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人Ⅱ型肺泡上皮细胞
Human Alveolar Type II Epithelial Cells
货号：CP-H209
规格：5×10⁵Cells/T25培养瓶
价格： ￥7880
培养基货号： CM-H209
产品概述
人Ⅱ型肺泡上皮细胞
Cat NO.: CP-H209
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产品名称：人Ⅱ型肺泡上皮细胞
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组织来源：肺组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
人Ⅱ型肺泡上皮细胞分离自肺组织；肺泡由单层上皮细胞构成的半球状囊泡。肺中的支气管经多次反复分枝成无数细支气管，它们的末端膨大成囊，囊的四周有很多突出的小囊泡，即为肺泡。小肺泡细胞，又称I型肺泡细胞，厚约0.1微米，基底部是基底膜，无增殖能力。大肺泡细胞，又称Ⅱ型肺泡细胞，分泌表面活性物质(二棕榈酰卵磷脂)，以降低肺泡表面张力。Ⅱ型肺泡细胞位于Ⅰ型肺泡细胞之间，数量较Ⅰ型肺泡细胞多，但覆盖面积比Ⅰ型肺泡细胞小。细胞立方形或圆形,顶端突入肺泡腔。细胞核圆形，胞质着色浅、呈泡沫状。电镜下，细胞游离而有少量微绒毛，胞质内富含线粒体和溶酶体，有较发达的粗面内质网和高尔基复合体。核上方有较多的分泌颗粒，电子密度高、大小不等，直径约0.1-1.0μm颗粒内含有平行排列的板层状结构，称为嗜饿性板层小体。小体内的主要成分为磷脂，以二棕榈酰卵磷脂为主，此外还有糖胺多糖及蛋白质等。颗粒内物质释放出来后，在肺泡表面形成一层粘液层，称为表面活性物质(surfactant)。表面活性物质有降低肺泡表面张力、稳定肺泡大小的作用。呼气时肺泡缩小，表面活性物质密度增加，表面张力降低，防止肺泡过度塌陷；吸气时肺泡扩张，表面活性物质密度减小，肺泡回缩力加大，可防止肺泡过度膨胀。表面活性物质的缺乏或变性均可引起肺不张，过度通气可造成表面活性物质缺乏；吸入毒气可直接破坏表面活性物质。Ⅱ型肺泡细胞有分裂、增殖并分化为Ⅰ型肺泡细胞的潜能，故具有修复受损伤上皮的作用。
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方法简介：
普诺赛实验室分离的人Ⅱ型肺泡上皮细胞采用弹性蛋白酶消化法制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的人Ⅱ型肺泡上皮细胞经SP-C免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 鼠尾胶原Ⅰ(2-5μg/cm2) 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-H209 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 上皮细胞样 传代特性 可传1-2代左右 消化液 0.25%胰蛋白酶 人Ⅱ型肺泡上皮细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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LncRNA HOTTIP promotes LPS-induced lung epithelial cell injury by recruiting DNMT1 to epigenetically regulate SP-C (2024-02-27)
作者：Shuang Li:1.Department of Emergency The First Affiliated Hospital of Jiamusi University Jiamusi China. ; Shuangjia Li:1.Department of Emergency The First Affiliated Hospital of Jiamusi University Jiamusi China. ; Zhanqun Gao:1.Department of Emergency The First Affiliated Hospital of Jiamusi University Jiamusi China. ; Yang Liu:1.Department of Emergency The First Affiliated Hospital of Jiamusi University Jiamusi China. ;
期刊：LncRNA HOTTIP promotes LPS-induced lung epithelial cell injury by recruiting DNMT1 to epigenetically regulate SP-C
影响因子 ：4.1
引用产品： 人Ⅱ型肺泡上皮细胞
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Smoke and Spike: Benzo[a]pyrene Enhances SARS-CoV-2 Infection by Boosting NR4A2-Induced ACE2 and TMPRSS2 Expression (2023-07-10)
作者：Wenbin Liu:1.Key Laboratory of Biosafety Defense, Ministry of Education, Shanghai, 200433, China. ; 2.Shanghai Key Laboratory of Medical Biodefense, Shanghai, 200433, China. ; 3.Department of Epidemiology, Second Military Medical University, Shanghai, 200433, China. ; Yue Zhao:1.Key Laboratory of Biosafety Defense, Ministry of Education, Shanghai, 200433, China. ; 2.Shanghai Key Laboratory of Medical Biodefense, Shanghai, 200433, China. ; 3.Department of Epidemiology, Second Military Medical University, Shanghai, 200433, China. ; Junyan Fan:1.Key Laboratory of Biosafety Defense, Ministry of Education, Shanghai, 200433, China. ; 2.Shanghai Key Laboratory of Medical Biodefense, Shanghai, 200433, China. ; 3.Department of Epidemiology, Second Military Medical University, Shanghai, 200433, China. ; Jiaying Shen:1.Shanghai Key Laboratory of Medical Biodefense, Shanghai, 200433, China. ; 2.Department of Epidemiology, Second Military Medical University, Shanghai, 200433, China. ; 3.Shanghai
期刊：Smoke and Spike: Benzo[a]pyrene Enhances SARS-CoV-2 Infection by Boosting NR4A2-Induced ACE2 and TMPRSS2 Expression
影响因子 ：15.1
引用产品： 人Ⅱ型肺泡上皮细胞
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The RNA-Splicing Ligase RTCB Promotes Influenza A Virus Replication by Suppressing Innate Immunity via Interaction with RNA Helicase DDX1 (2023-08-09)
作者：Lei Cao:1.State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China. ; 2.College of Animal Medicine, Huazhong Agricultural University, Wuhan, China. ; Xianfeng Hui:1.State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China. ; 2.College of Animal Medicine, Huazhong Agricultural University, Wuhan, China. ; Ting Xu:1.State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China. ; 2.College of Animal Medicine, Huazhong Agricultural University, Wuhan, China. ; Haiying Mao:1.State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China. ; 2.College of Animal Medicine, Huazhong Agricultural University, Wuhan, China. ; Xian Lin:1.State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China. ; 2.College of Animal Medicine, Huazhong Agricultural University, Wuhan, China. ; Kun Huang:1.State Key Laborato
