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人心脏微血管内皮细胞
Human Cardiac Microvascular Endothelial Cells
货号：CP-H079
规格：5×10⁵Cells/T25培养瓶
价格： ￥8880
培养基货号： CM-H079
产品概述
人心脏微血管内皮细胞
Cat NO.: CP-H079
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产品名称：人心脏微血管内皮细胞
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英文简称：CMEC,CMECs
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组织来源：心脏组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
人心脏微血管内皮细胞分离自心脏组织；心脏是脊椎动物身体中最重要的一个器官，主要功能是为血液流动提供压力，把血液运行至身体各个部分。心脏由心肌构成，左心房、左心室、右心房、右心室四个腔组成。左右心房之间和左右心室之间均由间隔隔开，故互不相通，心房与心室之间有瓣膜(房室瓣)，这些瓣膜使血液只能由心房流入心室，而不能倒流。心脏的作用是推动血液流动，向器官、组织提供充足的血流量，以供应氧和各种营养物质，并带走代谢的终产物(如二氧化碳、无机盐、尿素和尿酸等)，使细胞维持正常的代谢和功能。心脏微血管内皮细胞是组成心脏微血管腔面单层扁平上皮样细胞，它所产生和分泌的生物活性物质对维持血管张力、调节血压、抗血栓形成等有重要作用，在心脏血管疾病的发病机制中有重要病理生理学意义。近年来大量研究表明，心肌微血管内皮细胞的功能和病理改变直接影响心肌细胞功能，也是诸多毒素、炎症因子及病毒等重要靶位，其作为体外研究的细胞模型在心肌缺血-再灌注发病机制和细胞间旁分泌细胞生长因子研究中起着重要作用。
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方法简介：
普诺赛实验室分离的人心脏微血管内皮细胞采用胶原酶-中性蛋白酶混合消化法结合密度梯度离心法、最后通过内皮细胞专用培养基培养筛选制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的人心脏微血管内皮细胞经CD31免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 PLL（0.1mg/ml）或明胶（0.1%） 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-H079 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 内皮细胞样 传代特性 可传2-3代 消化液 0.25%胰蛋白酶 人心脏微血管内皮细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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Irisin links Claudin-5 preservation and Mfn2-mediated mitochondrial dynamics to resist doxorubicin-induced cardiac endothelial damage (2024-01-09)
作者：Jun He:1.Department of Cardiology, Neijiang Second People's Hospital, Neijiang, 641000, China. ; Lin Hou:1.Department of Nursing, Neijiang Second People's Hospital, Neijiang, 641000, China. ; Quanwei Liu:1.Department of Cardiology, Neijiang Second People's Hospital, Neijiang, 641000, China. ; Rui Zhou:1.National Regional Children's Medical Center (Northwest); Key Laboratory of Precision Medicine to Pediatric Diseases of Shaanxi Province; Xi'an Key Laboratory of Children's Health and Diseases, Shaanxi Institute for Pediatric Diseases; Xi'an Children's Hospital. Xi'an 710003, China. Electronic address: zhouhuaxizhu@126.com. ;
期刊：Irisin links Claudin-5 preservation and Mfn2-mediated mitochondrial dynamics to resist doxorubicin-induced cardiac endothelial damage
DOI：10.1016/j.bbrc.2024.149501
影响因子 ：3.1
引用产品： 人心脏微血管内皮细胞
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circABCA3 knockdown relieves hypoxia-induced human cardiac microvascular endothelial cell dysfunction by targeting the miR-671-5p/PCSK9 axis (2023-09-07)
作者：Hui Hui:1.Dalian Medical University, No. 9 West Section Lvshun South Road, Dalian, 116044, China. ; 2.Department of Cardiology, Dalian Municipal Central Hospital, Dalian, China. ; Gaowa Zhao:1.Department of Cardiology, Affiliated Zhongshan Hospital of Dalian University, No. 6, Jiefang Street, Dalian, 116001, China. ; Mingliang Du:1.Department of Cardiology, Dalian Municipal Central Hospital, Dalian, China. ; Qin Yu:1.Dalian Medical University, No. 9 West Section Lvshun South Road, Dalian, 116044, China. dalianyq0411@163.com. ; 2.Department of Cardiology, Affiliated Zhongshan Hospital of Dalian University, No. 6, Jiefang Street, Dalian, 116001, China. dalianyq0411@163.com. ;
期刊：circABCA3 knockdown relieves hypoxia-induced human cardiac microvascular endothelial cell dysfunction by targeting the miR-671-5p/PCSK9 axis
DOI：10.1007/s12192-023-01377-2
影响因子 ：3.8
引用产品： 人心脏微血管内皮细胞
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Association of circulating MtDNA with CVD in hemodialysis patients and in vitro effect of exogenous MtDNA on cardiac microvascular inflammation (2023-02-08)
作者：Zhen Fan:1.Department of Geriatrics, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan, China. ; Ya Feng:1.Department of Nephrology, Clinical Medical College, The First Affiliated Hospital of Chengdu Medical College, Chengdu, Sichuan, China. ; Li Zang:1.Department of Nephrology, Clinical Medical College, The First Affiliated Hospital of Chengdu Medical College, Chengdu, Sichuan, China. ; Yi Guo:1.Department of Neurology, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, No. 32, West Section 2, Yihuan Road, Qingyang District, Chengdu, 610072, Sichuan, China. 419535941@qq.com. ; Xiao-Yi Zhong:1.Department of Nephrology, Xinqiao Hospital, Army Medical University (Third Military Medical University), No. 83 Xinqiao Zhengjie, Shapingba District, Chongqing, 400037, China. zhongxy729@163.com. ;
