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  • 人肾小球内皮细胞
  • 人肾小球内皮细胞

人肾小球内皮细胞
Human Glomerular Endothelial Cells

货号:CP-H061

规格:5×10⁵Cells/T25培养瓶

价格: ¥7380

培养基货号: CM-H061

数量: - +
  • 人肾小球内皮细胞
  • 人肾小球内皮细胞
产品概述

人肾小球内皮细胞

Cat NO.: CP-H061

  1. 产品名称:人肾小球内皮细胞

  2. 组织来源:肾组织

  3. 产品规格:5×10⁵Cells/T25培养瓶

  4. 细胞简介:

    人肾小球内皮细胞分离自肾组织;肾脏是机体的重要器官,它的基本功能是生成尿液,借以清除体内代谢产物及某些废物、毒物,同时经重吸收功能保留水份及其他有用物质,如葡萄糖、蛋白质、氨基酸、钠离子、钾离子、碳酸氢钠等,以调节水、电解质平衡及维护酸碱平衡。肾脏同时还有内分泌功能,生成肾素、促红细胞生成素、活性维生素D3、前列腺素、激肽等,又为机体部分内分泌激素的降解场所和肾外激素的靶器官。肾脏的这些功能,保证了机体内环境的稳定,使新陈代谢得以正常进行。肾小球是肾元中的用于将血液过滤生成原尿的一团毛细血丛,被鲍氏囊所包裹,是尿液形成的重要构造。血液经由入球小动脉进入肾小球。肾小球内的微血管不像其他微血管,汇流入静脉,而是流入出球小动脉。在肾小球内,微血管受到高压,而加速了超滤作用(hyperfiltration)的进行。微血管中的血液经由超滤作用之后,形成滤液,渗入鲍氏囊内。肾小球与鲍氏囊合称为肾小体,肾小球的过滤速率便称为肾小球过滤率。肾小球内皮细胞是一类特殊微血管类型的细胞。细胞为圆形、多角形,单层贴壁生长;细胞质丰富,细胞核为圆形或卵圆形。肾小球内皮细胞被覆于肾小球毛细血管壁腔侧,与血流直接接触,是肾小球滤过膜的第一道屏障,可粘附细菌和白细胞,修复基底膜,并有抗凝、抗血栓的作用;所以,体外培养的肾小球内皮细胞在免疫学和病理生理学领域中具有重要的研究价值。

  5. 方法简介:

    普诺赛实验室分离的人肾小球内皮细胞采用先机械研磨过不锈钢网筛分离得到肾小球、后用胶原酶消化,结合内皮细胞专用培养基培养筛选制备而来,细胞总量约为5×10⁵cells/瓶。

  6. 质量检测:

    普诺赛实验室分离的人肾小球内皮细胞经CD31免疫荧光鉴定,纯度可达90%以上,且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。

  7. 培养信息:

    包被条件PLL(0.1mg/ml)或明胶(0.1%)
    培养基含FBS、生长添加剂、Penicillin、Streptomycin等
    产品货号CM-H061
    换液频率每2-3天换液一次
    生长特性贴壁
    细胞形态内皮细胞样
    传代特性可传2-3代
    消化液0.25%胰蛋白酶

    人肾小球内皮细胞体外培养周期有限;建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养,以此保证该细胞的最佳培养状态。

参考文献
文献列表
  • Clinical Significance of Apela in Acute Cardiorenal Insuffiency of Chronic Heart Failure (2024-01-18)

    作者:Yani Zong:1.Department of Cardiovascular Medicine, The Affiliated Brain Hospital of Nanjing Medical University, Nanjing, China. ; Yajie Wang:1.Department of Neurology, The Affiliated Brain Hospital of Nanjing Medical University, Nanjing, China. ; Yuexin Hu:1.Department of Cardiovascular Medicine, The Affiliated Brain Hospital of Nanjing Medical University, Nanjing, China. ; Zhi Wang:1.Department of Cardiovascular Medicine, The Affiliated Brain Hospital of Nanjing Medical University, Nanjing, China. ;

