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产品概述
L-谷氨酰胺(L-Glutamine)是细胞培养中所必需的一种营养元素,它不仅是细胞的能量来源,还是参与蛋白质合成和核酸代谢的重要物质, L-谷氨酰胺(L-Glutamine)的缺乏会导致细胞生长不良甚至死亡。L-谷氨酰胺(L-Glutamine)在溶液中不稳定,容易自发降解,因此在一段时间之后或细胞生长不良时,需要在培养液中补充一定量的L-谷氨酰胺(L-Glutamine),以满足细胞生长的需要。
产品信息
形态 | 液体 |
规格 | 100mL |
PH | 4.7-6.0 |
溶剂 | 0.85% NaCl溶液 |
L-谷氨酰胺 | 200mM |
推荐工作浓度 | 1 mM-4 mM |
储存条件 | -5~-20℃ |
运输条件 | 低温 |
有效期 | 12个月 |
注意事项
1. 本产品经0.1 μm过滤除菌;
2. 使用本产品时应注意无菌操作,避免污染;
3. 本产品为200 mM浓缩液,请根据需要稀释后使用;
4. 不宜长时间放置于室温环境或2-8℃长期保存;
5. 2-8℃中解冻后溶液变成乳液状属正常现象,该情况系L-谷氨酰胺受温度影响导致溶解度降低而析出,将该溶液表面用75%酒精擦拭摇匀后放入37℃培养箱30分钟-40分钟可以溶解至澄清透明,最后摇匀使用,使用过程中切忌反复冻融,用量较少时建议分装冻存;
6. 本产品仅用于科研或进一步研究使用,不用于诊断和治疗。
参考文献
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Glutamine-glutamate centered metabolism as the potential therapeutic target against Japanese encephalitis virus-induced encephalitis (2025-01-22)
期刊:Cell and Bioscience
DOI:10.1186/s13578-024-01340-3
影响因子 :6.1
引用产品: DMEM高糖(不含L-谷氨酰胺) 培养基 , DMEM高糖 培养基 , 青霉素-链霉素溶液(双抗),100× , MEM(含NEAA) 培养基 , L-谷氨酰胺溶液(200 mM),100× , 磷酸盐缓冲液(PBS) 1×
-
Identification of critical biomarkers and immune landscape patterns in glioma based on multi-database (2025-01-13)
期刊:Discover Oncology
DOI:10.1007/s12672-024-01653-2
影响因子 :2.8
引用产品: L-谷氨酰胺溶液(200 mM),100×
-
Yap methylation-induced FGL1 expression suppresses anti-tumor immunity and promotes tumor progression in KRAS-driven lung adenocarcinoma (2024-09-28)
期刊:Cancer Communications
影响因子 :20.1
引用产品: L-谷氨酰胺溶液(200 mM),100×
-
Spatiotemporal-controllable ROS-responsive camptothecin nano-bomb for chemo/photo/immunotherapy in triple-negative breast cancer (2024-12-27)
期刊:JOURNAL OF NANOBIOTECHNOLOGY
DOI:10.1186/s12951-024-03050-x
影响因子 :10.6
引用产品: 4T1 细胞 , 丙酮酸钠溶液(100 mM),100× , L-谷氨酰胺溶液(200 mM),100×
-
Biomimetic Nano-regulator that Induces Cuproptosis and Lactate-Depletion Mediated ROS Storm for Metalloimmunotherapy of Clear Cell Renal Cell Carcinoma (2024-06-10)
期刊:Advanced Healthcare Materials
影响因子 :10.0
引用产品: Renca [RenCa] 细胞 , RPMI-1640 培养基 , MEM非必需氨基酸溶液(NEAA),100× , 丙酮酸钠溶液(100 mM),100× , L-谷氨酰胺溶液(200 mM),100×
-
The m6A methylation and expression profiles of mouse neural stem cells after hypoxia/reoxygenation (2024-02-16)
期刊:Stem Cell Research & Therapy
DOI:10.1186/s13287-024-03658-8
影响因子 :7.5
引用产品: DMEM高糖 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗),100× , L-谷氨酰胺溶液 (200mM),100×
-
Revealing transport, uptake and damage of polystyrene microplastics using a gut-liver-on-a-chip (2024-11-26)
期刊:LAB ON A CHIP
影响因子 :6.1
引用产品: L-谷氨酰胺溶液(200 mM),100×
-
Rapid discrimination between clinical Clostridioides difficile infection and colonization by quantitative detection of TcdB toxin using a real-time cell analysis system (2024-01-23)
期刊:Frontiers in Microbiology
DOI:10.3389/fmicb.2024.1348892
影响因子 :5.2
-
Rituximab potentially improves clinical outcomes of CAR-T therapy for r/r B-ALL via sensitizing leukemia cells to CAR-T-mediated cytotoxicity and reducing CAR-T exhaustion (2024-04-25)
