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产品概述
名称 | A-375 (人恶性黑色素瘤细胞) (STR鉴定正确) |
别称 | A 375; A375; A375-MEL; A375-mel; A375mel |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:3-1:6 |
推荐换液频率 | 2-3次/周 |
背景描述 | A-375细胞源自一位54岁女性,是由D·J·Giard等人建立的一系列细胞株中的一株。A-375细胞在抗胸腺细胞球蛋白、血清处理的NIH瑞士小鼠中形成类似于恶性黑素瘤的快速增长的皮下肿瘤;A-375在普通成纤维细胞和琼脂上形成克隆。 |
年龄(性别) | 女性;54岁 |
组织来源 | 皮肤;恶性黑色素瘤 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 黑色素瘤细胞 |
生物安全等级 | BSL-1 |
倍增时间 | ~24-32 hours |
致瘤性 | Yes, in immunosuppressed mice. |
保藏机构 | ATCC; CRL-1619 ATCC; CRL-7904BCRC; 60039 BCRJ; 0278 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 11,12 D2S1338 16,24 D3S1358 15,17 D5S818 12 D7S820 9 D8S1179 11,14 D13S317 11,14 D16S539 9 D18S51 12,17 D19S433 13,14.2 D21S11 29,30 FGA 23 PentaD 9,15 PentaE 10,12 TH01 8 TPOX 8,10 vWA 16,17 D6S1043 11,14 D12S391 18,21 D2S441 11 -
STR鉴定图
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参考文献
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A novel mitochondria-targeting DHODH inhibitor induces robust ferroptosis and alleviates immune suppression (2024-02-27)
期刊:PHARMACOLOGICAL RESEARCH
DOI:10.1016/j.phrs.2024.107115
影响因子 :9.3
引用产品: B16-F10 细胞 , A-375 细胞 , 4T1 细胞 , Renca [RenCa] 细胞 , GL261 细胞 , U-87 MG 细胞 , SH-SY5Y [SHSY-5Y] 细胞 , U251 细胞
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YY1-induced lncRNA00511 promotes melanoma progression via the miR-150-5p/ADAM19 axis (2024-02-15)
期刊:American Journal of Cancer Research
DOI:PMID:38455406
影响因子 :5.3
引用产品: A-375 细胞 , SK-MEL-28 细胞 , 人表皮黑色素细胞
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Envoplakin Inhibits Macrophage Polarization by Altering the Inflammatory Tumor Microenvironment of Melanoma Through the RAS / ERK Signaling Pathway (2024-03-14)
期刊:Journal of Inflammation Research
影响因子 :4.5
引用产品: A-375 细胞 , A2058 细胞 , A875 [A-875] 细胞 , SK-MEL-28 细胞
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LPCAT1 promotes melanoma cell proliferation via Akt signaling (2024-03-26)
期刊:ONCOLOGY REPORTS
影响因子 :4.2
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Cholesterol removal improves performance of a model biomimetic system to co-deliver a photothermal agent and a STING agonist for cancer immunotherapy (2023-08-22)
期刊:Nature Communications
DOI:10.1038/s41467-023-40814-9
影响因子 :16.6
引用产品: CTLL-2 细胞 , A-375 细胞 , LLC [LL/2; LLC1] 细胞 , RAW 264.7 细胞
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Multivalent DNA Flowers for High-Performance Isolation, Detection, and Release of Tumor-Derived Extracellular Vesicles (2023-11-26)
期刊:ACS Applied Materials & Interfaces
影响因子 :9.5
引用产品: MCF7 [MCF-7] 细胞 , A-375 细胞 , MCF 10A 细胞 , NCI-H1975 细胞 , A549 [A-549] 细胞
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Design, Synthesis, and Biological Evaluation of Proteolysis-Targeting Chimeras as Highly Selective and Efficient Degraders of Extracellular Signal-Regulated Kinase 5 (2023-09-26)
期刊:JOURNAL OF MEDICINAL CHEMISTRY
DOI:10.1021/acs.jmedchem.3c00864
影响因子 :7.3
引用产品: A-375 细胞 , HCT 116 细胞 , Hep G2 细胞 , MDA-MB-231 细胞 , NCI-H1975 细胞 , PC-3 细胞 , THP-1 细胞
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Development and validation of ferroptosis-related lncRNA signature and immune-related gene signature for predicting the prognosis of cutaneous melanoma patients (2023-03-24)
