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产品概述
名称 | GC-1 spg (小鼠精原细胞) (种属鉴定正确) |
别称 | GC-1spg; GC-1; GC1-SPG |
种属 | 小鼠 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:4-1:6 |
推荐换液频率 | 2-3次/周 |
背景描述 | Type B spermatogonia were immortalized by transfection with pSV3-neo (a plasmid containing coding sequences for the SV40 large T antigen and neomycin resistance) |
年龄(性别) | 雄性;10日龄 |
组织来源 | 睾丸 |
细胞类型 | 转化细胞系 |
生物安全等级 | BSL-2 |
保藏机构 | ATCC; CRL-2053 |
STR鉴定
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STR位点信息
18-3 17,19 6-7 12,13 5-5 14 X-1 25 1-2 13 7-1 29 8-1 15 1-1 10 19-2 11,12 15-3 20.3 12-1 20 6-4 16 4-2 19.3,20.3 3-2 14 2-1 9 13-1 16.2,17.2
11-2 15,17 17-2 13,14 -
STR鉴定图
参考文献
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Gut-Derived Exosomes Mediate the Microbiota Dysbiosis-Induced Spermatogenesis Impairment by Targeting Meioc in Mice (2024-03-25)
作者:Tong Chen:1.College of Animal Sciences, Jilin University, 5333 Xian Road, Changchun, Jilin, 130062, China. ; Boqi Zhang:1.College of Animal Sciences, Jilin University, 5333 Xian Road, Changchun, Jilin, 130062, China. ; Guitian He:1.College of Animal Sciences, Jilin University, 5333 Xian Road, Changchun, Jilin, 130062, China. ; Nan Wang:1.College of Animal Sciences, Jilin University, 5333 Xian Road, Changchun, Jilin, 130062, China. ; Maosheng Cao:1.College of Animal Sciences, Jilin University, 5333 Xian Road, Changchun, Jilin, 130062, China. ; Caomeihui Shen:1.College of Animal Sciences, Jilin University, 5333 Xian Road, Changchun, Jilin, 130062, China. ; Xue Chen:1.College of Animal Sciences, Jilin University, 5333 Xian Road, Changchun, Jilin, 130062, China. ; Lu Chen:1.College of Animal Sciences, Jilin University, 5333 Xian Road, Changchun, Jilin, 130062, China. ; Kening Liu:1.College of Animal Sciences, Jilin University, 5333 Xian Road, Changchun, Jilin, 130062, China. ; Yux
期刊:Gut-Derived Exosomes Mediate the Microbiota Dysbiosis-Induced Spermatogenesis Impairment by Targeting Meioc in Mice
影响因子 :15.1
引用产品: GC-1 spg 细胞 , GC-2spd (ts) 细胞
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The necroptosis-mediated imbalance of mitochondrial dynamics is involved in DEHP-induced toxicity to immature testes via the PGAM5–DRP1 interaction (2024-02-15)
作者:Xiazhu Zhou:1.Department of Urology, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing, 400014, PR China; Chongqing Key Laboratory of Structural Birth Defect and Reconstruction, Chongqing Key Laboratory of Children Urogenital Development and Tissue Engineering, Children's Hospital of Chongqing Medical University, Chongqing, 400014, PR China. ; Yifan Hong:1.Department of Urology, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing, 400014, PR China; Chongqing Key Laboratory of Structural Birth Defect and Reconstruction, Chongqing Key Laboratory of Children Urogenital Development and Tissue Engineering, Children's Hospital of Chongqing Medical University, Chongqing, 400014, PR China. ; Jin
期刊:The necroptosis-mediated imbalance of mitochondrial dynamics is involved in DEHP-induced toxicity to immature testes via the PGAM5–DRP1 interaction
DOI:10.1016/j.envpol.2024.123585
影响因子 :8.9
引用产品: GC-1 spg 细胞 , GC-2spd (ts) 细胞
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Ferritinophagy-mediated ferroptosis of spermatogonia is involved in busulfan-induced oligospermia in the mice (2024-01-12)