期刊:CELLULAR ONCOLOGY
DOI:10.1007/s13402-024-00945-7
影响因子 :4.9
引用产品: RPMI-1640 培养基 , L-谷氨酰胺溶液(200 mM),100× , 青霉素-链霉素溶液(双抗),100×
-
Pyroptosis mediated by Parkin-NLRP3 negative feedback loop contributed to Parkinson’s disease induced by rotenone (2024-11-15)
期刊:INTERNATIONAL IMMUNOPHARMACOLOGY
DOI:10.1016/j.intimp.2024.113608
影响因子 :4.8
引用产品: L-谷氨酰胺溶液(200 mM),100×
-
Ethnopharmacological study on Adenosma buchneroides Bonati inhibiting inflammation via the regulation of TLR4/MyD88/NF-κB signaling pathway (2024-06-04)
期刊:Natural Products and Bioprospecting
DOI:10.1007/s13659-024-00458-8
影响因子 :4.8
引用产品: DMEM高糖 培养基 , L-谷氨酰胺溶液(200 mM),100× , 青霉素-链霉素溶液(双抗),100× , RAW 264.7 细胞 , 特级胎牛血清
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CXCL3/TGF-β-Mediated Crosstalk Between CAFs and Tumor Cells Augments RCC Progression and Sunitinib Resistance (2024-06-07)
期刊:iScience
DOI:10.1016/j.isci.2024.110224
影响因子 :4.6
引用产品: L-谷氨酰胺溶液(200 mM),100× , RPMI-1640 培养基
-
Down-regulation of ESRP2 inhibits breast cancer cell proliferation via inhibiting cyclinD1 (2024-11-18)
期刊:Scientific Reports
DOI:10.1038/s41598-024-77980-9
影响因子 :3.8
引用产品: L-谷氨酰胺溶液(200 mM),100×
-
Drug resistance?related gene targets and molecular mechanisms in the A2780/Taxol?resistant epithelial ovarian cancer cell line (2024-03-26)
期刊:Oncology Letters
影响因子 :2.9
引用产品: L-谷氨酰胺溶液 (200mM),100×
-
Lepidium meyenii (Maca) polysaccharides mitigate liver toxicity of aflatoxin B1 through activation of NRF-2/GPX and AhR/STAT3 signaling pathways (2024-10-03)
期刊:TOXICON
DOI:10.1016/j.toxicon.2024.108117
影响因子 :2.6
引用产品: THP-1 细胞 , Hank's平衡盐溶液(HBSS),含钙、镁,不含酚红 , PC-12 (High differentiation) 细胞 , 0.25%胰蛋白酶溶液(含EDTA,溶于PBS) , L-谷氨酰胺溶液(200 mM),100× , 特级胎牛血清 , DMEM高糖 培养基
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Role of macrophage AHR/TLR4/STAT3 signaling axis in the colitis induced by non-canonical AHR ligand aflatoxin B1 (2023-03-23)
期刊:JOURNAL OF HAZARDOUS MATERIALS
DOI:10.1016/j.jhazmat.2023.131262
影响因子 :13.6
引用产品: RPMI-1640 培养基 , L-谷氨酰胺溶液 (200mM),100× , β-巯基乙醇 (55mM),1000× , HEPES液 (1M) , MEM非必需氨基酸溶液 (NEAA),100×
-
The enhancement in toxic potency of oxidized functionalized polyethylene-microplastics in mice gut and Caco-2 cells (2023-08-06)
期刊:SCIENCE OF THE TOTAL ENVIRONMENT
DOI:10.1016/j.scitotenv.2023.166057
影响因子 :9.8
引用产品: Caco-2 细胞 , MEM (含NEAA) 培养基 , L-谷氨酰胺溶液 (200mM),100×
-
EphB1 promotes the differentiation and maturation of dendritic cells in non-small cell lung cancer (2023-12-07)
期刊:CANCER LETTERS
DOI:10.1016/j.canlet.2023.216567
影响因子 :9.7
引用产品: DMEM/F12 培养基 , 丙酮酸钠溶液 (100mM),100× , L-谷氨酰胺溶液 (200mM),100× , β-巯基乙醇 (55mM),1000×
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SPDEF enhances cancer stem cell-like properties and tumorigenesis through directly promoting GALNT7 transcription in luminal breast cancer (2023-08-26)
期刊:Cell Death & Disease
DOI:10.1038/s41419-023-06098-z
影响因子 :9.0
