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产品概述
名称 | GC-2spd(ts) (小鼠精母细胞) (STR鉴定正确) |
别称 | GC-2 |
种属 | 小鼠 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:3-1:6 |
推荐换液频率 | 2-3次/周 |
背景描述 | The cells have lost their differentiation potential, and are currently arrested at a premeiotic stage. |
年龄(性别) | 雄性;6周龄 |
组织来源 | 精母细胞;SV40大T抗原转染 |
细胞类型 | 条件永生化细胞系 |
生物安全等级 | BSL-2 |
应用领域 | This cell line may be useful for the study of spermatogenesis. |
保藏机构 | ATCC; CRL-2196 BCRC; 60313 |
STR鉴定
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STR位点信息
18-3 16,17 6-7 17 5-5 14,17,18,19 X-1 28 1-2 13,19 7-1 26.2,29 8-1 16 1-1 17 19-2 13 15-3 22.3 12-1 17 6-4 18 4-2 20.3 3-2 14 2-1 16 13-1 15.2,17.1
11-2 16 17-2 15 -
STR鉴定图
参考文献
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Gut-Derived Exosomes Mediate the Microbiota Dysbiosis-Induced Spermatogenesis Impairment by Targeting Meioc in Mice (2024-03-25)
作者:Tong Chen:1.College of Animal Sciences, Jilin University, 5333 Xian Road, Changchun, Jilin, 130062, China. ; Boqi Zhang:1.College of Animal Sciences, Jilin University, 5333 Xian Road, Changchun, Jilin, 130062, China. ; Guitian He:1.College of Animal Sciences, Jilin University, 5333 Xian Road, Changchun, Jilin, 130062, China. ; Nan Wang:1.College of Animal Sciences, Jilin University, 5333 Xian Road, Changchun, Jilin, 130062, China. ; Maosheng Cao:1.College of Animal Sciences, Jilin University, 5333 Xian Road, Changchun, Jilin, 130062, China. ; Caomeihui Shen:1.College of Animal Sciences, Jilin University, 5333 Xian Road, Changchun, Jilin, 130062, China. ; Xue Chen:1.College of Animal Sciences, Jilin University, 5333 Xian Road, Changchun, Jilin, 130062, China. ; Lu Chen:1.College of Animal Sciences, Jilin University, 5333 Xian Road, Changchun, Jilin, 130062, China. ; Kening Liu:1.College of Animal Sciences, Jilin University, 5333 Xian Road, Changchun, Jilin, 130062, China. ; Yux
期刊:Gut-Derived Exosomes Mediate the Microbiota Dysbiosis-Induced Spermatogenesis Impairment by Targeting Meioc in Mice
影响因子 :15.1
引用产品: GC-1 spg 细胞 , GC-2spd (ts) 细胞
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The necroptosis-mediated imbalance of mitochondrial dynamics is involved in DEHP-induced toxicity to immature testes via the PGAM5–DRP1 interaction (2024-02-15)
作者:Xiazhu Zhou:1.Department of Urology, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing, 400014, PR China; Chongqing Key Laboratory of Structural Birth Defect and Reconstruction, Chongqing Key Laboratory of Children Urogenital Development and Tissue Engineering, Children's Hospital of Chongqing Medical University, Chongqing, 400014, PR China. ; Yifan Hong:1.Department of Urology, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing, 400014, PR China; Chongqing Key Laboratory of Structural Birth Defect and Reconstruction, Chongqing Key Laboratory of Children Urogenital Development and Tissue Engineering, Children's Hospital of Chongqing Medical University, Chongqing, 400014, PR China. ; Jin
期刊:The necroptosis-mediated imbalance of mitochondrial dynamics is involved in DEHP-induced toxicity to immature testes via the PGAM5–DRP1 interaction
DOI:10.1016/j.envpol.2024.123585
影响因子 :8.9
引用产品: GC-1 spg 细胞 , GC-2spd (ts) 细胞
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Roles of ferroptosis in type 1 diabetes induced spermatogenic dysfunction (2024-02-17)
作者:Yalei Cao:1.Department of Urology, Center for Reproductive Medicine, Peking University Third Hospital, Beijing, China. ; Zirun Jin:1.Department of Urology, Peking University First Hospital, Beijing, China; Institute of Urology, Peking University, Beijing, China; Department of andrology, Peking University First Hospital, Beijing, China. ; Yu Xi:1.Department of Urology, Center for Reproductive Medicine, Peking University Third Hospital, Beijing, China. ; Jianxing Cheng:1.Department of Urology, Center for Reproductive Medicine, Peking University Third Hospital, Beijing, China. ; Zishui Fang:1.Department of Urology, Peking University First Hospital, Beijing, China; Institute of Urology, Peking University, Beijing, China; Department of andrology, Peking University First Hospital, Beijing, China. ; Qiancheng Zhao:1.Department of Urology, Peking University First Hospital, Beijing, China; Institute of Urology, Peking University, Beijing, China; Department of andrology, Peking University F
