产品中心Cell Resources
联系我们CONTACT US
400-999-210024小时服务热线
产品概述
MCDB是为特定细胞设计的低蛋白和无血清培养的培养基，MCDB 131是MCDB培养基的一种，是由Knedler和Ham设计开发出来的，最初用于培养人微血管内皮细胞（HMVEC）。MCDB 131培养基除用于内皮细胞外，还可用于其它细胞类型，如肝细胞、平滑肌细胞、心肌细胞等。
MCDB 131培养基不同于传统培养基之处在于，其含有微量元素、腐胺、腺嘌呤、胸腺嘧啶以及更高浓度的氨基酸和维生素，在使用时常与EGF、氢化可的松、谷氨酰胺以及低浓度血清搭配使用，血清的添加浓度须根据细胞类型而定。
成分说明
形态 | 液体 |
浓度 | 1× |
规格 | 500mL |
pH | 7.2-7.4 |
L-谷氨酰胺 | 无 |
NaHCO3 | 1176 mg/L |
D-葡萄糖 | 1000 mg/L |
丙酮酸钠 | 1 mM |
HEPES缓冲剂 | 无 |
酚红指示剂 | 12.4 mg/L |
储存条件 | 2-8℃，避光 |
运输条件 | 常温 |
有效期 | 18个月 |
注意事项
1、本产品经0.1 μm过滤除菌，使用时应注意无菌操作，避免污染；
2、为保持本产品的最佳使用效果，请勿进行冻融处理；
3、本产品仅用于科研或进一步研究使用，不用于诊断和治疗。
产品手册
参考文献
-
USP11 exacerbates radiation induced pneumonitis by activating endothelial cell inflammatory response via OTUD5-STING signaling (2024-02-15)
作者：Yiting Tang:1.State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, China; Jiangmen Central Hospital, Affiliated Jiangmen Hospital of Sun Yat-sen University, Jiangmen, China. ; Tingya Wang:1.State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, China. ; Liming Gu:1.State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, China. ; Ying Xu:1.State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, China. ; Zhao Yang:1.Department of Respiratory Medicine, Suzhou Science & Technology Town Hospital, Suzhou, China. ; Wei Zhu:1.State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine
期刊：USP11 exacerbates radiation induced pneumonitis by activating endothelial cell inflammatory response via OTUD5-STING signaling
DOI：10.1016/j.ijrobp.2024.01.220
影响因子 ：7.0
引用产品： MCDB 131 (不含L-谷氨酰胺) 培养基
-
HLA class II antibody activation of endothelial cells induces M2 macrophage differentiation in peripheral blood (2023-01-07)
作者：Yingcong Guo:1.Department of Kidney Transplantation, Nephropathy Hospital, The First Affiliated Hospital of Xi'an Jiaotong University, No. 277 West Yanta Road, Xi'an, 710061, China. ; Bingxuan Zheng:1.Department of Kidney Transplantation, Nephropathy Hospital, The First Affiliated Hospital of Xi'an Jiaotong University, No. 277 West Yanta Road, Xi'an, 710061, China. ; Puxun Tian:1.Department of Kidney Transplantation, Nephropathy Hospital, The First Affiliated Hospital of Xi'an Jiaotong University, No. 277 West Yanta Road, Xi'an, 710061, China. ; 2.Institute of Organ Transplantation, Xi'an Jiaotong University, Xi'an, 710061, China. ; Jin Zheng:1.Department of Kidney Transplantation, Nephropathy Hospital, The First Affiliated Hospital of Xi'an Jiaotong University, No. 277 West Yanta Road, Xi'an, 710061, China. ; 2.Institute of Organ Transplantation, Xi'an Jiaotong University, Xi'an, 710061, China. ; Yang Li:1.Department of Kidney Transplantation, Nephropathy Hospital, The First Affil
期刊：HLA class II antibody activation of endothelial cells induces M2 macrophage differentiation in peripheral blood
DOI：10.1007/s10157-022-02307-9
影响因子 ：2.3
引用产品： MCDB 131 (不含L-谷氨酰胺) 培养基 , L-谷氨酰胺溶液 (200mM)，100× , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗)，100×
-
LncRNA SNHG12 alleviates hypertensive vascular endothelial injury through miR‐25‐3p/SIRT6 pathway (2001-01-20)
作者：Wei Qian:1.Department of Cardiology, The First Affiliated Hospital of Nanchang University, Nanchang, China. ; Ze-Qi Zheng:1.Department of Cardiology, The First Affiliated Hospital of Nanchang University, Nanchang, China. ; Jun-Gang Nie:1.Department of Cardiology, The First Affiliated Hospital of Nanchang University, Nanchang, China. ; Li-Juan Liu:1.Department of Cardiology, The First Affiliated Hospital of Nanchang University, Nanchang, China. ; Xiang-Zhu Meng:1.Department of Cardiology, The First Affiliated Hospital of Nanchang University, Nanchang, China. ; Hong Sun:1.Department of Cardiology, The First Affiliated Hospital of Nanchang University, Nanchang, China. ; Feng-Ming Xiao:1.Department of Cardiology, The First Affiliated Hospital of Nanchang University, Nanchang, China. ; Ting Kang:1.Department of Cardiology, The First Affiliated Hospital of Nanchang University, Nanchang, China. ;
期刊：LncRNA SNHG12 alleviates hypertensive vascular endothelial injury through miR‐25‐3p/SIRT6 pathway
影响因子 ：5.5
引用产品： MCDB 131 (不含L-谷氨酰胺) 培养基 , HMEC-1 细胞
-
Targeting USP11 may alleviate radiation-induced pulmonary fibrosis by regulating endothelium tight junction (2011-01-20)
作者：Yiting Tang:1.State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Soochow University, Suzhou, China. ; 2.Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Suzhou, China. ; Qian Yuan:1.State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Soochow University, Suzhou, China. ; 2.Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Suzhou, China. ; Congzhao Zhao:1.State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Soochow University, Suzhou, China. ; 2.Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Suzhou, China. ; Ying Xu:1.State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Soochow University, Suzhou, China. ; 2.Collaborative Innovation
期刊：Targeting USP11 may alleviate radiation-induced pulmonary fibrosis by regulating endothelium tight junction
DOI：10.1080/09553002.2022.1998711
影响因子 ：3.4
引用产品： MCDB 131 (不含L-谷氨酰胺) 培养基 , HUV-EC-C [HUVEC]细胞专用培养基 , PUMC-HUVEC-T1
FAQs
Q：{{item.question}}
A：
产品资料
识别码示意图
