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产品概述
产品信息
| 别名 | HBGF-2, Prostatropin |
| 物种 | Human |
| 表达宿主 | E.coli |
| 序列信息 | AAGSITTLPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS with polyhistidine tag at the N-terminus. |
| 检索号 | P09038.3 |
| 分子量 | 18.1 kDa |
| 表观分子量 | 17 kDa |
| 标签 | His-tag at the N-terminus |
| 生物活性 | Measure by its ability to induce 3T3 cells proliferation.The ED50 for this effect is <1 ng/mL.The specific activity of recombinant human FGF-2 is approximately >5 x 10^5 IU/mg. |
产品特性
| 纯度 | >98% as determined by SDS-PAGE. Ni-NTA chromatography |
| 内毒素 | <0.1 EU per 1 μg of the protein by the LAL method. |
| 保存 | Lyophilized protein should be stored at -5~-20°C for 1 year. Upon reconstitution, store at 2-8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g.0.1% BSA,10%FBS,5%HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20°C or -80°C for 3-6 months. |
| 运输 | Ambient temperature or ice pack. |
| 制剂 | The protein was lyophilized from a 0.2 µm filtered solution containing 0.01% sarkosyl in 1X PBS, pH 8.0. |
| 复融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL.Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
背景介绍
参考文献
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Tumor-suppressive E3 ubiquitin ligase CHIP inhibits the PBK/ERK axis to repress stem cell properties and radioresistance in non-small cell lung cancer (2022-11-27)
作者：Bo Tan:1.Department of Radiotherapy, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, 127 Dongming Road, Jinshui, Zhengzhou, 450008, Henan, China. zlyytanbo3069@zzu.edu.cn. ; Jingwei Zhang:1.Department of Radiotherapy, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, 127 Dongming Road, Jinshui, Zhengzhou, 450008, Henan, China. ; Wen Wang:1.Department of Radiotherapy, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, 127 Dongming Road, Jinshui, Zhengzhou, 450008, Henan, China. ; Haibo Ma:1.Department of Thoracic Surgery, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, Zhengzhou, 450008, China. ; Yuanyuan Yang:1.Department of Radiotherapy, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, 127 Dongming Road, Jinshui, Zhengzhou, 450008, Henan, China. ;
期刊：Tumor-suppressive E3 ubiquitin ligase CHIP inhibits the PBK/ERK axis to repress stem cell properties and radioresistance in non-small cell lung cancer
DOI：10.1007/s10495-022-01789-y
影响因子 ：5.6
引用产品： PC-9 细胞 , A549 [A-549] 细胞 , NCI-H1299 细胞 , NCI-H1975 细胞 , BEAS-2B 细胞 , RPMI-1640 培养基 , BEAS-2B细胞专用培养基 , EGF/Urogastrone/URG, Human, Recombinant , FGF-2/ bFGF/ FGF-b (Q65I, C96S, N111G), Human, Recombinant
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