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产品概述
大鼠软骨细胞
Cat NO.: CP-R087
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产品名称：大鼠软骨细胞
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组织来源：软骨组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
大鼠软骨细胞分离自软骨组织；软骨组织由软骨细胞、基质及纤维构成。软骨组织再生能力强，这些增生的幼稚细胞形似纤维母细胞，以后逐渐变为软骨母细胞，并形成软骨基质，细胞被埋在软骨陷窝内而变为静止的软骨细胞。根据软骨组织内所含纤维成分的不同，可将软骨分为透明软骨、弹性软骨和纤维软骨三种，其中以透明软骨的分布较广，结构也较典型。软骨细胞(chondrocyte)位于软骨陷窝内。幼稚的软骨细胞位于软骨组织的表层，单个分布，体积较小，呈椭圆形，长轴与软骨表面平行，越向深层的软骨细胞体积之间增大呈圆形，细胞核圆形或卵圆形，染色浅，细胞质弱嗜碱性，常见数量不一的脂滴。成熟的软骨细胞多2-8个成群分布于软骨陷窝内，这些软骨细胞由同一个母细胞分裂增殖而成，称为同源细胞群。电镜下，软骨细胞有突起和皱褶，细胞质内有大量的粗面内质网和发达的高尔基复合体及少量的线粒体。在组织切片中，软骨细胞收缩为不规则形，在软骨囊和细胞之间出现较大的腔隙。体外培养的软骨细胞对于研究其生理功能、药物作用以及各种致病因素作用下的病理生理改变具重要意义。
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方法简介：
普诺赛实验室分离的大鼠软骨细胞采用胶原酶 - 中性蛋白酶联合消化制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的大鼠软骨细胞经Ⅱ型胶原蛋白免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-R087 换液频率 每3-4天换液一次 生长特性 贴壁 细胞形态 梭形、多角形 传代特性 可传5代左右；3代以内状态最佳 消化液 0.25%胰蛋白酶 大鼠软骨细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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Albiflorin alleviation efficacy in osteoarthritis injury using in-vivo and in-vitro models (2023-07-04)
作者：Feng Zhou:1.Department of Orthopedics & Soft Tissue, Hunan Cancer Hospital, Changsha, Hunan, P.R. China. ; Jianfan Liu:1.Department of Orthopedics & Soft Tissue, Hunan Cancer Hospital, Changsha, Hunan, P.R. China. ; Xuezheng Xu:1.Department of Orthopedics & Soft Tissue, Hunan Cancer Hospital, Changsha, Hunan, P.R. China. ; Yi Luo:1.Department of Orthopedics & Soft Tissue, Hunan Cancer Hospital, Changsha, Hunan, P.R. China. ; Shuo Yang:1.Department of Orthopedics & Soft Tissue, Hunan Cancer Hospital, Changsha, Hunan, P.R. China. ;
期刊：Albiflorin alleviation efficacy in osteoarthritis injury using in-vivo and in-vitro models
影响因子 ：3.3
引用产品： 大鼠软骨细胞
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Baicalin mitigated IL-1β-Induced osteoarthritis chondrocytes damage through activating mitophagy (2023-02-08)
作者：Jun He:1.Second Department of Orthopedics, Zhejiang Hospital, Hangzhou, China. ; Jian He:1.Second Department of Orthopedics, Zhejiang Hospital, Hangzhou, China. ;
期刊：Baicalin mitigated IL-1β-Induced osteoarthritis chondrocytes damage through activating mitophagy
影响因子 ：3.0
引用产品： 大鼠软骨细胞 , 大鼠软骨细胞完全培养基
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Rapamycin incorporating hydrogel improves the progression of osteoarthritis by inducing synovial macrophages polarization and reducing intra-articular inflammation (2022-12-24)
作者：Bo Chen: 1.Rehabilitation Medicine Department, Northern Jiangsu People’s Hospital, Yangzhou, Jiangsu 225001, China; Jibing Wang: 1.Rehabilitation Medicine Department, Northern Jiangsu People’s Hospital, Yangzhou, Jiangsu 225001, China; Xing Jin: 1.Rehabilitation Medicine Department, Northern Jiangsu People’s Hospital, Yangzhou, Jiangsu 225001, China; Xin Wang: 1.Rehabilitation Medicine Department, Northern Jiangsu People’s Hospital, Yangzhou, Jiangsu 225001, China; Zhangling Fan: 1.Rehabilitation Medicine Department, Northern Jiangsu People’s Hospital, Yangzhou, Jiangsu 225001, China; Yuxin Zhang: 1.Department of Oral Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China; Xuehan Sang: 1.Rehabilitation Medicine Department, Northern Jiangsu People’s
期刊：Rapamycin incorporating hydrogel improves the progression of osteoarthritis by inducing synovial macrophages polarization and reducing intra-articular inflammation
DOI：10.1016/j.matdes.2022.111542
影响因子 ：9.4
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Cationic solid lipid nanoparticles loaded by integrin β1 plasmid DNA attenuates IL-1β-induced apoptosis of chondrocyte (2011-00-20)
作者：Yuejiang Zhao:1.The First Department of Orthopedics, Cangzhou Central Hospital, Cangzhou, Hebei Province, China. ; Hanwen Chen:1.The First Department of Orthopedics, Cangzhou Central Hospital, Cangzhou, Hebei Province, China. ; Lu Wang:1.The First Department of Orthopedics, Cangzhou Central Hospital, Cangzhou, Hebei Province, China. ; Zhiyuan Guo:1.The First Department of Orthopedics, Cangzhou Central Hospital, Cangzhou, Hebei Province, China. ; Shijie Liu:1.Department of Joint Surgery, Xingtan Hospital Affiliated to Shunde Hospital of Southern Medical University, Foshan, Guangdong Province, China. ; Simin Luo:1.Department of Bone and Joint Surgery, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong Province, China. ;
期刊：Cationic solid lipid nanoparticles loaded by integrin β1 plasmid DNA attenuates IL-1β-induced apoptosis of chondrocyte
影响因子 ：5.2
引用产品： 大鼠软骨细胞
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