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产品概述
名称 | RBL-2H3 (大鼠嗜碱性细胞白血病细胞) (种属鉴定正确) |
别称 | RBL2H3; RBL 2H3; RBL.2H3 |
种属 | 大鼠 |
生长特性 | 贴壁细胞 |
细胞形态 | 成纤维细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
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推荐传代比例 | 1:3-1:6 |
推荐换液频率 | 2-3次/周 |
背景描述 | RBL-2H3细胞是1978年国立牙科研究所的免疫学实验室从Wistar大鼠保持肿瘤状态的嗜碱性细胞中分离和克隆出来的嗜碱性白血病细胞株。RBL-2H3细胞具有高亲和力的IgE受体,通过集聚这些受体或与钙离子载体协同作用可以激活它们分泌组胺及其他递质。RBL-2H3细胞广泛地用于研究肥大细胞FcERI和分泌的生化途径。RBL-2H3细胞是研究FcERI结构的模型。它们广泛地用于研究细胞分泌的不同方面,包括细胞内钙浓度改变、磷酸脂酶激活、蛋白激酶和小G蛋白的作用。虽然几乎所有批号的FBS都支持细胞的生长,但在某些批号中FcERI集聚后脱粒化得更好。另一株大鼠嗜碱性细胞株(RBL)不会脱粒化。 |
组织来源 | 外周血;化学诱变嗜碱性细胞;白血病 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 白血病细胞 |
生物安全等级 | BSL-1 |
倍增时间 | ~50-60 hours |
受体表达情况 | FcERI (Fc of IgE) |
基因表达情况 | histamine |
保藏机构 | ATCC; CRL-2256 DSMZ; ACC-312 |
种属鉴定
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种属鉴定图
参考文献
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Assessment of the effect of glycation on the allergenicity of sesame proteins (2023-03-26)
作者:Songsong Jiang:1.College of Tourism and Culinary Science, Yangzhou University, Yangzhou, Jiangsu 225127, China. Electronic address: 17600189209@163.com. ; Tao Wang:1.College of Tourism and Culinary Science, Yangzhou University, Yangzhou, Jiangsu 225127, China. ; Kuan Chen:1.College of Tourism and Culinary Science, Yangzhou University, Yangzhou, Jiangsu 225127, China. ; Hengpeng Wang:1.College of Tourism and Culinary Science, Yangzhou University, Yangzhou, Jiangsu 225127, China. ; Xiangren Meng:1.College of Tourism and Culinary Science, Yangzhou University, Yangzhou, Jiangsu 225127, China; Chinese Cuisine Promotion and Research Base, Yangzhou University, Yangzhou 225127, China. ;
期刊:Assessment of the effect of glycation on the allergenicity of sesame proteins
DOI:10.1016/j.foodres.2023.112771
影响因子 :8.1
引用产品: 特级胎牛血清 , 青霉素-链霉素-两性霉素B溶液 (100×) , RBL-2H3 细胞
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Insight into the conformational and allergenicity alterations of shrimp tropomyosin induced by Sargassum fusiforme polyphenol (2023-01-25)
作者:Yiming Zhao:1.Laboratory of Food Safety, College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China. ; Ziye Zhang:1.Laboratory of Food Safety, College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China. Electronic address: zhangziye@ouc.edu.cn. ; Zhenxing Li:1.Laboratory of Food Safety, College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China. Electronic address: lizhenxing@ouc.edu.cn. ; Xiu-Min Li:1.Department of Pathology, Microbiology and Immunology and Department of Otolaryngology, School of Medicine, New York Medical College, Valhalla, NY 10595, USA. ; Hao Wang:1.Laboratory of Food Safety, College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China. ; Hong Lin:1.Laboratory of Food Safety, College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China. ;
期刊:Insight into the conformational and allergenicity alterations of shrimp tropomyosin induced by Sargassum fusiforme polyphenol
DOI:10.1016/j.foodres.2023.112521
影响因子 :8.1
引用产品: RBL-2H3 细胞
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Modulation of soy protein immunoreactivity by different matrix structures of lactic acid bacterium-induced soy protein gels: Epitope destruction during in vitro gastroduodenal digestion and absorption (2023-07-16)
作者:Yaqiong Wang:1.College of Food Science and Technology, Nanjing Agricultural University, Jiangsu Province, PR China. ; Yumeng Fu:1.College of Food Science and Technology, Nanjing Agricultural University, Jiangsu Province, PR China. ; Wei Li:1.College of Food Science and Technology, Nanjing Agricultural University, Jiangsu Province, PR China. ; Benjamin K Simpson:1.Department of Food Science and Agricultural Chemistry, McGill University, Macdonald, QC, Canada. ; Xin Rui:1.College of Food Science and Technology, Nanjing Agricultural University, Jiangsu Province, PR China. Electronic address: ruix@njau.edu.cn. ;
期刊:Modulation of soy protein immunoreactivity by different matrix structures of lactic acid bacterium-induced soy protein gels: Epitope destruction during in vitro gastroduodenal digestion and absorption
DOI:10.1016/j.foodres.2023.113281
影响因子 :8.1
引用产品: RBL-2H3 细胞
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Molecular docking, network pharmacology and experimental verification to explore the mechanism of Wulongzhiyangwan in the treatment of pruritus (2023-01-07)
