产品中心Cell Resources
联系我们CONTACT US
400-999-210024小时服务热线
产品概述
大鼠肌腱成纤维细胞
Cat NO.: CP-R237
-
产品名称：大鼠肌腱成纤维细胞
-
组织来源：肌腱组织
-
产品规格：5×10⁵Cells/T25培养瓶
-
细胞简介：
大鼠肌腱成纤维细胞分离自肌腱组织；肌腱是肌腹两端的索状或膜状致密结缔组织，便于肌肉附着和固定。一块肌肉的肌腱分附在两块或两块以上的不同骨上，是由于肌腱的牵引作用才能使肌肉的收缩带动不同骨的运动。每一块骨骼肌都分成肌腹和肌腱两部分，肌腹由肌纤维构成，色红质软，有收缩能力，肌腱由致密结缔组织构成，色乳白较硬，没有收缩能力。肌腱把骨骼肌附着于骨骼。长肌的肌腱多呈圆索状，阔肌的肌腱阔而薄，呈膜状，又叫腱膜。此处的肌腹即为通常所说的红肌，而肌腱即为白肌，分别控制肌肉的力量、爆发力和耐力。成纤维细胞是疏松结缔组织的主要细胞成分，由胚胎时期的间充质细胞分化而来；成纤维细胞较大，轮廓清楚，多为突起的纺锤形或星形的扁平状结构，其细胞核呈规则的卵圆形，核仁大而明显。成纤维细胞功能活动旺盛，细胞质嗜弱碱性，具明显的蛋白质合成和分泌活动，在一定条件下，它可以实现跟纤维细胞的互相转化；成纤维细胞对不同程度的细胞变性、坏死和组织缺损的修复有着十分重要的作用。刚分离的肺成纤维细胞呈圆形、折光性良好，悬浮于培养基中。30min细胞贴壁，其中部分开始伸出伪足，表现为小的突起；6h后细胞基本贴壁完全，伸展成梭形，胞核清晰，分布较均匀，散在生长，不聚集成团；细胞生长迅速，5-7天即呈融合状态，细胞排列紧密，有的交叉重叠生长，平坦、胞体较大，细胞质透明，细胞核较大，呈椭圆形，颜色淡。细胞融合，并彼此连接成网状；细胞呈突起的纺锤形或星形的扁平分布。
-
方法简介：
普诺赛实验室分离的大鼠肌腱成纤维细胞采用混合胶原酶消化法结合差速贴壁法制备而来，细胞总量约为5×10⁵cells/瓶。
-
质量检测：
普诺赛实验室分离的大鼠肌腱成纤维细胞经Vimentin免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
-
培养信息：
包被条件 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-R237 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 成纤维细胞样 传代特性 可传5代左右；3代以内状态最佳 消化液 0.25%胰蛋白酶 大鼠肌腱成纤维细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
-
A Polyvinyl Alcohol/Acrylamide Hydrogel with Enhanced Mechanical Properties Promotes Full-Thickness Skin Defect Healing by Regulating Immunomodulation and Angiogenesis Through Paracrine Secretion (2024-03-06)
作者：Peng Wang: 1.State Key Laboratory of Pharmaceutical Biotechnology, Division of Sports Medicine and Adult Reconstructive Surgery, Department of Orthopedic Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing University, Nanjing 210008, China;2.Jiangsu Engineering Research Center for 3D Bioprinting, Nanjing 210008, China;3.Institute of Medical 3D Printing, Nanjing University, Nanjing 210093, China; Liping Qian: 1.Centre for Experimental Animals, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing University, Nanjing 210008, China; Huixin Liang: 1.State Key Laboratory of Pharmaceutical Biotechnology, Division of Sports Medicine and Adult Reconstructive Surgery, Department of Orthopedic Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing University, Nanjing 210008, China;2.Jiangsu Engineering Research Center for 3D Bioprinting, Nanjin
期刊：A Polyvinyl Alcohol/Acrylamide Hydrogel with Enhanced Mechanical Properties Promotes Full-Thickness Skin Defect Healing by Regulating Immunomodulation and Angiogenesis Through Paracrine Secretion
影响因子 ：12.8
引用产品： 大鼠肌腱成纤维细胞
-
Poly-l-lactide-co-ε-caprolactone (PLCL) and poly-l-lactic acid (PLLA)/gelatin electrospun subacromial spacer improves extracellular matrix (ECM) deposition for the potential treatment of irreparable rotator cuff tears (2023-06-21)
