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产品概述
名称 | BALB/3T3 clone A31 (小鼠胚胎成纤维细胞) (STR鉴定正确) |
别称 | BALB/c 3T3 clone A31; Balb/c3T3; Balb/c 3T3; BALB/3T3; Balb/3T3-4-Cl31; 3T3 clone A31; BALB/3T3 cl. A31; BALB 3T3 clone A31; BALB/3T3 (clone A31); B/C3T3; 3T3-A31; 3T3(A31) |
种属 | 小鼠 |
生长特性 | 贴壁细胞 |
细胞形态 | 成纤维细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:2-1:3 |
推荐换液频率 | 2-3次/周 |
背景描述 | BALB/3T3 clone A31细胞是由S·A·Aaronson和G·T·Todaro于1968年从裂解的14-17天的BALB/c小鼠胚胎中建立的几株细胞之一。BALB/3T3 clone A31细胞对接触抑制特别敏感,生长需高倍数稀释,饱和浓度低。此外,BALB/3T3 clone A31细胞对癌基因DNA病毒SV40和小鼠肉瘤病毒高度易感。 |
年龄(性别) | 胚胎,孕14-17天 |
组织来源 | 胚胎;成纤维细胞 |
细胞类型 | 自发永生化细胞 |
生物安全等级 | BSL-1 |
保藏机构 | ATCC; CCL-163 ECACC; 86110401 |
STR鉴定
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STR位点信息
18-3 18 6-7 12 5-5 14 X-1 25 1-2 17 7-1 25.2 8-1 13 1-1 16 19-2 14 15-3 22.3 12-1 16 6-4 18 4-2 21.3 3-2 14 2-1 16 13-1 15.2,16.2
11-2 17 17-2 15,16 -
STR鉴定图
参考文献
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Inhibition of Poly(ADP-ribose) Polymerase Sensitizes [177Lu]Lu-DOTAGA.(SA.FAPi)2-Mediated Radiotherapy in Triple-Negative Breast Cancer (2023-04-17)
作者:Guangfa Bao:1.Department of Nuclear Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan 430074, China. ; Huimin Zhou:1.Department of Nuclear Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan 430074, China. ; Sijuan Zou:1.Department of Nuclear Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan 430074, China. ; Lixing Chen:1.Department of Nuclear Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan 430074, China. ; Buchuan Zhang:1.Department of Nuclear Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan 430074, China. ; Ziqiang Wang:1.Department of Nuclear Medicine, Tongji Hospital, Tongji Medical College, Huazhong Univers
期刊:Inhibition of Poly(ADP-ribose) Polymerase Sensitizes [177Lu]Lu-DOTAGA.(SA.FAPi)2-Mediated Radiotherapy in Triple-Negative Breast Cancer
DOI:10.1021/acs.molpharmaceut.2c01051
影响因子 :4.9
引用产品: BALB/3T3 clone A31 细胞
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Preparation of Conotoxin-Encapsulated Chitosan Nanoparticles and Evaluation of Their Skin Permeability (2023-01-27)
作者:Haigang Li:1.College of Medicine, Linyi University, Linyi, 276000, Shandong, People's Republic of China. ; JiPeng Yao:1.College of Medicine, Linyi University, Linyi, 276000, Shandong, People's Republic of China. ; Yong Guo:1.College of Medicine, Linyi University, Linyi, 276000, Shandong, People's Republic of China. ; JingJing Huo:1.College of Medicine, Linyi University, Linyi, 276000, Shandong, People's Republic of China. ; Haijuan Zhang:1.College of Medicine, Linyi University, Linyi, 276000, Shandong, People's Republic of China. ; Zengtao Zhang:1.Shandong Renrui Biotechnology Inc., RiZhao, Shandong, People's Republic of China. ; Jinlong Zhao:1.Department of Thoracic Surgery, Linyi People's Hospital, Linyi, 276000, Shandong, People's Republic of China. zhaojinlong-125@163.com. ; Chun Zhang:1.College of Medicine, Linyi University, Linyi, 276000, Shandong, People's Republic of China. zhangchun@lyu.edu.cn. ; 2.Shandong Renrui Biotechnology Inc., RiZhao, Shandong, People's Republic
期刊:Preparation of Conotoxin-Encapsulated Chitosan Nanoparticles and Evaluation of Their Skin Permeability
DOI:10.1208/s12249-023-02509-8
影响因子 :3.3
引用产品: BALB/3T3 clone A31 细胞
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Study on properties and biocompatibility of poly (butylene succinate) and sodium alginate biodegradable composites for biomedical applications (2022-08-26)
作者:Medical College, Qingdao University, Qingdao, People's Republic of China
期刊:Study on properties and biocompatibility of poly (butylene succinate) and sodium alginate biodegradable composites for biomedical applications
影响因子 :2.0
引用产品: NIH/3T3 细胞 , BALB/3T3 clone A31 细胞
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Monitoring and Modulating mtDNA G-Quadruplex Dynamics Reveal Its Close Relationship to Cell Glycolysis (2012-01-20)
作者:Xiu-Cai Chen:1.Guangdong Provincial Key Laboratory of New Drug Design and Evaluation, School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, China. ; Gui-Xue Tang:1.Guangdong Provincial Key Laboratory of New Drug Design and Evaluation, School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, China. ; Wen-Hua Luo:1.Guangdong Provincial Key Laboratory of New Drug Design and Evaluation, School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, China. ; Wen Shao:1.Guangdong Provincial Key Laboratory of New Drug Design and Evaluation, School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, China. ; Jing Dai:1.Guangdong Provincial Key Laboratory of New Drug Design and Evaluation, School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, China. ; Shu-Tang Zeng:1.Guangdong Provincial Key Laboratory of New Drug Design and Evaluation, School of Pharmaceutical Sciences, Sun Yat-Sen University
期刊:Monitoring and Modulating mtDNA G-Quadruplex Dynamics Reveal Its Close Relationship to Cell Glycolysis
影响因子 :16.4
引用产品: HFL1 细胞 , Hacat 细胞 , HT22 细胞 , BALB/3T3 clone A31 细胞 , U-87 MG 细胞
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