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产品概述
平衡盐溶液(Balanced Salt Solution, BSS)也叫生理溶液(Physiological Solution),是集缓冲液的缓冲力、生理盐水的等渗性以及培养液的营养供应特点于一体的,具有维持渗透压、保持PH稳定以及提供简单的营养的作用,可满足体外实验中组织、器官或细胞的生存和代谢的基本需要。一些平衡盐溶液中还添加了少量酚红,用以指示溶液的酸碱度变化。
Hank's平衡盐溶液(Hank's Balanced Salt Solution, HBSS)是细胞分离或培养中常用的磷酸盐缓冲液之一,主要成分包括NaCl、KCl、KH2PO4、Na2HPO4、NaHCO3、CaCl2、MgCl2、MgSO4和葡萄糖。Hank's平衡盐溶液常用于组织块的漂洗、细胞的洗涤、细胞或组织的运输、其他试剂的配制以及作为细胞计数的稀释液等。
产品信息
形态 | 液体 |
浓度 | 1× |
规格 | 500mL |
pH | 7.2-7.4 |
CaCl2(无水) | 140mg/L |
MgCl2•6H2O | 100mg/L |
MgSO4•7H2O | 100mg/L |
NaHCO3 | 350mg/L |
D-葡萄糖 | 1000mg/L |
酚红指示剂 | 无 |
储存条件 | 2-30℃ |
运输条件 | 室温 |
有效期 | 36个月 |
注意事项
1、本产品为即用型,经0.1μm过滤除菌,可直接使用,使用前无需稀释或过滤除菌等任何处理;
2、使用本产品时应注意无菌操作,避免污染;
3、由于Hank's平衡盐溶液含有钙镁离子,易产生少量沉淀,一般温浴后沉淀即可溶解;若沉淀较多且又无法溶解时,请放弃使用;
4、由于Hank's平衡盐溶液NaHCO3含量较低,不能用于5% CO2的环境,若放入CO2培养箱,溶液将迅速变酸,使用时应注意;
5、本产品仅用于科研或进一步研究使用,不用于诊断和治疗。
参考文献
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Curculigoside Attenuates Endoplasmic Reticulum Stress-Induced Epithelial Cell and Fibroblast Senescence by Regulating the SIRT1-P300 Signaling Pathway (2024-03-29)
作者:Weixi Xie:1.Xiangya Nursing School, Central South University, Changsha 410013, China. ; Lang Deng:1.Xiangya Nursing School, Central South University, Changsha 410013, China. ; Rui Qian:1.Xiangya Nursing School, Central South University, Changsha 410013, China. ; Xiaoting Huang:1.Xiangya Nursing School, Central South University, Changsha 410013, China. ; Wei Liu:1.Xiangya Nursing School, Central South University, Changsha 410013, China. ; Siyuan Tang:1.Xiangya Nursing School, Central South University, Changsha 410013, China. ; 2.The School of Nursing, Ningxia Medical University, Yinchuan 750004, China. ;
期刊:Curculigoside Attenuates Endoplasmic Reticulum Stress-Induced Epithelial Cell and Fibroblast Senescence by Regulating the SIRT1-P300 Signaling Pathway
影响因子 :7.0
引用产品: 青霉素-链霉素溶液 (双抗),100× , Hank's平衡盐溶液 (HBSS),含钙、镁,不含酚红 , HEPES液 (1M) , 特级胎牛血清
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A novel synthesized cyclohexane-hydroxytyrosol derivative suppresses ovarian cancer cell growth through inducing ROS and blocking autophagic flux (2024-02-26)
作者:Guanfei Zhang:1.Center for Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Cardiometabolic Innovation Center, Ministry of Education, Department of Cardiology, First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, China. ; Min Wang:1.Center for Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Cardiometabolic Innovation Center, Ministry of Education, Department of Cardiology, First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, China. ; Yilin Gao:1.Center for Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Cardiometabolic Innovation Center, Ministry of Education, Department of Cardiology, First Affiliated Hospital, Xi'an Jiaotong University, Xi'an,
期刊:A novel synthesized cyclohexane-hydroxytyrosol derivative suppresses ovarian cancer cell growth through inducing ROS and blocking autophagic flux
影响因子 :6.6
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Nilotinib in combination with sunitinib renders MCL-1 for degradation and activates autophagy that overcomes sunitinib resistance in renal cell carcinoma (2024-02-23)