期刊:Roles of ferroptosis in type 1 diabetes induced spermatogenic dysfunction
DOI:10.1016/j.freeradbiomed.2024.02.006
影响因子 :7.4
引用产品: GC-2spd (ts) 细胞
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Dapagliflozin ameliorates diabetes-induced spermatogenic dysfunction by modulating the adenosine metabolism along the gut microbiota-testis axis (2024-01-05)
作者:Zirun Jin:1.Department of Urology, Center for Reproductive Medicine, Peking University Third Hospital, No. 49 North Garden Road, Haidian District, Beijing, 100191, China. ; 2.Department of Urology, Department of Andrology, Peking University First Hospital, No. 8, Xishiku Street, Xicheng District, Beijing, 100034, China. ; Yalei Cao:1.Department of Urology, Center for Reproductive Medicine, Peking University Third Hospital, No. 49 North Garden Road, Haidian District, Beijing, 100191, China. ; Qi Wen:1.Department of Obstetrics and Gynecology, Center for Reproductive Medicine, State Key Laboratory of Female Fertility Promotion, Peking University Third Hospital, No.49 North Garden Road, Haidian District, Beijing, 100191, China. ; 2.Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing, China. ; 3.Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Beijing, China. ; 4.National Clinical Research Center for Obstetrics and Gynecology, Beijing, Ch
期刊:Dapagliflozin ameliorates diabetes-induced spermatogenic dysfunction by modulating the adenosine metabolism along the gut microbiota-testis axis
DOI:10.1038/s41598-024-51224-2
影响因子 :4.6
引用产品: GC-2spd (ts) 细胞
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The gut metabolite 3-hydroxyphenylacetic acid rejuvenates spermatogenic dysfunction in aged mice through GPX4-mediated ferroptosis (2023-09-27)
作者:Zirun Jin:1.Department of Urology, Center for Reproductive Medicine, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing, 100191, China. ; 2.Department of Urology, Peking University First Hospital, Xishiku Road, Xicheng District, Beijing, 100034, China. ; 3.Institute of Urology, Peking University, Beijing, China. ; 4.Department of Andrology, Peking University First Hospital, Beijing, China. ; Yuzhuo Yang:1.Department of Obstetrics and Gynecology, Peking University First Hospital, Beijing, China. ; Yalei Cao:1.Department of Urology, Center for Reproductive Medicine, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing, 100191, China. ; Qi Wen:1.Department of Obstetrics and Gynecology, State Key Laboratory of Female Fertility Promotion, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing, 100191, China. ; 2.Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing, China. ; 3.Beijing
期刊:The gut metabolite 3-hydroxyphenylacetic acid rejuvenates spermatogenic dysfunction in aged mice through GPX4-mediated ferroptosis
DOI:10.1186/s40168-023-01659-y
影响因子 :15.5
引用产品: GC-2spd (ts) 细胞
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The rare-earth yttrium induces cell apoptosis and autophagy in the male reproductive system through ROS-Ca2+-CamkII/Ampk axis (2023-07-20)
作者:Fangpeng Hou:1.Center for Immunology, Key Laboratory of Prevention and Treatment of Cardiovascular and Cerebrovascular Diseases, Ministry of Education, Gannan Medical University, Ganzhou, Jiangxi 341000, China; The First School of Clinical Medicine, Gannan Medical University, Ganzhou, Jiangxi 341000, China. ; Junyun Huang:1.Department of Laboratory Medicine, First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi 341000, China. ; Furong Qing:1.Center for Immunology, Key Laboratory of Prevention and Treatment of Cardiovascular and Cerebrovascular Diseases, Ministry of Education, Gannan Medical University, Ganzhou, Jiangxi 341000, China; School of Basic Medicine, Gannan Medical University, Ganzhou, Jiangxi 341000, China. ; Tianfu Guo:1.School of Basic Medicine, Gannan Medical University, Ganzhou, Jiangxi 341000, China. ; Sijia Ouyang:1.School of Basic Medicine, Gannan Medical University, Ganzhou, Jiangxi 341000, China. ; Lu Xie:1.School of Basic Medicine, Gannan Med