作者:Jinyu Xu:1.Department of Histology and Embryology, College of Basic Medicine, Binzhou Medical University, Yantai, 246003, China; Xu Rongxiang Regenerative Medicine Research Center, Binzhou Medical University, Yantai, 264003, China. ; Lianshuang Zhang:1.Department of Histology and Embryology, College of Basic Medicine, Binzhou Medical University, Yantai, 246003, China; Xu Rongxiang Regenerative Medicine Research Center, Binzhou Medical University, Yantai, 264003, China. ; Yaru Si:1.Xu Rongxiang Regenerative Medicine Research Center, Binzhou Medical University, Yantai, 264003, China; Department of Pharmacology, College of Pharmacy, Binzhou Medical University, Yantai, 264003, China. ; Wanyue Huang:1.College of Animal Science and Technology, Anhui Agricultural University, 130 West Changjiang Road, Hefei, 230036, China. ; Ranran Liu:1.Clinical Laboratory, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, 264199, China. ; Zhiyuan Liu:1.College of Clinical Medicine, Bin Z
期刊:Ferritinophagy-mediated ferroptosis of spermatogonia is involved in busulfan-induced oligospermia in the mice
影响因子 :5.1
引用产品: GC-1 spg 细胞
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Long-term effect of PBDE-99 prenatal exposure on spermatogenic injuries via the dysregulation of autophagy (2023-03-20)
作者:Jun Wu:1.Department of Urology, Guangzhou Women and Children's Medical Center, National Children's Medical Center for South Central Region, Guangzhou Medical University, Guangzhou, Guangdong, China; Department of Urology, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China. ; Fuming Deng:1.Department of Urology, Guangzhou Women and Children's Medical Center, National Children's Medical Center for South Central Region, Guangzhou Medical University, Guangzhou, Guangdong, China. ; Xiangliang Tang:1.Department of Urology, Guangzhou Women and Children's Medical Center, National Children's Medical Center for South Central Region, Guangzhou Medical University, Guangzhou, Guangdong, China. ; Wenbin Chen:1.Department of Urology, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China. ; Rui Zhou:1.Department of Urology, Guangzhou Women and Children's Medical Center, National Children's Medical Center for South Central Region, Guangzhou Medical University,
期刊:Long-term effect of PBDE-99 prenatal exposure on spermatogenic injuries via the dysregulation of autophagy
DOI:10.1016/j.jhazmat.2023.131234
影响因子 :13.6
引用产品: GC-1 spg 细胞
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Wnt10a downregulation contributes to MEHP-induced disruption of self-renewal and differentiation balance and proliferation inhibition in GC-1 cells: Insights from multiple transcriptomic profiling (2023-06-24)
作者:Yifan Hong:1.Department of Urology, Children's Hospital of Chongqing Medical University, Chongqing, 400014, PR China; Pediatric Research Institute, Children's Hospital of Chongqing Medical University, Chongqing, 400014, PR China; Chongqing Key Laboratory of Children Urogenital Development and Tissue Engineering; Chongqing Key Laboratory of Pediatrics; Ministry of Education Key Laboratory of Child Development and Disorders; National Clinical Research Center for Child Health and Disorders; China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children's Hospital of Chongqing Medical University, Chongqing, 400014, PR China. ; Xiazhu Zhou:1.Department of Urology, Children's Hospital of Chongqing Medical University, Chongqing, 400014, PR China; Pediatric Research Institute, Children's Hospital of Chongqing Medical University, Chongqing, 400014, PR China; Chongqing Key Laboratory of Children Urogenital Development and Tissue Engineering; Ch
期刊:Wnt10a downregulation contributes to MEHP-induced disruption of self-renewal and differentiation balance and proliferation inhibition in GC-1 cells: Insights from multiple transcriptomic profiling
DOI:10.1016/j.envpol.2023.122091
影响因子 :8.9
引用产品: GC-1 spg 细胞
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Puerarin improves busulfan-induced disruption of spermatogenesis by inhibiting MAPK pathways (2023-07-27)
