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产品概述
L-谷氨酰胺(L-Glutamine)是细胞培养中所必需的一种营养元素,它不仅是细胞的能量来源,还是参与蛋白质合成和核酸代谢的重要物质, L-谷氨酰胺(L-Glutamine)的缺乏会导致细胞生长不良甚至死亡。L-谷氨酰胺(L-Glutamine)在溶液中不稳定,容易自发降解,因此在一段时间之后或细胞生长不良时,需要在培养液中补充一定量的L-谷氨酰胺(L-Glutamine),以满足细胞生长的需要。
产品信息
形态 | 液体 |
规格 | 100mL |
PH | 4.7-6.0 |
溶剂 | 0.85% NaCl溶液 |
L-谷氨酰胺 | 200mM |
推荐工作浓度 | 1 mM-4 mM |
储存条件 | -5~-20℃ |
运输条件 | 低温 |
有效期 | 12个月 |
注意事项
1. 本产品经0.1 μm过滤除菌;
2. 使用本产品时应注意无菌操作,避免污染;
3. 本产品为200 mM浓缩液,请根据需要稀释后使用;
4. 不宜长时间放置于室温环境或2-8℃长期保存;
5. 2-8℃中解冻后溶液变成乳液状属正常现象,该情况系L-谷氨酰胺受温度影响导致溶解度降低而析出,将该溶液表面用75%酒精擦拭摇匀后放入37℃培养箱30分钟-40分钟可以溶解至澄清透明,最后摇匀使用,使用过程中切忌反复冻融,用量较少时建议分装冻存;
6. 本产品仅用于科研或进一步研究使用,不用于诊断和治疗。
参考文献
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The m6A methylation and expression profiles of mouse neural stem cells after hypoxia/reoxygenation (2024-02-16)
作者:Shaoqiong Zhang:1.Department of Anesthesiology, The First Hospital of China Medical University, Shenyang, China. ; Kaile Cui:1.Department of Anesthesiology, The First Hospital of China Medical University, Shenyang, China. ; Yuanyuan Li:1.Department of Anesthesiology, The First Hospital of China Medical University, Shenyang, China. ; Yiting Fan:1.Department of Anesthesiology, The First Hospital of China Medical University, Shenyang, China. ; Dongxu Wang:1.Department of Anesthesiology, The First Hospital of China Medical University, Shenyang, China. ; Xingen Yao:1.Department of Anesthesiology, The First Hospital of China Medical University, Shenyang, China. ; Bo Fang:1.Department of Anesthesiology, The First Hospital of China Medical University, Shenyang, China. bfang@cmu.edu.cn. ;
期刊:The m6A methylation and expression profiles of mouse neural stem cells after hypoxia/reoxygenation
DOI:10.1186/s13287-024-03658-8
影响因子 :7.5
引用产品: DMEM高糖 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗),100× , L-谷氨酰胺溶液 (200mM),100×
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Rapid discrimination between clinical Clostridioides difficile infection and colonization by quantitative detection of TcdB toxin using a real-time cell analysis system (2024-01-23)
作者:Yuhang Shen:1.School of Laboratory Medicine, Hangzhou Medical College, Hangzhou, China. ; 2.Key Laboratory of Biomarkers and In Vitro Diagnosis Translation of Zhejiang Province, Hangzhou, China. ; 3.Institute of Ageing Research, School of Basic Medical Sciences, Hangzhou Normal University, Hangzhou, China. ; Shan Lin:1.School of Laboratory Medicine, Hangzhou Medical College, Hangzhou, China. ; 2.Key Laboratory of Biomarkers and In Vitro Diagnosis Translation of Zhejiang Province, Hangzhou, China. ; 3.TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, China. ; Peijun You:1.School of Laboratory Medicine, Hangzhou Medical College, Hangzhou, China. ; 2.Key Laboratory of Biomarkers and In Vitro Diagnosis Translation of Zhejiang Province, Hangzhou, China. ; Yu Chen:1.School of Laboratory Medicine, Hangzhou Medical College, Hangzhou, China. ; 2.Key Laboratory of Biomarkers and In Vitro Diagnosis Translation of Zhejiang Province, Hangzhou, China. ; Yun Luo
期刊:Rapid discrimination between clinical Clostridioides difficile infection and colonization by quantitative detection of TcdB toxin using a real-time cell analysis system
DOI:10.3389/fmicb.2024.1348892
影响因子 :5.2
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Drug resistance‑related gene targets and molecular mechanisms in the A2780/Taxol‑resistant epithelial ovarian cancer cell line (2024-03-26)
