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产品简介
人脐带间充质干细胞成脂诱导分化培养基专门为人脐带间充质干细胞成脂诱导分化而开发，针对人脐带间充质干细胞的特性优化分化试剂的配方，可增加人脐带间充质干细胞的成脂分化效果。
本产品含血清成分，仅用于科研用途，不可用于诊断、治疗、临床及其他用途。
成脂诱导分化培养基A液：
成分名称 | 添加体积 |
成脂分化专用基础培养基A Basal Medium For Stem Cells Adipogenic Differentiation A | 177 mL |
成脂分化专用胎牛血清（FBS） Fetal Bovine Serum For Stem Cells Adipogenic Differentiation | 20 mL |
人脐带间充质干细胞成脂分化添加物A① Supplement For hUCMSCs Adipogenic Differentiation A① | 2.8 mL |
人脐带间充质干细胞成脂诱导分化添加物A② Supplement For hUCMSCs Adipogenic Differentiation A② | 200 μL |
成脂诱导分化培养基B液：
成分名称 | 添加体积 |
成脂分化专用基础培养基B Basal Medium For Stem Cells Adipogenic Differentiation B | 177.2 mL |
成脂分化专用胎牛血清（FBS） Fetal Bovine Serum For Stem Cells Adipogenic Differentiation | 20 mL |
人脐带间充质干细胞成脂分化添加物B Supplement For hUCMSCs Adipogenic Differentiation B | 2.8 mL |
辅助试剂：
成分名称 | 添加体积 |
油红O染色液 Oil Red O Solution | 10 mL |
明胶包被液 Gelatin Solution | 10 mL |
注：各成分请根据试剂管上标签标示温度保存。
产品说明
| 产品规格 | 400mL |
| 储存条件 | 按标签所示温度保存，避光储存 |
| 运输条件 | 冰袋冷藏运输 |
| 有效期 | 一年 |
注意事项
1.因为培养基的成分较多，请在配制过程中严格注意无菌操作。
2. A、B交替诱导是为了减轻A液中试剂对干细胞的影响，如果您的干细胞状态较好可在前7天先只使用A液进行刺激诱导（中途每2~3天更换新鲜A液），待脂滴快速出现后，再进行两种培养基交替诱导的操作。
3.血清离心：血清中可能会存在白色絮状沉淀物，这通常是胆固醇、脂肪酸酯以及一些蛋白质析出导致，属于正常现象，该现象不影响细胞的培养和诱导分化。若您欲去除这些絮状沉淀物，可以将血清分装至无菌离心管内，以400-600 g离心5 min，取上清液使用。但是我们不建议您以过滤的方法去除这些絮状沉淀物，一方面它可能会阻塞您的过滤膜；另一方面，过滤血清这种行为可能会导致血清中部分营养成分的流失。
参考文献
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Human amniotic MSCs-mediated anti-inflammation of CD206hiIL-10hi macrophages alleviates isoproterenol-induced ventricular remodeling in mice (2024-02-13)
作者：Qi-Ming Huang:1.College of Life Science, Nanchang University, Nanchang 330031, Jiangxi, China; The National Engineering Research Center for Bioengineering Drugs and the Technologies, Institute of Translational Medicine, Nanchang University, Nanchang 330031, Jiangxi, China. ; Ying-Lin Long:1.The National Engineering Research Center for Bioengineering Drugs and the Technologies, Institute of Translational Medicine, Nanchang University, Nanchang 330031, Jiangxi, China. ; Jia-Nan Wang:1.The National Engineering Research Center for Bioengineering Drugs and the Technologies, Institute of Translational Medicine, Nanchang University, Nanchang 330031, Jiangxi, China. ; Jie Wu:1.The National Engineering Research Center for Bioengineering Drugs and the Technologies, Institute of Translational Medicine, Nanchang University, Nanchang 330031, Jiangxi, China. ; Wen-Long Tang:1.The National Engineering Research Center for Bioengineering Drugs and the Technologies, Institute of Translational Medici
期刊：Human amniotic MSCs-mediated anti-inflammation of CD206hiIL-10hi macrophages alleviates isoproterenol-induced ventricular remodeling in mice
DOI：10.1016/j.intimp.2024.111660
影响因子 ：5.6
引用产品： 人脐带间充质干细胞成脂诱导分化培养基 , 人脐带间充质干细胞成骨诱导分化培养基 , 人脐带间充质干细胞成软骨诱导分化培养基
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Cerium oxide nanoparticles-carrying human umbilical cord mesenchymal stem cells counteract oxidative damage and facilitate tendon regeneration (2023-10-03)