作者:Hai-Tao Li:1.Medical School, Institute of Reproductive Medicine, Nantong University, Nantong 226001, Jiangsu, China. ; Kun Zhong:1.Medical School, Institute of Reproductive Medicine, Nantong University, Nantong 226001, Jiangsu, China. ; Yun-Fei Xia:1.Department of Rheumatology, Affiliated Hospital of Nantong University, Nantong University, Nantong 226001, China. ; Jian Song:1.Reproductive Medicine Center, Affiliated Hospital of Nantong University, Nantong University, Nantong 226001, China. ; Xiao-Qing Chen:1.Human Resources Division and Clinical Research Center, Affiliated Hospital of Nantong University, Nantong University, Nantong 226001, China. ; Wei Zhao:1.School of Laboratory Medicine, Chengdu Medical College, Chengdu, China. Electronic address: zw198626520@126.com. ; Xu-Hui Zeng:1.Medical School, Institute of Reproductive Medicine, Nantong University, Nantong 226001, Jiangsu, China. Electronic address: zengxuhui@ntu.edu.cn. ; Tian-Xing Chen:1.Medical School, Institute of Re
期刊:Puerarin improves busulfan-induced disruption of spermatogenesis by inhibiting MAPK pathways
DOI:10.1016/j.biopha.2023.115231
影响因子 :7.5
引用产品: GC-1 spg 细胞 , TM4 细胞 , DMEM高糖 培养基 , 青霉素-链霉素溶液 (双抗),100× , 0.25%胰蛋白酶溶液 (含酚红,溶于D-Hank's)
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The rare-earth yttrium induces cell apoptosis and autophagy in the male reproductive system through ROS-Ca2+-CamkII/Ampk axis (2023-07-20)
作者:Fangpeng Hou:1.Center for Immunology, Key Laboratory of Prevention and Treatment of Cardiovascular and Cerebrovascular Diseases, Ministry of Education, Gannan Medical University, Ganzhou, Jiangxi 341000, China; The First School of Clinical Medicine, Gannan Medical University, Ganzhou, Jiangxi 341000, China. ; Junyun Huang:1.Department of Laboratory Medicine, First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi 341000, China. ; Furong Qing:1.Center for Immunology, Key Laboratory of Prevention and Treatment of Cardiovascular and Cerebrovascular Diseases, Ministry of Education, Gannan Medical University, Ganzhou, Jiangxi 341000, China; School of Basic Medicine, Gannan Medical University, Ganzhou, Jiangxi 341000, China. ; Tianfu Guo:1.School of Basic Medicine, Gannan Medical University, Ganzhou, Jiangxi 341000, China. ; Sijia Ouyang:1.School of Basic Medicine, Gannan Medical University, Ganzhou, Jiangxi 341000, China. ; Lu Xie:1.School of Basic Medicine, Gannan Med
期刊:The rare-earth yttrium induces cell apoptosis and autophagy in the male reproductive system through ROS-Ca2+-CamkII/Ampk axis
DOI:10.1016/j.ecoenv.2023.115262
影响因子 :6.8
引用产品: TM4 细胞 , TM3 细胞 , GC-1 spg 细胞 , GC-2spd (ts) 细胞 , DMEM/F12 培养基 , DMEM高糖 培养基
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X-box binding protein 1 caused an imbalance in pyroptosis and mitophagy in immature rats with di-(2-ethylhexyl) phthalate-induced testis toxicity (2023-03-27)
作者:Yifan Hong:1.Department of Urology, Children's Hospital of Chongqing Medical University, Chongqing 400014, China. ; 2.Pediatric Research Institute, Children's Hospital of Chongqing Medical University, Chongqing 400014, China. ; 3.Chongqing Key Laboratory of Children Urogenital Development and Tissue Engineering, Children's Hospital of Chongqing Medical University, Chongqing 400014, China. ; 4.Chongqing Key Laboratory of Pediatrics, Children's Hospital of Chongqing Medical University, Chongqing 400014, China. ; 5.Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing 400014, China. ; 6.National Clinical Research Center for Child Health and Disorders, Children's Hospital of Chongqing Medical University, Chongqing 400014, China. ; 7.China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children's Hospital of Chongqing Medical University, Chongqing 400014, Chi