作者:Tingyu Liu:1.State Key Laboratory of Oncology in South China, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-sen University Cancer Center, Guangzhou, 510060, China. ; Xin Yue:1.Institute of Precision Medicine, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, 510080, China. yuex5@mail.sysu.edu.cn. ; Xue Chen:1.State Key Laboratory of Oncology in South China, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-sen University Cancer Center, Guangzhou, 510060, China. ; Ru Yan:1.State Key Laboratory of Oncology in South China, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-sen University Cancer Center, Guangzhou, 510060, China. ; Chong Wu:1.State Key Laboratory of Oncology in South China, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-sen University Cancer Center, Guangzhou, 510060, China. ; Yunzhi Li:1.School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, 510006, China. ; Xianzhang
期刊:Nilotinib in combination with sunitinib renders MCL-1 for degradation and activates autophagy that overcomes sunitinib resistance in renal cell carcinoma
DOI:10.1007/s13402-024-00927-9
影响因子 :6.6
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Intestinal Absorption of Nanoparticles to Reduce Oxidative Stress and Vasoconstriction for Treating Diabetic Nephropathy (2024-02-20)
作者:Chenxin Liu:1.School of Pharmaceutical Sciences, Zhengzhou University, 100 Science Road, Zhengzhou 450001, China. ; Mengxue Pang:1.School of Pharmaceutical Sciences, Zhengzhou University, 100 Science Road, Zhengzhou 450001, China. ; Qingyu Wang:1.School of Pharmaceutical Sciences, Zhengzhou University, 100 Science Road, Zhengzhou 450001, China. ; Mei Yan:1.School of Pharmaceutical Sciences, Zhengzhou University, 100 Science Road, Zhengzhou 450001, China. ; Yingying Zhou:1.School of Pharmaceutical Sciences, Zhengzhou University, 100 Science Road, Zhengzhou 450001, China. ; Hanchun Yao:1.School of Pharmaceutical Sciences, Zhengzhou University, 100 Science Road, Zhengzhou 450001, China. ; 2.Key Laboratory of Targeting Therapy and Diagnosis for Critical Diseases, Henan Province, 100 Science Road, Zhengzhou 450001, China. ; Bin Du:1.School of Pharmaceutical Sciences, Zhengzhou University, 100 Science Road, Zhengzhou 450001, China. ; 2.Key Laboratory of Targeting Therapy and Diagnosis
期刊:Intestinal Absorption of Nanoparticles to Reduce Oxidative Stress and Vasoconstriction for Treating Diabetic Nephropathy
DOI:10.1021/acsbiomaterials.3c01353
影响因子 :5.8
引用产品: MEM 培养基 , Hank's平衡盐溶液 (HBSS),含钙、镁,不含酚红
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STAT4 targets KISS1 to inhibit the oxidative damage, inflammation and neuronal apoptosis in experimental PD models by inactivating the MAPK pathway (2024-02-08)
作者:XiaoLei Zhang:1.Department of Neurology, Shanxi People's Hospital, Taiyuan 030012, China. Electronic address: zhangxl2011@yeah.net. ; Yu Wang:1.Department of Neurology, Fifth Hospital of Shanxi Medical University, Taiyuan 030012, China. ; Jia Lv:1.Department of Neurology, Fifth Hospital of Shanxi Medical University, Taiyuan 030012, China. ;
期刊:STAT4 targets KISS1 to inhibit the oxidative damage, inflammation and neuronal apoptosis in experimental PD models by inactivating the MAPK pathway
DOI:10.1016/j.neuint.2024.105683
影响因子 :4.2
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FTO attenuates TNF-α-induced damage of proximal tubular epithelial cells in acute pancreatitis-induced acute kidney injury via targeting AQP3 in an N6-methyladenosine-dependent manner (2024-03-06)
