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产品概述
胰蛋白酶(Trypsin)是一种丝氨酸水解酶,它能把多肽链中赖氨酸和精氨酸残基中的羧基侧切段,水解细胞间的蛋白质,破坏细胞间的连接,从而使组织或贴壁细胞离散成单个细胞。胰酶分散细胞的活性与组织或细胞的特性、胰酶浓度、温度和作用时间有关,在pH 8.0和37℃时,胰酶的作用能力最强,因此使用胰酶时,应把握好浓度、温度和时间,以免消化过度造成细胞损伤。
一般常用胰酶的工作浓度为0.25%,而半贴壁细胞或对胰酶敏感的细胞常采用低浓度(0.05%)的胰酶进行细胞消化。由于EDTA能够螯合Ca2+和Mg2+,从而破坏细胞连接促进细胞的解离,因此在胰酶溶液中常常会加入一定量的EDTA混合使用,以增强解离效果。
产品信息
形态 | 液体 |
规格 | 125mL/500mL |
溶剂 | D-Hank's |
胰蛋白酶浓度 | 2.5 g/L |
EDTA•2Na | 0.2 g/L |
酚红指示剂 | 10 mg/L |
储存条件 | -5~-20℃ |
运输条件 | 低温 |
有效期 | 12个月 |
注意事项
1、由于不同的组织或者细胞对胰酶的作用反应不一样,因此操作人员应根据实际情况,确定最佳消化时间;消化细胞时间不宜过长,否则会影响细胞贴壁和生长状况;
2、使用本产品时应注意无菌操作,避免污染;
3、不宜长时间放置于室温环境或2-8℃长期保存;
4、2-8℃中解冻,摇匀后使用,切忌反复冻融,用量较少时建议分装冻存。
5、由于D-Hank's平衡盐溶液NaHCO3含量较低(0.35g/L),因此该型胰蛋白酶溶液不能用于5% CO2的环境,若放入CO2培养箱,溶液将迅速变酸,使用时应注意;
6、本产品中含有酚红且采用干冰运输,因此在运输过程中包装内会有部分二氧化碳累积,这些二氧化碳可能会进入到溶液中导致PH略微下降,从而导致溶液颜色由粉红色变成橙色。该种橙色的溶液依然可以使用或者也可以采用无菌的2 M氢氧化钠溶液对PH进行微调后使用;
7、本产品仅用于科研或进一步研究使用,不用于诊断和治疗。
参考文献
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A comparison study on polysaccharides extracted from banana flower using different methods: Physicochemical characterization, and antioxidant and antihyperglycemic activities (2024-02-27)
作者:Yayuan Tang:1.Agro-food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, 174 East Daxue Road, 530007 Nanning, China; Guangxi Key Laboratory of Fruits and Vegetables Storage-processing Technology, 174 East Daxue Road, 530007 Nanning, China. ; Zhen Wei:1.Agro-food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, 174 East Daxue Road, 530007 Nanning, China. ; Xuemei He:1.Agro-food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, 174 East Daxue Road, 530007 Nanning, China; Guangxi Banana Preservation and Processing Research Center of Engineering Technology, 174 East Daxue Road, 530007 Nanning, China. Electronic address: xuemeihegx@gxaas.net. ; Dongning Ling:1.Agro-food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, 174 East Daxue Road, 530007 Nanning, China; Guangxi Banana Preservation and Processing Research Center of Engineering Technology, 1
期刊:A comparison study on polysaccharides extracted from banana flower using different methods: Physicochemical characterization, and antioxidant and antihyperglycemic activities
DOI:10.1016/j.ijbiomac.2024.130459
影响因子 :8.2
引用产品: 青霉素-链霉素溶液 (双抗),100× , Dulbecco's磷酸盐缓冲液 (DPBS),不含钙、镁、酚红 , 0.25%胰蛋白酶溶液 (含EDTA、酚红,溶于D-Hank's) , 特级胎牛血清
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Mechanical properties and biological activity of 3D printed silicon nitride materials (2024-02-03)
作者:Xiaofeng Zeng: 1.Faculty of Engineering, University Malaysia Sabah, Sabah, 88000, Malaysia;2.Hengyang Kaixin Special Material Technology Co., Ltd, Hengyang, 421200, China; Coswald Stephen Sipaut: 1.Faculty of Engineering, University Malaysia Sabah, Sabah, 88000, Malaysia; Noor Maizura Ismail: 1.Faculty of Engineering, University Malaysia Sabah, Sabah, 88000, Malaysia; Yuandong Liu: 1.School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, China; Yan yan Farm: 1.Faculty of Engineering, University Malaysia Sabah, Sabah, 88000, Malaysia; Jiayu He: 1.School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, China;
期刊:Mechanical properties and biological activity of 3D printed silicon nitride materials
DOI:10.1016/j.ceramint.2024.02.041
影响因子 :5.2
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Design, synthesis and biological evaluation of rhein–piperazine–furanone hybrids as potential anticancer agents (2024-02-13)
作者:Yu He:1.State Key Laboratory of High-efficiency Utilization of Coal and Green Chemical Engineering, Ningxia Research Center for Natural Medicine Engineering and Technology, College of Chemistry and Chemical Engineering, Ningxia University 489 Helanshan West Road Yinchuan 750021 China weimengxue@nxu.edu.cn. ; Si-Si Zhang:1.State Key Laboratory of High-efficiency Utilization of Coal and Green Chemical Engineering, Ningxia Research Center for Natural Medicine Engineering and Technology, College of Chemistry and Chemical Engineering, Ningxia University 489 Helanshan West Road Yinchuan 750021 China weimengxue@nxu.edu.cn. ; Meng-Xue Wei:1.State Key Laboratory of High-efficiency Utilization of Coal and Green Chemical Engineering, Ningxia Research Center for Natural Medicine Engineering and Technology, College of Chemistry and Chemical Engineering, Ningxia University 489 Helanshan West Road Yinchuan 750021 China weimengxue@nxu.edu.cn. ;