期刊:APOPTOSIS
DOI:10.1007/s10495-023-01831-7
影响因子 :7.2
引用产品: A-375 细胞
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RNF8 enhances the sensitivity of PD-L1 inhibitor against melanoma through ubiquitination of galectin-3 in stroma (2023-06-30)
期刊:Cell Death Discovery
DOI:10.1038/s41420-023-01500-3
影响因子 :7.0
引用产品: A-375 细胞 , A549 [A-549] 细胞 , B16-F10 细胞 , 293T [HEK-293T] 细胞
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Evaluation of the Effect of Fibroblasts on Melanoma Metastasis Using a Biomimetic Co-Culture Model (2023-04-07)
期刊:ACS Biomaterials Science & Engineering
DOI:10.1021/acsbiomaterials.2c01186
影响因子 :5.8
引用产品: A-375 细胞
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PTPRC functions as a prognosis biomarker in the tumor microenvironment of cutaneous melanoma (2023-11-23)
期刊:Scientific Reports
DOI:10.1038/s41598-023-46794-6
影响因子 :4.6
引用产品: A-375 细胞 , SK-MEL-28 细胞 , 青霉素-链霉素溶液 (双抗),100×
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Retinoblastoma-Binding Protein 5 Regulates H3K4 Methylation Modification to Inhibit the Proliferation of Melanoma Cells by Inactivating the Wnt/β-Catenin and Epithelial-Mesenchymal Transition Pathways (2023-02-21)
期刊:Journal of Oncology
影响因子 :4.5
引用产品: A-375 细胞
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Identification of CRKL as an oncogenic biomarker for prognosis and immunotherapy in melanoma, and its potential molecular mechanism (2023-04-28)
期刊:GENOMICS
DOI:10.1016/j.ygeno.2023.110634
影响因子 :4.4
引用产品: Hacat 细胞 , A-375 细胞 , SK-MEL-5 细胞
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MitoCur-1 induces ferroptosis to reverse vemurafenib resistance in melanoma through inhibition of USP14 (2023-11-20)
期刊:Pigment Cell & Melanoma Research
影响因子 :4.3
引用产品: A-375 细胞 , SK-MEL-28 细胞
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Immune infiltration and clinical significance analyses of the cancer-associated fibroblast-related signature in skin cutaneous melanoma (2023-10-17)
期刊:JOURNAL OF GENE MEDICINE
DOI:10.1002/jgm.3614
影响因子 :3.5
引用产品: A-375 细胞 , SK-Mel-5 细胞
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LSM2 is associated with a poor prognosis and promotes cell proliferation, migration, and invasion in skin cutaneous melanoma (2023-06-13)
期刊:BMC Medical Genomics
DOI:10.1186/s12920-023-01564-1
影响因子 :2.7
引用产品: A2058 细胞 , A-375 细胞 , MeWo 细胞 , SK-MEL-2 细胞 , SK-MEL-28 细胞 , 人表皮黑色素细胞
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LncRNA FENDRR Suppresses Melanoma Growth via Influencing c-Myc mRNA Level (2023-08-08)
期刊:Clinical Cosmetic and Investigational Dermatology
影响因子 :2.3
引用产品: A-375 细胞
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Design, Synthesis, and Molecular Docking Study of Novel 3-Cyanopyridine Derivatives for the Anti-Cancer Drug Target Survivin Protein (2023-03-01)
期刊:Medicinal Chemistry
DOI:10.2174/1573406418666220829160820
影响因子 :2.3
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Human EVI2B acts as a Janus-faced oncogene/antioncogene by differently affecting as per cancer type neoplastic cells growth and immune infiltration (2023-03-23)
期刊:Oncologie
DOI:10.1515/oncologie-2022-1002
影响因子 :0.9
引用产品: U-937 细胞 , Jurkat, Clone E6-1 细胞 , A549 [A-549] 细胞 , A-375 细胞 , Hep G2 细胞 , U-87 MG 细胞
