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产品概述
Dulbecco's 改良培养基--DMEM（Dulbecco's Modified Eagle Medium）是在MEM培养基的基础上研制的，与MEM培养基相比，氨基酸的含量增加了2倍，维生素增加了4倍，同时还增加了非必须氨基酸、微量铁离子以及丙酮酸钠。DMEM培养基最初设计葡萄糖含量为1000 mg/L（低糖型），后来又发展出葡萄糖含量为4500 mg/L（高糖型），现已广泛应用于各种细胞的培养。
DMEM高糖型培养基普遍应用于生长快、粘附性低的细胞、杂交瘤的骨髓瘤细胞、克隆细胞、DNA转染的转化细胞、各种原代病毒宿主细胞、单一细胞的培养以及疫苗的生产，例如利用CHO细胞表达EPO和生产乙肝疫苗。
L-谷氨酰胺（Glutamine）是细胞培养中所必需的一种营养元素，但其在溶液中不稳定，会自发降解，不含L-谷氨酰胺的培养基，可以根据研究需要任意调整L-谷氨酰胺的用量，在使用时添加新鲜的L-谷氨酰胺或其替代物更利于细胞的生长。
本产品含有多类细胞培养所需的氨基酸、维生素、无机盐等多种成分，但不含蛋白质、脂类或任何生长因子，故此产品需搭配血清或无血清添加物使用。
成分说明
形态 | 液体 |
浓度 | 1× |
规格 | 500mL |
pH | 7.2-7.4 |
L-谷氨酰胺 | 无 |
NaHCO3 | 3700 mg/L |
D-葡萄糖 | 4500 mg/L |
丙酮酸钠 | 1 mM |
HEPES缓冲剂 | 无 |
酚红指示剂 | 15 mg/L |
储存条件 | 2-8℃，避光 |
运输条件 | 常温 |
有效期 | 18个月 |
注意事项
1、本产品经0.1 μm过滤除菌，使用时应注意无菌操作，避免污染；
2、为保持本产品的最佳使用效果，请勿进行冻融处理；
3、本产品仅用于科研或进一步研究使用，不用于诊断和治疗。
参考文献
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Blow-Spun Si3N4-Incorporated Nanofibrous Dressing with Antibacterial, Anti-Inflammatory, and Angiogenic Activities for Chronic Wound Treatment (2024-02-13)
作者：Pengchao Ma: 1.State Key Laboratory of New Ceramics and Fine Processing, Key Laboratory of Advanced Materials, School of Materials Science and Engineering, Tsinghua University, Bei**g, 100084, China; Chun-Yi Yang: 1.State Key Laboratory of New Ceramics and Fine Processing, Key Laboratory of Advanced Materials, School of Materials Science and Engineering, Tsinghua University, Bei**g, 100084, China; Chengli Li: 1.State Key Laboratory of New Ceramics and Fine Processing, Key Laboratory of Advanced Materials, School of Materials Science and Engineering, Tsinghua University, Bei**g, 100084, China;2.Department of Orthopedics, School of Clinical Medicine, Bei**g Tsinghua Changgung Hospital, Tsinghua University, Bei**g, 102218, China; Peilun Hu: 1.State Key Laboratory of New Ceramics and Fine Processing, Key Laboratory of Advanced Materials, School of Materials Science and Engineering, Tsinghua University, Bei**g, 100084, China;2.Department of Orthopedics, School of Clinical Medicine, Bei**g T
期刊：Blow-Spun Si3N4-Incorporated Nanofibrous Dressing with Antibacterial, Anti-Inflammatory, and Angiogenic Activities for Chronic Wound Treatment
DOI：10.1007/s42765-023-00361-w
影响因子 ：16.1
引用产品： RAW 264.7 细胞 , HUVECs cell , DMEM高糖 培养基 , DMEM高糖 (不含L-谷氨酰胺) 培养基 , HUV-EC-C [HUVEC]细胞专用培养基 , 磷酸盐缓冲液 (PBS) 1×
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Hypoxia-inducible factor-2α promotes fibrosis in non-alcoholic fatty liver disease by enhancing glutamine catabolism and inhibiting yes-associated protein phosphorylation in hepatic stellate cells (2024-02-28)
作者：Ranran Yan:1.Qinghai-Utah Joint Key Lab for High-altitude Medicine, Medical College of Qinghai University, Xining, China. ; 2.Research Center for High Altitude Medicine, Medical College of Qinghai University, Xining, China. ; 3.Key Laboratory of High-Altitude Medicine in Qinghai University, Ministry of Education, Xining, China. ; 4.Key Laboratory for Application of High-Altitude Medicine in Qinghai Province, Xining, China. ; Hao Cai:1.Oncology Department, The Fifth People's Hospital of Qinghai Provincial, Xining, China. ; Xiaofeng Zhou:1.Affiliated Hospital of Qinghai University, Xining, China. ; Guodan Bao:1.Qinghai-Utah Joint Key Lab for High-altitude Medicine, Medical College of Qinghai University, Xining, China. ; 2.Research Center for High Altitude Medicine, Medical College of Qinghai University, Xining, China. ; 3.Key Laboratory of High-Altitude Medicine in Qinghai University, Ministry of Education, Xining, China. ; 4.Affiliated Hospital of Qinghai University, Xining, China. ;
期刊：Hypoxia-inducible factor-2α promotes fibrosis in non-alcoholic fatty liver disease by enhancing glutamine catabolism and inhibiting yes-associated protein phosphorylation in hepatic stellate cells
DOI：10.3389/fendo.2024.1344971
影响因子 ：5.2
引用产品： LX-2 细胞 , DMEM高糖 (不含L-谷氨酰胺) 培养基 , DMEM高糖 (不含L-谷氨酰胺) 培养基
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Functional gelatin hydrogel scaffold with degraded-release of glutamine to enhance cellular energy metabolism for cartilage repair (2022-09-09)
作者：Ran Yang:1.State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China; School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei 230026, PR China. ; Xu Zhang:1.State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China. ; Jiahui Liu:1.State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China; School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei 230026, PR China. ; Xiaoyuan Li:1.Faculty of Chemistry, Northeast Normal University, Changchun 130024, PR China. Electronic address: lixy849@nenu.edu.cn. ; Dongfang Zhou:1.NMPA Key Laboratory for Research and Evaluation of Drug Metabolism, Guangdong Provincial Key Laboratory of Ne
期刊：Functional gelatin hydrogel scaffold with degraded-release of glutamine to enhance cellular energy metabolism for cartilage repair
DOI：10.1016/j.ijbiomac.2022.09.039
影响因子 ：8.0
引用产品： DMEM高糖 培养基 , DMEM高糖 (不含L-谷氨酰胺) 培养基
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