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产品概述
产品信息
| 别名 | Fibroblast Growth Factor 2; FGF-2; Basic fibroblast Growth Factor; Bfgf; Heparin-binding Growth Factor 2; HBGF-2; FGF2; FGFB |
| 物种 | Human |
| 表达宿主 | E.coli |
| 序列信息 | Met134-Ser288 |
| 检索号 | P09038 |
| 分子量 | 17.3 kDa |
| 表观分子量 | 17 kDa |
| 生物活性 | Measured in a cell proliferation assay using Balb/3T3 mouse fibroblast cells. The ED50 for this effect is 0.1-0.6 ng/ml. |
产品特性
| 纯度 | >95% as determined by reducing SDS-PAGE. |
| 内毒素 | <1.0 EU/µg as determined by LAL test. |
| 保存 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
| 运输 | Ambient temperature or ice pack. |
| 制剂 | Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 8% Trehalose, 2% Mannitol, 0.05% Tween80, 2mM EDTA, pH8.0. |
| 复融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
背景介绍
Fibroblast Growth Factor 2 (FGF2) is a secreted Protein and belongs to the heparin-binding Growth Factors family. FGF2 is produced by epithelial, tumor and other cell types. It involved in developmental processes and regulates differentiation, proliferation, and migration, FGF2 is a critical factor for growing embryonic stem cells in culture without inducing differentiation. FGF2 has a high affinity for heparan sulfate and binding is a step in the FGF basic activation of FGFR tyrosine kinase.
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