作者:Xinghui Li:1.Department of Radiology, Affiliated Hospital of North Sichuan Medical College, Medical Imaging Key Laboratory of Sichuan Province, Nanchong, Sichuan Province, China. ; Qi Liang:1.Department of Clinical Laboratory, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan Province, China. ; Lu Liu:1.Department of Radiology, Affiliated Hospital of North Sichuan Medical College, Medical Imaging Key Laboratory of Sichuan Province, Nanchong, Sichuan Province, China. ; Shujun Chen:1.Department of Radiology, Affiliated Hospital of North Sichuan Medical College, Medical Imaging Key Laboratory of Sichuan Province, Nanchong, Sichuan Province, China. ; Yong Li:1.Department of Radiology, Affiliated Hospital of North Sichuan Medical College, Medical Imaging Key Laboratory of Sichuan Province, Nanchong, Sichuan Province, China. ; Yu Pu:1.Department of Radiology, Affiliated Hospital of North Sichuan Medical College, Medical Imaging Key Laboratory of Sichuan Province, N
期刊:FTO attenuates TNF-α-induced damage of proximal tubular epithelial cells in acute pancreatitis-induced acute kidney injury via targeting AQP3 in an N6-methyladenosine-dependent manner
DOI:10.1080/0886022X.2024.2322037
影响因子 :3.0
引用产品: HK-2 细胞 , MEM (含NEAA) 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗),100× , Hank's平衡盐溶液 (HBSS),含钙、镁,不含酚红
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The E3 ubiquitin ligase NEDD4-1 protects against acetaminophen-induced liver injury by targeting VDAC1 for degradation (2023-01-29)
作者:Yiwei Zhu:1.State Key Laboratory for Zoonotic Diseases, Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China. ; Lin Lei:1.State Key Laboratory for Zoonotic Diseases, Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China. ; Xinghui Wang:1.State Key Laboratory for Zoonotic Diseases, Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China. ; Linfang Chen:1.State Key Laboratory for Zoonotic Diseases, Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China. ; Wei Li:1.State Key Laboratory for Zoonotic Diseases, Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China. ; Jinxia Li:1.State Key Laborat
期刊:The E3 ubiquitin ligase NEDD4-1 protects against acetaminophen-induced liver injury by targeting VDAC1 for degradation
DOI:10.1016/j.apsb.2023.01.019
影响因子 :14.5
引用产品: D-Hank's平衡盐溶液 (D-HBSS),不含钙、镁,含酚红 , Hank's平衡盐溶液 (HBSS),含钙、镁,不含酚红 , William's E (含L-丙氨酰-L-谷氨酰胺) 培养基
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Amelioration of gamma irradiation-induced salivary gland damage in mice using melatonin (2023-06-30)
作者:Jingming Ren:1.Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China. ; Tong Zhu:1.Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China. ; Zhouxuan Wang:1.Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China. ; Lu Lu:1.Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China. ; Saijun Fan:1.Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Scie
期刊:Amelioration of gamma irradiation-induced salivary gland damage in mice using melatonin
影响因子 :10.3
引用产品: Hank's平衡盐溶液 (HBSS),含钙、镁,不含酚红 , FaDu 细胞
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Preparation and characterization of a novel composite acellular matrix/hyaluronic acid thermosensitive hydrogel for interstitial cystitis/bladder pain syndrome (2023-11-16)
作者:Wei Guo:1.Department of Urology, Beijing Chao-Yang Hospital, Beijing, China. ; Haichao Liu:1.Department of Urology, Hebei Yanda Hospital, West of SiPuLan Road, Langfang, China. ; Jiaxing Zhang:1.Department of Urology, Hebei Yanda Hospital, West of SiPuLan Road, Langfang, China. ; Jianzhong Zhang:1.Department of Urology, Beijing Chao-Yang Hospital, Beijing, China. ; Fei Wang:1.Department of Urology, Beijing Chao-Yang Hospital, Beijing, China. ; Peng Zhang:1.Department of Urology, Beijing Chao-Yang Hospital, Beijing, China. ; Yunbo Yang:1.Department of Urology, Hebei Yanda Hospital, West of SiPuLan Road, Langfang, China. ;
