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产品概述
名称 | SK-MEL-28 (人恶性黑色素瘤细胞) (STR鉴定正确) |
别称 | SK-Mel-28; SK.MEL.28; SK-MEL 28; SK MEL-28; SK MEL 28; SK Mel 28; SKMel-28; SKMEL-28; SK-MEL28; SK-Mel28; SK Mel28; SKMEL28; SKMel28; SKmel28; SKML-28; SK28; AU-Mel; P-36; P36 |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 多边形 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:3-1:8 |
推荐换液频率 | 2-3天/次 |
年龄(性别) | 男性;51岁 |
组织来源 | 皮肤 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 皮肤黑色素瘤 |
生物安全等级 | BSL-1 |
倍增时间 | 27 hours (PubMed=7718330); 72 hours (PubMed=12479222); 26.9 hours (PubMed=20406486); 35.1 hours (NCI-DTP). |
致瘤性 | Yes; Yes, in nude mice; forms malignant melanoma (large round cell type) |
保藏机构 | AddexBio; C0020001/66 ATCC; HTB-72 BCRJ; 0289 CLS; 300337 ICLC; HTL99010 NCI-DTP; SK-MEL-28 RCB; RCB1930 - Discontinued TKG; TKG 0333 |
STR鉴定
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STR位点信息
Amelogenin X,Y CSF1PO 10 D2S1338 18 D3S1358 16,18 D5S818 13 D7S820 9.3,10,11 D8S1179 13 D13S317 11,12 D16S539 9,12 D18S51 12,16 D19S433 14 D21S11 28,29 FGA 19 PentaD 9,10 PentaE 8,12 TH01 7 TPOX 8,12,13 vWA 16,19 D6S1043 18 D12S391 15,19 D2S441 10,11 -
STR鉴定图
参考文献
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A novel PDPN antagonist peptide CY12-RP2 inhibits melanoma growth via Wnt/β-catenin and modulates the immune cells (2024-01-02)
作者:Chunyan Feng:1.Institute of Biopharmaceutical and Health Engineering, Shenzhen Key Laboratory of Gene and Antibody Therapy, State Key Laboratory of Chemical Oncogenomics, Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, Guangdong, China. ; 2.School of Life Sciences, Tsinghua University, Beijing, 100084, China. ; Albert Yu:1.Institute of Biopharmaceutical and Health Engineering, Shenzhen Key Laboratory of Gene and Antibody Therapy, State Key Laboratory of Chemical Oncogenomics, Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, Guangdong, China. ; Zhongfu Wang:1.Department of Interventional Radiology, Shenzhen People's Hospital, 1017 Dongmen North Road, Shenzhen, 518020, NoGuangdong, China. ; Kun Wang:1.Institute of Biopharmaceutical and Health Engineering, Shenzhen Key Laboratory of Gene and Antibody Therapy, State Key Laboratory of Chemical Oncogenomics, Shenzhen International Graduate School, Tsinghua University, Shenzhen,
期刊:A novel PDPN antagonist peptide CY12-RP2 inhibits melanoma growth via Wnt/β-catenin and modulates the immune cells
DOI:10.1186/s13046-023-02910-y
影响因子 :11.3
引用产品: A875 [A-875] 细胞 , 293T [HEK-293T] 细胞 , SK-MEL-28 细胞
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YY1-induced lncRNA00511 promotes melanoma progression via the miR-150-5p/ADAM19 axis (2024-02-15)
作者:Ya-Ni Chen:1.The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University Hohhot 010020, Inner Mongolia, China. ; Xin-Rui Fu:1.The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University Hohhot 010020, Inner Mongolia, China. ; Hua Guo:1.The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University Hohhot 010020, Inner Mongolia, China. ; Xin-Yao Fu:1.The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University Hohhot 010020, Inner Mongolia, China. ; Ke-Song Shi:1.The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University Hohhot 010020, Inner Mongolia, China. ; Tian Gao:1.The State Key Laborat
期刊:YY1-induced lncRNA00511 promotes melanoma progression via the miR-150-5p/ADAM19 axis
DOI:PMID:38455406
影响因子 :5.3
引用产品: A-375 细胞 , SK-MEL-28 细胞 , 人表皮黑色素细胞
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Envoplakin Inhibits Macrophage Polarization by Altering the Inflammatory Tumor Microenvironment of Melanoma Through the RAS / ERK Signaling Pathway (2024-03-14)
作者:Weilin Cai:1.Medical School of Chinese PLA, Beijing, People's Republic of China. ; 2.Senior Department of Burns and Plastic Surgery, the Fourth Medical Center of PLA General Hospital, Beijing, People's Republic of China. ; Minliang Chen:1.Senior Department of Burns and Plastic Surgery, the Fourth Medical Center of PLA General Hospital, Beijing, People's Republic of China. ;
期刊:Envoplakin Inhibits Macrophage Polarization by Altering the Inflammatory Tumor Microenvironment of Melanoma Through the RAS / ERK Signaling Pathway
影响因子 :4.5
引用产品: A-375 细胞 , A2058 细胞 , A875 [A-875] 细胞 , SK-MEL-28 细胞
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Reverse flow enhanced inertia pinched flow fractionation (2023-09-06)
