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产品概述
产品信息
| 别名 | Urogastrone, URG |
| 物种 | Human |
| 表达宿主 | E.coli |
| 序列信息 | MNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELR with polyhistidine tag at the C-terminus |
| 检索号 | P01133.2 |
| 分子量 | 7.16 kDa |
| 表观分子量 | 9-11 kDa |
| 标签 | His-tag at the C-terminus |
| 生物活性 | Measure by its ability to induce 3T3 cells proliferation. The ED50 for this effect is 0.05-0.12 ng/mL. The specific activity of recombinant human EGF is approximately >1.4 x 10^6 IU/mg. |
产品特性
| 纯度 | >95% as determined by SDS-PAGE. Ni-NTA chromatography. |
| 内毒素 | <0.1 EU per 1 μg of the protein by the LAL method. |
| 保存 | Lyophilized protein should be stored at -5~-20°C for 1 year. Upon reconstitution, store at 2-8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g.0.1% BSA,10%FBS,5%HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20°C or -80°C for 3-6 months. |
| 运输 | Ambient temperature or ice pack. |
| 制剂 | The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0. |
| 复融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL.Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
背景介绍
参考文献
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Acidosis activates breast cancer ferroptosis through ZFAND5/SLC3A2 signaling axis and elicits M1 macrophage polarization (2024-02-14)
作者：Hanchu Xiong:1.Cancer Center, Department of Radiation Oncology, Zhejiang Provincial People's Hospital (Affiliated People's Hospital), Hangzhou Medical College, Hangzhou, Zhejiang, China. ; Yanan Zhai:1.Cancer Center, Department of Medical Oncology, Zhejiang Provincial People's Hospital (Affiliated People's Hospital), Hangzhou Medical College, Hangzhou, Zhejiang, China. ; Yimei Meng:1.Cancer Center, Department of Medical Oncology, Zhejiang Provincial People's Hospital (Affiliated People's Hospital), Hangzhou Medical College, Hangzhou, Zhejiang, China. ; Zhuazhua Wu:1.Cancer Center, Department of Medical Oncology, Zhejiang Provincial People's Hospital (Affiliated People's Hospital), Hangzhou Medical College, Hangzhou, Zhejiang, China. ; Anchen Qiu:1.Cancer Center, Department of Medical Oncology, Zhejiang Provincial People's Hospital (Affiliated People's Hospital), Hangzhou Medical College, Hangzhou, Zhejiang, China. ; Yu Cai:1.Cancer Center, Department of Medical Oncology, Zhejiang
期刊：Acidosis activates breast cancer ferroptosis through ZFAND5/SLC3A2 signaling axis and elicits M1 macrophage polarization
DOI：10.1016/j.canlet.2024.216732
影响因子 ：9.7
引用产品： McCoy's 5A 培养基 , DMEM高糖 培养基 , 马血清 , EGF/Urogastrone/URG, Human, Recombinant , 重组人胰岛素溶液 (10mg/mL) , MEM非必需氨基酸溶液 (NEAA)，100× , 青霉素-链霉素溶液 (双抗)，100× , RPMI-1640 培养基
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Characterization of Cancer Cell Mechanics by Measuring Active Deformation Behavior (2023-09-29)
作者：Jiantao Feng:1.Artemisinin Research Center and Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China. ; Quanmei Sun:1.CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing, 100190, China. ; Peipei Chen:1.CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing, 100190, China. ; Keli Ren:1.The Institute of Physics, Chinese Academy of Sciences, Beijing, 100190, China. ; Yuanyuan Zhang:1.Cancer Hospital, Chinese Academy of Medical Sciences, Beijing, 100021, China. ; Yahong Shi:1.CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing, 100190, China. ; Songkun Gao:1.Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, 100006, China. ; Zhiwei Song:1.CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing, 100190, China. ; Jigang Wang:1.Artem
