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产品概述
产品简介
人脂肪间充质干细胞成脂诱导分化培养基专门为人脂肪间充质干细胞成脂诱导分化而开发，针对人脂肪间充质干细胞的特性优化分化试剂的配方，可增加人脂肪间充质干细胞的成脂分化效果。
本产品含血清成分，仅用于科研用途，不可用于诊断、治疗、临床及其他用途。
成脂诱导分化培养基A液：
成分名称 | 添加体积 |
成脂分化专用基础培养基A Basal Medium For Stem Cells Adipogenic Differentiation A | 177 mL |
成脂分化专用胎牛血清（FBS） Fetal Bovine Serum For Stem Cells Adipogenic Differentiation | 20 mL |
人脂肪间充质干细胞成脂分化添加物A① Supplement For hADSCs Adipogenic Differentiation A① | 2.8 mL |
人脂肪间充质干细胞成脂分化添加物A② Supplement For hADSCs Adipogenic Differentiation A② | 200 μL |
成脂诱导分化培养基B液
成分名称 | 添加体积 |
成脂分化专用基础培养基B Basal Medium For Stem Cells Adipogenic Differentiation B | 177.2 mL |
成脂诱导分化专用胎牛血清（FBS） Fetal Bovine Serum For Stem Cells Adipogenic Differentiation | 20 mL |
人脂肪间充质干细胞成脂分化添加物B Supplement For hADSCs Adipogenic Differentiation B | 2.8 mL |
辅助试剂
成分名称 | 添加体积 |
油红O染色液 Oil Red O Solution | 10 mL |
明胶包被液 Gelatin Solution | 10 mL |
注：各成分请根据试剂管上标签标示温度保存。
产品说明
| 产品规格 | 400mL |
| 储存条件 | 按标签所示温度保存，避光储存 |
| 运输条件 | 冰袋冷藏运输 |
| 有效期 | 一年 |
注意事项
1.因为培养基的成分较多，请在配制过程中严格注意无菌操作。
2. A、B交替诱导是为了减轻A液中试剂对干细胞的影响，如果您的干细胞状态较好可在前7天先只使用A液进行刺激诱导（中途每2~3天更换新鲜A液），待脂滴快速出现后，再进行两种培养基交替诱导的操作。
3.血清离心：血清中可能会存在白色絮状沉淀物，这通常是胆固醇、脂肪酸酯以及一些蛋白质析出导致，属于正常现象，该现象不影响细胞的培养和诱导分化。若您欲去除这些絮状沉淀物，可以将血清分装至无菌离心管内，以400-600 g离心5 min，取上清液使用。但是我们不建议您以过滤的方法去除这些絮状沉淀物，一方面它可能会阻塞您的过滤膜；另一方面，过滤血清这种行为可能会导致血清中部分营养成分的流失。
参考文献
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Extracellular Vesicle-Loaded Oncogenic lncRNA NEAT1 from Adipose-Derived Mesenchymal Stem Cells Confers Gemcitabine Resistance in Pancreatic Cancer via miR-491-5p/Snail/SOCS3 Axis (2023-01-30)
作者：Rongxiang Wu:1.Jiangsu University, Zhenjiang 212013, China. ; Zhan Su:1.Department of Hepatopancreatobiliary Surgery, Xuzhou Cancer Hospital, Xuzhou 221005, China. ; Le Zhao:1.Department of Hepatopancreatobiliary Surgery, Xuzhou Cancer Hospital, Xuzhou 221005, China. ; Ruifeng Pei:1.Department of Hepatopancreatobiliary Surgery, Xuzhou Cancer Hospital, Xuzhou 221005, China. ; Yiren Ding:1.Department of Hepatopancreatobiliary Surgery, Xuzhou Cancer Hospital, Xuzhou 221005, China. ; Deqiang Li:1.Department of Hepatopancreatobiliary Surgery, Xuzhou Cancer Hospital, Xuzhou 221005, China. ; Shuo Zhu:1.Department of Hepatopancreatobiliary Surgery, Xuzhou Cancer Hospital, Xuzhou 221005, China. ; Lu Xu:1.Department of Hepatopancreatobiliary Surgery, Xuzhou Cancer Hospital, Xuzhou 221005, China. ; Wei Zhao:1.Department of Hepatopancreatobiliary Surgery, Xuzhou Cancer Hospital, Xuzhou 221005, China. ; Wuyuan Zhou:1.Department of Hepatopancreatobiliary Surgery, Xuzhou Cancer Hospital, Xuz