引用产品: MCF 10A细胞专用培养基 , L-谷氨酰胺溶液 (200mM),100×
-
Interferon-γ regulates immunosuppression in septic mice by promoting the Warburg effect through the PI3K/AKT/mTOR pathway (2023-07-11)
期刊:MOLECULAR MEDICINE
DOI:10.1186/s10020-023-00690-x
影响因子 :5.7
引用产品: DC2.4 细胞 , L-谷氨酰胺溶液 (200mM),100× , 青霉素-链霉素溶液 (双抗),100× , MEM非必需氨基酸溶液 (NEAA),100× , β-巯基乙醇 (55mM),1000× , 特级胎牛血清
-
The bexarotene derivative OAB-14 ameliorates cognitive decline in APP/PS1 transgenic mice by suppressing microglia-mediated neuroinflammation through the PPAR-γ pathway (2023-09-09)
期刊:INTERNATIONAL IMMUNOPHARMACOLOGY
DOI:10.1016/j.intimp.2023.110911
影响因子 :5.6
引用产品: 特级胎牛血清 , L-谷氨酰胺溶液(200 mM),100× , 青霉素-链霉素溶液(双抗),100× , MEM非必需氨基酸溶液(NEAA),100×
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Hybrid Electrically Conductive Hydrogels with Local Nerve Growth Factor Release Facilitate Peripheral Nerve Regeneration (2023-11-10)
期刊:ACS Applied Bio Materials
影响因子 :4.7
引用产品: 大鼠背根神经元细胞完全培养基 , L-谷氨酰胺溶液 (200mM),100×
-
Transcriptome analysis reveals the impact of NETs activation on airway epithelial cell EMT and inflammation in bronchiolitis obliterans (2023-11-06)
期刊:Scientific Reports
DOI:10.1038/s41598-023-45617-y
影响因子 :4.6
引用产品: 人小气道上皮细胞 , DMEM高糖 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗),100× , L-谷氨酰胺溶液 (200mM),100×
-
A lysosome-targeted triazole near-infrared cyanine fluorescent probe for in vivo long-term cell tracking (2023-08-30)
期刊:ANALYST
影响因子 :4.2
引用产品: 特级胎牛血清 , L-谷氨酰胺溶液(200 mM),100×
-
E2F1-mediated ectopic expression of PP1A promotes breast cancer progression via activation of YAP1 (2023-02-12)
期刊:INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
DOI:10.1016/j.biocel.2023.106389
影响因子 :4.0
引用产品: DMEM/F12 培养基 , 马血清 , MEM非必需氨基酸溶液 (NEAA),100× , 青霉素-链霉素溶液 (双抗),100× , MEM (含NEAA) 培养基 , 特级胎牛血清 , RPMI-1640 培养基 , L-谷氨酰胺溶液 (200mM),100× , DMEM高糖 培养基
-
HLA class II antibody activation of endothelial cells induces M2 macrophage differentiation in peripheral blood (2023-01-07)
期刊:Clinical and Experimental Nephrology
DOI:10.1007/s10157-022-02307-9
影响因子 :2.3
引用产品: MCDB 131 (不含L-谷氨酰胺) 培养基 , L-谷氨酰胺溶液 (200mM),100× , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗),100×
-
Insights into an NEk2 inhibitory profile of nitidine chloride by molecular docking and biological evaluation (2022-10-09)
期刊:BMC Chemistry
DOI:10.1186/s13065-022-00870-6
影响因子 :4.1
引用产品: DMEM/F12 培养基 , 胰岛素-转铁蛋白-硒添加剂 (ITS-G),100× , L-谷氨酰胺溶液 (200mM),100×
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LncRNA MALAT1 Targets miR-9-3p to Upregulate SAP97 in the Hippocampus of Mice with Vascular Dementia (2022-10-13)
期刊:BIOCHEMICAL GENETICS
DOI:10.1007/s10528-022-10289-2
影响因子 :2.2
引用产品: L-谷氨酰胺溶液 (200mM),100×
-
Negative pressure wound therapy improves bone regeneration by promoting osteogenic differentiation via the AMPK-ULK1-autophagy axis (2021-12-29)
期刊:Autophagy
DOI:10.1080/15548627.2021.2016231
影响因子 :16.0
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