期刊：Association of circulating MtDNA with CVD in hemodialysis patients and in vitro effect of exogenous MtDNA on cardiac microvascular inflammation
DOI：10.1186/s12872-023-03104-2
影响因子 ：2.1
引用产品： 人心脏微血管内皮细胞 , 人心脏微血管内皮细胞完全培养基
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Eucommia ulmoides extract attenuates angiotensin II-induced cardiac microvascular endothelial cell dysfunction by inactivating p53 (2023-06-26)
作者：Department of Pharmacy, Affiliated Hospital of Jinggangshan University, Ji’an, P.R. China
期刊：Eucommia ulmoides extract attenuates angiotensin II-induced cardiac microvascular endothelial cell dysfunction by inactivating p53
DOI：10.1590/s2175-97902023e22473
影响因子 ：1.3
引用产品： 人心脏微血管内皮细胞
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Mesoporous tantalum oxide nanomaterials induced cardiovascular endothelial cell apoptosis via mitochondrial-endoplasmic reticulum stress apoptotic pathway (2022-12-19)
作者：Yuanyong Jiao:1.Department of Vascular Surgery, The First Affiliated Hospital with Nanjing Medical University (Jiangsu Province Hospital), Nanjing, China. ; Xiwei Zhang:1.Department of Vascular Surgery, The First Affiliated Hospital with Nanjing Medical University (Jiangsu Province Hospital), Nanjing, China. ; Hongyu Yang:1.Department of Vascular Surgery, The First Affiliated Hospital with Nanjing Medical University (Jiangsu Province Hospital), Nanjing, China. ; Hao Ma:1.Department of Vascular Surgery, The First Affiliated Hospital with Nanjing Medical University (Jiangsu Province Hospital), Nanjing, China. ; Junjie Zou:1.Department of Vascular Surgery, The First Affiliated Hospital with Nanjing Medical University (Jiangsu Province Hospital), Nanjing, China. ;
期刊：Mesoporous tantalum oxide nanomaterials induced cardiovascular endothelial cell apoptosis via mitochondrial-endoplasmic reticulum stress apoptotic pathway
DOI：10.1080/10717544.2022.2147251
影响因子 ：6.8
引用产品： 人心脏微血管内皮细胞
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Donepezil ameliorates oxygen‑glucose deprivation/reoxygenation‑induced cardiac microvascular endothelial cell dysfunction through PARP1/NF‑κB signaling (2002-02-20)
作者：Yuan Li:1.Department of Cardiology, Rizhao Central Hospital, Rizhao, Shandong 276800, P.R. China. ; Xiuzhen Sun:1.Department of Cardiology, Rizhao Central Hospital, Rizhao, Shandong 276800, P.R. China. ; Jianqun Zhuang:1.Department of Cardiology, Rizhao Central Hospital, Rizhao, Shandong 276800, P.R. China. ; Junzhi Wang:1.Department of Cardiology, Chengyang People's Hospital of Qingdao, Qingdao, Shandong 266109, P.R. China. ; Chunqiang Yang:1.Department of Cardiology, Rizhao Central Hospital, Rizhao, Shandong 276800, P.R. China. ;
期刊：Donepezil ameliorates oxygen‑glucose deprivation/reoxygenation‑induced cardiac microvascular endothelial cell dysfunction through PARP1/NF‑κB signaling
影响因子 ：3.4
引用产品： 人心脏微血管内皮细胞
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Dexmedetomidine protects cardiac microvascular endothelial cells from the damage of ogd/r through regulation of the pparδ-mediated autophagy (2003-01-20)
作者：Qingbo Shao:1.Department of Anesthesiology, Minhang Branch, Zhongshan Hospital, Fudan University, Shanghai, China. ; Jing Xia:1.Shanghai International Travel Healthcare Center, Shanghai, China. ; Pinwen Wu:1.Department of Anesthesiology, Minhang Branch, Zhongshan Hospital, Fudan University, Shanghai, China. ; Jiazhou Ying:1.Department of Anesthesiology, Minhang Branch, Zhongshan Hospital, Fudan University, Shanghai, China. ;
期刊：Dexmedetomidine protects cardiac microvascular endothelial cells from the damage of ogd/r through regulation of the pparδ-mediated autophagy
影响因子 ：2.4
引用产品： 人心脏微血管内皮细胞
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乌司他丁对人心脏微循环内皮细胞释放炎性因子的作用 (2011-08-20)
作者：Department of Cardiothoracic Surgery, The First Affiliated Hospital of Hainan Medical College, Haikou 570102, China
期刊：乌司他丁对人心脏微循环内皮细胞释放炎性因子的作用
DOI：10.3969/j.issn.1003-6350.2018.22.001
影响因子 ：2.3
引用产品： 人心脏微血管内皮细胞
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