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![bEnd.3 [BEND3] (小鼠脑微血管内皮细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0598-1.jpg)
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产品概述
| 名称 | bEnd.3 [BEND3] (小鼠脑微血管内皮细胞) (STR鉴定正确) |
| 别称 | bEND.3; b.End3; Bend.3; bEnd3; brain-derived Endothelial cells.3 |
| 种属 | 小鼠 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:2-1:4 |
| 推荐换液频率 | 2-3次/周 |
| 注意事项 | 1.该细胞在低密度下形态不规则，细胞数量少，铺展面积大，高密度后形态会趋于一致，胞体密集；2.细胞增殖偏慢，初次传代建议1:2，细胞稳定后建议传代比例也不超过1:4；3.注意观察培养基颜色，颜色偏紫会导致细胞漂浮增加；4.消化时间会随着细胞生长时间和细胞密度而有所不同，需要结合消化时细胞状态来判断，消化到细胞可以滑落方可终止。 |
| 背景描述 | The cells were transformed by infection with the NTKmT retrovirus vector that expresses polyomavirus middle T antigen |
| 年龄（性别） | 6周龄 |
| 组织来源 | 大脑皮层 |
| 细胞类型 | 转化细胞系 |
| 生物安全等级 | BSL-1 |
| 抗原表达情况 | ICAM-1 +; VCAM-1 +; MAdCAM-1 + |
| 基因表达情况 | von Willebrand factor,ICAM-1 +; VCAM-1 +; MAdCAM-1 +,The endothelial nature of these cells was confirmed by the observed expression of von Willebrand factor and uptake of fluorescently labeled low density lipoprotein (LDL).,The expression of Peyer's Patch high endothelial receptor for lymphocytes, the mucosal vascular addressin (MAdCAM-1) and E-selectin can be induced on bEnd |
| 保藏机构 | ATCC; CRL-2299 BCRC; 60515 ECACC; 96091929 |
STR鉴定
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STR位点信息
18-3 18 6-7 11,12 5-5 14 X-1 26 1-2 13 7-1 29 8-1 16 1-1 10 19-2 13 15-3 19.3,20.3 12-1 17 6-4 17 4-2 18.3 3-2 14 2-1 9 13-1 16.2
11-2 14 17-2 12 -
STR鉴定图
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参考文献
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Kdm6a-CNN1 axis orchestrates epigenetic control of trauma-induced spinal cord microvascular endothelial cell senescence to balance neuroinflammation for improved neurological repair (2024-03-25)
期刊：Bone Research
DOI：10.1038/s41413-024-00323-x
影响因子 ：12.7
引用产品： bEnd.3 [BEND3] 细胞
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Enhancing Neuroprotection in Mouse Model of Parkinson’s Disease through Protein Nanosystem Conjugation with ApoE Peptide for miR-124 Delivery (2024-02-12)
期刊：ACS Applied Materials & Interfaces
影响因子 ：9.5
引用产品： bEnd.3 [BEND3] 细胞
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The Dual Angiogenesis Effects via Nrf2/HO-1 Signaling Pathway of Melatonin Nanocomposite Scaffold on Promoting Diabetic Bone Defect Repair (2024-03-15)
期刊：International Journal of Nanomedicine
影响因子 ：8.0
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Canagliflozin protects against hyperglycemia-induced cerebrovascular injury by preventing blood-brain barrier (BBB) disruption via AMPK/Sp1/adenosine A2A receptor (2024-02-09)
期刊：EUROPEAN JOURNAL OF PHARMACOLOGY
DOI：10.1016/j.ejphar.2024.176381
影响因子 ：5.0
引用产品： bEnd.3 [BEND3] 细胞
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A novel study of brain microvascular endothelial cells induced by astrocyte conditioned medium for constructing blood brain barrier model in vitro: A promising tool for meningitis of teleost (2024-01-23)
期刊：FISH & SHELLFISH IMMUNOLOGY
影响因子 ：4.7
引用产品： bEnd.3 [BEND3] 细胞
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Inhibition of piezo1 prevents chronic cerebral hypoperfusion-induced cognitive impairment and blood brain barrier disruption (2024-02-22)
期刊：NEUROCHEMISTRY INTERNATIONAL
DOI：10.1016/j.neuint.2024.105702
影响因子 ：4.2
引用产品： bEnd.3 [BEND3] 细胞
