Traditional Chinese Medicine, **an, China; Lei Zhou: 1.The First Clinical College, Shandong University of Traditional Chinese Medicine, **an, China; Lin Wang: 1.The First Clinical College, Shandong University of Traditional Chinese Medicine, **an, China; Yang Zhao: 1.The First Clinical College, Shandong University of Traditional Chinese Medicine, **an, China; Jiarui Xu: 1.Department of Critical Care Unit, Shandong Provincial Hospital Affiliated to Shandong First Medical University, **an, China; Dai Yuan: 1.Department of Hematology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, **an, China; Li Kong: 1.Department of Emergency Center, Shandong University of Tradit
期刊：Ester tethered artemisinin-isatin hybrids: design, synthesis and anti-leukemic activity evaluation
DOI：10.1007/s00044-023-03122-x
影响因子 ：2.6
引用产品： K-562 [K562] 细胞 , K562/ADR 细胞 , CCRF-CEM [CCRF CEM] 细胞 , 人外周血单核细胞
FAQs
Q：{{item.question}}
A：
产品资料
识别码示意图