    期刊:Clinical Significance of Apela in Acute Cardiorenal Insuffiency of Chronic Heart Failure

    DOI:10.1159/000536316

    影响因子 :2.8

    引用产品: THP-1 细胞 ,  人肾小球内皮细胞 ,  人肾小球系膜细胞 ,  HK-2 细胞 ,  DMEM高糖 培养基 ,  人肾小球内皮细胞完全培养基 ,  人肾小球系膜细胞完全培养基 ,  人肾小管上皮细胞完全培养基

  • STAT5A modulated EndMT via upregulation of ELTD1 expression in diabetic nephropathy (2004-02-20)

    作者:Ye Tian:1.Department of Anesthesiology, The Sixth Medical Center of Chinese PLA General Hospital, Beijing, China. ; Zhenhua Bi:1.Department of Anesthesiology, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China. ; Shuaina Ge:1.Department of Anesthesiology, The Sixth Medical Center of Chinese PLA General Hospital, Beijing, China. ; Bo Ye:1.Department of Anesthesiology, Air Force Medical Center, Beijing, China. ; Wenjie Han:1.Department of Geriatric Medicine, The Sixth Medical Center of Chinese PLA General Hospital, Beijing, China. ;

    期刊:STAT5A modulated EndMT via upregulation of ELTD1 expression in diabetic nephropathy

    DOI:10.1111/1440-1681.13644

    影响因子 :2.9

    引用产品: 人肾小球内皮细胞

  • Silencing circ_0080425 alleviates high-glucose-induced endothelial cell dysfunction in diabetic nephropathy by targeting miR-140-3p/FN1 axis (2022-09-09)

    作者:Linping Zhang:1.Kidney Disease and Dialysis Center, Shaanxi Provincial People's Hospital, No. 256 Youyi West Road, Beilin District, Xi'an, 710068, Shaanxi, China. ; Gang Jin:1.Kidney Disease and Dialysis Center, Shaanxi Provincial People's Hospital, No. 256 Youyi West Road, Beilin District, Xi'an, 710068, Shaanxi, China. ; Wei Zhang:1.Kidney Disease and Dialysis Center, Shaanxi Provincial People's Hospital, No. 256 Youyi West Road, Beilin District, Xi'an, 710068, Shaanxi, China. ; Xiaoming Wang:1.Kidney Disease and Dialysis Center, Shaanxi Provincial People's Hospital, No. 256 Youyi West Road, Beilin District, Xi'an, 710068, Shaanxi, China. ; Zhenjiang Li:1.Kidney Disease and Dialysis Center, Shaanxi Provincial People's Hospital, No. 256 Youyi West Road, Beilin District, Xi'an, 710068, Shaanxi, China. ; Qianlan Dong:1.Kidney Disease and Dialysis Center, Shaanxi Provincial People's Hospital, No. 256 Youyi West Road, Beilin District, Xi'an, 710068, Shaanxi, China. dcak8nf@163.com. ;

    期刊:Silencing circ_0080425 alleviates high-glucose-induced endothelial cell dysfunction in diabetic nephropathy by targeting miR-140-3p/FN1 axis

    DOI:10.1007/s10157-022-02273-2

    影响因子 :2.6

    引用产品: 人肾小球内皮细胞 ,  人肾小球内皮细胞完全培养基

  • Telmisartan Mitigates High-Glucose-Induced Injury in Renal Glomerular Endothelial Cells (rGECs) and Albuminuria in Diabetes Mice (2008-01-20)

    作者:Xiaolin Zhan:1.Department of Nephrology, Huangshi Central Hospital, Affiliated Hospital of Hubei Polytechnic University, Edong Healthcare Group, Huangshi, Hubei 435000, China. ; Wei Chen:1.Hubei Key Laboratory of Kidney Disease Pathogenesis and Intervention, Wuhan, Hubei 430000, China. ; Jian Chen:1.Department of Nephrology, Huangshi Central Hospital, Affiliated Hospital of Hubei Polytechnic University, Edong Healthcare Group, Huangshi, Hubei 435000, China. ; Changjiang Lei:1.Department of General Surgery, The Fifth Hospital of Wuhan, Wuhan, Hubei 430050, China. ; Liqin Wei:1.Department of Nephrology, Huangshi Central Hospital, Affiliated Hospital of Hubei Polytechnic University, Edong Healthcare Group, Huangshi, Hubei 435000, China. ;

