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小鼠脑微血管内皮细胞
Mouse Brain Microvascular Endothelial Cells
货号：CP-M108
规格：5×10⁵Cells/T25培养瓶
价格： ￥4380
培养基货号： CM-M108
产品概述
小鼠脑微血管内皮细胞
Cat NO.: CP-M108
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产品名称：小鼠脑微血管内皮细胞
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英文简称：BMVECs
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组织来源：脑组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
小鼠脑微血管内皮细胞分离自脑组织；脑微血管内皮细胞是血脑屏障的主要组成成分，它是组成脑微血管腔面单层扁平上皮样细胞，能够限制可溶性物质和细胞等从血液进入大脑，大脑微血管内皮细胞与外周内皮细胞相比具有一些相同特性。它所产生和分泌的生物活性物质对维持血管张力、调节血压、抗血栓形成等有重要作用，在脑血管疾病的发病机制中有重要病理生理学意义。与外周内皮细胞相同，大脑微血管内皮细胞表面表达细胞粘附分子，调控白细胞进入大脑。由于微血管内皮细胞的器官特异性，内皮细胞通常取源于疾病研究的相关组织。其主要特征如下：①脑微血管内皮细胞存在许多细胞间紧密连接，产生很高的跨内皮阻抗，延迟细胞旁的通量；②脑微血管内皮细胞缺乏内皮细胞的窗孔结构，其液相物质胞饮水平较低；③脑微血管内皮细胞具有不对称定位酶和载体介导转运系统，从而产生“两极分化”的表现型。
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方法简介：
普诺赛实验室分离的小鼠脑微血管内皮细胞采用胶原酶-中性蛋白酶混合消化法结合密度梯度离心法、最后通过内皮细胞专用培养基培养筛选制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的小鼠脑微血管内皮细胞经CD31免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 PLL（0.1mg/ml）或明胶（0.1%） 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-M108 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 内皮细胞样 传代特性 可传1-2代；不建议多次传代 消化液 0.25%胰蛋白酶 小鼠脑微血管内皮细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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Advanced Glycation End-Products (AGEs) Promote Endothelial Cell Pyroptosis Under Cerebral Ischemia and Hypoxia via HIF-1α-RAGE-NLRP3 (2023-01-18)
作者：Chenyang Han:1.The Second Affiliated Hospital of Jiaxing University, Jiaxing, China. ; Liping Zhai:1.The Second Affiliated Hospital of Jiaxing University, Jiaxing, China. ; Heping Shen:1.The Second Affiliated Hospital of Jiaxing University, Jiaxing, China. ; Jin Wang:1.The Second Affiliated Hospital of Jiaxing University, Jiaxing, China. ; Qiaobing Guan:1.The Second Affiliated Hospital of Jiaxing University, Jiaxing, China. guanqb@126.com. ;
期刊：Advanced Glycation End-Products (AGEs) Promote Endothelial Cell Pyroptosis Under Cerebral Ischemia and Hypoxia via HIF-1α-RAGE-NLRP3
DOI：10.1007/s12035-023-03228-8
影响因子 ：5.1
引用产品： 小鼠脑微血管内皮细胞 , bEnd.3 [BEND3] 细胞
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Nano-integrated cascade antioxidases opsonized by albumin bypass the blood–brain barrier for treatment of ischemia-reperfusion injury (2022-10-17)
作者：Wuxuan Wang:1.Stroke Center & Department of Neurology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230036, China. xfliu2@ustc.edu.cn. ; Zheng Zhang:1.Division of Molecular Medicine, Hefei National Laboratory for Physical Sciences at Microscale, The CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Life Sciences University of Science and Technology of China, Hefei, Anhui 230027, China. yucaiwang@ustc.edu.cn. ; 2.School of Biomedical Sciences and Engineering, South China University of Technology, Guangzhou International Campus, Guangzhou 511442, China. ; Yi Liu:1.Division of Molecular Medicine, Hefei National Laboratory for Physical Sciences at Microscale, The CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Life Sciences University of Science and Technology of China, Hefei, Anhui 230027, China. yucaiwang@ustc.edu.cn. ; Lingqi Kong:1.Stroke Center & Dep
期刊：Nano-integrated cascade antioxidases opsonized by albumin bypass the blood–brain barrier for treatment of ischemia-reperfusion injury
影响因子 ：7.6
引用产品： HT22 细胞 , PC-12 (High differentiation) 细胞 , 小鼠脑微血管内皮细胞
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SIRT1/FOXO1 Axis-Mediated Hippocampal Angiogenesis is Involved in the Antidepressant Effect of Chaihu Shugan San (2022-08-23)
作者：Shan Zhang:1.Department of Traditional Chinese Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, People's Republic of China. ; Yujia Lu:1.Department of Traditional Chinese Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, People's Republic of China. ; Wei Shi:1.Department of Traditional Chinese Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, People's Republic of China. ; Yi Ren:1.Department of Traditional Chinese Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, People's Republic of China. ; Kaihui Xiao:1.Department of Traditional Chinese Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, People's Republic of China. ; Wei Chen:1.Experimental and Translational Research Center, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, People's Republic of China. ; Li Li:1.Department of Traditional
期刊：SIRT1/FOXO1 Axis-Mediated Hippocampal Angiogenesis is Involved in the Antidepressant Effect of Chaihu Shugan San
影响因子 ：4.3
引用产品： 小鼠脑微血管内皮细胞
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