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产品概述
名称 | A-375 (人恶性黑色素瘤细胞) (STR鉴定正确) |
别称 | A 375; A375; A375-MEL; A375-mel; A375mel |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:3-1:6 |
推荐换液频率 | 2-3次/周 |
背景描述 | A-375细胞源自一位54岁女性,是由D·J·Giard等人建立的一系列细胞株中的一株。A-375细胞在抗胸腺细胞球蛋白、血清处理的NIH瑞士小鼠中形成类似于恶性黑素瘤的快速增长的皮下肿瘤;A-375在普通成纤维细胞和琼脂上形成克隆。 |
年龄(性别) | 女性;54岁 |
组织来源 | 皮肤;恶性黑色素瘤 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 黑色素瘤细胞 |
生物安全等级 | BSL-1 |
倍增时间 | ~24-32 hours |
致瘤性 | Yes, in immunosuppressed mice. |
保藏机构 | ATCC; CRL-1619 ATCC; CRL-7904BCRC; 60039 BCRJ; 0278 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 11,12 D2S1338 16,24 D3S1358 15,17 D5S818 12 D7S820 9 D8S1179 11,14 D13S317 11,14 D16S539 9 D18S51 12,17 D19S433 13,14.2 D21S11 29,30 FGA 23 PentaD 9,15 PentaE 10,12 TH01 8 TPOX 8,10 vWA 16,17 D6S1043 11,14 D12S391 18,21 D2S441 11 -
STR鉴定图
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参考文献
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Chemically engineered antibodies for autophagy-based receptor degradation (2025-01-09)
期刊:Nature Chemical Biology
DOI:10.1038/s41589-024-01803-1
影响因子 :12.9
引用产品: Hela 细胞 , MDA-MB-231 细胞 , U-87 MG 细胞 , A549 [A-549] 细胞 , PANC-1 细胞 , NCI-H292 细胞 , NCI-H1299 细胞 , NCI-H1975 细胞 , A-375 细胞 , HuH-7 细胞 , 293T [HEK-293T] 细胞
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Visualization of carboxylesterase 2 regulation in kidney injury via an enzyme-activated fluorescent probe (2025-01-22)
期刊:SENSORS AND ACTUATORS B-CHEMICAL
影响因子 :8.0
引用产品: SW480 [SW-480] 细胞 , MCF7 [MCF-7] 细胞 , A-375 细胞 , HL-7702 [L-02; LO2] 细胞 , Hep G2 细胞
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The anti-melanoma roles and mechanisms of tricholoma isoflavone derivative CA028 (2025-01-09)
期刊:npj Science of Food
DOI:10.1038/s41538-025-00370-6
影响因子 :6.3
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RET Inhibitor SPP86 Triggers Apoptosis and Activates the DNA Damage Response Through the Suppression of Autophagy and the PI3K/AKT Signaling Pathway in Melanoma Cells (2025-01-07)
期刊:Drug Design Development and Therapy
影响因子 :4.7
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Lasalocid inhibits melanoma by down-regulating FOXM1 through PI3K/AKT and JNK/P38 MAPK pathways (2025-01-01)
期刊:Journal of Cancer
影响因子 :3.3
引用产品: A-375 细胞 , SK-MEL-28 细胞
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Chemical Constituents of the Deep-Sea-Derived Fungus Trichoderma simmonsii ZEN3 (2025-01-25)
期刊:CHEMISTRY & BIODIVERSITY
影响因子 :2.3
引用产品: A-375 细胞
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D3S-001, a KRAS G12C inhibitor with rapid target engagement kinetics, overcomes nucleotide cycling and demonstrates robust preclinical and clinical activities (2024-05-08)
期刊:Cancer Discovery
DOI:10.1158/2159-8290.CD-24-0006
影响因子 :28.2
引用产品: AGS 细胞 , A549 [A-549] 细胞 , A-375 细胞 , KYSE-410 细胞 , NCI-H358 细胞 , AsPC-1 细胞 , BxPC-3 细胞 , HCT-15 细胞 , HCT 116 细胞 , SW480 [SW-480] 细胞 , SW620 细胞
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Advanced Nanoencapsulation-Enabled Ultrasensitive Analysis: Unraveling Tumor Extracellular Vesicle Subpopulations for Differential Diagnosis of Hepatocellular Carcinoma via DNA Cascade Reactions (2024-04-17)
期刊:ACS Nano
影响因子 :15.8
引用产品: Hep G2 细胞 , SK-Hep-1 细胞 , HuH-7 细胞 , HL-7702 [L-02; LO2] 细胞 , Hela 细胞 , MCF7 [MCF-7] 细胞 , A-375 细胞