期刊:X-box binding protein 1 caused an imbalance in pyroptosis and mitophagy in immature rats with di-(2-ethylhexyl) phthalate-induced testis toxicity
DOI:10.1016/j.gendis.2023.02.030
影响因子 :6.8
引用产品: GC-1 spg 细胞 , GC-2spd (ts) 细胞
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Melatonin Ameliorates Diquat-Induced Testicular Toxicity via Reducing Oxidative Stress, Inhibiting Apoptosis, and Maintaining the Integrity of Blood-Testis Barrier in Mice (2023-02-08)
作者:Li Yang:1.College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang 712100, China. ; Jianyong Cheng:1.College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang 712100, China. ; Dejun Xu:1.Chongqing Key Laboratory of Herbivore Science, College of Animal Science and Technology, Southwest University, Chongqing 400715, China. ; Zelin Zhang:1.College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang 712100, China. ; Rongmao Hua:1.College of Pharmacy, Shenzhen Technology University, Shenzhen 518000, China. ; Huali Chen:1.School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621000, China. ; Jiaxin Duan:1.College of Animal Science, Shanxi Agricultural University, Taiyuan 030801, China. ; Xiaoya Li:1.College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang 712100, China. ; Qingwang Li:1.College of Animal Science and Technology,
期刊:Melatonin Ameliorates Diquat-Induced Testicular Toxicity via Reducing Oxidative Stress, Inhibiting Apoptosis, and Maintaining the Integrity of Blood-Testis Barrier in Mice
影响因子 :4.6
引用产品: GC-1 spg 细胞
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Epigallocatechin gallate alleviates mono-2-ethylhexyl phthalate-induced male germ cell pyroptosis by inhibiting the ROS/mTOR/NLRP3 pathway (2023-06-05)
作者:Yifan Hong:1.Department of Urology, Children's Hospital of Chongqing Medical University, Chongqing 400014, PR China; Pediatric Research Institute, Children's Hospital of Chongqing Medical University, Chongqing 400014, PR China; Chongqing Key Laboratory of Children Urogenital Development and Tissue Engineering, Chongqing Key Laboratory of Pediatrics, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children's Hospital of Chongqing Medical University, Chongqing 400014, PR China. ; Xiazhu Zhou:1.Department of Urology, Children's Hospital of Chongqing Medical University, Chongqing 400014, PR China; Pediatric Research Institute, Children's Hospital of Chongqing Medical University, Chongqing 400014, PR China; Chongqing Key Laboratory of Children Urogenital Development and Tissue Engineering, Chongqi
期刊:Epigallocatechin gallate alleviates mono-2-ethylhexyl phthalate-induced male germ cell pyroptosis by inhibiting the ROS/mTOR/NLRP3 pathway
影响因子 :3.2
引用产品: GC-1 spg 细胞 , GC-2spd (ts) 细胞
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Effect of Low Dietary Folate on Mouse Spermatogenesis and Spindle Assembly Checkpoint Dysfunction May Contribute to Folate Deficiency-Induced Chromosomal Instability in Cultured Mouse Spermatogonia (2023-06-08)
作者:Huanhuan Ren:1.School of Life Science, Bengbu Medical College, Bengbu, China. ; 2.Department of Clinical Laboratory, Fuyang Fifth People's Hospital, Fuyang, China. ; Kaixian Wang:1.School of Life Science, Bengbu Medical College, Bengbu, China. ; Zirui Liu:1.School of Life Science, Bengbu Medical College, Bengbu, China. ; Xuansheng Zhong:1.School of Life Science, Bengbu Medical College, Bengbu, China. ; Meng Liang:1.School of Life Science, Bengbu Medical College, Bengbu, China. ; Yaping Liao:1.School of Life Science, Bengbu Medical College, Bengbu, China. ;
期刊:Effect of Low Dietary Folate on Mouse Spermatogenesis and Spindle Assembly Checkpoint Dysfunction May Contribute to Folate Deficiency-Induced Chromosomal Instability in Cultured Mouse Spermatogonia
