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产品简介
大鼠骨髓间充质干细胞成脂诱导分化培养基专门为大鼠骨髓间充质干细胞成脂诱导分化而开发，针对大鼠骨髓间充质干细胞的特性优化分化试剂的配方，可增加大鼠骨髓间充质干细胞的成脂分化效果。
本产品含血清成分，仅用于科研用途，不可用于诊断、治疗、临床及其他用途。
成脂诱导分化培养基A液：
成分名称 | 添加体积 |
成脂分化专用基础培养基A Basal Medium For Stem Cells Adipogenic Differentiation A | 177 mL |
成脂分化专用胎牛血清（FBS） Fetal Bovine Serum For Stem Cells Adipogenic Differentiation | 20 mL |
大鼠骨髓间充质干细胞成脂分化添加物A① Supplement For rBMSCs Adipogenic Differentiation A① | 2.8 mL |
大鼠骨髓间充质干细胞成脂分化添加物A② Supplement For rBMSCs Adipogenic Differentiation A② | 200 μL |
成脂诱导分化培养基B液：
成分名称 | 添加体积 |
成脂分化专用基础培养基B Basal Medium For Stem Cells Adipogenic Differentiation B | 177.2 mL |
成脂诱导分化专用胎牛血清（FBS） Fetal Bovine Serum For Stem Cells Adipogenic Differentiation | 20 mL |
大鼠骨髓间充质干细胞成脂分化添加物B Supplement For rBMSCs Adipogenic Differentiation B | 2.8 mL |
辅助试剂：
成分名称 | 添加体积 |
油红O染色液 Oil Red O Solution | 10 mL |
明胶包被液 Gelatin Solution | 10 mL |
注：各成分请根据试剂管上标签标示温度保存。
产品说明
| 产品规格 | 400mL |
| 储存条件 | 按标签所示温度保存，避光储存 |
| 运输条件 | 冰袋冷藏运输 |
| 有效期 | 一年 |
注意事项
1.因为培养基的成分较多，请在配制过程中严格注意无菌操作。
2. A、B交替诱导是为了减轻A液中试剂对干细胞的影响，如果您的干细胞状态较好可在前7天先只使用A液进行刺激诱导（中途每2~3天更换新鲜A液），待脂滴快速出现后，再进行两种培养基交替诱导的操作。
3.血清离心：血清中可能会存在白色絮状沉淀物，这通常是胆固醇、脂肪酸酯以及一些蛋白质析出导致，属于正常现象，该现象不影响细胞的培养和诱导分化。若您欲去除这些絮状沉淀物，可以将血清分装至无菌离心管内，以400-600 g离心5 min，取上清液使用。但是我们不建议您以过滤的方法去除这些絮状沉淀物，一方面它可能会阻塞您的过滤膜；另一方面，过滤血清这种行为可能会导致血清中部分营养成分的流失。
参考文献
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COL6A3 enhances the osteogenic differentiation potential of BMSCs by promoting mitophagy in the osteoporotic microenvironment (2024-01-25)
作者：Kun Wang:1.Department of Orthopedics, Zhongda Hospital, Southeast University, Nanjing, China. ; 2.Medical School of Southeast University, Nanjing, China. ; 3.School of Biomedical Engineering and Informatics, Nanjing Medical University, Nanjing, China. ; Xin Peng:1.Medical School of Southeast University, Nanjing, China. ; Rui Zhang:1.Medical School of Southeast University, Nanjing, China. ; Xiaotao Wu:1.Department of Orthopedics, Zhongda Hospital, Southeast University, Nanjing, China. wuxiaotaospine@seu.edu.cn. ; 2.Medical School of Southeast University, Nanjing, China. wuxiaotaospine@seu.edu.cn. ; Lu Mao:1.Department of Orthopedics, Zhongda Hospital, Southeast University, Nanjing, China. bobai0008@163.com. ; 2.Medical School of Southeast University, Nanjing, China. bobai0008@163.com. ;
期刊：COL6A3 enhances the osteogenic differentiation potential of BMSCs by promoting mitophagy in the osteoporotic microenvironment
DOI：10.1007/s11033-023-08918-z
影响因子 ：2.8
引用产品： 大鼠骨髓间充质干细胞成骨诱导分化培养基 , 大鼠骨髓间充质干细胞成脂诱导分化培养基 , 大鼠骨髓间充质干细胞成软骨诱导分化培养基
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A comprehensive stem cell laboratory module with blended learning for medical students at Tongji University (2024-01-08)
作者：Jie Xu:1.Teaching Laboratory Center, Tongji University School of Medicine, Shanghai, China. ; Zhihua Shao:1.Department of Cell Biology, Tongji University School of Medicine, Shanghai, China. ; Song Jia:1.Teaching Laboratory Center, Tongji University School of Medicine, Shanghai, China. ; Jihong Sha:1.Department of Cell Biology, Tongji University School of Medicine, Shanghai, China. ; Jiao Li:1.Teaching Laboratory Center, Tongji University School of Medicine, Shanghai, China. ; Furong Gao:1.Department of Biochemistry and Molecular Biology, Tongji University School of Medicine, Shanghai, China. ; Xiujuan Shi:1.Department of Biochemistry and Molecular Biology, Tongji University School of Medicine, Shanghai, China. ; Juan Wang:1.Department of Biochemistry and Molecular Biology, Tongji University School of Medicine, Shanghai, China. ; Caixia Jin:1.Department of Biochemistry and Molecular Biology, Tongji University School of Medicine, Shanghai, China. ; Mei Jiang:1.Department of Bio
