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产品概述
| 名称 | SK-MEL-1 (人皮肤黑色素瘤细胞) (STR鉴定正确) |
| 别称 | SK-Mel-1; SK Mel 1; SK-Mel 1; SK-Mel1; SKMEL-1; SkMEL-1; SKMEL1; SK 1 |
| 种属 | 人 |
| 生长特性 | 悬浮细胞 |
| 细胞形态 | 球形 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
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| 推荐传代比例 | 3×10^5-5×10^5cells/mL |
| 推荐换液频率 | 2-3次/周 |
| 注意事项 | 该细胞为悬浮细胞，请注意离心收集细胞悬液；请勿直接倒掉细胞培养液。 |
| 背景描述 | SK-MEL-1细胞由Oettgen·F及其同事从一名29岁的患有广泛、快速进展性恶性黑色素瘤的白人男性患者的胸导管中分离建立的。SK-MEL-1细胞可产生黑色素，电镜检测发现SK-MEL-1细胞中色素颗粒与自身合成和吞噬作用相关。在63%的恶性黑色素瘤患者和10%其他疾病患者体内发现了针对SK-MEL-1细胞的抗体。 |
| 年龄（性别） | 男性；29岁 |
| 组织来源 | 恶性黑色素瘤；皮肤；源自转移部位：淋巴系统 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 黑色素瘤细胞 |
| 生物安全等级 | BSL-1 |
| 倍增时间 | ~100 hours |
| 致瘤性 | Yes, in nude mice; forms pigmented malignant melanomas; also forms tumors in the cheek pouch of cortisone treated hamsters. |
| 抗原表达情况 | Blood Type A; Rh+ |
| 保藏机构 | ATCC; HTB-67 DSMZ; ACC-303 |
STR鉴定
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STR位点信息
Amelogenin X,Y CSF1PO 12,13 D2S1338 19,23 D3S1358 14,16 D5S818 12,13 D7S820 12 D8S1179 13,16 D13S317 11 D16S539 11,12 D18S51 13,16 D19S433 15,16.2 D21S11 29,32.2 FGA 18,20 PentaD 11,13 PentaE 7,21 TH01 6 TPOX 11 vWA 16,17 D6S1043 11 D12S391 18,20 D2S441 11 -
STR鉴定图
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参考文献
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Anti-proliferative and pro-apoptotic effects of curcumin on skin cutaneous melanoma: Bioinformatics analysis and in vitro experimental studies. (2022-09-12)
作者：Long Li:1.Department of Ophthalmology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China. ; Shuwen Lu:1.Department of Ophthalmology, the First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou, China. ; Chao Ma:1.Department of Ophthalmology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China. ;
期刊：Anti-proliferative and pro-apoptotic effects of curcumin on skin cutaneous melanoma: Bioinformatics analysis and in vitro experimental studies.
影响因子 ：4.8
引用产品： SK-MEL-1 细胞
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USP22 deficiency in melanoma mediates resistance to T cells through IFNγ-JAK1-STAT1 signal axis (2002-01-20)
作者：Min Li:1.Sheng Yushou Center of Cell Biology and Immunology, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, People's Republic of China; Joint International Research Laboratory of Metabolic & Developmental Sciences, Shanghai Jiao Tong University, Shanghai, People's Republic of China. ; Yanqin Xu:1.Sheng Yushou Center of Cell Biology and Immunology, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, People's Republic of China; Joint International Research Laboratory of Metabolic & Developmental Sciences, Shanghai Jiao Tong University, Shanghai, People's Republic of China. ; Jie Liang:1.Sheng Yushou Center of Cell Biology and Immunology, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, People's Republic of China. ; Hao Lin:1.Sheng Yushou Center of Cell Biology and Immunology, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, People's Republic of Chi
期刊：USP22 deficiency in melanoma mediates resistance to T cells through IFNγ-JAK1-STAT1 signal axis
DOI：10.1016/j.ymthe.2021.02.018
影响因子 ：12.4
引用产品： A875 [A-875] 细胞 , SK-MEL-1 细胞
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PURPL represses autophagic cell death to promote cutaneous melanoma by modulating ULK1 phosphorylation (2011-01-20)
作者：Shuo Han:1.Department of Toxicology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, China. ; Xue Li:1.Department of Toxicology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, China. ; 2.School of Clinical Medicine and Technology, Sichuan Vocational College of Health and Rehabilitation, Zigong, China. ; Ke Wang:1.Department of Toxicology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, China. ; Dingheng Zhu:1.Dermatology Hospital, Southern Medical University, Guangzhou, China. ; Bingyao Meng:1.Department of Toxicology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, China. ; Jieyu Liu:1.Department of Toxicology, Guangdong Provincial Key Laboratory of Tro
期刊：PURPL represses autophagic cell death to promote cutaneous melanoma by modulating ULK1 phosphorylation
DOI：10.1038/s41419-021-04362-8
影响因子 ：9.0
引用产品： SK-MEL-1 细胞
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