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产品概述
产品简介
Dulbecco's改良培养基——DMEM(Dulbecco's Modified Eagle Medium)是在MEM培养基的基础上研制的,与MEM培养基相比,氨基酸的含量增加了2倍,维生素的含量增加了4倍,同时还增加了非必须氨基酸、微量铁离子以及丙酮酸钠。DMEM培养基维生素的含量增加了4倍,同时还增加了非必须氨基酸、微量铁离子以及丙酮酸钠。DMEM培养基最初设计葡萄糖含量为1000mg/L(低糖型),后来又发展出葡萄糖含量为4500mg/L(高糖型),现已广泛应用于各种细胞的培养。DMEM高糖型培养基普遍应用于生长快、粘附性低的细胞、杂交瘤的骨髓瘤细胞、克隆细胞、DNA转染的转化细胞、各种原代病毒宿主细胞、单一细胞的培养以及疫苗的生产,例如利用CHO细胞表达EPO和生产乙肝疫苗。
产品说明
产品形态 | 液体 |
产品浓度 | 1× |
产品规格 | 125mL×4 |
培养基成分 | DMEM高糖(PM150210)+10% FBS(164210-50)+1% P/S(PB180120)+1% GlutaMax(PB180419) |
细菌检测 | 阴性 |
真菌检测 | 阴性 |
支原体检测 | 阴性 |
细胞生长实验 | 细胞生长良好,形态正常 |
内毒素含量(EU/mL) | ≤3 |
储存条件 | 2-8℃,避光储存 |
运输条件 | 冰袋冷藏运输 |
有效期 | 6个月 |
注意事项
参考文献
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Anti-Cancer Potency of Copper-Doped Carbon Quantum Dots Against Breast Cancer Progression (2024-02-26)
期刊:International Journal of Nanomedicine
影响因子 :8.0
引用产品: NRK-52E 细胞 , TM3 细胞 , MA-782细胞专用培养基 , TM3细胞专用培养基 , DMEM高糖完全培养基 (含10%FBS)
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MiR-205-5p-mediated MAGI1 inhibition attenuates the injury induced by diabetic nephropathy (2024-02-07)
期刊:PHARMACOLOGY
影响因子 :3.1
引用产品: HRMC 细胞 , DMEM高糖完全培养基 (含10%FBS)
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Hyperthermia-triggered biomimetic bubble nanomachines (2023-08-11)
期刊:Nature Communications
DOI:10.1038/s41467-023-40474-9
影响因子 :16.6
引用产品: DMEM高糖完全培养基 (含10%FBS) , 4T1 细胞 , RPMI-1640 培养基
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Protective effect of sodium butyrate on intestinal barrier damage and uric acid reduction in hyperuricemia mice (2023-03-20)
期刊:BIOMEDICINE & PHARMACOTHERAPY
DOI:10.1016/j.biopha.2023.114568
影响因子 :7.5
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A multifaceted biomimetic periosteum with a lamellar architecture and osteogenic/angiogenic dual bioactivities (2023-04-11)
期刊:Biomaterials Science
影响因子 :6.6
引用产品: 磷酸盐缓冲液 (PBS) 1× , DMEM高糖完全培养基 (含10%FBS)
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Anti-inflammatory effects of 6S-5-methyltetrahydrofolate‐calcium on RAW264.7 cells and zebrafish (2023-06-07)
期刊:LIFE SCIENCES
影响因子 :6.1
引用产品: RAW 264.7 细胞 , DMEM高糖完全培养基 (含10%FBS)
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Antibacterial MccM as the Major Microcin in Escherichia coli Nissle 1917 against Pathogenic Enterobacteria (2023-07-20)
期刊:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
影响因子 :5.6
引用产品: DMEM高糖完全培养基 (含10%FBS)
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Aucubin improves chronic unpredictable mild stress-induced depressive behavior in mice via the GR/NF-κB/NLRP3 axis (2023-07-29)
期刊:INTERNATIONAL IMMUNOPHARMACOLOGY
DOI:10.1016/j.intimp.2023.110677
影响因子 :5.6
引用产品: DMEM高糖完全培养基 (含10%FBS)
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Circ_0001861 facilitates trophoblast cell proliferation, migration, invasion and epithelial-mesenchymal transition via the miR-296-5p/forkhead box protein 1 pathway in preeclampsia (2023-12-29)
期刊:JOURNAL OF HYPERTENSION
DOI:10.1097/HJH.0000000000003634
影响因子 :4.9
引用产品: DMEM高糖完全培养基 (含10%FBS)