期刊：A comprehensive stem cell laboratory module with blended learning for medical students at Tongji University
影响因子 ：1.4
引用产品： 大鼠骨髓间充质干细胞成骨诱导分化培养基 , 大鼠骨髓间充质干细胞成脂诱导分化培养基 , 大鼠骨髓间充质干细胞成软骨诱导分化培养基
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Development of an integrated device utilizing exosome-hyaluronic acid-based hydrogel and investigation of its osteogenic and angiogenic characteristics (2023-12-10)
作者：Longbiao Wan: 1.Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan 430060, China; Yan He: 1.Institute for Regenerative and Translational Research, Department of Stomatology, Tianyou Hospital of Wuhan University of Science and Technology, Wuhan 430060, China; Ao Wang: 1.Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan 430060, China; Jiali Pan: 1.Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan 430060, China; Chun Xu: 1.School of Dentistry, The University of Queensland, Herston, QLD 4006, Australia; Dehao Fu: 1.Department of Orthopaedics, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200233, China; Qingsong Ye: 1.Center of Regenerative Medicine, Department of Stomatology, Renmin Hospital of Wuhan University, 460030 Wuhan, Hubei, China; Fei Wu: 1.Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan 430060, China;
期刊：Development of an integrated device utilizing exosome-hyaluronic acid-based hydrogel and investigation of its osteogenic and angiogenic characteristics
DOI：10.1016/j.matdes.2023.112565
影响因子 ：8.4
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Long Non-Coding RNAs Within Macrophage-Derived Exosomes Promote BMSC Osteogenesis in a Bone Fracture Rat Model (2023-02-27)
作者：Dong Wang:1.Department of Orthopedics, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100020, People's Republic of China. ; Yang Liu:1.Department of Orthopedics, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100020, People's Republic of China. ; Shuo Diao:1.Department of Orthopedics, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100020, People's Republic of China. ; Lei Shan:1.Department of Orthopedics, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100020, People's Republic of China. ; Junlin Zhou:1.Department of Orthopedics, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100020, People's Republic of China. ;
期刊：Long Non-Coding RNAs Within Macrophage-Derived Exosomes Promote BMSC Osteogenesis in a Bone Fracture Rat Model
影响因子 ：8.0
引用产品： 大鼠骨髓间充质干细胞成骨诱导分化培养基 , 大鼠骨髓间充质干细胞成脂诱导分化培养基 , 大鼠骨髓间充质干细胞成软骨诱导分化培养基
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Micro/nano topological modification of TiO2 nanotubes activates Thy-1 signaling to control osteogenic differentiation of stem cells (2023-12-31)
作者：Li Jinsheng:1.Institute of Biomedical Engineering, College of Medicine, Southwest Jiaotong University, Chengdu 610031, Sichuan, China; Key Laboratory of Advanced Technologies of Materials Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, PR China. ; Deng Qing:1.Institute of Biomedical Engineering, College of Medicine, Southwest Jiaotong University, Chengdu 610031, Sichuan, China. ; Chen Junhao:1.School of Finance and Economics, Xizang Minzu University, Xianyang 712082, PR China. ; Si Qiqi:1.School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China. ; Chen Jieru:1.School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China. ; Yang Liwen:1.Institute of Biomedical Engineering, College of Medicine, Southwest Jiaotong University, Chengdu 610031, Sichuan, China; Key Laboratory of Advanced Technologies of Materials Ministry of Education, School of Materials