期刊:The rare-earth yttrium induces cell apoptosis and autophagy in the male reproductive system through ROS-Ca2+-CamkII/Ampk axis
DOI:10.1016/j.ecoenv.2023.115262
影响因子 :6.8
引用产品: TM4 细胞 , TM3 细胞 , GC-1 spg 细胞 , GC-2spd (ts) 细胞 , DMEM/F12 培养基 , DMEM高糖 培养基
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X-box binding protein 1 caused an imbalance in pyroptosis and mitophagy in immature rats with di-(2-ethylhexyl) phthalate-induced testis toxicity (2023-03-27)
作者:Yifan Hong:1.Department of Urology, Children's Hospital of Chongqing Medical University, Chongqing 400014, China. ; 2.Pediatric Research Institute, Children's Hospital of Chongqing Medical University, Chongqing 400014, China. ; 3.Chongqing Key Laboratory of Children Urogenital Development and Tissue Engineering, Children's Hospital of Chongqing Medical University, Chongqing 400014, China. ; 4.Chongqing Key Laboratory of Pediatrics, Children's Hospital of Chongqing Medical University, Chongqing 400014, China. ; 5.Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing 400014, China. ; 6.National Clinical Research Center for Child Health and Disorders, Children's Hospital of Chongqing Medical University, Chongqing 400014, China. ; 7.China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children's Hospital of Chongqing Medical University, Chongqing 400014, Chi
期刊:X-box binding protein 1 caused an imbalance in pyroptosis and mitophagy in immature rats with di-(2-ethylhexyl) phthalate-induced testis toxicity
DOI:10.1016/j.gendis.2023.02.030
影响因子 :6.8
引用产品: GC-1 spg 细胞 , GC-2spd (ts) 细胞
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N-acetylcysteine rescues meiotic arrest during spermatogenesis in mice exposed to BDE-209 (2023-02-18)
作者:Yu Wei:1.Department of Occupational and Environmental Health, School of Public Health, Anhui Medical University, Meishan Rd 81, Hefei, 230032, China. ; Wenfeng Geng:1.Department of Occupational and Environmental Health, School of Public Health, Anhui Medical University, Meishan Rd 81, Hefei, 230032, China. ; 2.Department of Health Supervision, Administrative Committee of Hefei Xinzhan High-Tech Industrial Development Zone, Wenzhong Rd 999, Hefei, 230000, China. ; Taifa Zhang:1.Department of Occupational and Environmental Health, School of Public Health, Anhui Medical University, Meishan Rd 81, Hefei, 230032, China. ; Huan He:1.Department of Occupational and Environmental Health, School of Public Health, Anhui Medical University, Meishan Rd 81, Hefei, 230032, China. ; Jinxia Zhai:1.Department of Occupational and Environmental Health, School of Public Health, Anhui Medical University, Meishan Rd 81, Hefei, 230032, China. jinxia.zhai@foxmail.com. ;
期刊:N-acetylcysteine rescues meiotic arrest during spermatogenesis in mice exposed to BDE-209
DOI:10.1007/s11356-023-25874-0
影响因子 :5.8
引用产品: GC-2spd (ts) 细胞
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Curcumin attenuates diphenyl phosphate-induced apoptosis in GC-2spd(ts) cells through activated autophagy via the Nrf2/P53 pathway (2023-12-14)
作者:Lai Wei:1.Department of Health Toxicology, Xiangya School of Public Health, Central South University, Changsha, People's Republic of China. ; Siwen Li:1.Department of Health Toxicology, Xiangya School of Public Health, Central South University, Changsha, People's Republic of China. ; Yu Ma:1.Department of Health Toxicology, Xiangya School of Public Health, Central South University, Changsha, People's Republic of China. ; Shuzi Ye:1.Department of Health Toxicology, Xiangya School of Public Health, Central South University, Changsha, People's Republic of China. ; Yu Yuan:1.Department of Health Toxicology, Xiangya School of Public Health, Central South University, Changsha, People's Republic of China. ; Yuan Zeng:1.Department of Health Toxicology, Xiangya School of Public Health, Central South University, Changsha, People's Republic of China. ; Tausif Raza:1.Department of Health Toxicology, Xiangya School of Public Health, Central South University, Changsha, People's Republic of Chi