期刊:Preparation and characterization of a novel composite acellular matrix/hyaluronic acid thermosensitive hydrogel for interstitial cystitis/bladder pain syndrome
影响因子 :4.9
引用产品: Hank's平衡盐溶液 (HBSS),含钙、镁,不含酚红 , 磷酸盐缓冲液 (PBS) 1× , 小鼠骨髓间充质干细胞
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Overcoming the resistance of hepatocellular carcinoma to PD-1/PD-L1 inhibitor and the resultant immunosuppression by CD38 siRNA-loaded extracellular vesicles (2022-12-26)
作者:Jun Deng:1.Department of General Surgery, the First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 330006, China. ; Hui Ke:1.Surgical Dressing Room, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430022, China. ;
期刊:Overcoming the resistance of hepatocellular carcinoma to PD-1/PD-L1 inhibitor and the resultant immunosuppression by CD38 siRNA-loaded extracellular vesicles
DOI:10.1080/2162402X.2022.2152635
影响因子 :7.7
引用产品: HuH-7 细胞 , HCCLM3 细胞 , MHCC97-L 细胞 , RPMI-1640 培养基 , BNL 1ME A.7R.1 细胞 , DMEM高糖 培养基 , 小鼠骨髓间充质干细胞 , Hank's平衡盐溶液 (HBSS),含钙、镁,不含酚红
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Perfluorooctane Sulfonate Induces Dysfunction of Human Umbilical Vein Endothelial Cells via Ferroptosis Pathway (2022-08-28)
作者:Jiajing Cui:1.Department of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun 130021, China. ; Pingwei Wang:1.Department of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun 130021, China. ; Shuqi Yan:1.Department of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun 130021, China. ; Yujun Liang:1.Department of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun 130021, China. ; Dongge Liu:1.Department of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun 130021, China. ; Shuping Ren:1.Department of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun 130021, China. ;
期刊:Perfluorooctane Sulfonate Induces Dysfunction of Human Umbilical Vein Endothelial Cells via Ferroptosis Pathway
影响因子 :4.5
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Improved mechanical, bio-corrosion properties and in vitro cell responses of Ti-Fe alloys as candidate dental implants (2001-01-20)
作者:Jingzhe Niu:1.State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China. ; Yanhua Guo:1.College of Materials Science and Engineering and Tech Institute for Advanced Materials, Nanjing Tech University, Nanjing, 210009, China. Electronic address: guoyanhua@njtech.edu.cn. ; Kai Li:1.State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China. ; Wenjuan Liu:1.College of Materials Science and Engineering and Tech Institute for Advanced Materials, Nanjing Tech University, Nanjing, 210009, China. ; Zhenhua Dan:1.College of Materials Science and Engineering and Tech Institute for Advanced Materials, Nanjing Tech University, Nanjing, 210009, China. ; Zhonggang Sun:1.College of Materials Science and Engineering and Tech Institute for Advanced Materials, Nanjing Tech University, Nanjing, 210009, China. ; Hui Chang:1.College of Materials Science and Engineering and Tech Institute for Advanced Materi
期刊:Improved mechanical, bio-corrosion properties and in vitro cell responses of Ti-Fe alloys as candidate dental implants
DOI:10.1016/j.msec.2021.111917
影响因子 :7.9
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Pharmacological effects of Pugionium cornutum (L.) Gaertn. extracts on gastrointestinal motility are partially mediated by quercetin (2009-01-20)
