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![Sp2/0-Ag14[SP2/0] (小鼠骨髓瘤细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0445-1.jpg)
![Sp2/0-Ag14[SP2/0] (小鼠骨髓瘤细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0445-2.jpg)
![Sp2/0-Ag14[SP2/0] (小鼠骨髓瘤细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0445-3.jpg)
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产品概述
| 名称 | Sp2/0-Ag14[SP2/0] (小鼠骨髓瘤细胞) (STR鉴定正确) |
| 别称 | SP2/0-Ag14; SP2/0-AG14; SP2/0-ag14; Sp2/O-Ag14; SP2/O-Ag14; Sp2/0-Ag-14; SP2-0-Ag14; SP2/0 Ag-14; SP-2/0-AG14; Sp 2/0-Ag 14; Sp2/0; SP2/0; Sp2/O; SP2/O; SP-2; SP2; GM03569; GM03569B; GM3569 |
| 种属 | 小鼠 |
| 生长特性 | 悬浮细胞 |
| 细胞形态 | 淋巴母细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 3×10^5-5×10^5cells/mL |
| 推荐换液频率 | 2-3次/周 |
| 注意事项 | 该细胞为悬浮细胞，请注意离心收集细胞悬液；请勿直接倒掉细胞培养液。 |
| 背景描述 | Sp2/0-Ag14细胞是由绵羊红细胞免疫的BALB/c小鼠脾细胞和P3X63Ag8骨髓瘤细胞融合得到的。Sp2/0-Ag14细胞不分泌免疫球蛋白，对20μg/ml的8-氮鸟嘌呤有抗性，对HAT比较敏感；Sp2/0-Ag14细胞可以作为细胞融合时的B细胞组分用于制备杂交瘤；鼠痘病毒阴性。 |
| 组织来源 | 脾脏 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 骨髓瘤细胞 |
| 生物安全等级 | BSL-1 |
| 抗原表达情况 | H-2d |
| 保藏机构 | ATCC; CRL-1581 ATCC;CRL-8287 DSMZ;ACC-146 ECACC;85072401 |
STR鉴定
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STR位点信息
18-3 18,19 6-7 11,12 5-5 13,14 X-1 25 1-2 16,17,19 7-1 26.2,28 8-1 13 1-1 15,16,17 19-2 12,13 15-3 22.3,23.3 12-1 15,16,17 6-4 17,18,19 4-2 21.3,22.3 3-2 13,14 2-1 16 13-1 16.2
11-2 17,18 17-2 16,17 -
STR鉴定图
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参考文献
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Generation of a blockage monoclonal antibody of LILRB1 against HLA-G (2023-09-06)
作者：Yunlong Shen:1.State Key Laboratory of Biotherapy and Cancer Center/Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China. Electronic address: shenyunlongft@163.com. ; Ruirui Zhang:1.State Key Laboratory of Biotherapy and Cancer Center/Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China. Electronic address: 1741262464@qq.com. ; Xiaohua Jiang:1.State Key Laboratory of Biotherapy and Cancer Center/Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China. Electronic address: 1258758932@qq.com. ; Jinliang Yang:1.State Key Laboratory of Biotherapy and Cancer Center/Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China. Electronic address: jlyang01@163.com. ;
期刊：Generation of a blockage monoclonal antibody of LILRB1 against HLA-G
影响因子 ：1.6
引用产品： CHO-K1 细胞 , CHO 细胞 , 293T [HEK-293T] 细胞 , Sp2/0-Ag14[SP2/0] 细胞
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Anti‑Axl monoclonal antibodies attenuate the migration of MDA‑MB‑231 breast cancer cells (2008-01-20)
作者：Hong Chang:1.Institute of Medical Technology, Suzhou Vocational Health College, Suzhou, Jiangsu 215009, P.R. China. ; 2.College of Basic Medicine, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050200, P.R. China. ; Ran An:1.College of Basic Medicine, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050200, P.R. China. ; 2.Hebei Key Laboratory of Chinese Medicine Research on Cardio-cerebrovascular Disease, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050051, P.R. China. ; Xinying Li:1.State Key Laboratory of Toxicology and Medical Countermeasures, Beijing Institute of Pharmacology and Toxicology, Beijing 100850, P.R. China. ; Xiaoling Lang:1.Beijing Key Laboratory of Therapeutic Gene Engineering Antibody, Beijing 100850, P.R. China. ; Jiannan Feng:1.State Key Laboratory of Toxicology and Medical Countermeasures, Beijing Institute of Pharmacology and Toxicology, Beijing 100850, P.R. China. ; 2.Beijing Key Laboratory of Therapeutic Gene Engineering An
期刊：Anti‑Axl monoclonal antibodies attenuate the migration of MDA‑MB‑231 breast cancer cells
影响因子 ：2.9
引用产品： Sp2/0-Ag14[SP2/0] 细胞
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Pumping-controlled multicolor modulation of upconversion emission for dual-mode dynamic anti-counterfeiting (2006-00-20)
作者：Xiaoru Dai: 1.Institute of Optoelectronic Materials and Devices, China Jiliang University, Hangzhou, 310018, PR China; Ke Wang: 1.School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, PR China; Lei Lei: 1.Institute of Optoelectronic Materials and Devices, China Jiliang University, Hangzhou, 310018, PR China; Shiqing Xu: 1.Institute of Optoelectronic Materials and Devices, China Jiliang University, Hangzhou, 310018, PR China; Yao Cheng: 1.CAS Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Key Laboratory of Nanomaterials, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian, 350002, PR China; Yuansheng Wang: 1.CAS Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Key Laboratory of Nanomaterials, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian, 350002, PR China;
期刊：Pumping-controlled multicolor modulation of upconversion emission for dual-mode dynamic anti-counterfeiting
影响因子 ：7.5
引用产品： MEM (含NEAA) 培养基 , MCF7 [MCF-7] 细胞 , Sp2/0-Ag14[SP2/0] 细胞
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