期刊:Curcumin attenuates diphenyl phosphate-induced apoptosis in GC-2spd(ts) cells through activated autophagy via the Nrf2/P53 pathway
影响因子 :4.5
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The crosstalk between M1 macrophage polarization and energy metabolism disorder contributes to polystyrene nanoplastics-triggered testicular inflammation (2023-08-26)
作者:Siwen Li:1.Xiangya School of Public Health, Central South University, Changsha, 410078, PR China. ; Lemei Liu:1.Xiangya School of Public Health, Central South University, Changsha, 410078, PR China. ; Gang Luo:1.Xiangya School of Public Health, Central South University, Changsha, 410078, PR China. ; Yu Yuan:1.Xiangya School of Public Health, Central South University, Changsha, 410078, PR China. ; Die Hu:1.Xiangya School of Public Health, Central South University, Changsha, 410078, PR China. ; Fang Xiao:1.Xiangya School of Public Health, Central South University, Changsha, 410078, PR China. Electronic address: fangxiao@csu.edu.cn. ;
期刊:The crosstalk between M1 macrophage polarization and energy metabolism disorder contributes to polystyrene nanoplastics-triggered testicular inflammation
影响因子 :4.3
引用产品: RAW 264.7 细胞 , GC-2spd (ts) 细胞 , Annexin V-FITC/ PI荧光双染细胞凋亡检测试剂盒
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Epigallocatechin gallate alleviates mono-2-ethylhexyl phthalate-induced male germ cell pyroptosis by inhibiting the ROS/mTOR/NLRP3 pathway (2023-06-05)
作者:Yifan Hong:1.Department of Urology, Children's Hospital of Chongqing Medical University, Chongqing 400014, PR China; Pediatric Research Institute, Children's Hospital of Chongqing Medical University, Chongqing 400014, PR China; Chongqing Key Laboratory of Children Urogenital Development and Tissue Engineering, Chongqing Key Laboratory of Pediatrics, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children's Hospital of Chongqing Medical University, Chongqing 400014, PR China. ; Xiazhu Zhou:1.Department of Urology, Children's Hospital of Chongqing Medical University, Chongqing 400014, PR China; Pediatric Research Institute, Children's Hospital of Chongqing Medical University, Chongqing 400014, PR China; Chongqing Key Laboratory of Children Urogenital Development and Tissue Engineering, Chongqi
期刊:Epigallocatechin gallate alleviates mono-2-ethylhexyl phthalate-induced male germ cell pyroptosis by inhibiting the ROS/mTOR/NLRP3 pathway
影响因子 :3.2
引用产品: GC-1 spg 细胞 , GC-2spd (ts) 细胞
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BDE-209 induce spermatocytes arrest at early-pachytene stage during meiotic prophase I in mice (2012-01-20)
作者:Jinxia Zhai:1.Department of Occupational and Environmental Health, School of Public Health, Anhui Medical University, Meishan Rd 81, Hefei, 230032, China. Electronic address: jinxia.zhai@foxmail.com. ; Wenfeng Geng:1.Department of Occupational and Environmental Health, School of Public Health, Anhui Medical University, Meishan Rd 81, Hefei, 230032, China. Electronic address: 2456096589@qq.com. ; Taifa Zhang:1.Department of Occupational and Environmental Health, School of Public Health, Anhui Medical University, Meishan Rd 81, Hefei, 230032, China. Electronic address: 1985561058@qq.com. ; Yu Wei:1.Department of Occupational and Environmental Health, School of Public Health, Anhui Medical University, Meishan Rd 81, Hefei, 230032, China. Electronic address: 792393561@qq.com. ; Huan He:1.Department of Occupational and Environmental Health, School of Public Health, Anhui Medical University, Meishan Rd 81, Hefei, 230032, China. Electronic address: 1943471922@qq.com. ; Wenjing Chen:1.Dep
期刊:BDE-209 induce spermatocytes arrest at early-pachytene stage during meiotic prophase I in mice
影响因子 :4.5
引用产品: GC-2spd (ts) 细胞
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