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产品概述
产品简介
大鼠骨髓间充质干细胞成骨诱导分化培养基专门为大鼠骨髓间充质干细胞成骨诱导分化而开发，针对大鼠骨髓间充质干细胞的特性优化分化试剂的配方，可增加大鼠骨髓间充质干细胞的成骨分化效果。
本产品含血清成分，仅用于科研用途，不可用于诊断、治疗、临床及其他用途。
大鼠骨髓间充质干细胞成骨诱导分化培养基组成成分：
| 成分名称 | 添加体积 |
成骨分化专用基础培养基 Basal Medium For Stem Cells Osteogenic Differentiation | 177 mL |
成骨分化专用胎牛血清(FBS) Fetal Bovine Serum For Stem Cells Osteogenic Differentiation | 20 mL |
大鼠骨髓间充质干细胞成骨分化添加物 Supplement For rBMSCs Osteogenic Differentiation | 3 mL |
染色液成分：
茜素红S染色液 Alizarin Red S | 10 mL |
明胶包被液 Gelatin Solution | 10 mL |
注：各成分请根据试剂管上标签标示温度保存。
产品说明
| 产品规格 | 200mL |
| 储存条件 | 按标签所示温度保存，避光储存 |
| 运输条件 | 冰袋冷藏运输 |
| 有效期 | 一年 |
注意事项
1. 由于成骨诱导分化实验历时较长，请在配制及使用本产品过程中严格注意无菌操作。
2. 成骨诱导实验过程中，很难通过镜下观察/白光照片判断是否已经完成诱导，而孔板一经染色又无法继续诱导。因此建议“实验伊始，在另外的孔板上多做(1-3)份平行实验，或仅将‘正常诱导组’在另外的孔板上多做(1-3)份，以便诱导后期用茜素红染色来判断正式实验的诱导进度”。
3. 大鼠骨髓间充质干细胞成骨分化添加物的颜色为白色至黄色，不影响正常使用；
4. 大鼠骨髓间充质干细胞成骨分化添加物融化后有轻微白色沉淀，该成分为L-谷氨酰胺，可将成骨分化 添加物直接添加到基础培养基中，不影响正常使用。
5.血清离心：血清中可能会存在白色絮状沉淀物，这通常是胆固醇、脂肪酸酯以及一些蛋白质析出导致，属于正常现象，该现象不影响细胞的培养和诱导分化。若您欲去除这些絮状沉淀物，可以将血清分装至无菌离心管内，以400-600 g离心5 min，取上清液使用。但是我们不建议您以过滤的方法去除这些絮状沉淀物，一方面它可能会阻塞您的过滤膜；另一方面，过滤血清这种行为可能会导致血清中部分营养成分的流失。
参考文献
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COL6A3 enhances the osteogenic differentiation potential of BMSCs by promoting mitophagy in the osteoporotic microenvironment (2024-01-25)
作者：Kun Wang:1.Department of Orthopedics, Zhongda Hospital, Southeast University, Nanjing, China. ; 2.Medical School of Southeast University, Nanjing, China. ; 3.School of Biomedical Engineering and Informatics, Nanjing Medical University, Nanjing, China. ; Xin Peng:1.Medical School of Southeast University, Nanjing, China. ; Rui Zhang:1.Medical School of Southeast University, Nanjing, China. ; Xiaotao Wu:1.Department of Orthopedics, Zhongda Hospital, Southeast University, Nanjing, China. wuxiaotaospine@seu.edu.cn. ; 2.Medical School of Southeast University, Nanjing, China. wuxiaotaospine@seu.edu.cn. ; Lu Mao:1.Department of Orthopedics, Zhongda Hospital, Southeast University, Nanjing, China. bobai0008@163.com. ; 2.Medical School of Southeast University, Nanjing, China. bobai0008@163.com. ;
期刊：COL6A3 enhances the osteogenic differentiation potential of BMSCs by promoting mitophagy in the osteoporotic microenvironment
DOI：10.1007/s11033-023-08918-z
影响因子 ：2.8
引用产品： 大鼠骨髓间充质干细胞成骨诱导分化培养基 , 大鼠骨髓间充质干细胞成脂诱导分化培养基 , 大鼠骨髓间充质干细胞成软骨诱导分化培养基
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A comprehensive stem cell laboratory module with blended learning for medical students at Tongji University (2024-01-08)
作者：Jie Xu:1.Teaching Laboratory Center, Tongji University School of Medicine, Shanghai, China. ; Zhihua Shao:1.Department of Cell Biology, Tongji University School of Medicine, Shanghai, China. ; Song Jia:1.Teaching Laboratory Center, Tongji University School of Medicine, Shanghai, China. ; Jihong Sha:1.Department of Cell Biology, Tongji University School of Medicine, Shanghai, China. ; Jiao Li:1.Teaching Laboratory Center, Tongji University School of Medicine, Shanghai, China. ; Furong Gao:1.Department of Biochemistry and Molecular Biology, Tongji University School of Medicine, Shanghai, China. ; Xiujuan Shi:1.Department of Biochemistry and Molecular Biology, Tongji University School of Medicine, Shanghai, China. ; Juan Wang:1.Department of Biochemistry and Molecular Biology, Tongji University School of Medicine, Shanghai, China. ; Caixia Jin:1.Department of Biochemistry and Molecular Biology, Tongji University School of Medicine, Shanghai, China. ; Mei Jiang:1.Department of Bio
