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产品概述
胰蛋白酶(Trypsin)是一种丝氨酸水解酶,它能把多肽链中赖氨酸和精氨酸残基中的羧基侧切段,水解细胞间的蛋白质,破坏细胞间的连接,从而使组织或贴壁细胞离散成单个细胞。胰酶分散细胞的活性与组织或细胞的特性、胰酶浓度、温度和作用时间有关,在PH 8.0和37℃时,胰酶的作用能力最强,因此使用胰酶时,应把握好浓度、温度和时间,以免消化过度造成细胞损伤。
一般常用胰酶的工作浓度为0.25%,而半贴壁细胞或对胰酶敏感的细胞常采用低浓度(0.05%)的胰酶进行细胞消化。由于EDTA能够螯合Ca2+和Mg2+,从而破坏细胞连接促进细胞的解离,因此在胰酶溶液中常常会加入一定量的EDTA混合使用,以增强解离效果。
产品信息
形态 | 液体 |
规格 | 100mL |
PH | 7.2-7.4 |
溶剂 | 10mM PBS |
胰蛋白酶浓度 | 2.5g/L |
EDTA•2Na | 无 |
酚红指示剂 | 无 |
储存条件 | -5~-20℃ |
运输条件 | 冷冻 |
有效期 | 12个月 |
注意事项
1、由于不同的组织或者细胞对胰酶的作用反应不一样,因此操作人员应根据实际情况,确定最佳消化时间;消化细胞时间不宜过长,否则会影响细胞贴壁和生长状况;
2、使用本产品时应注意无菌操作,避免污染;
3、不宜长时间放置于室温环境或2~8℃长期保存;
4、2~8℃中解冻,摇匀后使用,切忌反复冻融,用量较少时建议分装冻存。
5、本产品仅用于科研或进一步研究使用,不用于诊断和治疗。
参考文献
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Thalidomide suppresses migration and invasion of colorectal cancer cells by inhibiting HOXB7-mediated activation of the Wnt/β-catenin signaling pathway (2024-01-05)
作者:Liyang Liu:1.Department of Anoretal, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, China. ; Wusong Xue:1.Department of Anoretal, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, China. ;
期刊:Thalidomide suppresses migration and invasion of colorectal cancer cells by inhibiting HOXB7-mediated activation of the Wnt/β-catenin signaling pathway
影响因子 :3.0
引用产品: HCT-8 [HRT-18] 细胞 , SW480 [SW-480]细胞专用培养基 (L15) , Caco-2细胞专用培养基 , RPMI-1640 培养基 , 青霉素-链霉素溶液 (双抗),100× , 0.25%胰蛋白酶溶液 (溶于PBS)
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Combined toxicity of oil-based PAH4 mixtures on HL-7702 cells (2023-12-05)
作者:Xiaofang Liu:1.School of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China; School of Tourism and Cuisine, Yangzhou University, Yangzhou 225127, China. ; Xu Zhang:1.School of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China. ; Wanli Zhou:1.School of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China. ; Li Liang:1.School of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China. ; Jixian Zhang:1.School of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China. ; Chaoting Wen:1.School of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China. ; Youdong Li:1.School of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China. ; Xin Xu:1.School of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China. ; Guoyan Liu:1.School of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China. Electronic address:
期刊:Combined toxicity of oil-based PAH4 mixtures on HL-7702 cells
DOI:10.1016/j.scitotenv.2023.169038
影响因子 :9.8
引用产品: RPMI-1640 培养基 , 青霉素-链霉素溶液 (双抗),100× , 特级胎牛血清 , 0.25%胰蛋白酶溶液 (溶于PBS) , 磷酸盐缓冲液 (PBS) 1× , HL-7702 [L-02; LO2] 细胞
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Near-infrared light-mediated CuS@Rutin nanocomposites for the PTT/PDT synergistic treatment in bacterial infections (2023-12-06)
作者:Jian Liu: 1.Department of Stomatology, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao 266003, China;2.School of Stomatology, Qingdao University, Qingdao 266003, China; Baoheng Yin: 1.Department of Stomatology, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao 266003, China;2.School of Stomatology, Qingdao University, Qingdao 266003, China; Shuang Li: 1.Department of Stomatology, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao 266003, China;2.School of Stomatology, Qingdao University, Qingdao 266003, China; Lian Mi: 1.Department of Stomatology, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao 266003, China;2.School of Stomatology, Qingdao University, Qingdao 266003, China; Guanghao Yin: 1.Institute of Stomatology, Binzhou Medical University, Yantai 264003, China; Feng Li: 1.School of Stomatology, Qingdao University, Qingdao 266003, China; Lingmei Zhong: 1.Department of Pulmonary and
期刊:Near-infrared light-mediated CuS@Rutin nanocomposites for the PTT/PDT synergistic treatment in bacterial infections
DOI:10.1016/j.jiec.2023.12.006
影响因子 :6.1
引用产品: 0.25%胰蛋白酶溶液 (溶于PBS) , DMEM高糖 培养基 , 特级胎牛血清
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Searching for novel MDM2/MDMX dual inhibitors through a drug repurposing approach (2023-12-07)
作者:Keting Li:1.School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China. ; Wenshu Hu:1.School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China. ; Yingjie Wang:1.School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China. ; Wenxing Chen:1.School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China. ; Hongmei Wen:1.School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China. ; Jian Liu:1.School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China. ; Wei Li:1.School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China. ; Bo Wang:1.School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China. ;
期刊:Searching for novel MDM2/MDMX dual inhibitors through a drug repurposing approach
DOI:10.1080/14756366.2023.2288810
影响因子 :5.6
引用产品: DMEM高糖 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗),100× , 0.25%胰蛋白酶溶液 (溶于PBS)