期刊：Extracellular Vesicle-Loaded Oncogenic lncRNA NEAT1 from Adipose-Derived Mesenchymal Stem Cells Confers Gemcitabine Resistance in Pancreatic Cancer via miR-491-5p/Snail/SOCS3 Axis
影响因子 ：4.3
引用产品： 人脂肪间充质干细胞成脂诱导培养基
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Hypoxia activates the PI3K/AKT/HIF-1α pathway to promote the anti-inflammatory effect of adipose mesenchymal stem cells (2023-05-01)
作者：Hongjing Ren:1.Southwest Medical University, NO.1 Section 1, Xianglin Road, Luzhou City, Sichuan Province 646000, China. ; Mengchang Liu:1.Southwest Medical University, NO.1 Section 1, Xianglin Road, Luzhou City, Sichuan Province 646000, China. ; Yueda Jihu:1.Southwest Medical University, NO.1 Section 1, Xianglin Road, Luzhou City, Sichuan Province 646000, China. ; Huizhen Zeng:1.Southwest Medical University, NO.1 Section 1, Xianglin Road, Luzhou City, Sichuan Province 646000, China. ; Chong Yao:1.Southwest Medical University, NO.1 Section 1, Xianglin Road, Luzhou City, Sichuan Province 646000, China. ; Hong Yan:1.Department of Plastic and Burn Surgery, Affiliated Hospital of Southwest Medical University, National Key Clinical Construction Specialty, Wound Repair and Regeneration Laboratory, NO.25 Taiping Street, Jiangyang District, Luzhou 646000 Sichuan Province, China. Electronic address: zxsswk@163.com. ;
期刊：Hypoxia activates the PI3K/AKT/HIF-1α pathway to promote the anti-inflammatory effect of adipose mesenchymal stem cells
DOI：10.1016/j.acthis.2023.152042
影响因子 ：2.5
引用产品： 人脂肪间充质干细胞成脂诱导培养基 , 人脂肪间充质干细胞成骨诱导分化培养基
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MiR-3064 in Epicardial Adipose-Derived Exosomes Targets Neuronatin to Regulate Adipogenic Differentiation of Epicardial Adipose Stem Cells (2008-01-20)
作者：Wenkai Yang:1.Department of Cardiovascular Surgery, Central People's Hospital of Zhanjiang, Zhanjiang, China. ; Hanjian Tu:1.Department of Cardiac Surgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China. ; Kai Tang:1.Department of Cardiovascular Surgery, Central People's Hospital of Zhanjiang, Zhanjiang, China. ; Haozhong Huang:1.Department of Cardiovascular Surgery, Central People's Hospital of Zhanjiang, Zhanjiang, China. ; Shi Ou:1.Department of Cardiovascular Surgery, Central People's Hospital of Zhanjiang, Zhanjiang, China. ; Jianguo Wu:1.Department of Cardiovascular Surgery, Central People's Hospital of Zhanjiang, Zhanjiang, China. ;
期刊：MiR-3064 in Epicardial Adipose-Derived Exosomes Targets Neuronatin to Regulate Adipogenic Differentiation of Epicardial Adipose Stem Cells
影响因子 ：3.6
引用产品： 人脂肪间充质干细胞成脂诱导培养基
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