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小鼠结肠黏膜上皮细胞
Mouse Colonic Mucosal Epithelial Cells
货号：CP-M159
规格：5×10⁵Cells/T25培养瓶
价格： ￥3380
培养基货号： CM-M159
产品概述
小鼠结肠黏膜上皮细胞
Cat NO.: CP-M159
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产品名称：小鼠结肠黏膜上皮细胞
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组织来源：结肠组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
小鼠结肠黏膜上皮细胞分离自结肠组织；结肠在右髂窝内续于盲肠，在第3骶椎平面连接直肠。结肠分升结肠、横结肠、降结肠和乙状结肠4部分，大部分固定于腹后壁，结肠的排列酷似英文字母“M”，将小肠包围在内。结肠横切面由内到外依次为：黏膜(上皮层、固有层、黏膜肌层)，黏膜下层(疏松结缔组织)，肌层(内环形、外纵行两层平滑肌)，外膜(纤维膜或浆膜)。肠黏膜上皮细胞是机体内外环境的重要屏障，持续暴露于大量抗原中，也是机体面对病原微生物的第一道防线。因此，肠黏膜上皮细胞除有吸收、分泌和转运等重要生理功能之外，在黏膜先天性和获得性免疫防御机制中也起着重要作用。肠黏膜上皮细胞作为首先接触抗原的细胞，在黏膜免疫反应的起始阶段发挥关键作用，它决定黏膜免疫反应的发生、性质和强度。探讨正常结肠黏膜上皮细胞的分离、体外培养方法，为研究肠黏膜上皮细胞以及与肠黏膜相关疾病建立合适的细胞模型。
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方法简介：
普诺赛实验室分离的小鼠结肠黏膜上皮细胞采用先机械分离法、后胶原酶消化法并通过上皮细胞专用培养基培养筛选制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的小鼠结肠黏膜上皮细胞经Cytokeratin-18免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 鼠尾胶原Ⅰ(2-5μg/cm2) 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-M159 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 上皮细胞样 传代特性 可传1-2代 消化液 0.25%胰蛋白酶 小鼠结肠黏膜上皮细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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Transcription suppression of GABARAP mediated by lncRNA XIST-EZH2 interaction triggers caspase-11-dependent inflammatory injury in ulcerative colitis (2024-03-06)
作者：Dan Gu:1.Department of Gastroenterology, The Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, Hengyang 421001, Hunan, PR China. ; Ting Cao:1.Department of Gastroenterology, The Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, Hengyang 421001, Hunan, PR China. ; Shijie Yi:1.Department of Gastrointestinal Surgery, The Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, Hengyang 421001, Hunan, PR China. ; Xiaoqian Li:1.Department of Gastroenterology, The Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, Hengyang 421001, Hunan, PR China. ; Ya Liu:1.Department of Anorectal Surgery, The Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, Hengyang 421001, Hunan, PR China. Electronic address: dr_liuya3201@163.com. ;
期刊：Transcription suppression of GABARAP mediated by lncRNA XIST-EZH2 interaction triggers caspase-11-dependent inflammatory injury in ulcerative colitis
DOI：10.1016/j.imbio.2024.152796
影响因子 ：2.8
引用产品： 小鼠结肠黏膜上皮细胞 , 小鼠结肠黏膜上皮细胞完全培养基
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Ginsenoside Rg3 Ameliorates DSS-Induced Colitis by Inhibiting NLRP3 Inflammasome Activation and Regulating Microbial Homeostasis (2023-02-08)
作者：Dongcai Liu:1.General Surgery Department, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China. ; Qingquan Tian:1.General Surgery Department FIVE, People's Hospital of Xiangxi Tujia and Miao Autonomous Prefecture, Jishou, Hunan 416000, China. ; Kuijie Liu:1.General Surgery Department, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China. ; Feng Ren:1.General Surgery Department, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China. ; Ganglei Liu:1.General Surgery Department, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China. ; Jingyu Zhou:1.General Surgery Department, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China. ; Lianwen Yuan:1.General Surgery Department, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China. ; Zhixue Fang:1.Hunan Provincial People's Hospital, The First Affiliate
期刊：Ginsenoside Rg3 Ameliorates DSS-Induced Colitis by Inhibiting NLRP3 Inflammasome Activation and Regulating Microbial Homeostasis
影响因子 ：6.1
引用产品： 小鼠骨髓来源巨噬细胞 , 小鼠结肠黏膜上皮细胞
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A signal peptide derived from Hsp60 induces protective cytotoxic T lymphocyte immunity against lymphoid malignancies independently of TAP and classical MHC-I (2008-00-20)
作者：Xun-Rui Chen:1.Oncology Department, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 201900, China. ; Hai-Hua Yuan:1.Oncology Department, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 201900, China. ; Jia-Hui Guo:1.Oncology Department, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 201900, China. ; Wen-Ying Zhang:1.Oncology Department, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 201900, China. ; Qian-Qian Li:1.Oncology Department, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 201900, China. ; Guo-Ding Huang:1.Oncology Department, Hainan Western Central Hospital, Danzhou, 571700, Hainan Province, China. ; Yan-Jie Zhang:1.Oncology Department, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 201900, China. Elec
期刊：A signal peptide derived from Hsp60 induces protective cytotoxic T lymphocyte immunity against lymphoid malignancies independently of TAP and classical MHC-I
DOI：10.1016/j.canlet.2020.08.016
影响因子 ：9.7
引用产品： 小鼠支气管上皮细胞 , 小鼠结肠黏膜上皮细胞 , 小鼠心肌细胞 , 小鼠胰岛细胞
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