产品中心Cell Resources
联系我们CONTACT US
400-999-210024小时服务热线
产品概述
产品简介
人骨髓间充质干细胞成骨诱导分化培养基专门为人骨髓间充质干细胞成骨诱导分化而开发，针对人骨髓间充质干细胞的特性优化分化试剂的配方，可增加人骨髓间充质干细胞的成骨分化效果。
本产品含血清成分，仅用于科研用途，不可用于诊断、治疗、临床及其他用途。
人骨髓间充质干细胞成骨诱导分化培养基组成成分：
| 成分名称 | 添加体积 |
成骨分化专用基础培养基 Basal Medium For Stem Cells Osteogenic Differentiation | 177 mL |
成骨分化专用胎牛血清(FBS) Fetal Bovine Serum For Stem Cells Osteogenic Differentiation | 20 mL |
人骨髓间充质干细胞成骨分化添加物 Supplement For hBMSCs Osteogenic Differentiation | 3 mL |
染色液成分：
茜素红S染色液 Alizarin Red S | 10 mL |
明胶包被液 Gelatin Solution | 10 mL |
注：各成分请根据试剂管上标签标示温度保存。
产品说明
| 产品规格 | 200mL |
| 储存条件 | 按标签所示温度保存，避光储存 |
| 运输条件 | 冰袋冷藏运输 |
| 有效期 | 一年 |
注意事项
1. 由于成骨诱导分化实验历时较长，请在配制及使用本产品过程中严格注意无菌操作。
2. 成骨诱导实验过程中，很难通过镜下观察/白光照片判断是否已经完成诱导，而孔板一经染色又无法继续诱导。因此建议“实验伊始，在另外的孔板上多做(1-3)份平行实验，或仅将‘正常诱导组’在另外的孔板上多做(1-3)份，以便诱导后期用茜素红染色来判断正式实验的诱导进度”。
3. 人骨髓间充质干细胞成骨分化添加物的颜色为白色至黄色，不影响正常使用；
4. 人骨髓间充质干细胞成骨分化添加物融化后有轻微白色沉淀，该成分为L-谷氨酰胺，可将成骨分化 添加物直接添加到基础培养基中，不影响正常使用。
5. 血清离心：血清中可能会存在白色絮状沉淀物，这通常是胆固醇、脂肪酸酯以及一些蛋白质析出导致，属于正常现象，该现象不影响细胞的培养和诱导分化。若您欲去除这些絮状沉淀物，可以将血清分装至无菌离心管内，以400-600 g离心5 min，取上清液使用。但是我们不建议您以过滤的方法去除这些絮状沉淀物，一方面它可能会阻塞您的过滤膜；另一方面，过滤血清这种行为可能会导致血清中部分营养成分的流失。
参考文献
-
A graphene oxide-loaded processed pyritum composite hydrogel for accelerated bone regeneration via mediation of M2 macrophage polarization (2023-07-30)
作者：Changcan Shi:1.School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China. ; Yinting Yu:1.School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China. ; Hongjuan Wu:1.The First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, 210023, China. ; 2.Department of Pulmonology, Jiangning Hospital of Traditional Chinese Medicine, Nanjing, 211100, China. ; Huanjin Liu:1.School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China. ; Mengyu Guo:1.School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China. ; Wenxin Wang:1.School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China. ; Dan Wang:1.School of Pharmaceutical Sciences, Tsinghua University, Beijing, 100089, China. ; Chenxu Wei:1.School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China. ; Hao Zhai:1.State Key Laboratory of Natural Medicines, Department of Pharmaceut
期刊：A graphene oxide-loaded processed pyritum composite hydrogel for accelerated bone regeneration via mediation of M2 macrophage polarization
DOI：10.1016/j.mtbio.2023.100753
影响因子 ：8.2
引用产品： 人骨髓间充质干细胞成骨诱导分化培养基
-
Effect of psoralen on the regulation of osteogenic differentiation induced by periodontal stem cell-derived exosomes (2023-06-03)
作者：Jie Yu:1.School of Stomatology, Qingdao University, Qingdao, 266023, China. ; Xiaonan Wu:1.Qingdao Stomatological Hospital Affiliated to Qingdao University, No.17 Dexian Road, Shinan District, Qingdao, 266001, Shandong, China. ; Wenyi Zhang:1.Qingdao Stomatological Hospital Affiliated to Qingdao University, No.17 Dexian Road, Shinan District, Qingdao, 266001, Shandong, China. ; Fuhang Chu:1.Qingdao Stomatological Hospital Affiliated to Qingdao University, No.17 Dexian Road, Shinan District, Qingdao, 266001, Shandong, China. ; Qi Zhang:1.School of Stomatology, Qingdao University, Qingdao, 266023, China. ; Meihua Gao:1.Qingdao Stomatological Hospital Affiliated to Qingdao University, No.17 Dexian Road, Shinan District, Qingdao, 266001, Shandong, China. ; Yingjie Xu:1.Qingdao Stomatological Hospital Affiliated to Qingdao University, No.17 Dexian Road, Shinan District, Qingdao, 266001, Shandong, China. xyjdywe@163.com. ; Yingtao Wu:1.School of Stomatology, Qingdao University, Qingda