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Long noncoding RNA KCNQ1OT1 aggravates cerebral infarction by regulating PTBT1/SIRT1 via miR-16-5p (2024-02-07)
期刊：JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY
影响因子 ：3.2
引用产品： bEnd.3 [BEND3] 细胞
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Targeted Delivery of RGD-CD146+CD271+ Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes Promotes Blood–Spinal Cord Barrier Repair after Spinal Cord Injury (2023-09-11)
期刊：ACS Nano
影响因子 ：17.1
引用产品： bEnd.3 [BEND3] 细胞
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C3N nanodots inhibits Aβ peptides aggregation pathogenic path in Alzheimer’s disease (2023-09-15)
期刊：Nature Communications
DOI：10.1038/s41467-023-41489-y
影响因子 ：16.6
引用产品： bEnd.3 [BEND3] 细胞 , BV2 细胞
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Protection of a novel velvet antler polypeptide PNP1 against cerebral ischemia-reperfusion injury (2023-07-13)
期刊：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI：10.1016/j.ijbiomac.2023.125815
影响因子 ：8.2
引用产品： bEnd.3 [BEND3] 细胞 , BV2 细胞
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Active fraction of Polyrhachis vicina (Roger) alleviated cerebral ischemia/reperfusion injury by targeting SIRT3-mediated mitophagy and angiogenesis (2023-09-18)
期刊：PHYTOMEDICINE
DOI：10.1016/j.phymed.2023.155104
影响因子 ：7.9
引用产品： bEnd.3 [BEND3] 细胞
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Elabela-APJ axis mediates angiogenesis via YAP/TAZ pathway in cerebral ischemia/reperfusion injury (2023-02-21)
期刊：Translational Research
DOI：10.1016/j.trsl.2023.02.002
影响因子 ：7.8
引用产品： bEnd.3 [BEND3] 细胞
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A Novel Blood–Brain Barrier-Penetrating and Vascular-Targeting Chimeric Peptide Inhibits Glioma Angiogenesis (2023-05-15)
期刊：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
影响因子 ：5.6
引用产品： bEnd.3 [BEND3] 细胞
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MCC950 alleviates seizure severity and angiogenesis by inhibiting NLRP3/ IL-1β signaling pathway-mediated pyroptosis in mouse model of epilepsy (2023-11-30)
期刊：INTERNATIONAL IMMUNOPHARMACOLOGY
DOI：10.1016/j.intimp.2023.111236
影响因子 ：5.6
引用产品： BV2 细胞 , bEnd.3 [BEND3] 细胞
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Mechanism of neocryptotanshinone in protecting against cerebral ischemic injury: By suppressing M1 polarization of microglial cells and promoting cerebral angiogenesis (2023-02-09)
期刊：INTERNATIONAL IMMUNOPHARMACOLOGY
DOI：10.1016/j.intimp.2023.109815
影响因子 ：5.6
引用产品： BV2 细胞 , bEnd.3 [BEND3] 细胞
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USP14 inhibition promotes recovery by protecting BBB integrity and attenuating neuroinflammation in MCAO mice (2023-06-02)
期刊：CNS Neuroscience & Therapeutics
影响因子 ：5.5
引用产品： bEnd.3 [BEND3] 细胞
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Inhibition of HDAC6 promotes microvascular endothelial cells to phagocytize myelin debris and reduces inflammatory response to accelerate the repair of spinal cord injury (2023-08-29)
期刊：CNS Neuroscience & Therapeutics
影响因子 ：5.5
引用产品： bEnd.3 [BEND3] 细胞 , RAW 264.7 细胞
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Advanced Glycation End-Products (AGEs) Promote Endothelial Cell Pyroptosis Under Cerebral Ischemia and Hypoxia via HIF-1α-RAGE-NLRP3 (2023-01-18)
期刊：MOLECULAR NEUROBIOLOGY
DOI：10.1007/s12035-023-03228-8
影响因子 ：5.1
引用产品： 小鼠脑微血管内皮细胞 , bEnd.3 [BEND3] 细胞