作者:Ruihui Yang:1.Department of Cell Biology and Medical Genetics, School of Basic Medical Sciences, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, P.R. China. ; Huainian Zhang:1.Department of Pathology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China. ; Zexin Chen:1.Department of Cell Biology and Medical Genetics, School of Basic Medical Sciences, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, P.R. China. ; Tao Zhang:1.Department of Laboratory, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, Guangdong 510080, P.R. China. ; Peng Wei:1.Department of Physiology, School of Basic Medical Sciences, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, P.R. China. ; Huaguo Liang:1.Department of Cell Biology and Medical Genetics, School of Basic Medical Sciences, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, P.R. China. ; Yaoyao He:1.Department of Cell Biology and Me
期刊:Drug resistance‑related gene targets and molecular mechanisms in the A2780/Taxol‑resistant epithelial ovarian cancer cell line
影响因子 :2.9
引用产品: L-谷氨酰胺溶液 (200mM),100×
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Role of macrophage AHR/TLR4/STAT3 signaling axis in the colitis induced by non-canonical AHR ligand aflatoxin B1 (2023-03-23)
作者:Liwen Zhang:1.Department of Toxicology and Nutrition, School of Public Health, Cheeloo College of Medicine, Shandong University, 250012, China; Division of Chemistry and Physics, Shandong Center for Disease Control and Prevention, Jinan 250014, China. ; Dong Cheng:1.Division of Toxicology, Shandong Center for Disease Control and Prevention, Jinan 250014, China; Division of Chemistry and Physics, Shandong Center for Disease Control and Prevention, Jinan 250014, China. ; Jing Zhang:1.Division of Toxicology, Shandong Center for Disease Control and Prevention, Jinan 250014, China; Division of Chemistry and Physics, Shandong Center for Disease Control and Prevention, Jinan 250014, China. ; Hui Tang:1.Division of Toxicology, Shandong Center for Disease Control and Prevention, Jinan 250014, China; Division of Chemistry and Physics, Shandong Center for Disease Control and Prevention, Jinan 250014, China. ; Fenghua Li:1.Division of Toxicology, Shandong Center for Disease Control and Prevent
期刊:Role of macrophage AHR/TLR4/STAT3 signaling axis in the colitis induced by non-canonical AHR ligand aflatoxin B1
DOI:10.1016/j.jhazmat.2023.131262
影响因子 :13.6
引用产品: RPMI-1640 培养基 , L-谷氨酰胺溶液 (200mM),100× , β-巯基乙醇 (55mM),1000× , HEPES液 (1M) , MEM非必需氨基酸溶液 (NEAA),100×
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The enhancement in toxic potency of oxidized functionalized polyethylene-microplastics in mice gut and Caco-2 cells (2023-08-06)
作者:Ji Wang:1.School of Life Sciences, Lanzhou University, No. 222 South Tianshui Road, Lanzhou 730000, Gansu Province, China. ; Huanbing Tian:1.School of Life Sciences, Lanzhou University, No. 222 South Tianshui Road, Lanzhou 730000, Gansu Province, China. ; Yongpeng Shi:1.School of Life Sciences, Lanzhou University, No. 222 South Tianshui Road, Lanzhou 730000, Gansu Province, China. ; Ying Yang:1.School of Life Sciences, Lanzhou University, No. 222 South Tianshui Road, Lanzhou 730000, Gansu Province, China. ; Feifei Yu:1.School of Life Sciences, Lanzhou University, No. 222 South Tianshui Road, Lanzhou 730000, Gansu Province, China. ; Hanwen Cao:1.School of Life Sciences, Lanzhou University, No. 222 South Tianshui Road, Lanzhou 730000, Gansu Province, China. ; Lan Gao:1.School of Life Sciences, Lanzhou University, No. 222 South Tianshui Road, Lanzhou 730000, Gansu Province, China. Electronic address: gaolan@lzu.edu.cn. ; Mingxin Liu:1.State Key Laboratory of Applied Organic Chemist
期刊:The enhancement in toxic potency of oxidized functionalized polyethylene-microplastics in mice gut and Caco-2 cells
DOI:10.1016/j.scitotenv.2023.166057
影响因子 :9.8
引用产品: Caco-2 细胞 , MEM (含NEAA) 培养基 , L-谷氨酰胺溶液 (200mM),100×
