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产品概述
EDTA（乙二胺四乙酸）溶液是一种化学螯合剂，能够螯合Ca2+、Mg2+等二价金属离子，其溶液又称Versene液。由于Ca2+、Mg2+是细胞膜的重要组成成分，具有使细胞凝集的作用，因此EDTA可以破坏细胞间的连接，促使细胞离解成单个细胞。EDTA毒性少、价格便宜，使用方便，且不会破坏细胞表面分子，仅与Ca2+、Mg2+结合，所以适用于需检测细胞表面分子的细胞消化，也常与胰蛋白酶溶液按一定比例混合使用。
产品信息
形态 | 液体 |
规格 | 100mL |
PH | 7.2-7.4 |
溶剂 | 10 mM PBS |
EDTA•2Na | 0.2 g/L |
酚红指示剂 | 无 |
储存条件 | 2-8℃ |
运输条件 | 常温 |
有效期 | 18个月 |
注意事项
1、EDTA不会被中和，消化下来的细胞要用PBS洗涤1遍，否则易造成细胞贴壁困难；
2、使用本产品时应注意无菌操作，避免污染。
参考文献
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Cancer Cell Membrane-Coated Nanoparticle Co-loaded with Photosensitizer and Toll-like Receptor 7 Agonist for the Enhancement of Combined Tumor Immunotherapy (2023-08-22)
作者：Yanlin Chen:1.Guangdong Provincial Key Laboratory of New Drug Screening and NMPA Key Laboratory for Research and Evaluation of Drug Metabolism, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, People's Republic of China. ; Siying Zhi:1.Guangdong Provincial Key Laboratory of New Drug Screening and NMPA Key Laboratory for Research and Evaluation of Drug Metabolism, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, People's Republic of China. ; Jiaxin Ou:1.Guangdong Provincial Key Laboratory of New Drug Screening and NMPA Key Laboratory for Research and Evaluation of Drug Metabolism, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, People's Republic of China. ; Junbin Gao:1.Guangdong Provincial Key Laboratory of New Drug Screening and NMPA Key Laboratory for Research and Evaluation of Drug Metabolism, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, People'
期刊：Cancer Cell Membrane-Coated Nanoparticle Co-loaded with Photosensitizer and Toll-like Receptor 7 Agonist for the Enhancement of Combined Tumor Immunotherapy
影响因子 ：17.1
引用产品： 0.02% EDTA溶液 (Versene液)
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MicroRNA-195 liposomes for therapy of Alzheimer's disease (2023-12-06)
作者：Dan Su:1.Department of Pharmacology (The State-Province Key Laboratories of Biomedicine-Pharmaceutics of China), College of Pharmacy of Harbin Medical University, Harbin, Heilongjiang Province, China. ; Zhong Chen:1.National Key Laboratory of Frigid Zone Cardiovascular Diseases, China. ; Xiaobin An:1.Department of Pharmacology (The State-Province Key Laboratories of Biomedicine-Pharmaceutics of China), College of Pharmacy of Harbin Medical University, Harbin, Heilongjiang Province, China. ; Junkai Yang:1.Department of Pharmacology (The State-Province Key Laboratories of Biomedicine-Pharmaceutics of China), College of Pharmacy of Harbin Medical University, Harbin, Heilongjiang Province, China. ; Jinan Yang:1.Department of Pharmacology (The State-Province Key Laboratories of Biomedicine-Pharmaceutics of China), College of Pharmacy of Harbin Medical University, Harbin, Heilongjiang Province, China. ; Xuqiao Wang:1.Department of Pharmacology (The State-Province Key Laboratories of Bio
期刊：MicroRNA-195 liposomes for therapy of Alzheimer's disease
DOI：10.1016/j.jconrel.2023.12.003
影响因子 ：10.8
引用产品： 0.02% EDTA溶液 (Versene液)
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Insights into the characteristics of primary radioresistant cervical cancer using single-cell transcriptomics (2023-03-03)
作者：Biyuan Xing:1.Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. ; 2.Institute of Radiation Oncology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. ; Congli Pu:1.Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. ; 2.Institute of Radiation Oncology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. ; Yunshang Chen:1.Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. ; 2.Institute of Radiation Oncology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. ; Yuhan Sheng:1.Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. ;
期刊：Insights into the characteristics of primary radioresistant cervical cancer using single-cell transcriptomics
DOI：10.1007/s13577-023-00882-x
影响因子 ：4.3
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Runx3 expression in rectal cancer cells and its effect on cell invasion and proliferation (2007-09-20)
作者：Ye Feng:1.Department of Gastrointestinal Colorectal and Anal Surgery, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China. ; Shuohui Gao:1.Department of Gastrointestinal Colorectal and Anal Surgery, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China. ; Yongjian Gao:1.Department of Gastrointestinal Colorectal and Anal Surgery, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China. ; Defeng Song:1.Department of Gastrointestinal Colorectal and Anal Surgery, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China. ; Xuefeng Wang:1.Department of Gastrointestinal Colorectal and Anal Surgery, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China. ; Zhi Chen:1.Department of Nephropathy, The First Bethune Hospital of Jilin University, Changchun, Jilin 130021, P.R. China. ;
期刊：Runx3 expression in rectal cancer cells and its effect on cell invasion and proliferation
影响因子 ：2.9
引用产品： RPMI-1640 培养基 , 0.02% EDTA溶液 (Versene液)
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