期刊：A comprehensive stem cell laboratory module with blended learning for medical students at Tongji University
影响因子 ：1.4
引用产品： 大鼠骨髓间充质干细胞成骨诱导分化培养基 , 大鼠骨髓间充质干细胞成脂诱导分化培养基 , 大鼠骨髓间充质干细胞成软骨诱导分化培养基
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Development of an integrated device utilizing exosome-hyaluronic acid-based hydrogel and investigation of its osteogenic and angiogenic characteristics (2023-12-10)
作者：Longbiao Wan: 1.Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan 430060, China; Yan He: 1.Institute for Regenerative and Translational Research, Department of Stomatology, Tianyou Hospital of Wuhan University of Science and Technology, Wuhan 430060, China; Ao Wang: 1.Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan 430060, China; Jiali Pan: 1.Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan 430060, China; Chun Xu: 1.School of Dentistry, The University of Queensland, Herston, QLD 4006, Australia; Dehao Fu: 1.Department of Orthopaedics, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200233, China; Qingsong Ye: 1.Center of Regenerative Medicine, Department of Stomatology, Renmin Hospital of Wuhan University, 460030 Wuhan, Hubei, China; Fei Wu: 1.Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan 430060, China;
期刊：Development of an integrated device utilizing exosome-hyaluronic acid-based hydrogel and investigation of its osteogenic and angiogenic characteristics
DOI：10.1016/j.matdes.2023.112565
影响因子 ：8.4
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Long Non-Coding RNAs Within Macrophage-Derived Exosomes Promote BMSC Osteogenesis in a Bone Fracture Rat Model (2023-02-27)
作者：Dong Wang:1.Department of Orthopedics, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100020, People's Republic of China. ; Yang Liu:1.Department of Orthopedics, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100020, People's Republic of China. ; Shuo Diao:1.Department of Orthopedics, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100020, People's Republic of China. ; Lei Shan:1.Department of Orthopedics, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100020, People's Republic of China. ; Junlin Zhou:1.Department of Orthopedics, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100020, People's Republic of China. ;
期刊：Long Non-Coding RNAs Within Macrophage-Derived Exosomes Promote BMSC Osteogenesis in a Bone Fracture Rat Model
影响因子 ：8.0
引用产品： 大鼠骨髓间充质干细胞成骨诱导分化培养基 , 大鼠骨髓间充质干细胞成脂诱导分化培养基 , 大鼠骨髓间充质干细胞成软骨诱导分化培养基
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Effect of La3+ and Mg2+ combined system on bioactivity and osteogenesis of bioinspired La-doped magnesium phosphate composites prepared utilizing the precursor method (2023-05-19)
作者：Qiaoyun Liu: 1.Department of Orthopedics Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, 430071, China; Yifeng Yu: 1.Department of Orthopedics Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, 430071, China; Changjiang Liu: 1.Department of Orthopedics Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, 430071, China; Yuhang Liu: 1.Department of Orthopedics Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, 430071, China; Liangjie Yuan: 1.College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei, 430072, China; Zheng Wang: 1.Department of Orthopedics Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, 430071, China; Aixi Yu: 1.Department of Orthopedics Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, 430071, China;
