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产品概述
产品信息
| 别名 | Catabolin, Lymphocyte-Activating Factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endoge_x0002_nous Mediator (LEM), Mononuclear Cell Factor (MCF) |
| 物种 | Human |
| 表达宿主 | E.coli |
| 序列信息 | MASAPVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS with polyhistidine tag at the C-terminus. |
| 检索号 | P01584.2 |
| 分子量 | 18.48 kDa |
| 表观分子量 | 17 kDa |
| 标签 | His-tag at the C-terminus |
| 生物活性 | Measure by its ability to induce proliferation in D10.G4.1 cells. The ED50 for this effect is <10 pg/mL. The specific activity of recombinant human IL-1 beta is approximately >1.5 x 10^8 IU/mg.Measure by its ability to induce IL-8 secretion in HT29 cells. The ED50 for this effect is 1.8-5.1 ng/mL. |
产品特性
| 纯度 | >98% as determined by SDS-PAGE. Ni-NTA chromatography |
| 内毒素 | <0.1 EU per 1 μg of the protein by the LAL method. |
| 保存 | Lyophilized protein should be stored at -5~-20°C for 1 year. Upon reconstitution, store at 2-8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g.0.1% BSA,10%FBS,5%HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20°C or -80°C for 3-6 months. |
| 运输 | Ambient temperature or ice pack. |
| 制剂 | The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0. |
| 复融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL.Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
背景介绍
参考文献
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Formononetin Inhibits Microglial Inflammatory Response and Contributes to Spinal Cord Injury Repair by Targeting the EGFR/MAPK Pathway (2023-03-28)
作者：Haiping Fu:1.Department of Spine Surgery, Inner Mongolia People's Hospital, Hohhot, Inner Mongolia, China. ; Mingdong Li:1.Department of Orthopedics and Traumatology, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou, Hainan China. ; Yanqiang Huan:1.Department of Spine Surgery, Inner Mongolia People's Hospital, Hohhot, Inner Mongolia, China. ; Xiaolei Wang:1.Department of Spine Surgery, Inner Mongolia People's Hospital, Hohhot, Inner Mongolia, China. ; Mingkai Tao:1.Department of Spine Surgery, Inner Mongolia People's Hospital, Hohhot, Inner Mongolia, China. ; Tianqi Jiang:1.Department of Spine Surgery, Inner Mongolia People's Hospital, Hohhot, Inner Mongolia, China. ; Hongbin Xie:1.Department of Spine Surgery, Inner Mongolia People's Hospital, Hohhot, Inner Mongolia, China. ; Wujisiguleng:1.Department of Spine Surgery, Inner Mongolia People's Hospital, Hohhot, Inner Mongolia, China. ; Gegentana:1.Department of International Medical Center
期刊：Formononetin Inhibits Microglial Inflammatory Response and Contributes to Spinal Cord Injury Repair by Targeting the EGFR/MAPK Pathway
DOI：10.1080/08820139.2023.2183135
影响因子 ：2.8
引用产品： IL-1β/IL1F2, Human, Recombinant , IL-1β/IL1F2, Mouse, Recombinant
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Overexpression of long non-coding RNA XIST promotes IL-1β-induced degeneration of nucleus pulposus cells through targeting miR-499a-5p (2003-01-20)
作者：Jun He:1.Department of Orthopedics, Zhejiang Hospital, Xihu District, Hangzhou City, Zhejiang Province, 310030, China. ; Jing Yang:1.Department of Cardiology, Zhejiang Hospital, Xihu District, Hangzhou, Zhejiang, 310013, China. ; Tulan Shen:1.Outpatient Department, Zhejiang Hospital, Xihu District, Hangzhou City, Zhejiang Province, 310030, China. ; Jian He:1.Department of Orthopedics, Zhejiang Hospital, Xihu District, Hangzhou City, Zhejiang Province, 310030, China. Electronic address: hejian_jhe@163.com. ;
期刊：Overexpression of long non-coding RNA XIST promotes IL-1β-induced degeneration of nucleus pulposus cells through targeting miR-499a-5p
影响因子 ：3.3
引用产品： DMEM/F12 培养基 , 青霉素-链霉素溶液 (双抗)，100× , 特级胎牛血清 , IL-1β/IL1F2, Human, Recombinant
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