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Circ_0001806 relieves LPS-induced HK2 cell injury by regulating the expression of miR-942-5p and TXNIP (2023-08-05)
期刊:JOURNAL OF BIOENERGETICS AND BIOMEMBRANES
DOI:10.1007/s10863-023-09978-3
影响因子 :3.0
引用产品: DMEM高糖完全培养基 (含10%FBS)
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A New Monoterpene Alkaloid from Incarvillea sinensis with Migration Inhibitory Activity on Cancer Cell (2023-03-09)
期刊:CHEMISTRY & BIODIVERSITY
影响因子 :2.9
引用产品: DMEM高糖 培养基 , 特级胎牛血清 , MDA-MB-231 细胞 , DMEM高糖完全培养基 (含10%FBS)
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Regulatory mechanism of DHRS2-modified human umbilical cord mesenchymal stem cells-derived exosomes in prostate cancer cell proliferation and apoptosis (2023-03-24)
期刊:TISSUE & CELL
DOI:10.1016/j.tice.2023.102078
影响因子 :2.6
引用产品: DMEM高糖完全培养基 (含10%FBS) , 人脐带间充质干细胞成骨诱导分化培养基 , 人脐带间充质干细胞成脂诱导分化培养基
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Inhibition of VDAC1 prevents oxidative stress and apoptosis induced by bisphenol A in spermatogonia via AMPK/mTOR signaling pathway (2023-03-01)
期刊:JOURNAL OF TOXICOLOGICAL SCIENCES
影响因子 :2.0
引用产品: DMEM高糖完全培养基 (含10%FBS)
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Biomechanically-Adapted Immunohydrogels Reconstructing Myelin Sheath for Peripheral Nerve Regeneration (2022-08-03)
期刊:Advanced Healthcare Materials
影响因子 :11.1
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ITGA2 overexpression inhibits DNA repair and confers sensitivity to radiotherapies in pancreatic cancer (2022-08-20)
期刊:CANCER LETTERS
DOI:10.1016/j.canlet.2022.215855
影响因子 :9.8
引用产品: DMEM高糖完全培养基 (含10%FBS)
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Osteoblast Jmjd3 regulates osteoclastogenesis via EphB4 and RANKL signalling (2002-02-20)
期刊:Oral Diseases
影响因子 :3.8
引用产品: RAW 264.7 细胞 , DMEM高糖完全培养基 (含10%FBS)
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LncRNA MALAT1 aggravates the retinal angiogenesis via miR-320a/HIF-1α axis in diabetic retinopathy (2002-02-20)
期刊:Experimental Eye Research
DOI:10.1016/j.exer.2022.108984
影响因子 :3.4
引用产品: 小鼠视网膜Muller细胞 , 小鼠视网膜微血管内皮细胞 , 293T [HEK-293T] 细胞 , DMEM/F12完全培养基 (含10%FBS) , DMEM高糖完全培养基 (含10%FBS)
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Artesunate restrains the malignant progression of human cutaneous squamous cell carcinoma cells via the suppression of the PI3K/AKT pathway (2003-02-20)
期刊:Tissue & Cell
DOI:10.1016/j.tice.2022.101789
影响因子 :2.6
引用产品: A-431 细胞 , DMEM高糖完全培养基 (含10%FBS)
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Circ_0044516 Enriches the Level of SARM1 as a miR-330-5p Sponge to Regulate Cell Malignant Behaviors and Tumorigenesis of Prostate Cancer (2001-02-20)
期刊:Biochemical Genetics
DOI:10.1007/s10528-021-10160-w
影响因子 :2.4
引用产品: DU 145 细胞 , LNCaP clone FGC 细胞 , DMEM高糖完全培养基 (含10%FBS) , RPMI-1640完全培养基 (含10%FBS) , RWPE-1 细胞
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Ten-gene signature reveals the significance of clinical prognosis and immuno-correlation of osteosarcoma and study on novel skeleton inhibitors regarding MMP9 (2007-01-20)
期刊:Cancer Cell International
DOI:10.1186/s12935-021-02041-4