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Analysis of the mechanism of curcumin against osteoarthritis using metabolomics and transcriptomics (2023-11-08)
作者:Wenxiang Deng:1.College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, 410208, Hunan, China. ; Qinghu He:1.Department of Rehabilitation and Healthcare, Hunan University of Medicine, Huaihua, 418000, Hunan, China. hqh19651111@dingtalk.com. ; 2.College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, 410208, Hunan, China. hqh19651111@dingtalk.com. ; Wenan Zhang:1.College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, 410208, Hunan, China. ;
期刊:Analysis of the mechanism of curcumin against osteoarthritis using metabolomics and transcriptomics
DOI:10.1007/s00210-023-02785-y
影响因子 :3.6
引用产品: 人关节软骨细胞 , 人关节软骨细胞完全培养基 , 0.25%胰蛋白酶溶液 (溶于PBS)
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EZH2 Promotes Corneal Endothelial Cell Apoptosis by Mediating H3K27me3 and Inhibiting HO-1 Transcription (2023-10-05)
作者:Ying Lin:1.Department of Ophthalmology, Liuzhou Workers' Hospital, Liuzhou, Guangxi, China. ; Huanjun Su:1.Department of Ophthalmology, Liuzhou Workers' Hospital, Liuzhou, Guangxi, China. ; Baoyi Zou:1.Department of Optometry, Liuzhou Workers' Hospital, Liuzhou, Guangxi, China. ; Minli Huang:1.Department of Optometry, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China. ;
期刊:EZH2 Promotes Corneal Endothelial Cell Apoptosis by Mediating H3K27me3 and Inhibiting HO-1 Transcription
DOI:10.1080/02713683.2023.2257401
影响因子 :2.0
引用产品: 0.25%胰蛋白酶溶液 (溶于PBS)
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Ubenimex suppresses glycolysis mediated by CD13/Hedgehog signaling to enhance the effect of cisplatin in liver cancer (2023-09-28)
作者:Yunyan Shi:1.Department of Pharmacology, Qingdao University, Qingdao, China. ; Qie Guo:1.Department of Clinical Pharmacy, The Affiliated Hospital of Qingdao University, Qingdao, China. ; Fanjing Jing:1.Department of Lymphoma, The Affiliated Hospital of Qingdao University, Qingdao, China. ; Xiuling Shang:1.Department of Clinical Pharmacy, The Affiliated Hospital of Qingdao University, Qingdao, China. ; Changkai Zhou:1.Department of Clinical Pharmacy, The Affiliated Hospital of Qingdao University, Qingdao, China. ; Fanbo Jing:1.Department of Clinical Pharmacy, The Affiliated Hospital of Qingdao University, Qingdao, China. ;
期刊:Ubenimex suppresses glycolysis mediated by CD13/Hedgehog signaling to enhance the effect of cisplatin in liver cancer
影响因子 :0.9
引用产品: DMEM高糖 培养基 , MEM 培养基 , 特级胎牛血清 , 0.25%胰蛋白酶溶液 (溶于PBS)
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In vitro and in vivo investigation of the therapeutic mechanism of Lycium Chinense and Cuscutae Semen on oligoasthenozoospermia (2003-01-20)
作者:Jisheng Wang:1.First Clinical Medical College, Beijing University of Chinese Medicine, Beijing, China. ; 2.Andrology Department, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China. ; Binghao Bao:1.First Clinical Medical College, Beijing University of Chinese Medicine, Beijing, China. ; 2.Andrology Department, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China. ; Fanchao Meng:1.First Clinical Medical College, Beijing University of Chinese Medicine, Beijing, China. ; 2.Andrology Department, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China. ; Sheng Deng:1.First Clinical Medical College, Beijing University of Chinese Medicine, Beijing, China. ; 2.Andrology Department, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China. ; Junlong Feng:1.First Clinical Medical College, Beijing University of Chinese Medicine, Beijing, China. ; 2.Andrology Department, Dongzhimen Hospital, Beijing Universit
期刊:In vitro and in vivo investigation of the therapeutic mechanism of Lycium Chinense and Cuscutae Semen on oligoasthenozoospermia
影响因子 :2.4
引用产品: DMEM高糖 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗),100× , 0.25%胰蛋白酶溶液 (溶于PBS)
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Photoinduced reactivity and cytotoxicity of a series of zinc(II)–flavonolate derivative complexes (2003-00-20)
作者:Si-Ying An: 1.Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, National Demonstration Center for Experimental Chemistry Education, Northwest University, **’an, 710069, Shaanxi, People’s Republic of China; Yuan-Yuan Su: 1.Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, National Demonstration Center for Experimental Chemistry Education, Northwest University, **’an, 710069, Shaanxi, People’s Republic of China; **n Qi: 1.Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, National Demonstration Center for Experimental Chemistry Education, Northwest University, **’an, 710069, Shaanxi, People’s Republic of China; Rong-Lan Zhang: 1.Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education,
期刊:Photoinduced reactivity and cytotoxicity of a series of zinc(II)–flavonolate derivative complexes
DOI:10.1007/s11243-020-00377-w
影响因子 :1.7
引用产品: 0.25%胰蛋白酶溶液 (溶于PBS)
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