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产品概述
名称 | NCI-H146 [H146] (人小细胞肺癌细胞) (STR鉴定正确) |
别称 | H146; H-146; NCIH146 |
种属 | 人类 |
生长特性 | 悬浮,聚团生长 |
细胞形态 | 淋巴母细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:2-1:3 |
推荐换液频率 | 2-3次/周 |
注意事项 | 该细胞为悬浮细胞,请注意离心收集细胞悬液;请勿直接倒掉细胞培养液。 |
背景描述 | Derived from the pleural fluid of a patient with small cell lung cancer; the cells express elevated levels of the 4 biochemical markers characteristic of SCLC: neuron-specific enolase, the brain isoenzyme of creatine kinase, L-dopa decarboxylase and bombesin-like immunoreactivity; the cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein; the cells express relatively high amounts of c-myc mRNA, but no amplification of the gene is detected. |
年龄(性别) | 男性;59岁 |
组织来源 | 肺 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肺癌细胞 |
基因表达情况 | vasopressin negative; oxytocin negative; gastrin releasing peptide negative; vimentin positive by immunostaining; keratin positive by immunostaining; neurofilament triplet protein negative; c-myb mRNA; v-fms mRNA; Ha-ras mRNA; Ki-ras mRNA; N-ras mRNA; c-raf 1 mRNA; myc+; myb+; raf+; ras+; fms+; fes - |
保藏机构 | ATCC; HTB-173 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 11 D2S1338 19 D3S1358 11 D5S818 12 D7S820 9,10 D8S1179 12,14 D13S317 12 D16S539 11 D18S51 15,17 D19S433 13,14 D21S11 30 FGA 23 PentaD 13 PentaE 12 TH01 6,9.3 TPOX 8,11 vWA 14,16 D6S1043 11,19 D12S391 18.3,25 D2S441 10,14 -
STR鉴定图
参考文献
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NDRG2 acts as a negative regulator of the progression of small-cell lung cancer through the modulation of the PTEN-AKT-mTOR signalling cascade(2024/03/25)
作者:Zhenchuan Ma, Yuefeng Ma, Jie Feng
期刊:TOXICOLOGY AND APPLIED PHARMACOLOGY
DOI:10.1016/j.taap.2024.116915
影响因子 :3.800
引用产品:CL-0693
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