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Electrical Stimulation Promotes Endocytosis of Magnetic Nanoparticles by Cancer Cells (2024-08-10)
期刊:Small
影响因子 :13.0
引用产品: MG-63 细胞 , MCF7 [MCF-7] 细胞 , U-87 MG 细胞 , A-375 细胞 , TCCSUP 细胞
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Mucin 18-targeted humanized monoclonal antibody immune positron emission tomography imaging and patient-derived tumor xenograft visualization (2024-11-07)
期刊:VIEW
影响因子 :9.7
引用产品: SK-MEL-28 细胞 , A549 [A-549] 细胞 , HMVII 细胞 , A-375 细胞
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A novel mitochondria-targeting DHODH inhibitor induces robust ferroptosis and alleviates immune suppression (2024-02-27)
期刊:PHARMACOLOGICAL RESEARCH
DOI:10.1016/j.phrs.2024.107115
影响因子 :9.3
引用产品: B16-F10 细胞 , A-375 细胞 , 4T1 细胞 , Renca [RenCa] 细胞 , GL261 细胞 , U-87 MG 细胞 , SH-SY5Y [SHSY-5Y] 细胞 , U251 细胞
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Local anesthetic tetracaine hydrochloride induces pyroptosis via caspase-3/gasdermin E in uveal melanoma (2024-09-24)
期刊:BIOMEDICINE & PHARMACOTHERAPY
DOI:10.1016/j.biopha.2024.117471
影响因子 :6.9
引用产品: A-375 细胞 , HMY-1 细胞 , C918 细胞 , 293T [HEK-293T] 细胞 , A2058 细胞
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Heating conversion of indole-3-carbinol into N-substituted oligomers with anti-melanoma effect (2024-04-24)
期刊:Food Chemistry-X
DOI:10.1016/j.fochx.2024.101410
影响因子 :6.1
引用产品: A-375 细胞
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Discovery of novel 2-substituted 2, 3-dihydroquinazolin-4(1H)-one derivatives as tubulin polymerization inhibitors for anticancer therapy: The in vitro and in vivo biological evaluation (2024-08-13)
期刊:EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
DOI:10.1016/j.ejmech.2024.116766
影响因子 :6.0
引用产品: A549 [A-549] 细胞 , Hep G2 细胞 , U251 细胞 , PANC-1 细胞 , A-375 细胞
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Integrative Gut Microbiota and Metabolomic Analyses Reveal the PANoptosis- and Ferroptosis-Related Mechanisms of Chrysoeriol in Inhibiting Melanoma (2024-11-04)
期刊:JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
影响因子 :5.7
引用产品: 青霉素-链霉素溶液(双抗),100× , B16-F10 细胞 , A-375 细胞 , 特级胎牛血清
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Feature Matching of Microsecond-Pulsed Magnetic Fields Combined with Fe3O4 Particles for Killing A375 Melanoma Cells (2024-04-26)
期刊:Biomolecules
影响因子 :5.5
引用产品: A-375 细胞
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Cytotoxic and Antibacterial Meroterpenoids Isolated from the Marine-Derived Fungus Talaromyces sp. M27416 (2024-04-20)
期刊:Marine Drugs
影响因子 :5.4
引用产品: K-562 [K562] 细胞 , TE-1 细胞 , HOS 细胞 , GBC-SD 细胞 , PaTu 8988t 细胞 , DU 145 细胞 , 293T [HEK-293T] 细胞 , SF126 细胞 , MCF7 [MCF-7] 细胞 , HCT 116 细胞 , Hela 细胞 , A549 [A-549] 细胞 , Hep G2 细胞 , A-375 细胞 , MKN-45 细胞 , 5637 细胞 , 786-O [786-0] 细胞 , CAL-62 细胞 , A-673 细胞 , HL-7702 [L-02; LO2] 细胞
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YY1-induced lncRNA00511 promotes melanoma progression via the miR-150-5p/ADAM19 axis (2024-02-15)
期刊:American Journal of Cancer Research
DOI:PMID:38455406
影响因子 :5.3
引用产品: A-375 细胞 , SK-MEL-28 细胞 , 人表皮黑色素细胞
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A peptide encoded by LINC00944 suppresses the growth of melanoma cells by diminishing EP400-MYC interaction (2024-11-24)
