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产品概述
| 名称 | GC-2spd(ts) (小鼠精母细胞) (STR鉴定正确) |
| 别称 | GC-2 |
| 种属 | 小鼠 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:3-1:6 |
| 推荐换液频率 | 2-3次/周 |
| 背景描述 | The cells have lost their differentiation potential, and are currently arrested at a premeiotic stage. |
| 年龄（性别） | 雄性；6周龄 |
| 组织来源 | 精母细胞；SV40大T抗原转染 |
| 细胞类型 | 条件永生化细胞系 |
| 生物安全等级 | BSL-2 |
| 应用领域 | This cell line may be useful for the study of spermatogenesis. |
| 保藏机构 | ATCC; CRL-2196 BCRC; 60313 |
STR鉴定
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STR位点信息
18-3 16,17 6-7 17 5-5 14,17,18,19 X-1 28 1-2 13,19 7-1 26.2,29 8-1 16 1-1 17 19-2 13 15-3 22.3 12-1 17 6-4 18 4-2 20.3 3-2 14 2-1 16 13-1 15.2,17.1
11-2 16 17-2 15 -
STR鉴定图
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参考文献
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Gut-Derived Exosomes Mediate the Microbiota Dysbiosis-Induced Spermatogenesis Impairment by Targeting Meioc in Mice (2024-03-25)
期刊：Advanced Science
影响因子 ：15.1
引用产品： GC-1 spg 细胞 , GC-2spd (ts) 细胞
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The necroptosis-mediated imbalance of mitochondrial dynamics is involved in DEHP-induced toxicity to immature testes via the PGAM5–DRP1 interaction (2024-02-15)
期刊：ENVIRONMENTAL POLLUTION
DOI：10.1016/j.envpol.2024.123585
影响因子 ：8.9
引用产品： GC-1 spg 细胞 , GC-2spd (ts) 细胞
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Roles of ferroptosis in type 1 diabetes induced spermatogenic dysfunction (2024-02-17)
期刊：FREE RADICAL BIOLOGY AND MEDICINE
DOI：10.1016/j.freeradbiomed.2024.02.006
影响因子 ：7.4
引用产品： GC-2spd (ts) 细胞
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Dapagliflozin ameliorates diabetes-induced spermatogenic dysfunction by modulating the adenosine metabolism along the gut microbiota-testis axis (2024-01-05)
期刊：Scientific Reports
DOI：10.1038/s41598-024-51224-2
影响因子 ：4.6
引用产品： GC-2spd (ts) 细胞
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The gut metabolite 3-hydroxyphenylacetic acid rejuvenates spermatogenic dysfunction in aged mice through GPX4-mediated ferroptosis (2023-09-27)
期刊：Microbiome
DOI：10.1186/s40168-023-01659-y
影响因子 ：15.5
引用产品： GC-2spd (ts) 细胞
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The rare-earth yttrium induces cell apoptosis and autophagy in the male reproductive system through ROS-Ca2+-CamkII/Ampk axis (2023-07-20)
期刊：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
DOI：10.1016/j.ecoenv.2023.115262
影响因子 ：6.8
引用产品： TM4 细胞 , TM3 细胞 , GC-1 spg 细胞 , GC-2spd (ts) 细胞 , DMEM/F12 培养基 , DMEM高糖 培养基
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X-box binding protein 1 caused an imbalance in pyroptosis and mitophagy in immature rats with di-(2-ethylhexyl) phthalate-induced testis toxicity (2023-03-27)
期刊：Genes & Diseases
DOI：10.1016/j.gendis.2023.02.030
影响因子 ：6.8
引用产品： GC-1 spg 细胞 , GC-2spd (ts) 细胞
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N-acetylcysteine rescues meiotic arrest during spermatogenesis in mice exposed to BDE-209 (2023-02-18)
期刊：ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
DOI：10.1007/s11356-023-25874-0
影响因子 ：5.8
引用产品： GC-2spd (ts) 细胞
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Curcumin attenuates diphenyl phosphate-induced apoptosis in GC-2spd(ts) cells through activated autophagy via the Nrf2/P53 pathway (2023-12-14)
期刊：ENVIRONMENTAL TOXICOLOGY
影响因子 ：4.5
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The crosstalk between M1 macrophage polarization and energy metabolism disorder contributes to polystyrene nanoplastics-triggered testicular inflammation (2023-08-26)
期刊：FOOD AND CHEMICAL TOXICOLOGY
影响因子 ：4.3
引用产品： RAW 264.7 细胞 , GC-2spd (ts) 细胞 , Annexin V-FITC/ PI荧光双染细胞凋亡检测试剂盒
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Epigallocatechin gallate alleviates mono-2-ethylhexyl phthalate-induced male germ cell pyroptosis by inhibiting the ROS/mTOR/NLRP3 pathway (2023-06-05)
期刊：TOXICOLOGY IN VITRO
影响因子 ：3.2
引用产品： GC-1 spg 细胞 , GC-2spd (ts) 细胞
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BDE-209 induce spermatocytes arrest at early-pachytene stage during meiotic prophase I in mice (2012-01-20)
期刊：Toxicology
影响因子 ：4.5
引用产品： GC-2spd (ts) 细胞
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