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产品概述
小鼠视网膜Muller细胞
Cat NO.: CP-M117
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产品名称：小鼠视网膜Muller细胞
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组织来源：视网膜组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
小鼠视网膜Muller细胞分离自视网膜组织；视网膜居于眼球壁的内层，是一层透明的薄膜。视网膜由色素上皮层和视网膜感觉层组成，两层间在病理情况下可分开，称为视网膜脱离。色素上皮层与脉络膜紧密相连，由色素上皮细胞组成，它们具有支持和营养光感受器细胞、遮光、散热以及再生和修复等作用。组织学上视网膜分为10层，由外向内分别为：色素上皮层、视锥、视杆细胞层、外界膜、外颗粒层、外丛状层、内颗粒层、内丛状层、神经节细胞层、神经纤维层、内界膜。视网膜内层为衬于血管膜内面的一层薄膜，有感光作用；后部鼻侧有一视神经乳头。视网膜上的感觉层是由三个神经元组成。第一神经元是视细胞层，专司感光，它包括锥细胞和杆细胞。视杆细胞主要在离中心凹较远的视网膜上，而视锥细胞则在中心凹处最多。第二层叫双节细胞，约有10到数百个视细胞通过双节细胞与一个神经节细胞相联系，负责联络作用。第三层叫节细胞层，专管传导。视网膜是一层菲薄的但又非常复杂的结构，它贴于眼球的后壁部，传递来自视网膜感受器冲动的神经纤维跨越视网膜表面，经由视神经到达出口。视网膜的分辨力是不均匀的，在黄斑区，其分辨能力最强。视网膜Muller细胞作为视网膜中的主要胶质细胞，大约占视网膜胶质细胞的90%，因而在视网膜疾病中起着何种作用也受到越来越多的关注。在超微结构水平上，Muller细胞的胞质似乎比临近的其他细胞更高的电子密度，更发达的内质网，细胞核是典型的卵圆形或多角形。但在不同物种中或同一物种中的不同部位，Muller细胞形态也会有所差异的。视网膜Muller细胞是一种特化的神经胶质细胞，不仅具有维持视网膜的正常结构和功能的作用，并调制视网膜神经元活动，还能参与多种病理过程，尤其是眼底增殖性病变，如增生性玻璃体视网膜病变、增生性糖尿病视网膜病变等，体外培养Muller细胞是研究这些增殖性病变途径之一。
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方法简介：
普诺赛实验室分离的小鼠视网膜Muller细胞采用胶原酶消化法和胰蛋白酶反复消化法制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的小鼠视网膜Muller细胞经GS免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-M117 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 梭形、多角形 传代特性 可传3代左右 消化液 0.25%胰蛋白酶 小鼠视网膜Muller细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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LncRNA MALAT1 aggravates the retinal angiogenesis via miR-320a/HIF-1α axis in diabetic retinopathy (2002-02-20)
作者：Zhen Chen:1.Department of Ophthalmology, The First People's Hospital of Yunnan Province, Kunming, Yunnan, 650032, China; Medical College of Kunming University of Science and Technology, Kunming, Yunnan, 650500, China; Medical College of Dali University, Dali, Yunnan, 671003, China. ; Jingying Yang:1.Department of Ophthalmology, The First People's Hospital of Yunnan Province, Kunming, Yunnan, 650032, China. ; Yang Gao:1.Department of Ophthalmology, The First People's Hospital of Yunnan Province, Kunming, Yunnan, 650032, China. ; Shanshan Jiang:1.Department of Ophthalmology, The First People's Hospital of Yunnan Province, Kunming, Yunnan, 650032, China; Medical College of Dali University, Dali, Yunnan, 671003, China. ; Zuyou Li:1.Department of Ophthalmology, The First People's Hospital of Yunnan Province, Kunming, Yunnan, 650032, China; Medical College of Kunming University of Science and Technology, Kunming, Yunnan, 650500, China. ; Yao Wang:1.Department of Ophthalmology, The First
期刊：LncRNA MALAT1 aggravates the retinal angiogenesis via miR-320a/HIF-1α axis in diabetic retinopathy
DOI：10.1016/j.exer.2022.108984
影响因子 ：3.4
引用产品： 小鼠视网膜Muller细胞 , 小鼠视网膜微血管内皮细胞 , 293T [HEK-293T] 细胞 , DMEM/F12完全培养基 (含10%FBS) , DMEM高糖完全培养基 (含10%FBS)
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Berberine protects against diabetic retinopathy by inhibiting cell apoptosis via deactivation of the NF‑κB signaling pathway (2009-00-20)
作者：Jiajia Zhai:1.Department of Endocrinology, Xijing Hospital, Air Force Medical University, Xi'an, Shaanxi 710032, P.R. China. ; Zeping Li:1.Department of Clinical Medicine, School of Queen Mary, Nanchang University, Nanchang, Jiangxi 330031, P.R. China. ; Huifeng Zhang:1.Department of Neurology, Xi'an Electric Power Central Hospital, Xi'an, Shaanxi 710032, P.R. China. ; Louyan Ma:1.Department of Geratology, Xi'an Ninth Hospital, Xi'an, Shaanxi 710054, P.R. China. ; Zhengquan Ma:1.Department of Geratology, Xi'an Ninth Hospital, Xi'an, Shaanxi 710054, P.R. China. ; Yi Zhang:1.Department of Endocrinology, Xi'an Ninth Hospital, Xi'an, Shaanxi 710054, P.R. China. ; Jian Zou:1.Department of Internal Medicine, 522nd Hospital of Chinese People's Liberation Army, Luoyang, Henan 471003, P.R. China. ; Mo Li:1.Department of Geratology, Xi'an Ninth Hospital, Xi'an, Shaanxi 710054, P.R. China. ; Li Ma:1.Department of Geratology, Xi'an Ninth Hospital, Xi'an, Shaanxi 710054, P.R. China. ; Xin
期刊：Berberine protects against diabetic retinopathy by inhibiting cell apoptosis via deactivation of the NF‑κB signaling pathway
影响因子 ：3.4
引用产品： 小鼠视网膜Muller细胞 , 小鼠视网膜Muller细胞完全培养基
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Müller Cell Regulated Microglial Activation and Migration in Rats With N-Methyl-N-Nitrosourea-Induced Retinal Degeneration (2012-08-20)
作者：Shuai Zhang:1.Department of Anatomy, Histology and Embryology, School of Basic Medical Sciences, Fudan University, Shanghai, China. ; Shanshan Zhang:1.Department of Anatomy, Histology and Embryology, School of Basic Medical Sciences, Fudan University, Shanghai, China. ; Wenqing Gong:1.Department of Anatomy, Histology and Embryology, School of Basic Medical Sciences, Fudan University, Shanghai, China. ; Guopei Zhu:1.Department of Integrative Medicine and Neurobiology, School of Basic Medical Sciences, Shanghai, China. ; Songtao Wang:1.Department of Anatomy, Histology and Embryology, School of Basic Medical Sciences, Fudan University, Shanghai, China. ; Yalin Wang:1.Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, China. ; Michael Halim:1.Department of Anatomy, Histology and Embryology, School of Basic Medical Sciences, Fudan University, Shanghai, China. ; Kaidi Wang:1.Department of Radiation Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong U
期刊：Müller Cell Regulated Microglial Activation and Migration in Rats With N-Methyl-N-Nitrosourea-Induced Retinal Degeneration
影响因子 ：4.3
引用产品： 小鼠视网膜Muller细胞
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