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产品概述
| 名称 | NIH/3T3 (小鼠胚胎细胞) (STR鉴定正确) |
| 别称 | NIH/3T3; NIH-3T3; NIH3T3; 3T3; 3T3NIH; 3T3-Swiss; Swiss-3T3; Swiss/3T3; Swiss 3T3; Swiss3T3 |
| 种属 | 小鼠 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 成纤维细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:3-1:6 |
| 推荐换液频率 | 2-3次/周 |
| 背景描述 | NIH/3T3细胞是从NIH Swiss小鼠胚胎培养物中建立的高度接触性抑制的连续传代细胞株。为了培育在形态学特征上更适合于进行转化分析的亚株，建立的NIH/3T3细胞株又进行了5轮以上亚克隆。NIH/3T3细胞对肉瘤病毒的转化灶形成和白血病病毒的繁殖高度敏感，对DNA转化及转染研究十分有用；NIH/3T3细胞鼠痘病毒阴性。 |
| 年龄（性别） | 胚胎 |
| 组织来源 | 胚胎 |
| 细胞类型 | 自发永生化细胞 |
| 生物安全等级 | BSL-1 |
| 倍增时间 | ~20 hours |
| 保藏机构 | ATCC; CRL-1658 ATCC; CRL-6442DSMZ; ACC-59 ECACC; 93061524 |
STR鉴定
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STR位点信息
18-3 17,19 6-7 12 5-5 14,15 X-1 25 1-2 13,17 7-1 29 8-1 15 1-1 10 19-2 11,12 15-3 20.3 12-1 20 6-4 16 4-2 19.3,20.3 3-2 14,15 2-1 9 13-1 16.2
11-2 15,17 17-2 13,14 -
STR鉴定图
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参考文献
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High-throughput and proteome-wide discovery of endogenous biomolecular condensates (2024-03-18)
期刊：Nature Chemistry
DOI：10.1038/s41557-024-01485-1
影响因子 ：21.8
引用产品： NCI-H1975 细胞 , NIH/3T3 细胞
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Spatiotemporal transformable nano-assembly for on-demand drug delivery to enhance anti-tumor immunotherapy (2024-02-14)
期刊：Asian Journal of Pharmaceutical Sciences
DOI：10.1016/j.ajps.2024.100888
影响因子 ：10.2
引用产品： NIH/3T3 细胞
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Enzyme-Responsive Modular Peptides Enhance Tumor Penetration of Quantum Dots via Charge Reversal Strategy (2024-01-27)
期刊：ACS Applied Materials & Interfaces
影响因子 ：9.5
引用产品： NIH/3T3 细胞
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MMP14high macrophages orchestrate progressive pulmonary fibrosis in SR-Ag-induced hypersensitivity pneumonitis (2024-01-11)
期刊：PHARMACOLOGICAL RESEARCH
DOI：10.1016/j.phrs.2024.107070
影响因子 ：9.3
引用产品： NIH/3T3 细胞 , 青霉素-链霉素溶液 (双抗)，100×
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Gelatin-based dynamic response antioxidant, anti-inflammatory multifunctional hydrogel for enhanced diabetic wound repair (2024-01-20)
期刊：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI：10.1016/j.ijbiomac.2024.129453
影响因子 ：8.2
引用产品： NIH/3T3 细胞 , RAW 264.7 细胞
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Doping proanthocyanidins into gel/zirconium hybrid hydrogel to reshape the microenvironment of diabetic wounds for healing acceleration (2024-01-17)
期刊：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI：10.1016/j.ijbiomac.2024.129353
影响因子 ：8.2
引用产品： NIH/3T3 细胞 , RAW 264.7 细胞
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Conductive interpenetrating network organohydrogels of gellan gum/polypyrrole with weather-tolerance, piezoresistive sensing and shape-memory capability (2024-02-15)
期刊：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI：10.1016/j.ijbiomac.2024.130215
影响因子 ：8.2
引用产品： NIH/3T3 细胞 , NIH/3T3细胞专用培养基
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Faster structured illumination microscopy using complementary encoding-based compressive imaging (2024-03-25)
期刊：Photonics Research
影响因子 ：7.6
引用产品： NIH/3T3 细胞
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Polyhexamethylene guanidine accelerates the macrophage foamy formation mediated pulmonary fibrosis (2024-02-13)
期刊：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
DOI：10.1016/j.ecoenv.2024.116084
影响因子 ：6.8
引用产品： MH-S 细胞 , NIH/3T3 细胞
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Sawdust-derived cellulose nanofibrils with high biosafety for potential bioprinting (2024-01-09)
期刊：INDUSTRIAL CROPS AND PRODUCTS
DOI：10.1016/j.indcrop.2024.118025
影响因子 ：5.9
引用产品： NIH/3T3 细胞
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Biocompatible Bletilla Striata polysaccharide-based hemostatic sponges enhance wound healing and reduce the risk for infections (2024-03-22)
期刊：Carbohydrate Polymer Technologies and Applications
DOI：10.1016/j.carpta.2024.100492
