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产品概述
名称 | TM3 (小鼠睾丸间质细胞) (STR鉴定正确) |
别称 | TM-3 |
种属 | 小鼠 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:3-1:8 |
推荐换液频率 | 2-3次/周 |
注意事项 | 该细胞贴壁松散,操作时请尽量轻柔;换液时需预热培养基;收货如有大块脱落的细胞团,为正常现象,请按照收货注意事项处理。 |
背景描述 | TM3细胞在LH作用下cAMP产量升高,但对促卵泡激素没有响应。TM3细胞对LH的响应持续时间与血清批次有关;在LH存在下,TM3细胞可以代谢胆固醇。 |
年龄(性别) | 雄性;11-13天 |
组织来源 | 睾丸间质 |
细胞类型 | 自发永生化细胞 |
生物安全等级 | BSL-1 |
致瘤性 | No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium. |
受体表达情况 | Luteinizing hormone (LH); epidermal growth factor (EGF); androgen receptor, positive; estrogen receptor, positive; progesterone receptor, positive |
基因表达情况 | prostaglandin F2a |
保藏机构 | ATCC; CRL-1714 ECACC; 91060526 |
STR鉴定
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STR位点信息
18-3 18 6-7 15 5-5 11,12 X-1 24 1-2 15,17 7-1 25.2,26.2 8-1 13,16,17 1-1 16 19-2 9 15-3 20.3,22.3 12-1 16 6-4 16.3,18 4-2 19.3 3-2 13,14 2-1 9,16 13-1 16.2
11-2 15,18 17-2 15,16 -
STR鉴定图
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参考文献
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Anti-Cancer Potency of Copper-Doped Carbon Quantum Dots Against Breast Cancer Progression (2024-02-26)
期刊:International Journal of Nanomedicine
影响因子 :8.0
引用产品: NRK-52E 细胞 , TM3 细胞 , MA-782细胞专用培养基 , TM3细胞专用培养基 , DMEM高糖完全培养基 (含10%FBS)
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Melatonin alleviates oxidative stress damage in mouse testes induced by bisphenol A (2024-02-19)
期刊:Frontiers in Cell and Developmental Biology
DOI:10.3389/fcell.2024.1338828
影响因子 :5.5
引用产品: TM3 细胞
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Toxic effects of Tripterygium glycoside tablets on the reproductive system of male rats by metabolomics, cytotoxicity, and molecular docking (2023-04-10)
期刊:PHYTOMEDICINE
DOI:10.1016/j.phymed.2023.154813
影响因子 :7.9
引用产品: TM3细胞专用培养基 , TM4细胞专用培养基 , TM3 细胞 , TM4 细胞
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1-Nitropyrene disrupts testicular steroidogenesis via oxidative stress-evoked PERK-eIF2α pathway (2023-05-17)
期刊:ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
DOI:10.1016/j.ecoenv.2023.115027
影响因子 :6.8
引用产品: TM3 细胞 , TM3细胞专用培养基
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The rare-earth yttrium induces cell apoptosis and autophagy in the male reproductive system through ROS-Ca2+-CamkII/Ampk axis (2023-07-20)
期刊:ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
DOI:10.1016/j.ecoenv.2023.115262
影响因子 :6.8
引用产品: TM4 细胞 , TM3 细胞 , GC-1 spg 细胞 , GC-2spd (ts) 细胞 , DMEM/F12 培养基 , DMEM高糖 培养基
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Triphenyl phosphate induced apoptosis of mice testicular Leydig cells and TM3 cells through ROS-mediated mitochondrial fusion inhibition (2023-04-05)
期刊:ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
DOI:10.1016/j.ecoenv.2023.114876
影响因子 :6.8
引用产品: TM3 细胞
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Phytochemical analysis and in vitro and in vivo antioxidant properties of Plagiorhegma dubia Maxim as a medicinal crop for diabetes treatment (2023-03-09)
期刊:Arabian Journal of Chemistry
DOI:10.1016/j.arabjc.2023.104788
影响因子 :6.0
引用产品: TM3 细胞
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Prenatal DEHP Exposure Induces Premature Testicular Aging by Promoting Leydig Cell Senescence through the MAPK Signaling Pathways (2023-05-28)
期刊:Advanced Biology
影响因子 :3.7
引用产品: TM3 细胞
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Rosmarinic acid ameliorates autoimmune responses through suppression of intracellular nucleic acid-mediated type I interferon expression (2023-05-26)
期刊:BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
DOI:10.1016/j.bbrc.2023.05.113
影响因子 :3.1
引用产品: TM3 细胞
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Knockdown of ZnT4 Induced Apoptosis, Inhibited Proliferation and testosterone synthesis of TM3 cells (2023-09-21)
期刊:IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
DOI:10.1007/s11626-023-00804-z
影响因子 :2.1
引用产品: TM3 细胞 , TM3细胞专用培养基
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MicroRNA profiling reveals the role of miR-133b-3p in promoting apoptosis and inhibiting cell proliferation and testosterone synthesis in mouse TM3 cells (2023-01-30)
期刊:IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
DOI:10.1007/s11626-022-00745-z
影响因子 :2.1
引用产品: TM3 细胞
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Melatonin inhibits autophagy in TM3 cells via AKT/FOXO1 pathway (2001-02-20)
期刊:Molecular Biology Reports
DOI:10.1007/s11033-021-07107-0
影响因子 :2.8
引用产品: TM3 细胞
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Prenatal exposure to environmentally relevant levels of PBDE-99 leads to testicular dysgenesis with steroidogenesis disorders (2010-01-20)
期刊:Journal of Hazardous Materials
DOI:10.1016/j.jhazmat.2021.127547
影响因子 :14.2
引用产品: TM3 细胞 , TM3细胞专用培养基
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1,25-Dihydroxyvitamin D deficiency accelerates male reproductive senescence in aging mice and 1,25(OH)2D3 alleviates oxidative stress via NF-κB/SOD (2004-01-20)
期刊:American Journal Of Physiology-Endocrinology And Metabolism
DOI:10.1152/ajpendo.00531.2020
影响因子 :5.1
引用产品: TM3 细胞
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A Protein from Dioscorea polystachya (Chinese Yam) Improves Hydrocortisone-Induced Testicular Dysfunction by Alleviating Leydig Cell Injury via Upregulation of the Nrf2 Pathway (2011-01-20)
期刊:Oxidative Medicine and Cellular Longevity
影响因子 :0.0
引用产品: TM3 细胞 , TM3细胞专用培养基
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Oxidized-LDL inhibits testosterone biosynthesis by affecting mitochondrial function and the p38 MAPK/COX-2 signaling pathway in Leydig cells (2008-00-20)
期刊:Cell Death & Disease
DOI:10.1038/s41419-020-02751-z
影响因子 :9.0
引用产品: TM3 细胞
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Melatonin inhibits apoptosis in mouse Leydig cells via the retinoic acid-related orphan nuclear receptor α/p53 pathway (2002-00-20)
期刊:Life Sciences
影响因子 :6.1
引用产品: TM3 细胞
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BMAL1 knockdown promoted apoptosis and reduced testosterone secretion in TM3 Leydig cell line (2004-00-20)
期刊:Gene
DOI:10.1016/j.gene.2020.144672
影响因子 :3.5
引用产品: TM3 细胞
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Melatonin Inhibits Apoptosis and Oxidative Stress of Mouse Leydig Cells via a SIRT1-Dependent Mechanism (2008-09-20)
期刊:Molecules.
影响因子 :4.6
引用产品: TM3 细胞
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