    期刊:Telmisartan Mitigates High-Glucose-Induced Injury in Renal Glomerular Endothelial Cells (rGECs) and Albuminuria in Diabetes Mice

    DOI:10.1021/acs.chemrestox.1c00159

    影响因子 :4.0

    引用产品: 人肾小球内皮细胞

  • XCL1 Aggravates Diabetic Nephropathy-Mediated Renal Glomerular Endothelial Cell Apoptosis and Inflammatory Response via Regulating p53/Nuclear Factor-Kappa B Pathway (2009-01-20)

    作者:Yuan Zhang:1.Department of Nephrology, Affiliated Hospital of Nantong University, Nantong, China. ; Xiaolan Chen:1.Department of Nephrology, Affiliated Hospital of Nantong University, Nantong, China. ; Yaping Fan:1.Department of Nephrology, Affiliated Hospital of Nantong University, Nantong, China. ; Jing Liu:1.Department of Nephrology, Affiliated Hospital of Nantong University, Nantong, China. ; Li Yuan:1.Department of Nephrology, Affiliated Hospital of Nantong University, Nantong, China. ;

    期刊:XCL1 Aggravates Diabetic Nephropathy-Mediated Renal Glomerular Endothelial Cell Apoptosis and Inflammatory Response via Regulating p53/Nuclear Factor-Kappa B Pathway

    DOI:10.1159/000518172

    影响因子 :3.5

    引用产品: 人肾小球内皮细胞

  • MicroRNA-183 exerts a protective role in lupus nephritis through blunting the activation of TGF-β/Smad/TLR3 pathway via reducing Tgfbr1 (2009-00-20)

    作者:Huimeng Qi:1.Department of General Practice, The First Hospital of China Medical University, Shenyang, 110001, Liaoning, PR China. ; Qin Cao:1.Department of Gastroenterology, The First Hospital of China Medical University, Shenyang, 110001, Liaoning, PR China. ; Qiang Liu:1.Department of Nephrology, The First Hospital of China Medical University, Shenyang, 110001, Liaoning, PR China. Electronic address: torroseliu@163.com. ;

    期刊:MicroRNA-183 exerts a protective role in lupus nephritis through blunting the activation of TGF-β/Smad/TLR3 pathway via reducing Tgfbr1

    DOI:10.1016/j.yexcr.2020.112138

    影响因子 :3.7

    引用产品: 人肾小球内皮细胞

  • Astragaloside IV protects against diabetic nephropathy via activating eNOS in streptozotocin diabetes-induced rats (2012-09-20)

    作者:Yuyan Fan:1.Department of Traditional Chinese Medicine, Beijing Tiantan Hospital, Capital Medical University, 119 West Nansihuan Road, Beijing, 100070, People's Republic of China. yuyan1970@yeah.net. ; Hongyu Fan:1.Remote Consultation Center, Liaoyang Central Hospital, Liaoyang, Liaoning, 111000, People's Republic of China. ; Bin Zhu:1.Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University, Beijing, 100070, People's Republic of China. ; Yilun Zhou:1.Department of Nephrology, Beijing Tiantan Hospital, Capital Medical University, Beijing, 100070, People's Republic of China. ; Qingshan Liu:1.Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing, 100081, People's Republic of China. ; Ping Li:1.Department of Pharmacy and Pharmacology, Institute of Clinical Medicine, China-Japan Friendship Hospital, Beijing, 100029, People's Republic of China. ;

    期刊:Astragaloside IV protects against diabetic nephropathy via activating eNOS in streptozotocin diabetes-induced rats

    DOI:10.1186/s12906-019-2728-9

    影响因子 :0.0

    引用产品: 人肾小球内皮细胞完全培养基 ,  人肾小球内皮细胞

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