期刊:Design, synthesis and biological evaluation of rhein–piperazine–furanone hybrids as potential anticancer agents
影响因子 :4.1
引用产品: A549 [A-549] 细胞 , NCI-H460 [H460] 细胞 , PC-9 细胞 , Calu-1 细胞 , SK-BR-3 [SKBR3] 细胞 , SK-OV-3 细胞 , 786-O [786-0] 细胞 , HuH-7 细胞 , HCT 116 细胞 , Hela 细胞 , WI-38 细胞 , MCF 10A 细胞 , KGN 细胞 , 293T [HEK-293T] 细胞 , DMEM高糖 培养基 , MEM 培养基 , DMEM/F12 培养基 , McCoy's 5A 培养基 , RPMI-1640 培养基 , 0.25%胰蛋白酶溶液 (含EDTA,溶于PBS)
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Road dust exposure and human corneal damage in a plateau high geological background provincial capital city: Spatial distribution, sources, bioaccessibility, and cytotoxicity of dust heavy metals (2023-12-07)
作者:Zi-Yue Yang:1.Institute of Environmental Remediation and Human Health, School of Ecology and Environment, Southwest Forestry University, Kunming 650224, China. ; Hai Liu:1.Affiliated Hospital of Yunnan University, Eye Hospital of Yunnan Province, Kunming 650224, China. ; Jing-Ya Li:1.Institute of Environmental Remediation and Human Health, School of Ecology and Environment, Southwest Forestry University, Kunming 650224, China. ; Ya-Bo Bao:1.Institute of Environmental Remediation and Human Health, School of Ecology and Environment, Southwest Forestry University, Kunming 650224, China. ; Ji Yang:1.Affiliated Hospital of Yunnan University, Eye Hospital of Yunnan Province, Kunming 650224, China. ; Li Li:1.Precious Metal Testing Co. LTD of Yunnan Gold Mining Group, Kunming 650215, China. ; Zi-Yu Zhao:1.Precious Metal Testing Co. LTD of Yunnan Gold Mining Group, Kunming 650215, China. ; Qin-Xiang Zheng:1.The Affiliated Ningbo Eye Hospital of Wenzhou Medical University, Ningbo 315040,
期刊:Road dust exposure and human corneal damage in a plateau high geological background provincial capital city: Spatial distribution, sources, bioaccessibility, and cytotoxicity of dust heavy metals
DOI:10.1016/j.scitotenv.2023.169140
影响因子 :9.8
引用产品: 磷酸盐缓冲液 (PBS) 1× , DMEM/F12 培养基 , 特级胎牛血清 , 0.25%胰蛋白酶溶液 (含EDTA,溶于PBS)
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The effect of GelMA/alginate interpenetrating polymeric network hydrogel on the performance of porous zirconia matrix for bone regeneration applications (2023-05-12)
作者:Meiqi Jin:1.School of Intelligent Medicine, China Medical University, Shenyang 110122, China. ; Ningning Sun:1.Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, China Medical University, Shenyang 110002, China. Electronic address: 20072053@cmu.edu.cn. ; Wenxian Weng:1.School of Intelligent Medicine, China Medical University, Shenyang 110122, China. ; Zhentao Sang:1.School of Intelligent Medicine, China Medical University, Shenyang 110122, China. ; Taotao Liu:1.School of Intelligent Medicine, China Medical University, Shenyang 110122, China. ; Wei Xia:1.School of Intelligent Medicine, China Medical University, Shenyang 110122, China. ; Shuze Wang:1.Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, China Medical University, Shenyang 110002, China. ; Xiaoting Sun:1.School of Forensic Medicine, China Medical University, Shenyang 110122, China. Electronic address: xtsun@cmu.edu.cn. ; Tianlin Wang:1.School of Intelligent
期刊:The effect of GelMA/alginate interpenetrating polymeric network hydrogel on the performance of porous zirconia matrix for bone regeneration applications
DOI:10.1016/j.ijbiomac.2023.124820
影响因子 :8.2
引用产品: MEMα 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗),100× , 0.25%胰蛋白酶溶液 (含EDTA,溶于PBS) , MC3T3-E1 Subclone 14 细胞
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CD73 mediates the therapeutic effects of endometrial regenerative cells in concanavalin A-induced hepatitis by regulating CD4+ T cells (2023-09-29)
作者:Hong Qin:1.Department of General Surgery, Tianjin Medical University General Hospital, 154 Anshan Road, Heping District, Tianjin, 300052, China. ; 2.Tianjin General Surgery Institute, Tianjin Medical University General Hospital, Tianjin, 300052, China. ; Chenglu Sun:1.Department of General Surgery, Tianjin Medical University General Hospital, 154 Anshan Road, Heping District, Tianjin, 300052, China. ; 2.Tianjin General Surgery Institute, Tianjin Medical University General Hospital, Tianjin, 300052, China. ; Dejun Kong:1.School of Medicine, Nankai University, Tianjin, 300071, China. ; Yanglin Zhu:1.Department of General Surgery, Tianjin Medical University General Hospital, 154 Anshan Road, Heping District, Tianjin, 300052, China. ; 2.Tianjin General Surgery Institute, Tianjin Medical University General Hospital, Tianjin, 300052, China. ; Bo Shao:1.Department of General Surgery, Tianjin Medical University General Hospital, 154 Anshan Road, Heping District, Tianjin, 300052, China. ; 2