作者：Chang Liu:1.Department of Joint and Trauma Surgery, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China; Department of Orthopaedic Surgery, The Lingnan Hospital of Sun Yat-sen University, Guangzhou 510630, China. ; Shihai Jiang:1.Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University Hospital Leipzig, Leipzig 04103, Germany. ; Wenbin Xu:1.Department of Orthopaedic Surgery, The Lingnan Hospital of Sun Yat-sen University, Guangzhou 510630, China. ; Xi Chen:1.Department of Joint and Trauma Surgery, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China. ; Ronghan He:1.Department of Joint and Trauma Surgery, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China. ; Kunxue Deng:1.Medprin Regenerative Medical Technologies Co., Ltd, Guangzhou 510630, China. ; Hengxian Su:1.Zhujiang Hospital of Southern Medical University, Guangzhou 510282, China. ; XiaoPeng Yin:1.Department of Joint
期刊：Poly-l-lactide-co-ε-caprolactone (PLCL) and poly-l-lactic acid (PLLA)/gelatin electrospun subacromial spacer improves extracellular matrix (ECM) deposition for the potential treatment of irreparable rotator cuff tears
DOI：10.1016/j.ijbiomac.2023.125522
影响因子 ：8.2
-
An engineered tenogenic patch for the treatment of rotator cuff tear (2022-11-21)
作者：Shaoshen Zhu: 1.Department of Joint and Trauma Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China; Jianfeng Hou: 1.Department of Joint and Trauma Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China; Chang Liu: 1.Department of Joint and Trauma Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China; Peng Liu: 1.College of Materials Science and Engineering, Hunan University, Changsha 410082, China; Ting Guo: 1.College of Materials Science and Engineering, Hunan University, Changsha 410082, China; Zhengjie Lin: 1.Department of Joint and Trauma Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China; Xianwei Wang: 1.Department of Vascular Surgery, Department of Orthopaedic Surgery, Xiangya Hospital, Central South University, Changsha 410008, China; Chunmiao Wu: 1.College of Life Science and Technology, Jinan University, Guangzhou, China; Dichun Hu
期刊：An engineered tenogenic patch for the treatment of rotator cuff tear
DOI：10.1016/j.matdes.2022.111402
影响因子 ：9.4
引用产品： 大鼠肌腱成纤维细胞
-
The Regenerative Role of Gelatin in PLLA Electrospun Membranes for the Treatment of Chronic Massive Rotator Cuff Injuries (2010-01-20)
作者：Chang Liu:1.Department of Joint and Trauma Surgery, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510630, China. ; 2.East China Institute of Digital Medical Engineering, Shangrao, 334000, China. ; Shihai Jiang:1.Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University Hospital Leipzig, Leipzig, 04103, Germany. ; Yu Wu:1.Department of Plastic and Aesthetic Surgery, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510630, China. ; 2.East China Institute of Digital Medical Engineering, Shangrao, 334000, China. ; Libiao Liu:1.East China Institute of Digital Medical Engineering, Shangrao, 334000, China. ; 2.Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, Department of Mechanical Engineering, Key Laboratory for Advanced Materials Processing Technology, Ministry of Education, Department of Mechanical Engineering, Tsinghua University, Beijing, 100084, China. ; Shouwen Su:1.Department of Joint and Tr
期刊：The Regenerative Role of Gelatin in PLLA Electrospun Membranes for the Treatment of Chronic Massive Rotator Cuff Injuries
影响因子 ：5.9
引用产品： 大鼠肌腱成纤维细胞 , PUMC-HUVEC-T1
FAQs
Q：{{item.question}}
A：
产品资料
识别码示意图
