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| 名称 | Reh (人急性非B非T淋巴细胞白血病) (STR鉴定正确) |
| 别称 | REH |
| 种属 | 人 |
| 生长特性 | 悬浮细胞 |
| 细胞形态 | 淋巴母细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
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| 推荐传代比例 | 3×10^5-5×10^5cells/mL |
| 推荐换液频率 | 2-3次/周 |
| 注意事项 | 该细胞为悬浮细胞，请注意离心收集细胞悬液；请勿直接倒掉细胞培养液。 |
| 背景描述 | CD3A(17%)、CD3B(17%)、CD3C(20%)，CD4(15%)，CD10(55%) |
| 年龄（性别） | 女性；15岁 |
| 组织来源 | 外周血 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 白血病细胞 |
| 生物安全等级 | BSL-1 |
| 倍增时间 | ~50-70 hours |
| 保藏机构 | ATCC; CRL-8286 DSMZ; ACC-22 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 11,12 D2S1338 20,22 D3S1358 18 D5S818 11,13 D7S820 9,12 D8S1179 13,15 D13S317 11,13 D16S539 9 D18S51 10,14 D19S433 12,14 D21S11 28,29,30 FGA 22,23 PentaD 9,11 PentaE 7,11 TH01 7 TPOX 8 vWA 14,15 D6S1043 11,21 D12S391 23,25 D2S441 10,14 -
STR鉴定图
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参考文献
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Histidine re-sensitizes pediatric acute lymphoblastic leukemia to 6-mercaptopurine through tetrahydrofolate consumption and SIRT5-mediated desuccinylation (2024-03-14)
作者：Na Dong:1.Department of Pediatrics, Qilu Hospital of Shandong University, Jinan, 250012, Shandong Province, China. ; Hui-Xian Ma:1.Cryomedicine Laboratory, Qilu Hospital of Shandong University, Jinan, 250012, Shandong Province, China. ; Xue-Qin Liu:1.Department of Pediatrics, Qilu Hospital of Shandong University, Jinan, 250012, Shandong Province, China. ; Dong Li:1.Cryomedicine Laboratory, Qilu Hospital of Shandong University, Jinan, 250012, Shandong Province, China. ; Ling-Hong Liu:1.Cryomedicine Laboratory, Qilu Hospital of Shandong University, Jinan, 250012, Shandong Province, China. ; Qing Shi:1.Cryomedicine Laboratory, Qilu Hospital of Shandong University, Jinan, 250012, Shandong Province, China. ; Xiu-Li Ju:1.Department of Pediatrics, Qilu Hospital of Shandong University, Jinan, 250012, Shandong Province, China. jxlqlyy@163.com. ; 2.Cryomedicine Laboratory, Qilu Hospital of Shandong University, Jinan, 250012, Shandong Province, China. jxlqlyy@163.com. ;
期刊：Histidine re-sensitizes pediatric acute lymphoblastic leukemia to 6-mercaptopurine through tetrahydrofolate consumption and SIRT5-mediated desuccinylation
DOI：10.1038/s41419-024-06599-5
影响因子 ：9.0
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1,5-Anhydroglucitol promotes pre-B acute lymphocytic leukemia progression by driving glycolysis and reactive oxygen species formation (2023-02-06)
作者：Huasu Zhu:1.Department of Pediatrics, Qilu Hospital of Shandong University, Jinan, 250012, Shandong Province, China. ; Huixian Ma:1.Laboratory of Cryomedicine, Qilu Hospital of Shandong University, Jinan, 250012, Shandong Province, China. ; Na Dong:1.Department of Pediatrics, Qilu Hospital of Shandong University, Jinan, 250012, Shandong Province, China. ; Min Wu:1.Department of Pediatrics, Qilu Hospital of Shandong University, Jinan, 250012, Shandong Province, China. ; Dong Li:1.Laboratory of Cryomedicine, Qilu Hospital of Shandong University, Jinan, 250012, Shandong Province, China. ; Linghong Liu:1.Laboratory of Cryomedicine, Qilu Hospital of Shandong University, Jinan, 250012, Shandong Province, China. ; Qing Shi:1.Laboratory of Cryomedicine, Qilu Hospital of Shandong University, Jinan, 250012, Shandong Province, China. ; Xiuli Ju:1.Department of Pediatrics, Qilu Hospital of Shandong University, Jinan, 250012, Shandong Province, China. jxlqlyy@163.com. ; 2.Laboratory of Cryom
期刊：1,5-Anhydroglucitol promotes pre-B acute lymphocytic leukemia progression by driving glycolysis and reactive oxygen species formation
DOI：10.1186/s12885-023-10589-9
影响因子 ：3.8
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1,5-AG suppresses pro-inflammatory polarization of macrophages and promotes the survival of B-ALL in vitro by upregulating CXCL14 (2023-05-11)
作者：Min Wu:1.Department of Pediatrics, Qilu Hospital of Shandong University, Jinan 250012, China. ; Shule Zhang:1.Department of Pediatrics, Qilu Hospital of Shandong University, Jinan 250012, China. ; Cheng Chi:1.Department of Pediatrics, Qilu Hospital of Shandong University, Jinan 250012, China. ; Huasu Zhu:1.Department of Pediatrics, Qilu Hospital of Shandong University, Jinan 250012, China. ; Huixian Ma:1.Cryomedicine Laboratory, Qilu Hospital of Shandong University, Jinan 250012, China. ; Linghong Liu:1.Cryomedicine Laboratory, Qilu Hospital of Shandong University, Jinan 250012, China. ; Qing Shi:1.Cryomedicine Laboratory, Qilu Hospital of Shandong University, Jinan 250012, China. ; Dong Li:1.Cryomedicine Laboratory, Qilu Hospital of Shandong University, Jinan 250012, China. ; Xiuli Ju:1.Department of Pediatrics, Qilu Hospital of Shandong University, Jinan 250012, China; Cryomedicine Laboratory, Qilu Hospital of Shandong University, Jinan 250012, China. Electronic address: jxlq
期刊：1,5-AG suppresses pro-inflammatory polarization of macrophages and promotes the survival of B-ALL in vitro by upregulating CXCL14
DOI：10.1016/j.molimm.2023.05.003
影响因子 ：3.6
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Knockout KHDRBS3 inhibits cell proliferation and glucocorticoid resistance in acute lymphoblastic leukemia (2022-10-01)
作者：**ncui Lu: 1.Department of Pediatrics, The Affiliated Huaian No.1 People’s Hospital of Nan**g Medical University, No. 1, Huanghe West Road, Huaiyin District, Huaian City, 223300, Jiangsu Province, China; Li Ma: 1.Department of Pediatrics, The Affiliated Huaian No.1 People’s Hospital of Nan**g Medical University, No. 1, Huanghe West Road, Huaiyin District, Huaian City, 223300, Jiangsu Province, China; Yufang Yuan: 1.Department of Pediatrics, The Affiliated Huaian No.1 People’s Hospital of Nan**g Medical University, No. 1, Huanghe West Road, Huaiyin District, Huaian City, 223300, Jiangsu Province, China;
期刊：Knockout KHDRBS3 inhibits cell proliferation and glucocorticoid resistance in acute lymphoblastic leukemia
DOI：10.1007/s13273-022-00299-x
影响因子 ：1.7
引用产品： Reh 细胞 , CEM/C1 细胞 , CCRF-CEM [CCRF CEM] 细胞 , BALL-1 细胞 , RPMI-1640 培养基 , 青霉素-链霉素溶液 (双抗)，100×
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