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小鼠骨髓间充质干细胞成软骨诱导分化培养基专门为小鼠骨髓间充质干细胞成软骨诱导分化而开发，针对小鼠骨髓间充质干细胞的特性优化分化试剂的 配方，可增加小鼠骨髓间充质干细胞的成软骨分化效果。
本产品含血清成分，仅用于科研用途，不 可用于诊断、治疗、临床及其他用途。
成软骨诱导分化培养基：
成分名称 | 100 mL 添加体积 | 200 mL 添加体积 |
成软骨分化专用基础培养基 Basal Medium For Stem Cells Chondrogenic Differentiation | 87.6 mL | 175.2 mL |
成软骨分化专用胎牛血清（FBS） Fetal Bovine Serum For Stem Cells Chondrogenic Differentiation | 10 mL | 20 mL |
小鼠骨髓间充质干细胞成软骨分化添加物 Supplement For mBMSCs Chondrogenic Differentiation | 2.4 mL | 2.4 mL×2 |
辅助试剂：
成分名称 | 100 mL 添加体积 | 200 mL 添加体积 |
阿尔新兰染色液 Alcian Blue 8GX Solution | 10 mL | 10 mL×2 |
明胶包被液 Gelatin Solution | 10 mL | 10mL×2 |
注：各成分请根据试剂管上标签标示温度保存。
产品说明
| 产品规格 | 100mL/200mL |
| 储存条件 | 按标签所示温度保存，避光储存 |
| 运输条件 | 冰袋冷藏运输 |
| 有效期 | 一年 |
注意事项
1. 因为培养基的成分较多，请在配制过程中严格注意无菌操作。
2. 成软骨分化添加物中含细胞生长因子，务必现配现用，配好的完全培养基必须当天用完。
3.血清离心：血清中可能会存在白色絮状沉淀物，这通常是胆固醇、脂肪酸酯以及一些蛋白质析出导致，属于正常现象，该现象不影响细胞的培养和诱导分化。若您欲去除这些絮状沉淀物，可以将血清分装至无菌离心管内，以400-600 g离心5 min，取上清液使用。但是我们不建议您以过滤的方法去除这些絮状沉淀物，一方面它可能会阻塞您的过滤膜；另一方面，过滤血清这种行为可能会导致血清中部分营养成分的流失。
参考文献
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Ptip safeguards the epigenetic control of skeletal stem cell quiescence and potency in skeletogenesis (2024-02-29)
作者：Jianfei Liang:1.State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan 430079, China; Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an 710004, China; Department of Implant Dentistry, College of Stomatology, Xi'an Jiaotong University, Xi'an 710004, China; State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Key Laboratory of Stomatology, Shaanxi International Joint Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, China. ; Jing Wang:1.State Key Laboratory of Oral & Maxillofacial Reconstruction and Regener
期刊：Ptip safeguards the epigenetic control of skeletal stem cell quiescence and potency in skeletogenesis
DOI：10.1016/j.scib.2024.02.036
影响因子 ：18.9
引用产品： 小鼠骨髓间充质干细胞成软骨诱导分化培养基
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Mesenchymal stem cells overexpressing XIST induce macrophage M2 polarization and improve neural stem cell homeostatic microenvironment, alleviating spinal cord injury (2024-01-10)
作者：Dan Zhu:1.Hubei Key Laboratory of Diabetes and Angiopathy, Xianning Medical College, Hubei University of Science and Technology, Xianning, P.R. China. ; Tie Peng:1.Xianning Medical College, Hubei University of Science and Technology, Xianning, P.R. China. ; Zhenwang Zhang:1.Hubei Key Laboratory of Diabetes and Angiopathy, Xianning Medical College, Hubei University of Science and Technology, Xianning, P.R. China. ; Shuang Guo:1.Hubei Key Laboratory of Diabetes and Angiopathy, Xianning Medical College, Hubei University of Science and Technology, Xianning, P.R. China. ; Ying Su:1.Xianning Medical College, Hubei University of Science and Technology, Xianning, P.R. China. ; Kangwei Zhang:1.Xianning Medical College, Hubei University of Science and Technology, Xianning, P.R. China. ; Jiawei Wang:1.Hubei Key Laboratory of Diabetes and Angiopathy, Xianning Medical College, Hubei University of Science and Technology, Xianning, P.R. China. ; Chao Liu:1.Hubei Key Laboratory of Diabetes and
期刊：Mesenchymal stem cells overexpressing XIST induce macrophage M2 polarization and improve neural stem cell homeostatic microenvironment, alleviating spinal cord injury
影响因子 ：8.2
引用产品： 小鼠骨髓来源巨噬细胞 , 小鼠骨髓间充质干细胞 , 小鼠骨髓间充质干细胞成骨诱导分化培养基 , 小鼠骨髓间充质干细胞成脂诱导分化培养基 , 小鼠骨髓间充质干细胞成软骨诱导分化培养基
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Apical papilla stem cell-derived exosomes regulate lipid metabolism and alleviate inflammation in the MCD-induced mouse NASH model (2024-02-21)
作者：Yi-Fei Nie:1.Department of Prosthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Research Center of Dental Materials and Oral Tissue Regeneration & Shandong Provincial Clinical Research Center for Oral Diseases, Jinan, Shandong, China. ; Jia-Ming Shang:1.Department of Prosthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Research Center of Dental Materials and Oral Tissue Regeneration & Shandong Provincial Clinical Research Center for Oral Diseases, Jinan, Shandong, China. ; Duan-Qin Liu:1.Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Research Center of Dental Materials and Oral Ti
期刊：Apical papilla stem cell-derived exosomes regulate lipid metabolism and alleviate inflammation in the MCD-induced mouse NASH model
影响因子 ：5.8
引用产品： 小鼠骨髓间充质干细胞成软骨诱导分化培养基
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Bone mesenchymal stem cell-derived exosomal miR-26a-3p promotes autophagy to attenuate LPS-induced apoptosis and inflammation in pulmonary microvascular endothelial cells (2024-02-29)
作者：Qianfei Wang:1.Department of Emergency, The First People's Hospital of Xiaoshan District, Hangzhou, Hangzhou, 311200, Zhejiang, China. wangqianfei_edu@outlook.com. ; Yiming Huang:1.Department of Emergency, The First People's Hospital of Xiaoshan District, Hangzhou, Hangzhou, 311200, Zhejiang, China. dongyiming_edu@outlook.com. ; Zherui Fu:1.Department of Emergency, The First People's Hospital of Xiaoshan District, Hangzhou, Hangzhou, 311200, Zhejiang, China. fuzherui_edu@outlook.com. ;
期刊：Bone mesenchymal stem cell-derived exosomal miR-26a-3p promotes autophagy to attenuate LPS-induced apoptosis and inflammation in pulmonary microvascular endothelial cells
影响因子 ：1.6
引用产品： 小鼠肺微血管内皮细胞 , 小鼠骨髓间充质干细胞 , 小鼠骨髓间充质干细胞完全培养基 , 小鼠骨髓间充质干细胞成骨诱导分化培养基 , 小鼠骨髓间充质干细胞成脂诱导分化培养基 , 小鼠骨髓间充质干细胞成软骨诱导分化培养基
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