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产品概述
小鼠软骨细胞
Cat NO.: CP-M087
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产品名称：小鼠软骨细胞
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组织来源：软骨组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
小鼠软骨细胞分离自软骨组织；软骨组织由软骨细胞、基质及纤维构成。软骨组织再生能力强，这些增生的幼稚细胞形似纤维母细胞，以后逐渐变为软骨母细胞，并形成软骨基质，细胞被埋在软骨陷窝内而变为静止的软骨细胞。根据软骨组织内所含纤维成分的不同，可将软骨分为透明软骨、弹性软骨和纤维软骨三种，其中以透明软骨的分布较广，结构也较典型。软骨细胞(chondrocyte)位于软骨陷窝内。幼稚的软骨细胞位于软骨组织的表层，单个分布，体积较小，呈椭圆形，长轴与软骨表面平行，越向深层的软骨细胞体积之间增大呈圆形，细胞核圆形或卵圆形，染色浅，细胞质弱嗜碱性，常见数量不一的脂滴。成熟的软骨细胞多2-8个成群分布于软骨陷窝内，这些软骨细胞由同一个母细胞分裂增殖而成，称为同源细胞群。电镜下，软骨细胞有突起和皱褶，细胞质内有大量的粗面内质网和发达的高尔基复合体及少量的线粒体。在组织切片中，软骨细胞收缩为不规则形，在软骨囊和细胞之间出现较大的腔隙。体外培养的软骨细胞对于研究其生理功能、药物作用以及各种致病因素作用下的病理生理改变具重要意义。
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方法简介：
普诺赛实验室分离的小鼠软骨细胞采用胶原酶 - 中性蛋白酶联合消化制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的小鼠软骨细胞经Ⅱ型胶原蛋白免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-M087 换液频率 每3-4天换液一次 生长特性 贴壁 细胞形态 梭形、多角形 传代特性 可传2-3代左右 消化液 0.25%胰蛋白酶 小鼠软骨细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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A novel extracellular vesicles production system harnessing matrix homeostasis and macrophage reprogramming mitigates osteoarthritis (2024-02-28)
作者：Tianqi Wang:1.Departments of Orthopaedic Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117597, Singapore. ; 2.Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. ; Hongqi Zhao:1.Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. ; Yi Zhang:1.School of Public Health, Southwest Medical University, Luzhou, 646000, Sichuan, China. ; Yanshi Liu:1.Department of Orthopaedics, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, 646000, China. ; Jialin Liu:1.Department of Oral Implantology, The Affiliated Stomatology Hospital of Southwest Medical University, Luzhou, Sichuan, 64600, China. ; Ge Chen:1.Department of Orthopaedics, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, 646000, China. ; Ke Duan:1.Sichuan Provincial Laboratory of
期刊：A novel extracellular vesicles production system harnessing matrix homeostasis and macrophage reprogramming mitigates osteoarthritis
DOI：10.1186/s12951-024-02324-8
影响因子 ：10.2
引用产品： 小鼠软骨细胞 , RPMI-1640 培养基
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Early diagnosis of heterotopic ossification with a NIR fluorescent probe by targeting type II collagen (2022-12-14)
作者：Zheng Wang:1.Department of Orthopedics Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan 430071, China. lqy-032529@163.com. ; 2.Hubei Clinical Medical Research Center of Trauma and Microsurgery, Wuhan, Hubei 430071, China. ; Xinzeyu Yi:1.Department of Orthopedics Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan 430071, China. lqy-032529@163.com. ; 2.Hubei Clinical Medical Research Center of Trauma and Microsurgery, Wuhan, Hubei 430071, China. ; Wanrong Yi:1.Department of Orthopedics Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan 430071, China. lqy-032529@163.com. ; 2.Hubei Clinical Medical Research Center of Trauma and Microsurgery, Wuhan, Hubei 430071, China. ; Chao Jian:1.Department of Orthopedics Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan 430071, China. lqy-032529@163.com. ; 2.Hubei Clinical Medical Research Center of Trauma and Microsurgery, Wuhan, Hubei 430071, China. ; Baiwen Qi:1.Depar
期刊：Early diagnosis of heterotopic ossification with a NIR fluorescent probe by targeting type II collagen
影响因子 ：7.6
引用产品： MPC 细胞 , 小鼠软骨细胞 , DMEM高糖 培养基
