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小鼠肺微血管内皮细胞
Mouse Pulmonary Microvascular Endothelial Cells
货号：CP-M001
规格：5×10⁵Cells/T25培养瓶
价格： ￥3880
培养基货号： CM-M001
产品概述
小鼠肺微血管内皮细胞
Cat NO.: CP-M001
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产品名称：小鼠肺微血管内皮细胞
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组织来源：肺组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
小鼠肺微血管内皮细胞分离自肺组织；肺是机体的呼吸器官，位于胸腔，左右各一，覆盖于心之上。肺有分叶，左二右三，共五叶。肺经肺系(指气管、支气管等)与喉、鼻相连，故称喉为肺之门户，鼻为肺之外窍。微血管内皮细胞密切参与包括再生、发育、伤口愈合等一系列生理及炎症反应。细胞呈梭形或多角形，形成单层后呈鹅卵石样或铺路石样排列。肺微血管内皮细胞构成半选择性屏障，该屏障对于肺气体交换，调节液体和可溶物在血液与肺间质之间的流动具有重要意义。
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方法简介：
普诺赛实验室分离的小鼠肺微血管内皮细胞采用组织贴块法并结合内皮细胞专用培养基培养筛选制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的小鼠肺微血管内皮细胞经CD31免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 PLL（0.1mg/ml）或明胶（0.1%） 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-M001 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 内皮细胞样 传代特性 可传1-2代 消化液 0.25%胰蛋白酶 小鼠肺微血管内皮细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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Long noncoding RNA ANRIL alleviates hypoxia-induced pulmonary microvascular endothelial cell damage (2024-03-30)
作者：Yijin Qi:1.School of Medicine, Xizang Minzu University, Xianyang, Shaanxi, China. ; Mingyue Chen:1.School of Medicine, Xizang Minzu University, Xianyang, Shaanxi, China. ; Tianyi Zhang:1.School of Medicine, Xizang Minzu University, Xianyang, Shaanxi, China. ; Beibei Zhao:1.School of Medicine, Xizang Minzu University, Xianyang, Shaanxi, China. ; Tianbo Jin:1.School of Medicine, Xizang Minzu University, Xianyang, Shaanxi, China. ; Dongya Yuan:1.School of Medicine, Xizang Minzu University, Xianyang, Shaanxi, China. ;
期刊：Long noncoding RNA ANRIL alleviates hypoxia-induced pulmonary microvascular endothelial cell damage
影响因子 ：5.5
引用产品： 小鼠肺微血管内皮细胞
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Bone mesenchymal stem cell-derived exosomal miR-26a-3p promotes autophagy to attenuate LPS-induced apoptosis and inflammation in pulmonary microvascular endothelial cells (2024-02-29)
作者：Qianfei Wang:1.Department of Emergency, The First People's Hospital of Xiaoshan District, Hangzhou, Hangzhou, 311200, Zhejiang, China. wangqianfei_edu@outlook.com. ; Yiming Huang:1.Department of Emergency, The First People's Hospital of Xiaoshan District, Hangzhou, Hangzhou, 311200, Zhejiang, China. dongyiming_edu@outlook.com. ; Zherui Fu:1.Department of Emergency, The First People's Hospital of Xiaoshan District, Hangzhou, Hangzhou, 311200, Zhejiang, China. fuzherui_edu@outlook.com. ;
期刊：Bone mesenchymal stem cell-derived exosomal miR-26a-3p promotes autophagy to attenuate LPS-induced apoptosis and inflammation in pulmonary microvascular endothelial cells
影响因子 ：1.6
引用产品： 小鼠肺微血管内皮细胞 , 小鼠骨髓间充质干细胞 , 小鼠骨髓间充质干细胞完全培养基 , 小鼠骨髓间充质干细胞成骨诱导分化培养基 , 小鼠骨髓间充质干细胞成脂诱导分化培养基 , 小鼠骨髓间充质干细胞成软骨诱导分化培养基
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TFAM-Mediated mitochondrial transfer of MSCs improved the permeability barrier in sepsis-associated acute lung injury (2023-04-15)
作者：Feng Zhang:1.Department of Intensive Care Unit, the First Affiliated Hospital of Jinan University, No.613, West Huangpu Avenue, Guangzhou, 510630, Guangdong, China. ; Xinglong Zheng:1.Department of Intensive Care Unit, the First Affiliated Hospital of Jinan University, No.613, West Huangpu Avenue, Guangzhou, 510630, Guangdong, China. ; Fengzhi Zhao:1.Department of Intensive Care Unit, the First Affiliated Hospital of Jinan University, No.613, West Huangpu Avenue, Guangzhou, 510630, Guangdong, China. ; Longzhu Li:1.Department of Intensive Care Unit, the First Affiliated Hospital of Jinan University, No.613, West Huangpu Avenue, Guangzhou, 510630, Guangdong, China. ; Yinlong Ren:1.Department of Intensive Care Unit, the First Affiliated Hospital of Jinan University, No.613, West Huangpu Avenue, Guangzhou, 510630, Guangdong, China. ; Lijun Li:1.Department of Intensive Care Unit, the First Affiliated Hospital of Jinan University, No.613, West Huangpu Avenue, Guangzhou, 510630, Guangdon
期刊：TFAM-Mediated mitochondrial transfer of MSCs improved the permeability barrier in sepsis-associated acute lung injury