期刊：Effect of La3+ and Mg2+ combined system on bioactivity and osteogenesis of bioinspired La-doped magnesium phosphate composites prepared utilizing the precursor method
DOI：10.1016/j.jmrt.2023.05.133
影响因子 ：6.4
引用产品： 大鼠骨髓间充质干细胞 , 大鼠骨髓间充质干细胞完全培养基 , 大鼠骨髓间充质干细胞成骨诱导分化培养基
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Bioinspired strontium magnesium phosphate cement prepared utilizing the precursor method for bone tissue engineering (2023-02-03)
作者：Qiaoyun Liu:1.Department of Orthopedics Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. ; Changjiang Liu:1.Department of Orthopedics Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. ; Weixing Wang:1.Department of Orthopedics Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. ; Liangjie Yuan:1.College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei, China. ; Yu Wang:1.Department of Orthopedics Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. ; 2.16th Department, Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China. ; Xinzeyu Yi:1.Department of Orthopedics Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. ; Zhenyu Pan:1.Department of Orthopedics Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. ; A
期刊：Bioinspired strontium magnesium phosphate cement prepared utilizing the precursor method for bone tissue engineering
DOI：10.3389/fbioe.2023.1142095
影响因子 ：5.7
引用产品： 大鼠骨髓间充质干细胞成骨诱导分化培养基 , 大鼠骨髓间充质干细胞
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Inokosterone activates the BMP2 to promote the osteogenic differentiation of bone marrow mesenchymal stem cells and improve bone loss in ovariectomized rats (2023-10-09)
作者：Qiang Chen:1.Department of Orthopedics, Affiliated Xiaoshan Hospital, Hangzhou Normal University, Hangzhou, 311200, China. ; Zhihua Yang:1.Department of Orthopedics, Affiliated Xiaoshan Hospital, Hangzhou Normal University, Hangzhou, 311200, China. ; Xiangyi Sun:1.Department of Orthopedics, Affiliated Xiaoshan Hospital, Hangzhou Normal University, Hangzhou, 311200, China. ; Ruchao Long:1.Department of Orthopedics, Affiliated Xiaoshan Hospital, Hangzhou Normal University, Hangzhou, 311200, China. ; Jianwei Shen:1.Department of Orthopedics, Affiliated Xiaoshan Hospital, Hangzhou Normal University, Hangzhou, 311200, China. ; Zhen Wang:1.Department of Orthopedics, Affiliated Xiaoshan Hospital, Hangzhou Normal University, Hangzhou, 311200, China. Electronic address: wangzhenyundong@163.com. ;
期刊：Inokosterone activates the BMP2 to promote the osteogenic differentiation of bone marrow mesenchymal stem cells and improve bone loss in ovariectomized rats
DOI：10.1016/j.bbrc.2023.10.032
影响因子 ：3.1
引用产品： 大鼠骨髓间充质干细胞成骨诱导分化培养基
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LncRNA urothelial cancer associated 1 promotes the osteogenic differentiation of human periodontal ligament stem cells by regulating the miR-96-5p/Osx axis (2023-11-28)
