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产品概述
名称 | CHO/dhFr- [CHO-DXB11] (仓鼠卵巢细胞(二氢叶酸还原酶缺陷))(种属鉴定正确) |
种属 | 中国仓鼠 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2-3次/周 |
背景描述 | CHO/dhFr-细胞为二氢叶酸还原酶缺陷细胞。在没有HT(次黄嘌呤-胸腺嘧啶)时,CHO/dhFr-细胞会死亡;CHO/dhFr-细胞生长培养基中加氨甲喋呤,可以防止对药物低抗性的回变细胞的生长。 |
年龄(性别) | 雌性;成年 |
组织来源 | 正常卵巢 |
细胞类型 | 自发永生化细胞 |
生物安全等级 | BSL-1 |
种属鉴定
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种属鉴定图
参考文献
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Wuzi Yanzong Pill protects neural tube defects by activating PI3K/Akt signaling pathway (2023-05-21)
作者:Xinliang Wang:1.The Key Research Laboratory of Benefiting Qi for Acting Blood Circulation Method to Treat Multiple Sclerosis of State Administration of Traditional Chinese Medicine/Neurobiology Research Center, Shanxi University of Chinese medicine, Jinzhong, China. ; Chanjuan Yang:1.The Key Research Laboratory of Benefiting Qi for Acting Blood Circulation Method to Treat Multiple Sclerosis of State Administration of Traditional Chinese Medicine/Neurobiology Research Center, Shanxi University of Chinese medicine, Jinzhong, China. ; Yanrong Li:1.The Key Research Laboratory of Benefiting Qi for Acting Blood Circulation Method to Treat Multiple Sclerosis of State Administration of Traditional Chinese Medicine/Neurobiology Research Center, Shanxi University of Chinese medicine, Jinzhong, China. ; Qiang Gong:1.The Key Research Laboratory of Benefiting Qi for Acting Blood Circulation Method to Treat Multiple Sclerosis of State Administration of Traditional Chinese Medicine/Neurobiology Re
期刊:Wuzi Yanzong Pill protects neural tube defects by activating PI3K/Akt signaling pathway
影响因子 :1.8
引用产品: CHO 细胞 , CHO/dhFr- [CHO-DXB11] 细胞 , IMDM 培养基 , 特级胎牛血清 , 青霉素-链霉素-谷氨酰胺溶液 (100×)
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