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EphB1 promotes the differentiation and maturation of dendritic cells in non-small cell lung cancer (2023-12-07)
作者:Yaohuan Xie:1.Hunan Key laboratory of Early Diagnosis and Precise Treatment of Lung Cancer, The Second Xiangya Hospital, Central South University, Changsha, Hunan, 410013, China; Cancer Research Institute, School of Basic Medical Science, Central South University, Changsha, Hunan, China; NHC Key Laboratory of Carcinogenesis and the Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Xiangya Hospital, Central South University, Changsha, Hunan, China. ; Liyang Zhang:1.Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, Hunan, China; Hypothalamic-pituitary Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan, China. ; Lujuan Wang:1.Hunan Key laboratory of Early Diagnosis and Precise Treatment of Lung Cancer, The Second Xiangya Hospital, Central South University, Changsha
期刊:EphB1 promotes the differentiation and maturation of dendritic cells in non-small cell lung cancer
DOI:10.1016/j.canlet.2023.216567
影响因子 :9.7
引用产品: DMEM/F12 培养基 , 丙酮酸钠溶液 (100mM),100× , L-谷氨酰胺溶液 (200mM),100× , β-巯基乙醇 (55mM),1000×
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SPDEF enhances cancer stem cell-like properties and tumorigenesis through directly promoting GALNT7 transcription in luminal breast cancer (2023-08-26)
作者:Jingyuan Li:1.Department of Laboratory Medicine, the Affiliated Hospital of Southwest Medical University, Sichuan, 646000, P. R. China. ; Xue Wan:1.Department of Laboratory Medicine, the Affiliated Hospital of Southwest Medical University, Sichuan, 646000, P. R. China. ; Dan Xie:1.Department of Laboratory Medicine, the Affiliated Hospital of Southwest Medical University, Sichuan, 646000, P. R. China. ; Hui Yuan:1.Department of Pathophysiology, Mudanjiang Medical University, Heilongjiang, 157011, P. R. China. ; Qin Pei:1.Department of Laboratory Medicine, the Affiliated Hospital of Southwest Medical University, Sichuan, 646000, P. R. China. ; Yanan Luo:1.Department of Laboratory Medicine, the Affiliated Hospital of Southwest Medical University, Sichuan, 646000, P. R. China. ; Yiyu Chen:1.Department of Laboratory Medicine, the Affiliated Hospital of Southwest Medical University, Sichuan, 646000, P. R. China. ; Jiawen Xian:1.Department of Laboratory Medicine, the Affiliated Hospita
期刊:SPDEF enhances cancer stem cell-like properties and tumorigenesis through directly promoting GALNT7 transcription in luminal breast cancer
DOI:10.1038/s41419-023-06098-z
影响因子 :9.0
引用产品: MCF 10A细胞专用培养基 , L-谷氨酰胺溶液 (200mM),100×
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Interferon-γ regulates immunosuppression in septic mice by promoting the Warburg effect through the PI3K/AKT/mTOR pathway (2023-07-11)
作者:Xu-Zhe Fu:1.Department of Emergency Medicine, Shengjing Hospital of China Medical University, Shenyang, China. ; Yu Wang:1.Department of Emergency Medicine, Shengjing Hospital of China Medical University, Shenyang, China. wangy8@sj-hospital.org. ;
期刊:Interferon-γ regulates immunosuppression in septic mice by promoting the Warburg effect through the PI3K/AKT/mTOR pathway
DOI:10.1186/s10020-023-00690-x
影响因子 :5.7
引用产品: DC2.4 细胞 , L-谷氨酰胺溶液 (200mM),100× , 青霉素-链霉素溶液 (双抗),100× , MEM非必需氨基酸溶液 (NEAA),100× , β-巯基乙醇 (55mM),1000× , 特级胎牛血清
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Hybrid Electrically Conductive Hydrogels with Local Nerve Growth Factor Release Facilitate Peripheral Nerve Regeneration (2023-11-10)
