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产品概述
产品信息
别名 | Type II interferon, T-cell interferon, MAF |
物种 | Human |
表达宿主 | E.coli |
序列信息 | MQDPYVKEAENLKKYFNAGHSDVADNGTLFLGILKNWKEESDRKIMQSQIVSFYFKLFKNFKDDQSIQKSVETIKEDMNVKFFNSNKKKRDDFEKLTNYSVTDLNVQRKAIHELIQVMAELSPAAKTGKRKRSQMLFQGRRASQ with polyhistidine tag at the C-terminus. |
检索号 | P01579.1 |
分子量 | 17.7 kDa |
表观分子量 | 17 kDa |
标签 | His-tag at the C-terminus |
生物活性 | Measure by its ability to induce cytotoxicity in HT29 cells.The ED50 for this effect is <0.5 ng/mL.The specific activity of recombinant human IFN gamma is approximately >1.3 x 10^7 IU/mg. |
产品特性
纯度 | >95% as determined by SDS-PAGE. Ni-NTA chromatography. |
内毒素 | <0.01 EU per 1 μg of the protein by the LAL method. |
保存 | Lyophilized protein should be stored at -5~-20°C for 1 year. Upon reconstitution, store at 2-8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g.0.1% BSA,10%FBS,5%HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20°C or -80°C for 3-6 months. |
运输 | Ambient temperature or ice pack. |
制剂 | The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0. |
复融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL.Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
背景介绍
参考文献
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Screening potential diagnostic biomarkers for PLA2R‑associated idiopathic membranous nephropathy by WGCNA analysis and LASSO algorithm (2025-01-13)
期刊:RENAL FAILURE
DOI:10.1080/0886022X.2024.2438859
影响因子 :3.0
引用产品: 人肾足突细胞 , RPMI-1640 培养基 , Interferon γ/IFN-γ/IFNG, Human, Recombinant
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miR-664a-5p promotes experimental membranous nephropathy progression through HIPK2/Calpain1/GSα-mediated autophagy inhibition (2024-01-07)
期刊:JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
影响因子 :4.3
引用产品: HK-2 细胞 , 人肾足突细胞 , 293T [HEK-293T] 细胞 , MEM(含NEAA) 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液(双抗),100× , RPMI-1640 培养基 , Interferon γ/IFN-γ/IFNG, Human, Recombinant , DMEM高糖 培养基
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