影响因子 :5.8
引用产品: HOS 细胞 , MG-63 细胞 , DMEM高糖完全培养基 (含10%FBS)
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Bergenin Monohydrate Attenuates Inflammatory Response via MAPK and NF-κB Pathways Against Klebsiella pneumonia Infection (2005-01-20)
期刊:Frontiers in Pharmacology
影响因子 :5.6
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Exosomal transfer of activated neutrophil-derived lncRNA CRNDE promotes proliferation and migration of airway smooth muscle cells in asthma (2009-01-20)
期刊:Human Molecular Genetics
影响因子 :3.5
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miR-339-5p Inhibits Autophagy to Reduce the Resistance of Laryngeal Carcinoma on Cisplatin via Targeting TAK1 (2008-01-20)
期刊:Biomed Research International
影响因子 :3.2
引用产品: DMEM高糖完全培养基 (含10%FBS)
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In vitro facilitating role of polygonatum sibiricum polysaccharide in osteogenic differentiation of bone marrow mesenchymal stem cells from patients with multiple myeloma (2004-01-20)
期刊:Biotechnology Letters
DOI:10.1007/s10529-021-03125-x
影响因子 :2.7
引用产品: DMEM高糖完全培养基 (含10%FBS)
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Systematic understanding of the potential targets and pharmacological mechanisms of acteoside by network pharmacology approach (2002-00-20)
期刊:Medicinal Chemistry Research
DOI:10.1007/s00044-020-02524-5
影响因子 :2.6
引用产品: Hep G2 细胞 , DMEM高糖完全培养基 (含10%FBS)
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A novel pH-sensitive targeting polysaccharide-gold nanorod conjugate for combined photothermal-chemotherapy of breast cancer (2002-09-20)
期刊:Carbohydrate Polymers
DOI:10.1016/j.carbpol.2019.02.045
影响因子 :11.2
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Doxorubicin/cisplatin co-loaded hyaluronic acid/chitosan-based nanoparticles for in vitro synergistic combination chemotherapy of breast cancer (2008-09-20)
期刊:Carbohydrate Polymers
DOI:10.1016/j.carbpol.2019.115206
影响因子 :11.2
引用产品: DMEM高糖完全培养基 (含10%FBS)
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PEGylated hydrazided gold nanorods for pH-triggered chemo/photodynamic/photothermal triple therapy of breast cancer (2010-08-20)
期刊:Acta Biomaterialia
DOI:10.1016/j.actbio.2018.10.019
影响因子 :9.3
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A dual-targeted hyaluronic acid-gold nanorod platform with triple-stimuli responsiveness for photodynamic/photothermal therapy of breast cancer (2011-08-20)
期刊:Acta Biomaterialia
DOI:10.1016/j.actbio.2018.11.026
影响因子 :9.3
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A double-network poly(Nɛ-acryloyl l-lysine)/hyaluronic acid hydrogel as a mimic of the breast tumor microenvironment (2001-06-20)
期刊:Acta Biomaterialia
DOI:10.1016/j.actbio.2016.01.027
影响因子 :9.3
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Preparation, characterization, and biocompatibility evaluation of poly(Nɛ-acryloyl-l-lysine)/hyaluronic acid interpenetrating network hydrogels (2009-05-20)
期刊:Carbohydrate Polymers
DOI:10.1016/j.carbpol.2015.09.095
影响因子 :11.2
引用产品: DMEM高糖完全培养基 (含10%FBS)
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