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小鼠骨髓间充质干细胞成脂诱导分化培养基专门为小鼠骨髓间充质干细胞成脂诱导分化而开发，针对小鼠骨髓间充质干细胞的特性优化分化试剂的配方，可增加小鼠骨髓间充质干细胞的成脂分化效果。
本产品含血清成分，仅用于科研用途，不可用于诊断、治疗、临床及其他用途。
成脂诱导分化培养基A液：
成分名称 | 添加体积 |
成脂分化专用基础培养基A Basal Medium For Stem Cells Adipogenic Differentiation A | 177 mL |
成脂分化专用胎牛血清（FBS） Fetal Bovine Serum For Stem Cells Adipogenic Differentiation | 20 mL |
小鼠骨髓间充质干细胞成脂分化添加物A① Supplement For mBMSCs Adipogenic Differentiation A① | 2.8 mL |
小鼠骨髓间充质干细胞成脂分化添加物A② Supplement For mBMSCs Adipogenic Differentiation A② | 200 μL |
成脂诱导分化培养基B液：
成分名称 | 添加体积 |
成脂分化专用基础培养基B Basal Medium For Stem Cells Adipogenic Differentiation B | 177.2 mL |
成脂分化专用胎牛血清（FBS） Fetal Bovine Serum For Stem Cells Adipogenic Differentiation | 20 mL |
小鼠骨髓间充质干细胞成脂分化添加物B Supplement For mBMSCs Adipogenic Differentiation B | 2.8 mL |
辅助试剂：
成分名称 | 添加体积 |
油红O染色液 Oil Red O Solution | 10 mL |
明胶包被液 Gelatin Solution | 10 mL |
注：各成分请根据试剂管上标签标示温度保存。
产品说明
| 产品规格 | 400mL |
| 储存条件 | 按标签所示温度保存，避光储存 |
| 运输条件 | 冰袋冷藏运输 |
| 有效期 | 一年 |
注意事项
1. 因为培养基的成分较多，请在配制过程中严格注意无菌操作。
2. A、B交替诱导是为了减轻A液中试剂对干细胞的影响，如果您的干细胞状态较好可在前7天先只使用A液进行刺激诱导（中途每2~3天更换新鲜A液），待脂滴快速出现后，再进行两种培养基交替诱导的操作。
3.血清离心：血清中可能会存在白色絮状沉淀物，这通常是胆固醇、脂肪酸酯以及一些蛋白质析出导致，属于正常现象，该现象不影响细胞的培养和诱导分化。若您欲去除这些絮状沉淀物，可以将血清分装至无菌离心管内，以400-600 g离心5 min，取上清液使用。但是我们不建议您以过滤的方法去除这些絮状沉淀物，一方面它可能会阻塞您的过滤膜；另一方面，过滤血清这种行为可能会导致血清中部分营养成分的流失。
参考文献
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Mesenchymal stem cells overexpressing XIST induce macrophage M2 polarization and improve neural stem cell homeostatic microenvironment, alleviating spinal cord injury (2024-01-10)
作者：Dan Zhu:1.Hubei Key Laboratory of Diabetes and Angiopathy, Xianning Medical College, Hubei University of Science and Technology, Xianning, P.R. China. ; Tie Peng:1.Xianning Medical College, Hubei University of Science and Technology, Xianning, P.R. China. ; Zhenwang Zhang:1.Hubei Key Laboratory of Diabetes and Angiopathy, Xianning Medical College, Hubei University of Science and Technology, Xianning, P.R. China. ; Shuang Guo:1.Hubei Key Laboratory of Diabetes and Angiopathy, Xianning Medical College, Hubei University of Science and Technology, Xianning, P.R. China. ; Ying Su:1.Xianning Medical College, Hubei University of Science and Technology, Xianning, P.R. China. ; Kangwei Zhang:1.Xianning Medical College, Hubei University of Science and Technology, Xianning, P.R. China. ; Jiawei Wang:1.Hubei Key Laboratory of Diabetes and Angiopathy, Xianning Medical College, Hubei University of Science and Technology, Xianning, P.R. China. ; Chao Liu:1.Hubei Key Laboratory of Diabetes and
期刊：Mesenchymal stem cells overexpressing XIST induce macrophage M2 polarization and improve neural stem cell homeostatic microenvironment, alleviating spinal cord injury
影响因子 ：8.2
引用产品： 小鼠骨髓来源巨噬细胞 , 小鼠骨髓间充质干细胞 , 小鼠骨髓间充质干细胞成骨诱导分化培养基 , 小鼠骨髓间充质干细胞成脂诱导分化培养基 , 小鼠骨髓间充质干细胞成软骨诱导分化培养基
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Bone mesenchymal stem cell-derived exosomal miR-26a-3p promotes autophagy to attenuate LPS-induced apoptosis and inflammation in pulmonary microvascular endothelial cells (2024-02-29)