期刊:BIOCHEMICAL PHARMACOLOGY
影响因子 :5.3
引用产品: A-375 细胞 , A875 [A-875] 细胞 , 293T [HEK-293T] 细胞
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Inhibition of Autophagy by Berbamine Hydrochloride Mitigates Tumor Immune Escape by Elevating MHC-I in Melanoma Cells (2024-09-13)
期刊:Cells
影响因子 :5.1
引用产品: A-375 细胞
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Promotion of apoptosis in melanoma cells by taxifolin through the PI3K/AKT signaling pathway: Screening of natural products using WGCNA and CMAP platforms (2024-06-25)
期刊:INTERNATIONAL IMMUNOPHARMACOLOGY
DOI:10.1016/j.intimp.2024.112517
影响因子 :4.8
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Envoplakin Inhibits Macrophage Polarization by Altering the Inflammatory Tumor Microenvironment of Melanoma Through the RAS / ERK Signaling Pathway (2024-03-14)
期刊:Journal of Inflammation Research
影响因子 :4.5
引用产品: A-375 细胞 , A2058 细胞 , A875 [A-875] 细胞 , SK-MEL-28 细胞
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LPCAT1 promotes melanoma cell proliferation via Akt signaling (2024-03-26)
期刊:ONCOLOGY REPORTS
影响因子 :4.2
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IFN-γ-responsiveness of lymphatic endothelial cells inhibits melanoma lymphatic dissemination via AMPK-mediated metabolic control (2024-06-25)
期刊:BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
DOI:10.1016/j.bbadis.2024.167314
影响因子 :4.2
引用产品: A-375 细胞
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Preliminary Exploration of the Biophysical Mechanisms of Pulsed Magnetic Field-Induced Cell Permeabilization (2024-04-16)
期刊:IEEE TRANSACTIONS ON NANOBIOSCIENCE
影响因子 :3.9
引用产品: A-375 细胞
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DCLRE1B as a novel prognostic biomarker associated with immune infiltration: a pancancer analysis (2024-12-30)
期刊:Scientific Reports
DOI:10.1038/s41598-024-80603-y
影响因子 :3.8
引用产品: A-375 细胞 , DMEM高糖 培养基
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Oxygen Vacancy-Enriched CoFe2O4 for Electrochemically Sensitive Detection of the Breast Cancer CD44 Biomarker (2024-07-05)
期刊:LANGMUIR
DOI:10.1021/acs.langmuir.4c01496
影响因子 :3.7
引用产品: A-375 细胞
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RNF144A-AS1 stabilizes TAF15 and promotes malignant biological behaviors of skin cutaneous melanoma (2024-06-15)
期刊:MOLECULAR AND CELLULAR BIOCHEMISTRY
DOI:10.1007/s11010-024-05045-6
影响因子 :3.5
引用产品: 特级胎牛血清 , SK-MEL-28 细胞 , HFF-1 细胞 , A875 [A-875] 细胞 , A-375 细胞 , SK-MEL-1 细胞 , 青霉素-链霉素溶液(双抗),100×
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Identification and validation of matrix metalloproteinase hub genes as potential biomarkers for Skin Cutaneous Melanoma (2024-10-18)
期刊:Frontiers in Oncology
影响因子 :3.5
引用产品: HS294T 细胞 , UACC-257 细胞 , CCD-8Sk 细胞 , HMCB 细胞 , CCD-1152Sk 细胞 , WM1552C 细胞 , Malme-3M 细胞 , Hs 895.P 细胞 , SK-MEL-3 细胞 , Hs 895.R 细胞 , MeWo 细胞 , WM35 细胞 , SK-MEL-5 细胞 , LOX IMVI 细胞 , CCD-1106 KERTr 细胞 , Hs 895.A 细胞 , Hs 895.C 细胞 , Hs 895.Sk 细胞 , G361 细胞 , A-375 细胞 , IPC-298 细胞 , Hs 888.Sk 细胞 , Hs 895.O 细胞 , CCD-1112Sk 细胞 , YUGEN8 细胞 , CCD-1121Sk 细胞 , CCD-1140Sk 细胞 , Hs 852.T 细胞 , C32 细胞 , UACC-62 细胞 , Hs 895.D 细胞 , Hs 936.Sk 细胞 , RPMI-7951 细胞 , Hs 895.B 细胞 , SK-MEL-28 细胞 , SK-MEL-2 细胞 , A2058 细胞 , Hs 919.Sk 细胞 , WM793 细胞 , HS-27A 细胞 , Hs 895.T 细胞