期刊：Characterization of Cancer Cell Mechanics by Measuring Active Deformation Behavior
影响因子 ：12.4
引用产品： DMEM/F12 培养基 , 马血清 , EGF/Urogastrone/URG, Human, Recombinant , 重组人胰岛素溶液 (10mg/mL) , MEM非必需氨基酸溶液 (NEAA)，100× , Hela 细胞 , MCF 10A 细胞
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DNA Nanoribbon-Assisted Intracellular Biosynthesis of Fluorescent Gold Nanoclusters for Cancer Cell Imaging (2023-09-06)
作者：Xiangyuan Ouyang:1.Xi'an Key Laboratory of Functional Supramolecular Structure and Materials, Key Laboratory of Synthetic and Natural Functional Molecule of Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi'an, Shaanxi 710127, P. R. China. ; Nan Jia:1.Xi'an Key Laboratory of Functional Supramolecular Structure and Materials, Key Laboratory of Synthetic and Natural Functional Molecule of Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi'an, Shaanxi 710127, P. R. China. ; Jing Luo:1.Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), College of Life Sciences, Northwest University, Xi'an, Shaanxi 710069, PR China. ; Le Li:1.Xi'an Key Laboratory of Functional Supramolecular Structure and Materials, Key Laboratory of Synthetic and Natural Functional Molecule of Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi'an, Shaanxi 71012
期刊：DNA Nanoribbon-Assisted Intracellular Biosynthesis of Fluorescent Gold Nanoclusters for Cancer Cell Imaging
影响因子 ：8.0
引用产品： DMEM/F12 培养基 , 重组人胰岛素溶液 (10mg/mL) , MEM非必需氨基酸溶液 (NEAA)，100× , EGF/Urogastrone/URG, Human, Recombinant , 马血清 , MTT溶液 (5mg/mL) , MCF 10A 细胞
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Z-Guggulsterone Induces Cell Cycle Arrest and Apoptosis by Targeting the p53/CCNB1/PLK1 Pathway in Triple-Negative Breast Cancer (2023-01-03)
作者：Yihao Wu:1.College of Pharmacy, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, China. ; Tao Zhou:1.Zhejiang Provincial People's Hospital, Affiliated People's Hospital, Hangzhou Medical College, Hangzhou 310053, Zhejiang, China. ; Da Qian:1.Department of Burn and Plastic Surgery-Hand Surgery, Changshu Hospital Affiliated to Soochow University, Changshu No. 1 People's Hospital, Changshu 215500, Jiangsu, China. ; Xiaozhen Liu:1.General Surgery, Cancer Center, Department of Breast Surgery, Zhejiang Provincial People's Hospital (Affiliated People's Hospital, Hangzhou Medical College), Hangzhou 310053, Zhejiang, China. ; Yuhao Xu:1.The Second Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang, China. ; Weimin Hong:1.Zhejiang Provincial People's Hospital, Affiliated People's Hospital, Hangzhou Medical College, Hangzhou 310053, Zhejiang, China. ; Xuli Meng:1.General Surgery, Cancer Center, Department of Breast Surgery, Zhejiang Provinc
期刊：Z-Guggulsterone Induces Cell Cycle Arrest and Apoptosis by Targeting the p53/CCNB1/PLK1 Pathway in Triple-Negative Breast Cancer
影响因子 ：4.1
引用产品： DMEM/F12 培养基 , 马血清 , EGF/Urogastrone/URG, Human, Recombinant , 重组人胰岛素溶液 (10mg/mL) , MEM非必需氨基酸溶液 (NEAA)，100×
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Circ_0005276 Promotes Prostate Cancer Progression Through the Crosstalk of miR-128-3p/DEPDC1B Axis (2023-03-13)