影响因子 ：5.5
引用产品： NIH/3T3 细胞
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Tetrandrine Alleviates Silica-induced Pulmonary Fibrosis Through PI3K/AKT Pathway: Network Pharmacology Investigation and Experimental Validation (2024-01-24)
期刊：INFLAMMATION
DOI：10.1007/s10753-023-01964-6
影响因子 ：5.1
引用产品： NIH/3T3 细胞
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Modulation of autophagy affected tumorigenesis induced by the envelope glycoprotein of JSRV (2024-03-13)
期刊：VIROLOGY
DOI：10.1016/j.virol.2024.110059
影响因子 ：3.7
引用产品： NIH/3T3 细胞
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Study on Antibacterial and Biocompatibility of Thermally Crosslinked Polyvinylpyrrolidone Nanofiber Iodine Complexes (2024-02-08)
期刊：CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
影响因子 ：3.1
引用产品： NIH/3T3 细胞
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A self-monitoring microneedle patch for light-controlled synergistic treatment of melanoma (2023-03-27)
期刊：Bioactive Materials
DOI：10.1016/j.bioactmat.2023.03.016
影响因子 ：18.9
引用产品： B16-F10 细胞 , NIH/3T3 细胞
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Endothelial discoidin domain receptor 1 senses flow to modulate YAP activation (2023-10-13)
期刊：Nature Communications
DOI：10.1038/s41467-023-42341-z
影响因子 ：16.6
引用产品： EA.hy926 细胞 , NIH/3T3 细胞
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The miR-669a-5p/G3BP/HDAC6/AKAP12 Axis Regulates Primary Cilia Length (2023-12-13)
期刊：Advanced Science
影响因子 ：15.1
引用产品： NIH/3T3 细胞
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MOFs-Based Nanoagents Enable Sequential Damage to Cancer-Associated Fibroblast and Tumor Cells for Phototriggered Tumor Microenvironment Regulation (2023-08-31)
期刊：Small
影响因子 ：13.3
引用产品： 4T1 细胞 , NIH/3T3 细胞 , C2C12 细胞
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Injectable chitosan/xyloglucan composite hydrogel with mechanical adaptivity and endogenous bioactivity for skin repair (2023-04-13)
期刊：CARBOHYDRATE POLYMERS
DOI：10.1016/j.carbpol.2023.120904
影响因子 ：11.2
引用产品： NIH/3T3 细胞
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Biocompatible and biodegradable chitin-based hydrogels crosslinked by BDDE with excellent mechanical properties for effective prevention of postoperative peritoneal adhesion (2023-01-05)
期刊：CARBOHYDRATE POLYMERS
DOI：10.1016/j.carbpol.2023.120543
影响因子 ：11.2
引用产品： NIH/3T3 细胞
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Multifunctional MOF-Based Microneedle Patch With Synergistic Chemo-Photodynamic Antibacterial Effect and Sustained Release of Growth Factor for Chronic Wound Healing (2023-03-20)
期刊：Advanced Healthcare Materials
影响因子 ：10.0
引用产品： NIH/3T3 细胞
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Electroactive and antibacterial wound dressings based on Ti3C2Tx MXene/poly(ε-caprolactone)/gelatin coaxial electrospun nanofibrous membranes (2023-05-31)
期刊：Nano Research
影响因子 ：9.9
引用产品： NIH/3T3 细胞
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Sacrificial scaffold-assisted direct ink writing of engineered aortic valve prostheses (2023-08-14)
期刊：Biofabrication
影响因子 ：9.0
引用产品： NIH/3T3 细胞
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Enhanced single RNA imaging reveals dynamic gene expression in live animals (2023-03-03)
期刊：eLife
影响因子 ：7.7
引用产品： 293T/17 细胞 , NIH/3T3 细胞
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Stromal and tumor immune microenvironment reprogramming through multifunctional cisplatin-based liposomes boosts the efficacy of anti-PD-1 immunotherapy in pancreatic cancer (2023-11-03)
期刊：Biomaterials Science
影响因子 ：6.6
引用产品： NIH/3T3 细胞 , 特级胎牛血清
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Effect of manganese ion doping on magnetic properties and magnetic induction heating of Zn-Al ferrite nanoparticles (2023-07-31)
期刊：JOURNAL OF ALLOYS AND COMPOUNDS
DOI：10.1016/j.jallcom.2023.171597
影响因子 ：6.2
引用产品： 293T [HEK-293T] 细胞 , C2C12 细胞 , NIH/3T3 细胞
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Nano-baicalein facilitates chemotherapy in breast cancer by targeting tumor microenvironment (2023-02-24)