期刊:CD73 mediates the therapeutic effects of endometrial regenerative cells in concanavalin A-induced hepatitis by regulating CD4+ T cells
DOI:10.1186/s13287-023-03505-2
影响因子 :7.5
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Regulation of serotonin production by specific microbes from piglet gut (2023-08-05)
作者:Ziyu Liu:1.Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China. ; 2.National Center for International Research on Animal Gut Nutrition, National Experimental Teaching Demonstration Center of Animal Science, Nanjing Agricultural University, Nanjing, China. ; Yidan Ling:1.Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China. ; 2.National Center for International Research on Animal Gut Nutrition, National Experimental Teaching Demonstration Center of Animal Science, Nanjing Agricultural University, Nanjing, China. ; Yu Peng:1.Hubei CAT Biological Technology Co., Ltd., Wuhan, China. ; Shuibing Han:1.Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China. ; 2.National Center for International Research on Animal Gut Nutrition, National Experimental
期刊:Regulation of serotonin production by specific microbes from piglet gut
DOI:10.1186/s40104-023-00903-7
影响因子 :7.0
引用产品: DMEM/F12 培养基 , RPMI-1640 培养基 , 0.25%胰蛋白酶溶液 (含EDTA,溶于PBS)
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Silicon Nitride Bioceramics Sintered by Microwave Exhibit Excellent Mechanical Properties, Cytocompatibility In Vitro, and Anti-Bacterial Properties (2023-11-17)
作者:Jiayu He:1.Key Laboratory of Biohydrometallurgy of Ministry of Education, School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China. ; Yuandong Liu:1.Key Laboratory of Biohydrometallurgy of Ministry of Education, School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China. ; Xiaofeng Zeng:1.Hengyang Kaixin Special Material Technology Co., Ltd., Hengyang 421200, China. ; 2.Faculty of Engineering, University Malaysia Sabah, Kota Kinabalu 88400, Malaysia. ; Yan Tong:1.Key Laboratory of Biohydrometallurgy of Ministry of Education, School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China. ; Run Liu:1.Key Laboratory of Biohydrometallurgy of Ministry of Education, School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China. ; Kan Wang:1.Key Laboratory of Biohydrometallurgy of Ministry of Education, School of Minerals Processing a
期刊:Silicon Nitride Bioceramics Sintered by Microwave Exhibit Excellent Mechanical Properties, Cytocompatibility In Vitro, and Anti-Bacterial Properties
影响因子 :4.8
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Network pharmacology and experimental validation of Maxing Shigan decoction in the treatment of influenza virus-induced ferroptosis (2023-10-23)
作者:Jiawang Huang:1.College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha 410208, China. ; Xinyue Ma:1.College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha 410208, China. ; Zexuan Liao:1.College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha 410208, China. ; Zhuolin Liu:1.College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha 410208, China. ; Kangyu Wang:1.College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410208, China. ; Zhiying Feng:1.College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410208, China. ; Yi Ning:1.The Medicine School of Hunan University of Chinese Medicine, Changsha 410208, China. ; Fangguo Lu:1.The Medicine School of Hunan University of Chinese Medicine, Changsha 410208
期刊:Network pharmacology and experimental validation of Maxing Shigan decoction in the treatment of influenza virus-induced ferroptosis
DOI:10.1016/S1875-5364(23)60457-1
影响因子 :4.6
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Fractionation of Simulated Digestive Products of Bird’s Nest In Vitro: Skin Whitening Activity of Fractions (2023-08-25)
作者:College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China
期刊:Fractionation of Simulated Digestive Products of Bird’s Nest In Vitro: Skin Whitening Activity of Fractions
影响因子 :4.0
引用产品: RPMI-1640 培养基 , 特级胎牛血清 , Dulbecco's磷酸盐缓冲液 (DPBS),不含钙、镁、酚红 , 0.25%胰蛋白酶溶液 (含EDTA,溶于PBS) , B16-F10 细胞
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