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产品概述
MEM培养基（Minimum Essential Medium）也称最低必需培养基、最小基本培养基或低限量Eagle培养基，由Harry Eagle在Eagle基本培养基（BEM）的基础上发展而来的，是一种最基本、适用范围最广的培养基，是动物细胞培养中最常用的培养基之一。MEM培养基仅含有12种必需氨基酸、谷氨酰胺和8种维生素，成分简单，主要用于贴壁细胞的培养，配方修改后也可用于其他类型细胞培养。
MEM含NEAA（非必须氨基酸）的培养基，是在MEM培养基的基础上添加L-丙氨酸、L-谷氨酸、L-天（门）冬酰胺、L-天（门）冬氨酸、L-脯氨酸、L-丝氨酸和甘氨酸7种NEAA，能降低细胞培养时细胞自身生产非必须氨基酸的副作用，有效促进细胞增殖代谢。不含葡萄糖的MEM培养基可以根据研究需要，随意调节葡萄糖的浓度，方便快捷。
本产品含有多类细胞培养所需的氨基酸、维生素、无机盐等多种成分，但不含蛋白质、脂类或任何生长因子，故此产品需搭配血清或无血清添加物使用。
成分说明
形态 | 液体 |
浓度 | 1× |
规格 | 500mL |
pH | 7.2-7.4 |
平衡盐 | Earle’s 盐 |
NEAA | 含 |
L-谷氨酰胺 | 2 mM |
NaHCO3 | 2200 mg/L |
D-葡萄糖 | 无 |
HEPES缓冲剂 | 无 |
酚红指示剂 | 10 mg/L |
储存条件 | 2-8℃，避光 |
运输条件 | 常温 |
有效期 | 18个月 |
注意事项
1、本产品经0.1 μm过滤除菌，使用时应注意无菌操作，避免污染；
2、为保持本产品的最佳使用效果，请勿进行冻融处理；
3、本产品仅用于科研或进一步研究使用，不用于诊断和治疗。
参考文献
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NMDA Receptors Regulate Oxidative Damage in Keratinocytes during Complex Regional Pain Syndrome in HaCaT Cells and Male Rats (2024-02-18)
作者：Bei Wen:1.Department of Anesthesiology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China. ; He Zhu:1.Department of Anesthesiology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China. ; Jijun Xu:1.Department of Pain Management, Cleveland Clinic, Cleveland, OH 44195, USA. ; 2.Department of Inflammation and Immunity, Cleveland Clinic, Cleveland, OH 44195, USA. ; Li Xu:1.Department of Anesthesiology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China. ; Yuguang Huang:1.Department of Anesthesiology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China. ;
期刊：NMDA Receptors Regulate Oxidative Damage in Keratinocytes during Complex Regional Pain Syndrome in HaCaT Cells and Male Rats
影响因子 ：7.0
引用产品： MEM无糖 (含NEAA) 培养基
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Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes Attenuate Oxygen-Glucose Deprivation/Reperfusion-Induced Microglial Pyroptosis by Promoting FOXO3a-Dependent Mitophagy (2011-01-20)
作者：Zhenzhen Hu:1.Clinical Molecular Diagnostic Laboratory, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210003, China. ; Ya Yuan:1.The Second Clinical Medical School of Nanjing Medical University, Nanjing, Jiangsu 210011, China. ; Xiuli Zhang:1.The Second Clinical Medical School of Nanjing Medical University, Nanjing, Jiangsu 210011, China. ; Yifeng Lu:1.The Second Clinical Medical School of Nanjing Medical University, Nanjing, Jiangsu 210011, China. ; Na Dong:1.Children's Health Center, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210003, China. ; Xiuqin Jiang:1.Clinical Molecular Diagnostic Laboratory, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210003, China. ; Jinjin Xu:1.Clinical Molecular Diagnostic Laboratory, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210003, China. ; Datong Zheng:1.Clinical Molecular Diagnostic Laboratory, The Second Af
期刊：Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes Attenuate Oxygen-Glucose Deprivation/Reperfusion-Induced Microglial Pyroptosis by Promoting FOXO3a-Dependent Mitophagy
影响因子 ：0.0
引用产品： MEM无糖 (含NEAA) 培养基
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