期刊：INTERNATIONAL JOURNAL OF PHARMACEUTICS
DOI：10.1016/j.ijpharm.2023.122778
影响因子 ：5.8
引用产品： NIH/3T3 细胞 , 4T1 细胞
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Multi-targeting liposomal codelivery of cisplatin and rapamycin inhibits pancreatic cancer growth and metastasis through stromal modulation (2023-08-14)
期刊：INTERNATIONAL JOURNAL OF PHARMACEUTICS
DOI：10.1016/j.ijpharm.2023.123316
影响因子 ：5.8
引用产品： 特级胎牛血清 , NIH/3T3 细胞
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Peficitinib ameliorates doxorubicin-induced cardiotoxicity by suppressing cellular senescence and enhances its antitumor activity (2023-07-12)
期刊：INTERNATIONAL IMMUNOPHARMACOLOGY
DOI：10.1016/j.intimp.2023.110630
影响因子 ：5.6
引用产品： NIH/3T3 细胞 , A549 [A-549] 细胞 , MDA-MB-231 细胞 , H9c2 (2-1) 细胞
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Gracillin relieves pulmonary fibrosis by suppressing the STAT3 axis (2023-05-29)
期刊：JOURNAL OF ETHNOPHARMACOLOGY
影响因子 ：5.4
引用产品： NIH/3T3 细胞
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Two-Pore-Domain Potassium Channel TREK–1 Mediates Pulmonary Fibrosis through Macrophage M2 Polarization and by Direct Promotion of Fibroblast Differentiation (2023-04-26)
期刊：Biomedicines
DOI：10.3390/biomedicines11051279
影响因子 ：4.7
引用产品： NIH/3T3 细胞 , RAW 264.7 细胞 , DMEM高糖 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗)，100×
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Imperatorin derivative OW1, a new vasoactive compound, attenuates cell proliferation and migration by inhibiting Nox1-mediated oxidative stress (2023-02-15)
期刊：JOURNAL OF PHARMACY AND PHARMACOLOGY
影响因子 ：3.3
引用产品： NIH/3T3 细胞
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Long Non-Coding RNA Dancr Affects Myocardial Fibrosis in Atrial Fibrillation Mice via the MicroRNA-146b-5p/Smad5 Axis (2023-11-01)
期刊：Acta Cardiologica Sinica
DOI：10.6515/ACS.202311_39(6).20230619B
影响因子 ：1.9
引用产品： HL-1 细胞 , NIH/3T3 细胞
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Antibacterial HPL-DOSS surface coating for commercial plastics (2023-09-11)
期刊：JOURNAL OF THE CHINESE CHEMICAL SOCIETY
影响因子 ：1.8
引用产品： NIH/3T3 细胞
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Ultrasound-triggered piezocatalytic composite hydrogels for promoting bacterial-infected wound healing (2022-12-14)
期刊：Bioactive Materials
DOI：10.1016/j.bioactmat.2022.11.023
影响因子 ：16.9
引用产品： NIH/3T3 细胞
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Inert Pepper aptamer-mediated endogenous mRNA recognition and imaging in living cells (2008-02-20)
期刊：NUCLEIC ACIDS RESEARCH
影响因子 ：14.9
引用产品： NIH/3T3 细胞 , NIH/3T3细胞专用培养基
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Scaffold-Free Tracheal Engineering via a Modular Strategy Based on Cartilage and Epithelium Sheets (2022-12-02)
期刊：Advanced Healthcare Materials
影响因子 ：11.1
引用产品： NIH/3T3 细胞
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Nanoparticle-Stabilized Emulsion Bioink for Digital Light Processing Based 3D Bioprinting of Porous Tissue Constructs (2003-02-20)
期刊：Advanced Healthcare Materials
影响因子 ：11.1
引用产品： NIH/3T3 细胞 , C2C12 细胞 , SK-Hep-1 细胞
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Facile fabrication of chitin/ZnO composite hydrogels for infected wound healing (2022-08-17)
期刊：Biomaterials Science
影响因子 ：7.6
引用产品： NIH/3T3 细胞 , 人脐静脉内皮细胞
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MerTK-mediated efferocytosis promotes immune tolerance and tumor progression in osteosarcoma through enhancing M2 polarization and PD-L1 expression (2001-02-20)
期刊：OncoImmunology
DOI：10.1080/2162402X.2021.2024941
影响因子 ：7.2
引用产品： RAW 264.7 细胞 , K7M2 wt [K7M2-WT] 细胞 , NIH/3T3 细胞 , 293T [HEK-293T] 细胞
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The miR-21-5p enriched in the apoptotic bodies of M2 macrophage-derived extracellular vesicles alleviates osteoarthritis by changing macrophage phenotype (2022-10-05)
期刊：Genes & Diseases
DOI：10.1016/j.gendis.2022.09.010
影响因子 ：7.2
引用产品： NIH/3T3 细胞 , 人脐静脉内皮细胞