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The Krüppel-like factor 6 represses proliferation and invasion of cutaneous malignant melanoma (2023-10-25)
期刊:POLISH JOURNAL OF PATHOLOGY
影响因子 :0.8
引用产品: A-375 细胞 , DMEM高糖 培养基
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6-Shogaol Exhibits a Promoting Effect with Tax via Binding HSP60 in Non-Small-Cell Lung Cancer (2022-11-19)
期刊:Cells
影响因子 :7.7
引用产品: A-375 细胞 , A549 [A-549] 细胞 , H8 细胞 , U251 细胞 , 3T3-L1 细胞 , PANC-1 细胞 , TSSCA 细胞 , HK-2 细胞 , DMEM高糖 培养基 , RPMI-1640 培养基
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Promotion of Melanoma Cell Proliferation by Cyclic Straining through Regulatory Morphogenesis (2022-10-06)
期刊:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
影响因子 :6.2
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Identification and characterization of two novel non-coding Tyrosinase (TYR) gene variants leading to Oculocutaneous albinism type 1 (2004-02-20)
期刊:JOURNAL OF BIOLOGICAL CHEMISTRY
影响因子 :4.8
引用产品: 293T [HEK-293T] 细胞 , A-375 细胞 , B16-F10 细胞
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miR-767-3p suppresses melanoma progression by inhibiting ASF1B expression (2022-08-09)
期刊:BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
DOI:10.1016/j.bbrc.2022.08.014
影响因子 :3.3
引用产品: A875 [A-875] 细胞 , A-375 细胞 , A2058 细胞 , RPMI-1640 培养基 , DMEM高糖 培养基 , 特级胎牛血清
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Dual Modal Imaging-Guided Drug Delivery System for Combined Chemo-Photothermal Melanoma Therapy (2005-01-20)
期刊:International Journal of Nanomedicine
影响因子 :8.0
引用产品: A-375 细胞
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Structures and antitumor activities of ten new and twenty known surfactins from the deep-sea bacterium Limimaricola sp. SCSIO 53532 (2021-12-31)
期刊: BIOORGANIC CHEMISTRY
DOI:10.1016/j.bioorg.2021.105589
影响因子 :5.1
引用产品: A-375 细胞 , HCT 116 细胞 , MDA-MB-231 细胞 , A549 [A-549] 细胞
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LRRK2 correlates with macrophage infiltration in pan-cancer (2021-12-18)
期刊:Genomics
DOI:10.1016/j.ygeno.2021.11.037
影响因子 :4.4
引用产品: A-375 细胞
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Acetone Extract of Cornus officinalis Leaves Exerts Anti-Melanoma Effects via Inhibiting STAT3 Signaling (2005-01-20)
期刊:OncoTargets and Therapy
影响因子 :4.3
引用产品: A-375 细胞
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Extraction and Separation of Sinapine from Rapeseed Cake and the Mode of Action of Melanin Production Inhibition (2001-01-20)
期刊:Molecular Biology
影响因子 :1.2
引用产品: A-375 细胞
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A Machine Learning Method for Drug Combination Prediction (2008-00-20)
期刊:Frontiers in Genetics
影响因子 :3.7
引用产品: A-375 细胞
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The Antitumor Potential of Extract of the Oak Bracket Medicinal Mushroom Inonotus baumii in SMMC-7721 Tumor Cells (2009-09-20)
期刊:Evidence-based Complementary and Alternative Medicine
影响因子 :2.7
引用产品: SMMC-7721 细胞 , A-375 细胞 , U-87 MG 细胞 , MDA-MB-231 细胞 , SK-OV-3 细胞
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Naringin suppresses the metabolism of A375 cells by inhibiting the phosphorylation of c-Src (2010-05-20)
期刊:Tumor Biology
影响因子 :0.0
引用产品: A-375 细胞 , A875 [A-875] 细胞
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