作者：Xuefei Sun:1.Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an, China; Clinical Research Center of Shaanxi Province for Dental and Maxillofacial Diseases, Department of Endodontics, College of Stomatology, Xi'an Jiaotong University, Xi'an, China. Electronic address: sunxuefei@xjtu.edu.cn. ; Yu Pu:1.Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an, China; Clinical Research Center of Shaanxi Province for Dental and Maxillofacial Diseases, Department of Endodontics, College of Stomatology, Xi'an Jiaotong University, Xi'an, China. ; Shaojie Dong:1.Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an, China; Clinical Research Center of Shaanxi Province for Dental and Maxillofacial Diseases, Department of Endodontics, College of Sto
期刊：LncRNA urothelial cancer associated 1 promotes the osteogenic differentiation of human periodontal ligament stem cells by regulating the miR-96-5p/Osx axis
DOI：10.1016/j.archoralbio.2023.105855
影响因子 ：3.0
引用产品： 大鼠骨髓间充质干细胞成骨诱导分化培养基
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Exosomal miR-192-5p secreted by bone marrow mesenchymal stem cells inhibits hepatic stellate cell activation and targets PPP2R3A (2023-05-25)
作者：Jie Tan:1.Department of Gastroenterology, Wuhan Third Hospital, Tongren Hospital of Wuhan University, Wuhan, China. ; Mingtao Chen:1.Department of Gastroenterology, Wuhan Third Hospital, Tongren Hospital of Wuhan University, Wuhan, China. ; Meng Liu:1.Department of Gastroenterology, Wuhan Third Hospital, Tongren Hospital of Wuhan University, Wuhan, China. ; Aifang Chen:1.Department of Gastroenterology, Wuhan Third Hospital, Tongren Hospital of Wuhan University, Wuhan, China. ; Min Huang:1.Department of Gastroenterology, Wuhan Third Hospital, Tongren Hospital of Wuhan University, Wuhan, China. ; Xiaoli Chen:1.Department of Gastroenterology, Wuhan Third Hospital, Tongren Hospital of Wuhan University, Wuhan, China. ; Xia Tian:1.Department of Gastroenterology, Wuhan Third Hospital, Tongren Hospital of Wuhan University, Wuhan, China. ; Wei Chen:1.Department of Gastroenterology, Wuhan Third Hospital, Tongren Hospital of Wuhan University, Wuhan, China. ;
期刊：Exosomal miR-192-5p secreted by bone marrow mesenchymal stem cells inhibits hepatic stellate cell activation and targets PPP2R3A
DOI：10.1080/01478885.2023.2215151
影响因子 ：1.1
引用产品： HSC-T6 细胞 , 大鼠骨髓间充质干细胞 , DMEM高糖 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗)，100× , 大鼠骨髓间充质干细胞完全培养基 , 大鼠骨髓间充质干细胞成骨诱导分化培养基 , 大鼠骨髓间充质干细胞成脂诱导分化培养基
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Hyaluronic acid oligosaccharides modified mineralized collagen and chitosan with enhanced osteoinductive properties for bone tissue engineering (2002-01-20)
作者：Min Li:1.National Glycoengineering Research Center, Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, Qingdao, 266200, People's Republic of China. ; Weibin Jia:1.National Glycoengineering Research Center, Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, Qingdao, 266200, People's Republic of China. ; Xiuli Zhang:1.National Glycoengineering Research Center, Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, Qingdao, 266200, People's Republic of China. ; Hongjuan Weng:1.National Glycoengineering Research Center, Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, Qingdao, 266200, People's Republic of China. ; Guofeng Gu:1.National Glycoengineering Research Center, Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, Qingdao, 266200, People's Re
期刊：Hyaluronic acid oligosaccharides modified mineralized collagen and chitosan with enhanced osteoinductive properties for bone tissue engineering
DOI：10.1016/j.carbpol.2021.117780
影响因子 ：11.2
引用产品： 大鼠骨髓间充质干细胞成骨诱导分化培养基
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