作者:Chencan Su:1.Key Laboratory of Synthetic and Natural Functional Molecule of Ministry of Education, College of Chemistry and Materials Science, National Demonstration Center for Experimental Chemistry Education, Northwest University, 229 North Taibai Avenue, Xi'an, 710127, Shaanxi, China. ; Haoyu Li:1.Key Laboratory of Synthetic and Natural Functional Molecule of Ministry of Education, College of Chemistry and Materials Science, National Demonstration Center for Experimental Chemistry Education, Northwest University, 229 North Taibai Avenue, Xi'an, 710127, Shaanxi, China. ; 2.College of Chemistry and Chemical Engineering, Yan'an University, Yan'an, 716000, Shaanxi, China. ; Bang Chen:1.Key Laboratory of Synthetic and Natural Functional Molecule of Ministry of Education, College of Chemistry and Materials Science, National Demonstration Center for Experimental Chemistry Education, Northwest University, 229 North Taibai Avenue, Xi'an, 710127, Shaanxi, China. ; Cong Li:1.Key Laboratory
期刊:Pharmacological effects of Pugionium cornutum (L.) Gaertn. extracts on gastrointestinal motility are partially mediated by quercetin
DOI:10.1186/s12906-021-03395-y
影响因子 :3.9
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MicroRNA-1251-5p Promotes Carcinogenesis and Autophagy via Targeting the Tumor Suppressor TBCC in Ovarian Cancer Cells (2006-09-20)
作者:Yang Shao:1.Cancer Institute, Fudan University Shanghai Cancer Center, Shanghai 200032, China; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China. ; Xiaomin Liu:1.Lab for Noncoding RNA & Cancer, School of Life Sciences, Shanghai University, Shanghai 200444, China. ; Jiao Meng:1.Cancer Institute, Fudan University Shanghai Cancer Center, Shanghai 200032, China; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China. ; Xiaofei Zhang:1.Cancer Institute, Fudan University Shanghai Cancer Center, Shanghai 200032, China; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China. ; Zhongliang Ma:1.Lab for Noncoding RNA & Cancer, School of Life Sciences, Shanghai University, Shanghai 200444, China. Electronic address: zlma@shu.edu.cn. ; Gong Yang:1.Cancer Institute, Fudan University Shanghai Cancer Center, Shanghai 200032, China; Department of Oncology, Shanghai Medical College, Fuda
期刊:MicroRNA-1251-5p Promotes Carcinogenesis and Autophagy via Targeting the Tumor Suppressor TBCC in Ovarian Cancer Cells
DOI:10.1016/j.ymthe.2019.06.005
影响因子 :12.4
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Electrical stimulation activates calpain 2 and subsequently upregulates collagens via the integrin β1/TGF-β1 signaling pathway (2003-09-20)
作者:Yang Li:1.Department of Gynecology and Obstetrics, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, PR China. ; Cheng Liu:1.Department of Gynecology and Obstetrics, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, PR China. ; Bingshu Li:1.Department of Gynecology and Obstetrics, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, PR China. ; Shasha Hong:1.Department of Gynecology and Obstetrics, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, PR China. ; Jie Min:1.Department of Gynecology and Obstetrics, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, PR China. ; Ming Hu:1.Department of Gynecology and Obstetrics, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, PR China. ; Jianming Tang:1.Department of Gynecology and Obstetrics, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, PR China. ; Tingting Wang:1.Department of Gynecology and Obstetrics, Renmin Hosp
期刊:Electrical stimulation activates calpain 2 and subsequently upregulates collagens via the integrin β1/TGF-β1 signaling pathway
DOI:10.1016/j.cellsig.2019.03.023
影响因子 :4.8
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