作者:Lyu Anqi:1.School of Medicine, Xiamen University, Xiamen, 361000, Fujian, China. ; Shan Shijun:1.Department of Dermatology, Xiang'an Hospital of Xiamen University, 2000 Xiang'an East Road, Xiamen, 361000, Fujian, China. 18750209079@139.com. ;
期刊:Molecular docking, network pharmacology and experimental verification to explore the mechanism of Wulongzhiyangwan in the treatment of pruritus
DOI:10.1038/s41598-023-27593-5
影响因子 :4.6
引用产品: RBL-2H3 细胞
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Anti-Allergic Effect of Dietary Polyphenols Curcumin and Epigallocatechin Gallate via Anti-Degranulation in IgE/Antigen-Stimulated Mast Cell Model: A Lipidomics Perspective (2023-05-05)
作者:Jun Zeng:1.College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China. ; 2.Xiamen Key Laboratory of Marine Functional Food, Xiamen 361021, China. ; Jingwen Hao:1.College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China. ; Zhiqiang Yang:1.College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China. ; Chunyu Ma:1.College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China. ; Longhua Gao:1.College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China. ; Yue Chen:1.The Affiliated Stomatology Hospital, School of Medicine, Zhejiang University, Hangzhou 310000, China. ; Guiling Li:1.College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China. ; 2.Xiamen Key Laboratory of Marine Functional Food, Xiamen 361021, China. ; Jia Li:1.Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea R
期刊:Anti-Allergic Effect of Dietary Polyphenols Curcumin and Epigallocatechin Gallate via Anti-Degranulation in IgE/Antigen-Stimulated Mast Cell Model: A Lipidomics Perspective
影响因子 :4.1
引用产品: RBL-2H3 细胞
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A fibrin Site-Specific Nanoprobe for Imaging Fibrin-Rich Thrombi and Preventing Thrombus Formation in Venous Vessels (2003-02-20)
作者:Yixin Zhong:1.Department of Radiology, Second Affiliated Hospital of Chongqing Medical University, No. 74 Linjiang Rd, Yuzhong District, Chongqing, 400010, P. R. China. ; 2.CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China, No. 11, First North Road, Zhongguancun, Beijing, 100190, P. R. China. ; 3.University of Chinese Academy of Sciences, Beijing, 100049, P. R. China. ; 4.State Key Laboratory of Ultrasound in Medicine and Engineering, Chongqing Key Laboratory of Ultrasound Molecular Imaging & Ultrasound Department of the Second Affiliated Hospital of Chongqing Medical University, No. 74 Linjiang Rd, Yuzhong District, Chongqing, 400010, P. R. China. ; Man Ye:1.Department of Radiology, Renmin Hospital of Wuhan University, No. 238 Jiefang Rd, Wuchang District, Wuhan, Hubei, 430060, P. R. China. ; Liandi Huang:1.State Key Laboratory of Ultrasound in Medicine and Engi
期刊:A fibrin Site-Specific Nanoprobe for Imaging Fibrin-Rich Thrombi and Preventing Thrombus Formation in Venous Vessels
影响因子 :32.1
引用产品: RBL-2H3 细胞
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A Metabolomics Study on the Bone Protective Effects of a Lignan-Rich Fraction From Sambucus Williamsii Ramulus in Aged Rats (2008-08-20)
作者:Hui-Hui Xiao:1.State Key Laboratory of Chinese Medicine and Molecular Pharmacology (Incubation), Hong Kong Polytechnic University Shenzhen Research Institute, Shenzhen, China. ; Tung-Ting Sham:1.Department of Applied Biology and Chemical Technology, Hong Kong Polytechnic University, Hong Kong, China. ; Chi-On Chan:1.State Key Laboratory of Chinese Medicine and Molecular Pharmacology (Incubation), Hong Kong Polytechnic University Shenzhen Research Institute, Shenzhen, China. ; 2.Department of Applied Biology and Chemical Technology, Hong Kong Polytechnic University, Hong Kong, China. ; Meng-Heng Li:1.Department of Applied Biology and Chemical Technology, Hong Kong Polytechnic University, Hong Kong, China. ; Xi Chen:1.School of Biotechnology, East China University of Science and Technology, Shanghai, China. ; Qing-Chang Wu:1.Institute of Traditional Chinese Medicine & Natural Products, Jinan University, Guangzhou, China. ; Daniel Kam-Wah Mok:1.State Key Laboratory of Chinese Medici
期刊:A Metabolomics Study on the Bone Protective Effects of a Lignan-Rich Fraction From Sambucus Williamsii Ramulus in Aged Rats
影响因子 :5.6
引用产品: RBL-2H3 细胞
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