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Integration of single-cell and bulk RNA sequencing data reveals that CYTOR is a potential prognostic and immunotherapeutic response marker for skin cutaneous melanoma (2024-05-28)
期刊:Journal of Cancer
影响因子 :3.3
引用产品: A875 [A-875] 细胞 , A-375 细胞
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Developing a prognostic model for skin melanoma based on the persistent tumor mutation burden and determining IL17REL as a therapeutic target (2024-06-20)
期刊:JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
DOI:10.1007/s00432-024-05843-x
影响因子 :2.7
引用产品: A875 [A-875] 细胞 , A-375 细胞
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Lnc NEAT1 facilitates the progression of melanoma by targeting the miR-152-3p/CDK6 axis: An observational study (2024-11-01)
期刊:MEDICINE
DOI:10.1097/MD.0000000000040379
影响因子 :1.3
引用产品: Hacat 细胞 , A-375 细胞
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Cholesterol removal improves performance of a model biomimetic system to co-deliver a photothermal agent and a STING agonist for cancer immunotherapy (2023-08-22)
期刊:Nature Communications
DOI:10.1038/s41467-023-40814-9
影响因子 :16.6
引用产品: CTLL-2 细胞 , A-375 细胞 , LLC [LL/2; LLC1] 细胞 , RAW 264.7 细胞
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Blue-ringed octopus-inspired microneedle patch for robust tissue surface adhesion and active injection drug delivery (2023-06-21)
期刊:Science Advances
影响因子 :13.6
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Multivalent DNA Flowers for High-Performance Isolation, Detection, and Release of Tumor-Derived Extracellular Vesicles (2023-11-26)
期刊:ACS Applied Materials & Interfaces
影响因子 :9.5
引用产品: MCF7 [MCF-7] 细胞 , A-375 细胞 , MCF 10A 细胞 , NCI-H1975 细胞 , A549 [A-549] 细胞
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Design, Synthesis, and Biological Evaluation of Proteolysis-Targeting Chimeras as Highly Selective and Efficient Degraders of Extracellular Signal-Regulated Kinase 5 (2023-09-26)
期刊:JOURNAL OF MEDICINAL CHEMISTRY
DOI:10.1021/acs.jmedchem.3c00864
影响因子 :7.3
引用产品: A-375 细胞 , HCT 116 细胞 , Hep G2 细胞 , MDA-MB-231 细胞 , NCI-H1975 细胞 , PC-3 细胞 , THP-1 细胞
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Development and validation of ferroptosis-related lncRNA signature and immune-related gene signature for predicting the prognosis of cutaneous melanoma patients (2023-03-24)
期刊:APOPTOSIS
DOI:10.1007/s10495-023-01831-7
影响因子 :7.2
引用产品: A-375 细胞
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RNF8 enhances the sensitivity of PD-L1 inhibitor against melanoma through ubiquitination of galectin-3 in stroma (2023-06-30)
期刊:Cell Death Discovery
DOI:10.1038/s41420-023-01500-3
影响因子 :7.0
引用产品: A-375 细胞 , A549 [A-549] 细胞 , B16-F10 细胞 , 293T [HEK-293T] 细胞
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Evaluation of the Effect of Fibroblasts on Melanoma Metastasis Using a Biomimetic Co-Culture Model (2023-04-07)
期刊:ACS Biomaterials Science & Engineering
DOI:10.1021/acsbiomaterials.2c01186
影响因子 :5.8
引用产品: A-375 细胞
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PTPRC functions as a prognosis biomarker in the tumor microenvironment of cutaneous melanoma (2023-11-23)
期刊:Scientific Reports
DOI:10.1038/s41598-023-46794-6
影响因子 :4.6
引用产品: A-375 细胞 , SK-MEL-28 细胞 , 青霉素-链霉素溶液 (双抗),100×
