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产品概述
William's E培养基是由William和Gunn设计出来的，主要用于大鼠肝上皮细胞的长期培养，也可用于其他哺乳动物的肝细胞培养。
L-丙氨酰-L-谷氨酰胺（Alanyl-glutamine，Ala-Glu），又名丙氨酰谷氨酰胺、丙谷二肽，是一种高级细胞培养添加剂，可直接替代细胞培养基中的L-谷氨酰胺。L-谷氨酰胺（Glutamine）是细胞培养中所必需的一种营养素，但其在溶液中不稳定，会自发降解生成氨和焦谷氨酸，其中氨对细胞有害；而L-丙氨酰-L-谷氨酰胺在水溶液中十分的稳定，不会自发的降解。细胞利用其机制是：在细胞培养时，细胞会逐渐向培养液中释放一种肽酶，将L-丙氨酰-L-谷氨酰水解成L-丙氨酸和L-谷氨酰胺，而后细胞会将这两种水解产物吸收利用。细胞利用L-丙氨酰-L-谷氨酰的过程与流加培养策略相似，连续的将低浓度水平的L-谷氨酰胺加入到培养液中，从而提高了L-谷氨酰胺的利用率，且不会生成多余的氨，更利于细胞的生长。L-丙氨酰-L-谷氨酰可以代替等摩尔的L-谷氨酰胺，适用于所有的细胞，几乎无需适应，并且可以延长细胞的培养时间，减少传代次数，即节省了时间也节约了金钱。与添加 L-谷氨酰胺的培养基中培养的细胞相比，活性降低得更慢。 延滞期略微延长的原因是肽酶的释放和二肽的消化需要一定的时间。
本产品含有多类细胞培养所需的氨基酸、维生素、无机盐等多种成分，但不含蛋白质、脂类或任何生长因子，故此产品需搭配血清或无血清添加物使用。
成分说明
形态 | 液体 |
浓度 | 1× |
规格 | 500mL |
pH | 7.2-7.4 |
L-丙氨酰-L-谷氨酰胺 | 2 mM |
NaHCO3 | 2200 mg/L |
D-葡萄糖 | 2000 mg/L |
丙酮酸钠 | 25 mg/L |
HEPES缓冲剂 | 无 |
酚红指示剂 | 10 mg/L |
储存条件 | 2-8℃，避光 |
运输条件 | 常温 |
有效期 | 18个月 |
注意事项
1、本产品经0.1 μm过滤除菌，使用时应注意无菌操作，避免污染；
2、为保持本产品的最佳使用效果，请勿进行冻融处理；
3、本产品仅用于科研或进一步研究使用，不用于诊断和治疗。
参考文献
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Establishment and characterization of a highly metastatic hepatocellular carcinoma cell line (2024-01-07)
作者：Jiacheng Huang:1.Department of Hepatobiliary and Pancreatic Surgery, The Fourth Affiliated Hospital, International Institutes of Medicine, Zhejiang University School of Medicine, Yiwu, China. ; 2.Jinan Microecological Biomedicine Shandong Laboratory, Jinan, China. ; 3.Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China. ; 4.School of Pharmacy and Department of Hepatology, the Affiliated Hospital of Hangzhou Normal University, Hangzhou Normal University, Hangzhou, China. ; Mengqing Sun:1.School of Medicine, Zhejiang University, Hangzhou, China. ; Menglan Wang:1.School of Medicine, Zhejiang University, Hangzhou, China. ; Anning Yu:1.Department of Hepatobiliary and Pancreatic Surgery, The Fourth Affiliated Hospital, International Institutes of Medicine, Zhejiang University School of Medicine, Yiwu, China. ; 2.School of Pharmacy and Department of Hepatology, the Affiliated Hospita
期刊：Establishment and characterization of a highly metastatic hepatocellular carcinoma cell line
DOI：10.1080/21655979.2023.2296775
影响因子 ：4.9
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The E3 ubiquitin ligase NEDD4-1 protects against acetaminophen-induced liver injury by targeting VDAC1 for degradation (2023-01-29)
作者：Yiwei Zhu:1.State Key Laboratory for Zoonotic Diseases, Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China. ; Lin Lei:1.State Key Laboratory for Zoonotic Diseases, Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China. ; Xinghui Wang:1.State Key Laboratory for Zoonotic Diseases, Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China. ; Linfang Chen:1.State Key Laboratory for Zoonotic Diseases, Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China. ; Wei Li:1.State Key Laboratory for Zoonotic Diseases, Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China. ; Jinxia Li:1.State Key Laborat
期刊：The E3 ubiquitin ligase NEDD4-1 protects against acetaminophen-induced liver injury by targeting VDAC1 for degradation
DOI：10.1016/j.apsb.2023.01.019
影响因子 ：14.5
引用产品： D-Hank's平衡盐溶液 (D-HBSS)，不含钙、镁，含酚红 , Hank's平衡盐溶液 (HBSS)，含钙、镁，不含酚红 , William's E (含L-丙氨酰-L-谷氨酰胺) 培养基
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Identification of candidate genes associated with skin yellowness in yellow chickens (2023-01-09)
作者：Shizi He:1.Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou, 510642, Guangdong, China; Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, and Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Guangzhou, 510642, Guangdong, China. ; Tuanhui Ren:1.Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou, 510642, Guangdong, China; Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, and Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Guangzhou, 510642, Guangdong, China. ; Wujian Lin:1.Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou, 510642, Guangdong, China; Guangdong Provincial Key Lab of
期刊：Identification of candidate genes associated with skin yellowness in yellow chickens
影响因子 ：4.4
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