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Nuclear Factor Erythroid 2-Related Factor 2 is Essential for Low-Normobaric Oxygen Treatment-Mediated Blood-Brain Barrier Protection Following Ischemic Stroke (2023-11-11)
期刊：MOLECULAR NEUROBIOLOGY
DOI：10.1007/s12035-023-03767-0
影响因子 ：5.1
引用产品： bEnd.3 [BEND3] 细胞
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GPR124 induces NLRP3 inflammasome-mediated pyroptosis in endothelial cells during ischemic injury (2023-12-01)
期刊：EUROPEAN JOURNAL OF PHARMACOLOGY
DOI：10.1016/j.ejphar.2023.176228
影响因子 ：5.0
引用产品： bEnd.3 [BEND3] 细胞
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Overexpression of ACE2 ameliorates Aβ-induced blood–brain barrier damage and angiogenesis by inhibiting NF-κB/VEGF/VEGFR2 pathway (2023-05-14)
期刊：Animal Models and Experimental Medicine
影响因子 ：3.7
引用产品： bEnd.3 [BEND3] 细胞
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Vascular endothelial cell-derived exosomal miR-1246 facilitates posterior capsule opacification development by targeting GSK-3β in diabetes mellitus (2023-04-10)
期刊：EXPERIMENTAL EYE RESEARCH
DOI：10.1016/j.exer.2023.109463
影响因子 ：3.4
引用产品： bEnd.3 [BEND3] 细胞
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Specific anti-glioma targeted-delivery strategy of engineered small extracellular vesicles dual-functionalised by Angiopep-2 and TAT peptides (2022-08-06)
期刊：Journal of Extracellular Vesicles
影响因子 ：17.3
引用产品： bEnd.3 [BEND3] 细胞 , U-87 MG 细胞 , 293T [HEK-293T] 细胞 , A549 [A-549] 细胞 , SH-SY5Y [SHSY-5Y] 细胞 , Hep G2 细胞
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Gold nanocluster & indocyanine green based triple-effective therapy for MRSA infected central nervous system (2004-02-20)
期刊：Applied Materials Today
DOI：10.1016/j.apmt.2022.101453
影响因子 ：8.3
引用产品： NCTC clone 929 [L 细胞, L-929] 细胞 , BV2 细胞 , bEnd.3 [BEND3] 细胞 , PUMC-HUVEC-T1
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Endothelial deletion of TBK1 contributes to BRB dysfunction via CXCR4 phosphorylation suppression (2022-10-28)
期刊：Cell Death Discovery
DOI：10.1038/s41420-022-01222-y
影响因子 ：7.1
引用产品： bEnd.3 [BEND3] 细胞 , 人视网膜微血管内皮细胞 , bEnd.3 [BEND3]细胞专用培养基 , 人视网膜微血管内皮细胞完全培养基
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Biosynthetic Gas Vesicles Combined with Focused Ultrasound for Blood–Brain Barrier Opening (2022-12-28)
期刊：International Journal of Nanomedicine
影响因子 ：7.0
引用产品： bEnd.3 [BEND3] 细胞
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A bone-targeted engineered exosome platform delivering siRNA to treat osteoporosis (2009-01-20)
期刊：Bioactive Materials
DOI：10.1016/j.bioactmat.2021.09.015
影响因子 ：18.9
引用产品： MC3T3-E1 Subclone 14 细胞 , bEnd.3 [BEND3] 细胞 , RAW 264.7 细胞
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Design and screening of a novel neuropilin-1 targeted penetrating peptide for anti-angiogenic therapy in glioma (2001-01-20)
期刊：Life Sciences
影响因子 ：6.1
引用产品： bEnd.3 [BEND3] 细胞
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Nicotinamide mononucleotide (NMN) protects bEnd.3 cells against H2O2‐induced damage via NAMPT and the NF‐κB p65 signalling pathway (2002-01-20)
期刊：FEBS Open Bio
影响因子 ：2.6
引用产品： bEnd.3 [BEND3] 细胞
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The functional curcumin liposomes induce apoptosis in C6 glioblastoma cells and C6 glioblastoma stem cells in vitro and in animals (2002-07-20)
期刊：International Journal of Nanomedicine
影响因子 ：8.0
引用产品： bEnd.3 [BEND3] 细胞
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