作者：Wenwei Li:1.Department of Urology WardIII, Huangshi Central Hospital, Affiliated Hospital of Hubei Polytechnic University, Edong Healthcare Group, No.293, Feiyun Street, Huangshi, 435000, Hubei Province, China. ; 2.Hubei Key Laboratory of Kidney Disease Pathogenesis and Intervention, Wuhan, China. ; Wenbing Wu:1.Department of Urology WardIII, Huangshi Central Hospital, Affiliated Hospital of Hubei Polytechnic University, Edong Healthcare Group, No.293, Feiyun Street, Huangshi, 435000, Hubei Province, China. hszxyy1193868501@163.com. ; 2.Hubei Key Laboratory of Kidney Disease Pathogenesis and Intervention, Wuhan, China. hszxyy1193868501@163.com. ;
期刊：Circ_0005276 Promotes Prostate Cancer Progression Through the Crosstalk of miR-128-3p/DEPDC1B Axis
DOI：10.1007/s10528-022-10328-y
影响因子 ：2.4
引用产品： LNCaP clone FGC 细胞 , DU 145 细胞 , RPMI-1640 培养基 , 特级胎牛血清 , RWPE-1 细胞 , K-SFM 培养基 , EGF/Urogastrone/URG, Human, Recombinant
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Activating SIRT3 in peritoneal mesothelial cells alleviates postsurgical peritoneal adhesion formation by decreasing oxidative stress and inhibiting the NLRP3 inflammasome (2022-09-13)
作者：Tianli Shen:1.Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, 710061, Xi'an, Shaanxi, China. ; Yunhua Wu:1.Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, 710061, Xi'an, Shaanxi, China. ; 2.Department of General Surgery, Shaanxi Provincial People's Hospital, 710061, Xi'an, Shaanxi, China. ; Xingjie Wang:1.Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, 710061, Xi'an, Shaanxi, China. ; Zijun Wang:1.Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, 710061, Xi'an, Shaanxi, China. ; Enmeng Li:1.Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, 710061, Xi'an, Shaanxi, China. ; Cancan Zhou:1.Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, 710061, Xi'an, Shaanxi, China. ; Chenyang Yue:1.Department of Biology, York University, Toron
期刊：Activating SIRT3 in peritoneal mesothelial cells alleviates postsurgical peritoneal adhesion formation by decreasing oxidative stress and inhibiting the NLRP3 inflammasome
DOI：10.1038/s12276-022-00848-3
影响因子 ：12.2
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Tumor-suppressive E3 ubiquitin ligase CHIP inhibits the PBK/ERK axis to repress stem cell properties and radioresistance in non-small cell lung cancer (2022-11-27)
作者：Bo Tan:1.Department of Radiotherapy, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, 127 Dongming Road, Jinshui, Zhengzhou, 450008, Henan, China. zlyytanbo3069@zzu.edu.cn. ; Jingwei Zhang:1.Department of Radiotherapy, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, 127 Dongming Road, Jinshui, Zhengzhou, 450008, Henan, China. ; Wen Wang:1.Department of Radiotherapy, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, 127 Dongming Road, Jinshui, Zhengzhou, 450008, Henan, China. ; Haibo Ma:1.Department of Thoracic Surgery, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, Zhengzhou, 450008, China. ; Yuanyuan Yang:1.Department of Radiotherapy, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, 127 Dongming Road, Jinshui, Zhengzhou, 450008, Henan, China. ;
期刊：Tumor-suppressive E3 ubiquitin ligase CHIP inhibits the PBK/ERK axis to repress stem cell properties and radioresistance in non-small cell lung cancer
DOI：10.1007/s10495-022-01789-y
影响因子 ：5.6
引用产品： PC-9 细胞 , A549 [A-549] 细胞 , NCI-H1299 细胞 , NCI-H1975 细胞 , BEAS-2B 细胞 , RPMI-1640 培养基 , BEAS-2B细胞专用培养基 , EGF/Urogastrone/URG, Human, Recombinant , FGF-2/ bFGF/ FGF-b (Q65I, C96S, N111G), Human, Recombinant
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