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Retinoblastoma-Binding Protein 5 Regulates H3K4 Methylation Modification to Inhibit the Proliferation of Melanoma Cells by Inactivating the Wnt/β-Catenin and Epithelial-Mesenchymal Transition Pathways (2023-02-21)
期刊:Journal of Oncology
影响因子 :4.5
引用产品: A-375 细胞
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Identification of CRKL as an oncogenic biomarker for prognosis and immunotherapy in melanoma, and its potential molecular mechanism (2023-04-28)
期刊:GENOMICS
DOI:10.1016/j.ygeno.2023.110634
影响因子 :4.4
引用产品: Hacat 细胞 , A-375 细胞 , SK-MEL-5 细胞
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MitoCur-1 induces ferroptosis to reverse vemurafenib resistance in melanoma through inhibition of USP14 (2023-11-20)
期刊:Pigment Cell & Melanoma Research
影响因子 :4.3
引用产品: A-375 细胞 , SK-MEL-28 细胞
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Immune infiltration and clinical significance analyses of the cancer-associated fibroblast-related signature in skin cutaneous melanoma (2023-10-17)
期刊:JOURNAL OF GENE MEDICINE
DOI:10.1002/jgm.3614
影响因子 :3.5
引用产品: A-375 细胞 , SK-Mel-5 细胞
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LSM2 is associated with a poor prognosis and promotes cell proliferation, migration, and invasion in skin cutaneous melanoma (2023-06-13)
期刊:BMC Medical Genomics
DOI:10.1186/s12920-023-01564-1
影响因子 :2.7
引用产品: A2058 细胞 , A-375 细胞 , MeWo 细胞 , SK-MEL-2 细胞 , SK-MEL-28 细胞 , 人表皮黑色素细胞
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LncRNA FENDRR Suppresses Melanoma Growth via Influencing c-Myc mRNA Level (2023-08-08)
期刊:Clinical Cosmetic and Investigational Dermatology
影响因子 :2.3
引用产品: A-375 细胞
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Design, Synthesis, and Molecular Docking Study of Novel 3-Cyanopyridine Derivatives for the Anti-Cancer Drug Target Survivin Protein (2023-03-01)
期刊:Medicinal Chemistry
DOI:10.2174/1573406418666220829160820
影响因子 :2.3
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Human EVI2B acts as a Janus-faced oncogene/antioncogene by differently affecting as per cancer type neoplastic cells growth and immune infiltration (2023-03-23)
期刊:Oncologie
DOI:10.1515/oncologie-2022-1002
影响因子 :0.9
引用产品: U-937 细胞 , Jurkat, Clone E6-1 细胞 , A549 [A-549] 细胞 , A-375 细胞 , Hep G2 细胞 , U-87 MG 细胞
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The Krüppel-like factor 6 represses proliferation and invasion of?cutaneous malignant melanoma (2023-10-25)
期刊:POLISH JOURNAL OF PATHOLOGY
影响因子 :0.8
引用产品: A-375 细胞 , DMEM高糖 培养基
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Selective enrichment and detection of PD-L1 positive extracellular vesicles derived from human plasma and patient derived tumor cells (2022-11-28)
期刊:SENSORS AND ACTUATORS B-CHEMICAL
影响因子 :9.2
引用产品: A-375 细胞 , MCF7 [MCF-7] 细胞
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6-Shogaol Exhibits a Promoting Effect with Tax via Binding HSP60 in Non-Small-Cell Lung Cancer (2022-11-19)
期刊:Cells
影响因子 :7.7
引用产品: A-375 细胞 , A549 [A-549] 细胞 , H8 细胞 , U251 细胞 , 3T3-L1 细胞 , PANC-1 细胞 , TSSCA 细胞 , HK-2 细胞 , DMEM高糖 培养基 , RPMI-1640 培养基
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Promotion of Melanoma Cell Proliferation by Cyclic Straining through Regulatory Morphogenesis (2022-10-06)