期刊：Micro/nano topological modification of TiO2 nanotubes activates Thy-1 signaling to control osteogenic differentiation of stem cells
DOI：10.1016/j.slasd.2023.12.011
影响因子 ：3.1
引用产品： 大鼠骨髓间充质干细胞成脂诱导分化培养基
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Exosomal miR-192-5p secreted by bone marrow mesenchymal stem cells inhibits hepatic stellate cell activation and targets PPP2R3A (2023-05-25)
作者：Jie Tan:1.Department of Gastroenterology, Wuhan Third Hospital, Tongren Hospital of Wuhan University, Wuhan, China. ; Mingtao Chen:1.Department of Gastroenterology, Wuhan Third Hospital, Tongren Hospital of Wuhan University, Wuhan, China. ; Meng Liu:1.Department of Gastroenterology, Wuhan Third Hospital, Tongren Hospital of Wuhan University, Wuhan, China. ; Aifang Chen:1.Department of Gastroenterology, Wuhan Third Hospital, Tongren Hospital of Wuhan University, Wuhan, China. ; Min Huang:1.Department of Gastroenterology, Wuhan Third Hospital, Tongren Hospital of Wuhan University, Wuhan, China. ; Xiaoli Chen:1.Department of Gastroenterology, Wuhan Third Hospital, Tongren Hospital of Wuhan University, Wuhan, China. ; Xia Tian:1.Department of Gastroenterology, Wuhan Third Hospital, Tongren Hospital of Wuhan University, Wuhan, China. ; Wei Chen:1.Department of Gastroenterology, Wuhan Third Hospital, Tongren Hospital of Wuhan University, Wuhan, China. ;
期刊：Exosomal miR-192-5p secreted by bone marrow mesenchymal stem cells inhibits hepatic stellate cell activation and targets PPP2R3A
DOI：10.1080/01478885.2023.2215151
影响因子 ：1.1
引用产品： HSC-T6 细胞 , 大鼠骨髓间充质干细胞 , DMEM高糖 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗)，100× , 大鼠骨髓间充质干细胞完全培养基 , 大鼠骨髓间充质干细胞成骨诱导分化培养基 , 大鼠骨髓间充质干细胞成脂诱导分化培养基
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CHNQD-00603 Promotes Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells by the miR-452-3p-Mediated Autophagy Pathway. (2012-01-20)
作者：Shanshan Xin:1.Department of Oral and Maxillofacial Surgery, The Affiliated Hospital of Qingdao University, Qingdao, China. ; 2.School of Stomatology, Qingdao University, Qingdao, China. ; Shao-Ming Li:1.Department of Oral and Maxillofacial Surgery, The Affiliated Hospital of Qingdao University, Qingdao, China. ; 2.School of Stomatology, Qingdao University, Qingdao, China. ; Ling Gao:1.Department of Oral and Maxillofacial Surgery, The Affiliated Hospital of Qingdao University, Qingdao, China. ; 2.Key Lab of Oral Clinical Medicine, The Affiliated Hospital of Qingdao University, Qingdao, China. ; Jing-Jing Zheng:1.Department of Endodontics, The Affiliated Hospital of Qingdao University, Qingdao, China. ; Yan-Wei Wu:1.Key Laboratory of Marine Drugs, The Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China, Qingdao, China. ; Chang-Lun Shao:1.Key Laboratory of Marine Drugs, The Ministry of Education of China, School of Medicine and Pharmacy, Ocean
期刊：CHNQD-00603 Promotes Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells by the miR-452-3p-Mediated Autophagy Pathway.
影响因子 ：5.5
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