DOI：10.1007/s10495-023-01847-z
影响因子 ：7.2
引用产品： 小鼠骨髓间充质干细胞 , 小鼠肺微血管内皮细胞 , 小鼠肺微血管内皮细胞完全培养基
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Endothelial Microparticles Derived from Primary Pulmonary Microvascular Endothelial Cells Mediate Lung Inflammation in Chronic Obstructive Pulmonary Disease by Transferring microRNA-126 (2002-02-20)
作者：Yiming Ma:1.Department of Respiratory and Critical Care Medicine, The Second Xiangya Hospital of Central South University, Changsha, People's Republic of China. ; Xue He:1.Department of Respiratory and Critical Care Medicine, The Second Xiangya Hospital of Central South University, Changsha, People's Republic of China. ; Xiangming Liu:1.Department of Respiratory and Critical Care Medicine, The Second Xiangya Hospital of Central South University, Changsha, People's Republic of China. ; Yingjiao Long:1.Department of Respiratory and Critical Care Medicine, The Second Xiangya Hospital of Central South University, Changsha, People's Republic of China. ; Yan Chen:1.Department of Respiratory and Critical Care Medicine, The Second Xiangya Hospital of Central South University, Changsha, People's Republic of China. ;
期刊：Endothelial Microparticles Derived from Primary Pulmonary Microvascular Endothelial Cells Mediate Lung Inflammation in Chronic Obstructive Pulmonary Disease by Transferring microRNA-126
影响因子 ：4.5
引用产品： 小鼠肺微血管内皮细胞
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LncRNA Gm16410 regulates PM2.5-induced lung Endothelial-Mesenchymal Transition via the TGF-β1/Smad3/p-Smad3 pathway (2009-00-20)
作者：Kexin Ma:1.College of Medical Laboratory, Dalian Medical University, Dalian, 116044, Liaoning Province, China; Department of Clincal Laboratory, The Fourth People's Hospital of Shenyang, Shenyang, 110031, Liaoning Province, China. ; Cailong Li:1.Department of Neurosurgery, Dalian Central Hospital, Dalian, 116033, Liaoning Province, China. ; Jingbin Xu:1.College of Medical Laboratory, Dalian Medical University, Dalian, 116044, Liaoning Province, China. ; Fei Ren:1.College of Medical Laboratory, Dalian Medical University, Dalian, 116044, Liaoning Province, China. ; Xin Xu:1.College of Medical Laboratory, Dalian Medical University, Dalian, 116044, Liaoning Province, China. ; Chaosheng Liu:1.College of Medical Laboratory, Dalian Medical University, Dalian, 116044, Liaoning Province, China. ; Ben Niu:1.College of Medical Laboratory, Dalian Medical University, Dalian, 116044, Liaoning Province, China. ; Fasheng Li:1.College of Medical Laboratory, Dalian Medical University, Dalian, 116
期刊：LncRNA Gm16410 regulates PM2.5-induced lung Endothelial-Mesenchymal Transition via the TGF-β1/Smad3/p-Smad3 pathway
DOI：10.1016/j.ecoenv.2020.111327
影响因子 ：6.8
引用产品： 小鼠肺微血管内皮细胞
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Endothelial progenitor cell transplantation attenuates lipopolysaccharide-induced acute lung injury via regulating miR-10a/b-5p (2006-09-20)
作者：Yan Jin:1.Department of Emergency, The First Affiliated Hospital of China Medical University, No.155 Nanjing North Street, Heping District, Shenyang, Liaoning Province, 110001, People's Republic of China. ; Chen Yang:1.Department of Emergency, The First Affiliated Hospital of China Medical University, No.155 Nanjing North Street, Heping District, Shenyang, Liaoning Province, 110001, People's Republic of China. ; Xintong Sui:1.Department of Emergency, The First Affiliated Hospital of China Medical University, No.155 Nanjing North Street, Heping District, Shenyang, Liaoning Province, 110001, People's Republic of China. ; Quan Cai:1.Department of Emergency, The First Affiliated Hospital of China Medical University, No.155 Nanjing North Street, Heping District, Shenyang, Liaoning Province, 110001, People's Republic of China. ; Liang Guo:1.Department of Emergency, The First Affiliated Hospital of China Medical University, No.155 Nanjing North Street, Heping District, Shenyang, Liaoning
期刊：Endothelial progenitor cell transplantation attenuates lipopolysaccharide-induced acute lung injury via regulating miR-10a/b-5p
影响因子 ：4.5
引用产品： 小鼠肺微血管内皮细胞
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