期刊:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
影响因子 :6.2
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Identification and characterization of two novel non-coding Tyrosinase (TYR) gene variants leading to Oculocutaneous albinism type 1 (2022-04-10)
期刊:JOURNAL OF BIOLOGICAL CHEMISTRY
影响因子 :4.8
引用产品: 293T [HEK-293T] 细胞 , A-375 细胞 , B16-F10 细胞
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A new amide alkaloid induces the apoptosis of human melanoma A375 cells via inhibition of the STAT3 signaling pathway (2022-11-16)
期刊:NEW JOURNAL OF CHEMISTRY
影响因子 :3.9
引用产品: A-375 细胞
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NCAPG2 contributes to the progression of malignant melanoma through regulating proliferation and metastasis (2022-10-20)
期刊:Biochemistry and Cell Biology
影响因子 :3.7
引用产品: A-375 细胞 , SK-MEL-28 细胞
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miR-767-3p suppresses melanoma progression by inhibiting ASF1B expression (2022-08-09)
期刊:BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
DOI:10.1016/j.bbrc.2022.08.014
影响因子 :3.3
引用产品: A875 [A-875] 细胞 , A-375 细胞 , A2058 细胞 , RPMI-1640 培养基 , DMEM高糖 培养基 , 特级胎牛血清
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Guanine nucleotide exchange factor H1 can be a new biomarker of melanoma (2022-12-07)
期刊:Biologics-Targets & Therapy
影响因子 :
引用产品: A-375 细胞 , A875 [A-875] 细胞
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Dual Modal Imaging-Guided Drug Delivery System for Combined Chemo-Photothermal Melanoma Therapy (2021-05-18)
期刊:International Journal of Nanomedicine
影响因子 :8.0
引用产品: A-375 细胞
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Structures and antitumor activities of ten new and twenty known surfactins from the deep-sea bacterium Limimaricola sp. SCSIO 53532 (2021-12-31)
期刊: BIOORGANIC CHEMISTRY
DOI:10.1016/j.bioorg.2021.105589
影响因子 :5.1
引用产品: A-375 细胞 , HCT 116 细胞 , MDA-MB-231 细胞 , A549 [A-549] 细胞
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LRRK2 correlates with macrophage infiltration in pan-cancer (2021-12-18)
期刊:Genomics
DOI:10.1016/j.ygeno.2021.11.037
影响因子 :4.4
引用产品: A-375 细胞
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Acetone Extract of Cornus officinalis Leaves Exerts Anti-Melanoma Effects via Inhibiting STAT3 Signaling (2021-05-28)
期刊:OncoTargets and Therapy
影响因子 :4.4
引用产品: A-375 细胞
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Extraction and Separation of Sinapine from Rapeseed Cake and the Mode of Action of Melanin Production Inhibition (2021-01-05)
期刊:Molecular Biology
影响因子 :1.2
引用产品: A-375 细胞
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A Machine Learning Method for Drug Combination Prediction (2020-08-25)
期刊:Frontiers in Genetics
影响因子 :3.7
引用产品: A-375 细胞
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The Antitumor Potential of Extract of the Oak Bracket Medicinal Mushroom Inonotus baumii in SMMC-7721 Tumor Cells (2019-09-22)
期刊:Evidence-based Complementary and Alternative Medicine
影响因子 :2.7
引用产品: SMMC-7721 细胞 , A-375 细胞 , U-87 MG 细胞 , MDA-MB-231 细胞 , SK-OV-3 细胞
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Naringin suppresses the metabolism of A375 cells by inhibiting the phosphorylation of c-Src (2015-10-17)
期刊:Tumor Biology
影响因子 :0.0
引用产品: A-375 细胞 , A875 [A-875] 细胞
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