作者：Xunshan Ren:1.Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, China. ; Huangming Zhuang:1.Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, China. ; Yuelong Zhang:1.Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, China. ; Panghu Zhou:1.Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, China. zhoupanghu@whu.edu.cn. ;
期刊：Cerium oxide nanoparticles-carrying human umbilical cord mesenchymal stem cells counteract oxidative damage and facilitate tendon regeneration
DOI：10.1186/s12951-023-02125-5
影响因子 ：10.2
引用产品： 人脐带间充质干细胞成脂诱导分化培养基 , 人脐带间充质干细胞成骨诱导分化培养基 , 人脐带间充质干细胞成软骨诱导分化培养基
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MKL-1 suppresses ferroptosis by activating system Xc- and increasing glutathione synthesis (2023-08-21)
作者：Zhou-Tong Dai:1.Department of Gynaecological Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. ; 2.National Clinical Research Centre for Obstetrics and Gynaecology, Cancer Biology Research Centre (Key Laboratory of the Ministry of Education), Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. ; 3.Institute of Biology and Medicine, College of Life and Health Science, Wuhan University of Science and Technology, Wuhan, China. ; Yong-Lin Wu:1.Department of Thyroid and Breast Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. ; Xing-Rui Li:1.Department of Thyroid and Breast Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. ; Xing-Hua Liao:1.Institute of Biology and Medicine, College of Life and Health Science, Wuhan University of Science and Technology, Wuha
期刊：MKL-1 suppresses ferroptosis by activating system Xc- and increasing glutathione synthesis
影响因子 ：9.2
引用产品： 人脐带血间充质干细胞完全培养基 , 人脐带间充质干细胞成骨诱导分化培养基 , 人脐带间充质干细胞成脂诱导分化培养基
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Regulatory mechanism of DHRS2-modified human umbilical cord mesenchymal stem cells-derived exosomes in prostate cancer cell proliferation and apoptosis (2023-03-24)
作者：Xiao Wu:1.Department of Urology, The Second Xiangya Hospital, Central South University, No.139 Renmin Road, Changsha, Hunan Province, 410011, China. ; Zhongyi Zeng:1.Department of Urology, The Second Xiangya Hospital, Central South University, No.139 Renmin Road, Changsha, Hunan Province, 410011, China. ; Kai Peng:1.Department of Urology, The Second Xiangya Hospital, Central South University, No.139 Renmin Road, Changsha, Hunan Province, 410011, China. ; Da Ren:1.Department of Urology, The Second Xiangya Hospital, Central South University, No.139 Renmin Road, Changsha, Hunan Province, 410011, China. ; Lei Zhang:1.Department of Urology, The Second Xiangya Hospital, Central South University, No.139 Renmin Road, Changsha, Hunan Province, 410011, China. Electronic address: zhanglei001@csu.edu.cn. ;
期刊：Regulatory mechanism of DHRS2-modified human umbilical cord mesenchymal stem cells-derived exosomes in prostate cancer cell proliferation and apoptosis
DOI：10.1016/j.tice.2023.102078
影响因子 ：2.6
引用产品： DMEM高糖完全培养基 (含10%FBS） , 人脐带间充质干细胞成骨诱导分化培养基 , 人脐带间充质干细胞成脂诱导分化培养基
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