作者:Weijie Su:1.Neurosurgery Unit, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China. ; Jiakun Xu:1.Neurosurgery Unit, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China. ; Dating Pei:1.Institute of Biological and Medical Engineering, Guangdong Academy of Sciences, Guangzhou 510500, China. ; 2.Guangdong Key Lab of Medical Electronic Instruments and Polymer Material Products, Guangdong Institute of Medical Instruments, Guangzhou 510500, China. ; 3.National Engineering Research Center for Healthcare Devices, Guangzhou 510500, China. ; Xixi Li:1.Neurosurgery Unit, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China. ; Jia Yang:1.Neurosurgery Unit, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China. ; Zhijie Geng:1.Institute of Biological and Medical Engineering, Guangdong Academy of Sciences, Guangzhou 510500, China. ; 2.Guangdong Key Lab of Medical Electronic Instruments and P
期刊:Hybrid Electrically Conductive Hydrogels with Local Nerve Growth Factor Release Facilitate Peripheral Nerve Regeneration
影响因子 :4.7
引用产品: 大鼠背根神经元细胞完全培养基 , L-谷氨酰胺溶液 (200mM),100×
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Transcriptome analysis reveals the impact of NETs activation on airway epithelial cell EMT and inflammation in bronchiolitis obliterans (2023-11-06)
作者:Zhongji Wu:1.The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510000, People's Republic of China. ; Xiaowen Chen:1.The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510000, People's Republic of China. ; Shangzhi Wu:1.The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510000, People's Republic of China. ; Zhenwei Liu:1.The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510000, People's Republic of China. ; Hongwei Li:1.The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510000, People's Republic of China. ; Kailin Mai:1.Guangzhou Medical University, Guangzhou, 510000, People's Republic of China. ; Yinghui Peng:1.The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510000, People's Republic of China. ; Haidi Zhang:1.The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510000, People's Republic of China. ; Xiaodie Zhang:1.Gu
期刊:Transcriptome analysis reveals the impact of NETs activation on airway epithelial cell EMT and inflammation in bronchiolitis obliterans
DOI:10.1038/s41598-023-45617-y
影响因子 :4.6
引用产品: 人小气道上皮细胞 , DMEM高糖 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗),100× , L-谷氨酰胺溶液 (200mM),100×
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E2F1-mediated ectopic expression of PP1A promotes breast cancer progression via activation of YAP1 (2023-02-12)
作者:Xiaochong Deng:1.Department of Breast and Thyroid Surgery, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, 200072, China. ; Kaiyao Hua:1.Department of Breast and Thyroid Surgery, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, 200072, China. ; Amik Munankarmy:1.Laboratory Medicine & Pathology, Mayo Clinic, Rochester 55902, MN, USA. ; Qifeng Luo:1.Department of Breast and Thyroid Surgery, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, 200072, China. ; Xuehui Wang:1.Department of Breast and Thyroid Surgery, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, 200072, China. ; Lin Fang:1.Department of Breast and Thyroid Surgery, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, 200072, China. Electronic address: fanglin2017@126.com. ;
期刊:E2F1-mediated ectopic expression of PP1A promotes breast cancer progression via activation of YAP1
DOI:10.1016/j.biocel.2023.106389
影响因子 :4.0
引用产品: DMEM/F12 培养基 , 马血清 , MEM非必需氨基酸溶液 (NEAA),100× , 青霉素-链霉素溶液 (双抗),100× , MEM (含NEAA) 培养基 , 特级胎牛血清 , RPMI-1640 培养基 , L-谷氨酰胺溶液 (200mM),100× , DMEM高糖 培养基
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HLA class II antibody activation of endothelial cells induces M2 macrophage differentiation in peripheral blood (2023-01-07)