期刊：Effect of psoralen on the regulation of osteogenic differentiation induced by periodontal stem cell-derived exosomes
DOI：10.1007/s13577-023-00918-2
影响因子 ：4.3
引用产品： 特级胎牛血清 , 人骨髓间充质干细胞成脂诱导分化培养基 , 磷酸盐缓冲液 (PBS) 1× , 人骨髓间充质干细胞成骨诱导分化培养基
-
VCAM-1 Promotes Angiogenesis of Bone Marrow Mesenchymal Stem Cells Derived from Patients with Trauma-Induced Osteonecrosis of the Femoral Head by Regulating the Apelin/CCN2 Pathway (2023-10-12)
作者：Yiming Shao:1.Department of Orthopedics, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450001, China. ; 2.Center of Stem Cell and Regenerative Medicine, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450001, China. ; 3.Department of Trauma Surgery, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450001, China. ; Lei Sun:1.Center of Stem Cell and Regenerative Medicine, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450001, China. ; Baodong Ma:1.Center of Stem Cell and Regenerative Medicine, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450001, China. ; Ranran Jin:1.Center of Stem Cell and Regenerative Medicine, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450001, China. ; Yueyao Ban:1.Department of Trauma Surgery, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450001, China. ; Ruibo Li:1.Department o
期刊：VCAM-1 Promotes Angiogenesis of Bone Marrow Mesenchymal Stem Cells Derived from Patients with Trauma-Induced Osteonecrosis of the Femoral Head by Regulating the Apelin/CCN2 Pathway
影响因子 ：4.3
引用产品： DMEM高糖 培养基 , 人骨髓间充质干细胞成骨诱导分化培养基
-
The bone mesenchymal stem cell-derived exosomal miR-146a-5p promotes diabetic wound healing in mice via macrophage M1/M2 polarization (2023-10-18)
作者：Xijie Zhou:1.Department of Hand and Microsurgery, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China. Electronic address: 493565783@qq.com. ; Chenhao Ye:1.Department of Hand and Microsurgery, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China. Electronic address: ych20000628@163.com. ; Liangfu Jiang:1.Department of Hand and Microsurgery, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China. Electronic address: jiangliangfu@wmu.edu.cn. ; Xuwei Zhu:1.Department of Hand and Microsurgery, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China. Electronic address: Drzhuxuwei@163.com. ; Feiya Zhou:1.Department of Hand and Microsurgery, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China. Electronic address: feiyazhou@yeah.net. ; Meizi Xia:1.Department of Nephrology, Wenzhou Geriatric Hospital, Wenzhou, 325000, China. Electroni
期刊：The bone mesenchymal stem cell-derived exosomal miR-146a-5p promotes diabetic wound healing in mice via macrophage M1/M2 polarization
影响因子 ：4.1
引用产品： THP-1 细胞 , 人骨髓间充质干细胞成脂诱导分化培养基 , 人骨髓间充质干细胞成骨诱导分化培养基
FAQs
Q：{{item.question}}
A：
产品资料
识别码示意图