作者：Qianfei Wang:1.Department of Emergency, The First People's Hospital of Xiaoshan District, Hangzhou, Hangzhou, 311200, Zhejiang, China. wangqianfei_edu@outlook.com. ; Yiming Huang:1.Department of Emergency, The First People's Hospital of Xiaoshan District, Hangzhou, Hangzhou, 311200, Zhejiang, China. dongyiming_edu@outlook.com. ; Zherui Fu:1.Department of Emergency, The First People's Hospital of Xiaoshan District, Hangzhou, Hangzhou, 311200, Zhejiang, China. fuzherui_edu@outlook.com. ;
期刊：Bone mesenchymal stem cell-derived exosomal miR-26a-3p promotes autophagy to attenuate LPS-induced apoptosis and inflammation in pulmonary microvascular endothelial cells
影响因子 ：1.6
引用产品： 小鼠肺微血管内皮细胞 , 小鼠骨髓间充质干细胞 , 小鼠骨髓间充质干细胞完全培养基 , 小鼠骨髓间充质干细胞成骨诱导分化培养基 , 小鼠骨髓间充质干细胞成脂诱导分化培养基 , 小鼠骨髓间充质干细胞成软骨诱导分化培养基
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GKT137831 in combination with adipose-derived stem cells alleviates high glucose-induced inflamm-aging and improves diabetic wound healing (2023-09-29)
作者：Yunxian Dong:1.Department of Plastic and Reconstructive Surgery, Guangdong Second Provincial General Hospital, No. 466 Middle Xingang Road, Guangzhou, Guangdong Province 510317, China. ; 2.Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern Medical University, No. 1838 Guangzhou North Road, Guangzhou, Guangdong Province 510515, China. ; Youliang Zhang:1.Department of Plastic and Reconstructive Surgery, Guangdong Second Provincial General Hospital, No. 466 Middle Xingang Road, Guangzhou, Guangdong Province 510317, China. ; Fangwei Li:1.Department of Plastic and Reconstructive Surgery, Guangdong Second Provincial General Hospital, No. 466 Middle Xingang Road, Guangzhou, Guangdong Province 510317, China. ; Bing Tang:1.Department of Burn Surgery, First Affiliated Hospital of Sun Yat-sen University, No. 58 Zhongshan Er Road, Guangzhou, Guangdong Province 510080, China. ; Dongming Lv:1.Department of Burn Surgery, First Affiliated Hospital of Sun Yat-sen University, No.
期刊：GKT137831 in combination with adipose-derived stem cells alleviates high glucose-induced inflamm-aging and improves diabetic wound healing
影响因子 ：5.5
引用产品： 小鼠骨髓间充质干细胞成脂诱导分化培养基
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Human umbilical cord mesenchymal stem cells derived extracellular vesicles alleviate salpingitis by promoting M1–to–M2 transformation (2023-02-16)
作者：Changlin Zhang:1.Pelvic Floor Disorders Center, Scientific Research Center, Department of Gynecology, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, China. ; Wei Liao:1.Pelvic Floor Disorders Center, Scientific Research Center, Department of Gynecology, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, China. ; Weizhao Li:1.Pelvic Floor Disorders Center, Scientific Research Center, Department of Gynecology, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, China. ; Mengxiong Li:1.Pelvic Floor Disorders Center, Scientific Research Center, Department of Gynecology, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, China. ; Xiaoyu Xu:1.Pelvic Floor Disorders Center, Scientific Research Center, Department of Gynecology, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, China. ; Haohui Sun:1.Sun Yat-Sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborativ
期刊：Human umbilical cord mesenchymal stem cells derived extracellular vesicles alleviate salpingitis by promoting M1–to–M2 transformation
DOI：10.3389/fphys.2023.1131701
影响因子 ：4.0
引用产品： 特级胎牛血清 , 小鼠骨髓间充质干细胞成骨诱导分化培养基 , 小鼠骨髓间充质干细胞成脂诱导分化培养基
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