作者:Yingcong Guo:1.Department of Kidney Transplantation, Nephropathy Hospital, The First Affiliated Hospital of Xi'an Jiaotong University, No. 277 West Yanta Road, Xi'an, 710061, China. ; Bingxuan Zheng:1.Department of Kidney Transplantation, Nephropathy Hospital, The First Affiliated Hospital of Xi'an Jiaotong University, No. 277 West Yanta Road, Xi'an, 710061, China. ; Puxun Tian:1.Department of Kidney Transplantation, Nephropathy Hospital, The First Affiliated Hospital of Xi'an Jiaotong University, No. 277 West Yanta Road, Xi'an, 710061, China. ; 2.Institute of Organ Transplantation, Xi'an Jiaotong University, Xi'an, 710061, China. ; Jin Zheng:1.Department of Kidney Transplantation, Nephropathy Hospital, The First Affiliated Hospital of Xi'an Jiaotong University, No. 277 West Yanta Road, Xi'an, 710061, China. ; 2.Institute of Organ Transplantation, Xi'an Jiaotong University, Xi'an, 710061, China. ; Yang Li:1.Department of Kidney Transplantation, Nephropathy Hospital, The First Affil
期刊:HLA class II antibody activation of endothelial cells induces M2 macrophage differentiation in peripheral blood
DOI:10.1007/s10157-022-02307-9
影响因子 :2.3
引用产品: MCDB 131 (不含L-谷氨酰胺) 培养基 , L-谷氨酰胺溶液 (200mM),100× , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗),100×
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Insights into an NEk2 inhibitory profile of nitidine chloride by molecular docking and biological evaluation (2022-10-09)
作者:Danni Li:1.School of Chemistry and Chemical Engineering, Guangxi Key Laboratory for Polysaccharide Materials and Modifications, Guangxi Minzu University, No.158, Da Xue Xi street, Xixiangtang District, Nanning, 530006, Guangxi, China. lidanni@gxmzu.edu.cn. ; Jiahao Lu:1.School of Chemistry and Chemical Engineering, Guangxi Key Laboratory for Polysaccharide Materials and Modifications, Guangxi Minzu University, No.158, Da Xue Xi street, Xixiangtang District, Nanning, 530006, Guangxi, China. ; Qiying Zhang:1.School of Chemistry and Chemical Engineering, Guangxi Key Laboratory for Polysaccharide Materials and Modifications, Guangxi Minzu University, No.158, Da Xue Xi street, Xixiangtang District, Nanning, 530006, Guangxi, China. ; Yuzhu Zhou:1.School of Chemistry and Chemical Engineering, Guangxi Key Laboratory for Polysaccharide Materials and Modifications, Guangxi Minzu University, No.158, Da Xue Xi street, Xixiangtang District, Nanning, 530006, Guangxi, China. ; Long Li:1.School of
期刊:Insights into an NEk2 inhibitory profile of nitidine chloride by molecular docking and biological evaluation
DOI:10.1186/s13065-022-00870-6
影响因子 :4.1
引用产品: DMEM/F12 培养基 , 胰岛素-转铁蛋白-硒添加剂 (ITS-G),100× , L-谷氨酰胺溶液 (200mM),100×
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LncRNA MALAT1 Targets miR-9-3p to Upregulate SAP97 in the Hippocampus of Mice with Vascular Dementia (2022-10-13)
作者:Pengwei Wang:1.Department of Neurology, The 2nd Affiliated Hospital of Harbin Medical University, No. 246, Xuefu Road, Harbin, 150001, Heilongjiang, China. ; Senlin Mao:1.Department of Neurology, The 2nd Affiliated Hospital of Harbin Medical University, No. 246, Xuefu Road, Harbin, 150001, Heilongjiang, China. ; Tingting Yi:1.Department of Neurology, The 2nd Affiliated Hospital of Harbin Medical University, No. 246, Xuefu Road, Harbin, 150001, Heilongjiang, China. ; Lihua Wang:1.Department of Neurology, The 2nd Affiliated Hospital of Harbin Medical University, No. 246, Xuefu Road, Harbin, 150001, Heilongjiang, China. wlh_hrbmush@sina.com. ;
期刊:LncRNA MALAT1 Targets miR-9-3p to Upregulate SAP97 in the Hippocampus of Mice with Vascular Dementia
DOI:10.1007/s10528-022-10289-2
影响因子 :2.2
引用产品: L-谷氨酰胺溶液 (200mM),100×
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