作者:Saijie Wang:1.Shenzhen Key Laboratory of Smart Healthcare Engineering, Guangdong Provincial Key Laboratory of Advanced Biomaterials, Department of Biomedical Engineering, Southern University of Science and Technology, No. 1088 Xueyuan Rd, Nanshan District, Shenzhen, Guangdong 518055, China. wangd9@sustech.edu.cn. ; Quanchen Xu:1.Shenzhen Key Laboratory of Smart Healthcare Engineering, Guangdong Provincial Key Laboratory of Advanced Biomaterials, Department of Biomedical Engineering, Southern University of Science and Technology, No. 1088 Xueyuan Rd, Nanshan District, Shenzhen, Guangdong 518055, China. wangd9@sustech.edu.cn. ; Zhihan Zhang:1.Shenzhen Key Laboratory of Smart Healthcare Engineering, Guangdong Provincial Key Laboratory of Advanced Biomaterials, Department of Biomedical Engineering, Southern University of Science and Technology, No. 1088 Xueyuan Rd, Nanshan District, Shenzhen, Guangdong 518055, China. wangd9@sustech.edu.cn. ; Shengbo Chen:1.Shenzhen Key Laboratory of Sma
期刊:Reverse flow enhanced inertia pinched flow fractionation
影响因子 :6.1
引用产品: SK-MEL-28 细胞
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PTPRC functions as a prognosis biomarker in the tumor microenvironment of cutaneous melanoma (2023-11-23)
作者:Xuemei Li:1.Department of Dermatology, The Third Xiangya Hospital, Central South University, Changsha, Hunan Province, 410000, People's Republic of China. ; Zhanghui Yue:1.Department of Dermatology, The Third Xiangya Hospital, Central South University, Changsha, Hunan Province, 410000, People's Republic of China. ; Dan Wang:1.Department of Dermatology, The Third Xiangya Hospital, Central South University, Changsha, Hunan Province, 410000, People's Republic of China. 168301013@csu.edu.cnz. ; Lu Zhou:1.Department of Dermatology, The Third Xiangya Hospital, Central South University, Changsha, Hunan Province, 410000, People's Republic of China. 290991732@qq.com. ;
期刊:PTPRC functions as a prognosis biomarker in the tumor microenvironment of cutaneous melanoma
DOI:10.1038/s41598-023-46794-6
影响因子 :4.6
引用产品: A-375 细胞 , SK-MEL-28 细胞 , 青霉素-链霉素溶液 (双抗),100×
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MitoCur-1 induces ferroptosis to reverse vemurafenib resistance in melanoma through inhibition of USP14 (2023-11-20)
作者:Gege Li:1.School of Public Health, Institute of Toxicology, Lanzhou University, Lanzhou, China. ; Changlong Zhou:1.School of Public Health, Institute of Toxicology, Lanzhou University, Lanzhou, China. ; Lu Wang:1.Nuclear Medicine Department, Lanzhou University Second Hospital, Lanzhou, Gansu, China. ; 2.Second Clinical School, Lanzhou University, Lanzhou, China. ; Yalong Zheng:1.State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou, Gansu, China. ; Bo Zhou:1.State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou, Gansu, China. ; Guoyan Li:1.School of Public Health, Institute of Toxicology, Lanzhou University, Lanzhou, China. ; Zhongyu Ma:1.School of Public Health, Institute of Toxicology, Lanzhou University, Lanzhou, China. ; Peng Sun:1.Second Department of Cardio-Thoracic Surgery, Gansu Cancer Hospital, Lanzhou, China. ; Yuantao Deng:1.Department of Anesthesiology, Gansu Cancer Hospital, Lanzhou, China. ; Li Su:1.School of Publ
期刊:MitoCur-1 induces ferroptosis to reverse vemurafenib resistance in melanoma through inhibition of USP14
影响因子 :4.3
引用产品: A-375 细胞 , SK-MEL-28 细胞
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LSM2 is associated with a poor prognosis and promotes cell proliferation, migration, and invasion in skin cutaneous melanoma (2023-06-13)
作者:Xiaofang Sun:1.Department of Dermatology, the Second Affiliated Hospital of Jiaxing University, Jiaxing, Zhejiang, China. ; 2.Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital of Soochow University, Soochow University, Jiangsu, China. ; Jianping Zhang:1.Department of Dermatology, the Second Affiliated Hospital of Jiaxing University, Jiaxing, Zhejiang, China. ; Jiayuan Hu:1.Department of Dermatology, the Second Affiliated Hospital of Jiaxing University, Jiaxing, Zhejiang, China. ; Qingdong Han:1.Department of Dermatology, the Second Affiliated Hospital of Jiaxing University, Jiaxing, Zhejiang, China. ; Zili Ge:1.Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital of Soochow University, Soochow University, Jiangsu, China. sdgzlfy@163.com. ;
期刊:LSM2 is associated with a poor prognosis and promotes cell proliferation, migration, and invasion in skin cutaneous melanoma
DOI:10.1186/s12920-023-01564-1
影响因子 :2.7
引用产品: A2058 细胞 , A-375 细胞 , MeWo 细胞 , SK-MEL-2 细胞 , SK-MEL-28 细胞 , 人表皮黑色素细胞
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