影响因子 :3.1
引用产品: GC-1 spg 细胞
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Bud31-mediated alternative splicing is required for spermatogonial stem cell self-renewal and differentiation (2022-09-16)
作者:Junchao Qin:1.Key Laboratory of Experimental Teratology, Ministry of Education, Department of Cell Biology, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, China. ; Tao Huang:1.Center for Reproductive Medicine, Cheeloo College of Medicine, Shandong University, Jinan, China. ; Zixiang Wang:1.Key Laboratory of Experimental Teratology, Ministry of Education, Department of Cell Biology, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, China. ; Xiyu Zhang:1.Key Laboratory of Experimental Teratology, Ministry of Education, Department of Cell Biology, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, China. ; Jing Wang:1.Key Laboratory of Experimental Teratology, Ministry of Education, Department of Cell Biology, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, China. ; Qianli Dang:1.Key Laboratory of Experimental Teratolo
期刊:Bud31-mediated alternative splicing is required for spermatogonial stem cell self-renewal and differentiation
DOI:10.1038/s41418-022-01057-1
影响因子 :12.1
引用产品: GC-1 spg 细胞
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The protective effect of C-phycocyanin in male mice reproductive system (2001-02-20)
作者:Fang-Hao Yang:1.Department of genetics and cell biology, Basic medical college, Qingdao University, Qingdao, China, 266071. yfhao1996@163.com. ; Xiao-Lei Dong:1.Department of genetics and cell biology, Basic medical college, Qingdao University, Qingdao, China, 266071. yfhao1996@163.com. ; Guo-Xiang Liu:1.Department of genetics and cell biology, Basic medical college, Qingdao University, Qingdao, China, 266071. yfhao1996@163.com. ; Lei Teng:1.Department of genetics and cell biology, Basic medical college, Qingdao University, Qingdao, China, 266071. yfhao1996@163.com. ; Lin Wang:1.Department of Reproduction, The Affiliated Hospital of Qingdao University, Qingdao, China, 266000. wanglincyf@126.com. ; Feng Zhu:1.Department of genetics and cell biology, Basic medical college, Qingdao University, Qingdao, China, 266071. yfhao1996@163.com. ; Feng-Hua Xu:1.Department of genetics and cell biology, Basic medical college, Qingdao University, Qingdao, China, 266071. yfhao1996@163.com. ; Yi-
期刊:The protective effect of C-phycocyanin in male mice reproductive system
影响因子 :6.1
引用产品: GC-1 spg 细胞
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The mechanism analysis using PI3K/AKT pathway for the effects of levocarnitine in the treatment of spermatogenic dysfunction (2010-01-20)
作者:Jisheng Wang:1.Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China. ; 2.Department of Andrology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China. ; Binghao Bao:1.Department of Andrology, China-Japan Friendship Hospital, Beijing, China. ; Fanchao Meng:1.Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China. ; 2.Department of Andrology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China. ; Sheng Deng:1.Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China. ; 2.Department of Andrology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China. ; Hengheng Dai:1.Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China. ; 2.Department of Andrology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China. ; Junlong Feng:1.Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China. ; 2.Department
期刊:The mechanism analysis using PI3K/AKT pathway for the effects of levocarnitine in the treatment of spermatogenic dysfunction
影响因子 :2.5
引用产品: GC-1 spg 细胞 , 青霉素-链霉素溶液 (双抗),100×
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