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产品概述
名称 | NCI-H716 [H716] (人结直肠腺癌细胞) (STR鉴定正确) |
别称 | NCI H716; H716; H-716; NCIH716 |
种属 | 人 |
生长特性 | 半贴半悬 |
细胞形态 | 上皮细胞,悬浮并部分聚团生长 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2-3次/周 |
背景描述 | NCI-H716 [H716]细胞是从一位经5-氟尿嘧啶治疗的患者腹水中得到的细胞建立的细胞株。NCI-H716 [H716]细胞与其它结直肠癌细胞系不同,它有多巴脱羧酶,细胞质中有核心致密的内分泌型颗粒。NCI-H716 [H716]细胞不表达TAG-72或CA19-9抗原,也不生成癌胚抗原(CEA)。 |
年龄(性别) | 男性;33岁 |
组织来源 | 器官:盲肠;疾病:结直肠腺癌 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肠癌细胞 |
生物安全等级 | BSL-1 |
倍增时间 | ~50 hours |
致瘤性 | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10^7 cells). |
保藏机构 | ATCC; CCL-251 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 11 D2S1338 14,16 D3S1358 16,18 D5S818 11 D7S820 10,11 D8S1179 15 D13S317 8,11 D16S539 11,12 D18S51 15,16 D19S433 15,18.2 D21S11 32.2 FGA 20,22 PentaD 9 PentaE 7,12 TH01 6,9.3 TPOX 8,11 vWA 16,17 D6S1043 12,13 D12S391 18,19 D2S441 11 -
STR鉴定图
参考文献
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Transepithelial Transport of the Bifunctional Peptide IPYWTY Indirectly Induced Insulin Release Mediated by Active GLP-1 (2023-08-17)
作者:Mingkai Zhang:1.National Engineering Research Center for Functional Food, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China. ; Ling Zhu:1.National Engineering Research Center for Functional Food, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China. ; Hui Zhang:1.National Engineering Research Center for Functional Food, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China. ; Xingguo Wang:1.National Engineering Research Center for Functional Food, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, 1800
期刊:Transepithelial Transport of the Bifunctional Peptide IPYWTY Indirectly Induced Insulin Release Mediated by Active GLP-1
影响因子 :6.1
引用产品: Beta-TC-6 细胞 , NCI-H716 [H716] 细胞
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Evaluating the In Situ Insulinotropic Effects of Pea Protein Hydrolysates Mediated by Active GLP-1 via a 2D and Dual-Layered Coculture Cell Model (2023-09-18)
作者:Mingkai Zhang:1.National Engineering Research Center for Functional Food, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China. ; Ling Zhu:1.National Engineering Research Center for Functional Food, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China. ; Hui Zhang:1.National Engineering Research Center for Functional Food, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China. ; Xingguo Wang:1.National Engineering Research Center for Functional Food, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, 1800
期刊:Evaluating the In Situ Insulinotropic Effects of Pea Protein Hydrolysates Mediated by Active GLP-1 via a 2D and Dual-Layered Coculture Cell Model
影响因子 :6.1
引用产品: Beta-TC-6 细胞 , NCI-H716 [H716] 细胞
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