期刊：The RNA-Splicing Ligase RTCB Promotes Influenza A Virus Replication by Suppressing Innate Immunity via Interaction with RNA Helicase DDX1
影响因子 ：4.4
引用产品： 人Ⅱ型肺泡上皮细胞 , 人Ⅱ型肺泡上皮细胞完全培养基
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CircTDRD9 contributes to sepsis-induced acute lung injury by enhancing the expression of RAB10 via directly binding to miR-223-3p (2023-08-07)
作者：Rui Zhang:1.Emergency Department, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi Province, China. ; Xiaoyan Dang: Jie Liu: Hui Feng: Jiangli Sun: Zhuo Peng:
期刊：CircTDRD9 contributes to sepsis-induced acute lung injury by enhancing the expression of RAB10 via directly binding to miR-223-3p
DOI：10.1097/SHK.0000000000002169
影响因子 ：3.1
引用产品： A549 [A-549] 细胞 , 人Ⅱ型肺泡上皮细胞
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C/EBP homologous protein promotes Sonic Hedgehog secretion from type II alveolar epithelial cells and activates Hedgehog signaling pathway of fibroblast in pulmonary fibrosis (2004-02-20)
作者：Xiaoyu Yang:1.Department of Respiratory and Critical Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 1 Shuai Fu Yuan Street, Dong Cheng District, Beijing, 100730, China. ; Wei Sun:1.Department of Respiratory and Critical Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 1 Shuai Fu Yuan Street, Dong Cheng District, Beijing, 100730, China. ; 2.Medical Research Center, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 1 Shuai Fu Yuan Street, Dong Cheng District, Beijing, China. ; Xiaoyan Jing:1.Department of Respiratory and Critical Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 1 Shuai Fu Yuan Street, Dong Cheng District, Beijing, 100730, China. ; Qian Zhang:1.Department of Respiratory and Critical Medi
期刊：C/EBP homologous protein promotes Sonic Hedgehog secretion from type II alveolar epithelial cells and activates Hedgehog signaling pathway of fibroblast in pulmonary fibrosis
DOI：10.1186/s12931-022-02012-x
影响因子 ：5.8
引用产品： 人Ⅱ型肺泡上皮细胞
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CREB1 Transcriptionally Activates LTBR to Promote the NF-κB Pathway and Apoptosis in Lung Epithelial Cells. (2022-09-09)
作者：Zhengyun Hu:1.Department of Pediatrics, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China. ; 2.Department of Pediatrics, Shanghai Songjiang District Central Hospital, Shanghai 201600, China. ; Guoping Zhou:1.Department of Pediatrics, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China. ;
期刊：CREB1 Transcriptionally Activates LTBR to Promote the NF-κB Pathway and Apoptosis in Lung Epithelial Cells.
影响因子 ：2.8
引用产品： 人Ⅱ型肺泡上皮细胞 , 293T [HEK-293T] 细胞
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LncRNA-ATB regulates epithelial-mesenchymal transition progression in pulmonary fibrosis via sponging miR-29b-2-5p and miR-34c-3p (2006-01-20)
作者：Qi Xu:1.Key Laboratory of Modern Toxicology of Ministry of Education, Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing, China. ; Demin Cheng:1.Key Laboratory of Modern Toxicology of Ministry of Education, Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing, China. ; Yi Liu:1.Key Laboratory of Modern Toxicology of Ministry of Education, Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing, China. ; Honghong Pan:1.Key Laboratory of Modern Toxicology of Ministry of Education, Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing, China. ; Guanru Li:1.Key Laboratory of Modern Toxicology of Ministry of Education, Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing, China. ; Ping Li:1.Key Laboratory of Modern Toxicology of Ministry of Education, Center for Global Health, School of Public Health, Nanjing Medical Uni
期刊：LncRNA-ATB regulates epithelial-mesenchymal transition progression in pulmonary fibrosis via sponging miR-29b-2-5p and miR-34c-3p
影响因子 ：5.3
引用产品： 人Ⅱ型肺泡上皮细胞
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