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Artesunate ameliorates osteoarthritis cartilage damage by updating MTA1 expression and promoting the transcriptional activation of LXA4 to suppress the JAK2/STAT3 signaling pathway (2022-11-26)
作者：Chengjin Zhao:1.Department of Bone and Joint Surgery, Xi'an Jiaotong University Second Affiliated Hospital, Xi'an 710004, Shaanxi, China. ; 2.Yan'an University Affiliated Hospital, Yan'an 716000, Shaanxi, China. ; Li Zhao:1.Yan'an University Affiliated Hospital, Yan'an 716000, Shaanxi, China. ; Yuhu Zhou:1.Yan'an University Affiliated Hospital, Yan'an 716000, Shaanxi, China. ; Yangyang Feng:1.Yan'an University Affiliated Hospital, Yan'an 716000, Shaanxi, China. ; Nannan Li:1.Yan'an University Affiliated Hospital, Yan'an 716000, Shaanxi, China. ; Kunzheng Wang:1.Department of Bone and Joint Surgery, Xi'an Jiaotong University Second Affiliated Hospital, Xi'an 710004, Shaanxi, China. ;
期刊：Artesunate ameliorates osteoarthritis cartilage damage by updating MTA1 expression and promoting the transcriptional activation of LXA4 to suppress the JAK2/STAT3 signaling pathway
影响因子 ：5.1
引用产品： 小鼠软骨细胞 , 小鼠软骨细胞完全培养基
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Glaucocalyxin B inhibits cartilage inflammatory injury in rheumatoid arthritis by regulating M1 polarization of synovial macrophages through NF-κB pathway (2009-01-20)
作者：Chenyang Han:1.Department of Pharmacy, The Second Affiliated Hospital of Jiaxing University, Zhejiang, China. ; Yi Yang:1.Department of Pharmacy, The Second Affiliated Hospital of Jiaxing University, Zhejiang, China. ; Yongjia Sheng:1.Department of Pharmacy, The Second Affiliated Hospital of Jiaxing University, Zhejiang, China. ; Jin Wang:1.Department of Pharmacy, The Second Affiliated Hospital of Jiaxing University, Zhejiang, China. ; Xiaohong Zhou:1.Department of Center Laboratory, The Second Affiliated Hospital of Jiaxing University, Zhejiang, China. ; Wenyan Li:1.Department of Pharmacy, The Second Affiliated Hospital of Jiaxing University, Zhejiang, China. ; Li Guo:1.Department of Center Laboratory, The Second Affiliated Hospital of Jiaxing University, Zhejiang, China. ; Caiqun Zhang:1.Department of Pharmacy, The Second Affiliated Hospital of Jiaxing University, Zhejiang, China. ; Qiao Ye:1.Department of Rheumatology and Immunology, The Second Affiliated Hospital of Jiaxing
期刊：Glaucocalyxin B inhibits cartilage inflammatory injury in rheumatoid arthritis by regulating M1 polarization of synovial macrophages through NF-κB pathway
影响因子 ：5.2
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The inhibitory roles of Ihh downregulation on chondrocyte growth and differentiation (2011-07-20)
作者：Ang Deng:1.Department of Spine Surgery, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China. ; Hongqi Zhang:1.Department of Spine Surgery, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China. ; Minyu Hu:1.Department of Nutrition and Food Hygiene, School of Public Health, Central South University, Changsha, Hunan 410078, P.R. China. ; Shaohua Liu:1.Department of Spine Surgery, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China. ; Yuxiang Wang:1.Department of Spine Surgery, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China. ; Qile Gao:1.Department of Spine Surgery, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China. ; Chaofeng Guo:1.Department of Spine Surgery, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China. ;
期刊：The inhibitory roles of Ihh downregulation on chondrocyte growth and differentiation
影响因子 ：2.7
引用产品： 小鼠软骨细胞
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