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A confined crosslinking strategy towards an intelligent organosilica–micellar hybrid drug delivery system (2001-02-20)
期刊：Biomaterials Science
影响因子 ：6.6
引用产品： SMMC-7721 细胞 , NIH/3T3 细胞 , 3T3-L1 细胞 , Hela 细胞
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Structural, physicochemical properties and function of swim bladder collagen in promoting fibroblasts viability and collagen synthesis (2022-12-09)
期刊：LWT-FOOD SCIENCE AND TECHNOLOGY
影响因子 ：6.1
引用产品： NIH/3T3 细胞 , NCTC clone 929 [L 细胞, L-929] 细胞 , DMEM高糖 培养基
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Allyl phenyl selenides as H2O2 acceptors to develop ROS-responsive theranostic prodrugs (2022-09-17)
期刊：BIOORGANIC CHEMISTRY
DOI：10.1016/j.bioorg.2022.106154
影响因子 ：5.3
引用产品： NIH/3T3 细胞
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Differential Foreign Body Reactions between Branched and Linear Glucomannan Scaffolds (2022-12-11)
期刊：Journal of Functional Biomaterials
影响因子 ：4.9
引用产品： NIH/3T3 细胞
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Engineering Antibacterial Activities and Biocompatibility of Hyperbranched Lysine-based Random Copolymers (2022-10-20)
期刊：CHINESE JOURNAL OF POLYMER SCIENCE
影响因子 ：3.8
引用产品： NIH/3T3 细胞
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Study on properties and biocompatibility of poly (butylene succinate) and sodium alginate biodegradable composites for biomedical applications (2022-08-26)
期刊：Materials Research Express
影响因子 ：2.0
引用产品： NIH/3T3 细胞 , BALB/3T3 clone A31 细胞
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Triple Enzyme-Regulated Molecular Hydrogels for Carrier-Free Delivery of Lonidamine (2007-01-20)
期刊：Advanced Functional Materials
影响因子 ：19.0
引用产品： Hacat 细胞 , NIH/3T3 细胞
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Mussel-inspired biocompatible polydopamine/carboxymethyl cellulose/polyacrylic acid adhesive hydrogels with UV-shielding capacity (2001-01-20)
期刊：Cellulose
DOI：10.1007/s10570-020-03596-7
影响因子 ：5.7
引用产品： NIH/3T3 细胞
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Triggering Drug Release and Thermal-Disrupting Interface Induced Mitigation of Composite Photothermal Hydrogel Treating Infectious Wounds (2021-12-13)
期刊：Frontiers in Bioengineering and Biotechnology
影响因子 ：5.7
引用产品： NIH/3T3 细胞
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Danshensu alleviates bleomycin-induced pulmonary fibrosis by inhibiting lung fibroblast-to-myofibroblast transition via the MEK/ERK signaling pathway (2006-01-20)
期刊：Bioengineered
DOI：10.1080/21655979.2021.1944020
影响因子 ：4.9
引用产品： NIH/3T3 细胞
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Adjusting the accuracy of PEGDA-GelMA vascular network by dark pigments via digital light processing printing: (2011-01-20)
期刊：Journal Of Biomaterials Applications
影响因子 ：2.9
引用产品： NIH/3T3 细胞
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Eco-friendly and degradable red phosphorus nanoparticles for rapid microbial sterilization under visible light (2007-00-20)
期刊：Journal of Materials Science & Technology
DOI：10.1016/j.jmst.2020.04.084
影响因子 ：10.9
引用产品： NIH/3T3 细胞
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Genetically engineered bi-functional silk material with improved cell proliferation and anti-inflammatory activity for medical application (2012-08-20)
期刊：Acta Biomaterialia
DOI：10.1016/j.actbio.2018.12.036
影响因子 ：9.3
引用产品： NIH/3T3 细胞 , RAW 264.7 细胞
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microRNA‐542‐5p protects against acute lung injury in mice with severe acute pancreatitis by suppressing the mitogen‐activated protein kinase signaling pathway through the negative regulation of P21‐activated kinase 1 (2009-08-20)
期刊：Journal Of Cellular Biochemistry
影响因子 ：4.0
引用产品： NIH/3T3 细胞
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Adipose-derived mesenchymal stem cells employed exosomes to attenuate AKI-CKD transition through tubular epithelial cell dependent Sox9 activation (2008-07-20)
期刊：Oncotarget
影响因子 ：0.0
引用产品： NIH/3T3 细胞
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