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产品概述
| 名称 | Sf9 (昆虫卵巢细胞) (种属鉴定正确) |
| 别称 | SF9; sf9; SF-9; Sf-9; sf-9; Sf 9; Spodoptera frugiperda clone 9; Sf clone 9; IPLB-Sf-9AE; IPLB-SF-9AE; IPLB-SF-9; IPLB-Sf-9; IPLB-Sf9 |
| 种属 | 昆虫 |
| 生长特性 | 半贴半悬 |
| 细胞形态 | 圆形 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
培养条件:
气相：空气，100%, 温度：28℃
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| 推荐传代比例 | 5×10^5-1×10^6cells/mL |
| 推荐换液频率 | 2-3次/周 |
| 注意事项 | 该细胞为28℃培养，不通二氧化碳，请提前准备专用培养箱。 |
| 背景描述 | Sf9细胞是源于雌性草地贪夜蛾蛹的卵巢组织，可以用于复制杆状病毒表达载体。 |
| 年龄（性别） | 蛹 |
| 组织来源 | 卵巢 |
| 细胞类型 | 自发永生化细胞 |
| 生物安全等级 | BSL-1 |
| 倍增时间 | ~27-50 hours |
| 保藏机构 | ATCC; CRL-1711 DSMZ; ACC-125 ECACC; 89070101 |
种属鉴定
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种属鉴定图
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参考文献
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A DNA Plasmid-Based Approach for Efficient Synthesis of Sacbrood Virus Infectious Clones within Host Cells (2023-09-01)
作者：Dandan Yue:1.College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350002, China. ; Runlin Li:1.College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350002, China. ; Jikailang Zhang:1.College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350002, China. ; Yanping Chen:1.Bee Research Laboratory, Agriculture Research Service, USDA, Beltsville, MD 20705, USA. ; Evan C Palmer-Young:1.Bee Research Laboratory, Agriculture Research Service, USDA, Beltsville, MD 20705, USA. ; Shaokang Huang:1.College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350002, China. ; 2.Honeybee Biology Observation Station, Ministry of Agriculture and Rural Affairs, Fuzhou 350002, China. ; Wei-Fone Huang:1.College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 35000
期刊：A DNA Plasmid-Based Approach for Efficient Synthesis of Sacbrood Virus Infectious Clones within Host Cells
影响因子 ：4.7
引用产品： Sf9 细胞
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Design, synthesis and insecticidal activity and mechanism research of Chasmanthinine derivatives (2022-09-10)
作者：Ziyu Song:1.School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, Sichuan, People's Republic of China. ; Xiangyu Li:1.School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, Sichuan, People's Republic of China. ; Ke Xu:1.School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, Sichuan, People's Republic of China. ; Guoqing Sun:1.School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, Sichuan, People's Republic of China. ; Liu Yang:1.School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, Sichuan, People's Republic of China. ; Linyu Huang:1.School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, Sichuan, People's Republic of China. ; Junqi Liu:1.School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, Sichuan, People's Republic of China. ; Pengyuan Yin:1.Schoo
期刊：Design, synthesis and insecticidal activity and mechanism research of Chasmanthinine derivatives
DOI：10.1038/s41598-022-19523-8
影响因子 ：5.0
引用产品： Sf9 细胞 , MTT溶液 (5mg/mL) , DMSO
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High cell density fed-batch production of insecticidal recombinant ribotoxin hirsutellin A from Pichia pastoris (2009-08-20)
作者：Hongbo Li:1.Postdoctoral Mobile Station of Biology, Genetic Engineering Research Center, College of Life Sciences, Chongqing University, Chongqing, 400030, China. ; 2.College of Biological and Food Engineering, Huaihua University, Huaihua, 418008, China. ; Yuxian Xia:1.Postdoctoral Mobile Station of Biology, Genetic Engineering Research Center, College of Life Sciences, Chongqing University, Chongqing, 400030, China. yuxianxia@cqu.edu.cn. ; 2.Genetic Engineering Research Center, College of Life Sciences, Chongqing University, No. 55 South Road of University Town, Shapingba District, Chongqing, 401331, China. yuxianxia@cqu.edu.cn. ;
期刊：High cell density fed-batch production of insecticidal recombinant ribotoxin hirsutellin A from Pichia pastoris
影响因子 ：6.4
引用产品： Sf9 细胞
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